LOCUS       c00382_gnlPro..         5657 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HHFKEBNM_382.
ACCESSION   c00382_gnlPro..
VERSION     c00382_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:50:07
            Original ID  :: gnl|Prokka|HHFKEBNM_382
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5657
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5657
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2381:5630]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2382..5630
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..5657
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5657
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             922..2379
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_7_arginine"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg382_1"
                     /sec_met_domain="OKR_DC_1_C (E-value: 1e-14, bitscore:
                     43.2, seeds: 113, tool: rule-based-clusters)"
                     /sec_met_domain="OKR_DC_1_7_arginine (E-value: 2.2e-127,
                     bitscore: 413.0, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYERQQHAPIYEALERFRKKRVVPFDVPGHKRGRGNPELAQLLG
                     EKCVGLDVNSMKPLDNLCHPVSVIREAEELAADAFGAAHAFLMVGGTTSAVQSMILSVC
                     KAGDKIILPRNVHKSAINALVLCGAIPVYVNPEVNAQLGISLGMEVSQVEKAMDENPDA
                     VAVLVNNPTYYGICSDLRTIVKKAHARGMKVLADEAHGTHLYFGRNLPVSGMAAGADMA
                     AVSMHKSGGSLTQSSLLLLNKSMNAEKVRQIINLTQTTSASYLLLSSLDISRRNLALRG
                     EESFEKVAGMAQYAREEINEIGGYYAYGMDLINGTSVFDFDVTKLSIYTLGNGLAGIEV
                     YDLLRDEYDIQIEFGDICNILAYISIGDRLQDIERLVGALADIERLYKKDSTGMLSGEY
                     IAPAVVASPQQAFYAEKESLPMEQAAGRISGEFVMCYPPGIPILAPGEMVTQEIVEYIL
                     YARDKGCSMQGMEDPKVEHLQVLKGGI"
     CDS             2382..3242
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synt_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg382_2"
                     /sec_met_domain="Spermine_synth (E-value: 1.2e-54,
                     bitscore: 172.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Spermine_synt_N (E-value: 1.8e-16,
                     bitscore: 48.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELWYSEFHTGNVKLSVRINRQLFSGESEFQRIDVFESEEFGRFV
                     ALDGEIVFSDKDEFIYDEMVTHVPMTVHPNVKNVLIIGGGDGGVARELIHYPQIESIDV
                     VESDKMFVDVCAEMFPDIAQGLKDERVNIYYEDGLRFLRNKKARYDLIINDSTDPLGHT
                     EGLFTKEFYGSCYKALRDDGIMVYQHGSPFYDEDEVECRKMHRKVFRSFPVSRVYQAHI
                     PTCPSGYWLFGFASKKYHPLEDFRPERFDNLNIETWYYTTNLHRGAFMLPKYVEDLLEE
                     EENSL"
     CDS             3239..4519
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg382_3"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 1.5e-44, bitscore:
                     141.1, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 7.2e-37,
                     bitscore: 114.7, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRLLVIGCGGVASVAIRKCCQNSDVFTEIMIASRTKEKCDALKE
                     KIESTTKTKIETAKVDADNAAEVAELIRAYKPDAVLNVALPYQDLTIMDACLEAGADYI
                     DTANYEAEDTEDPTWRAIYEKRCKEKGFTAYFDYSWQWAYDEKFKEAGLTALLGTGFDP
                     GVTSVFSAYALKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSANGSYWEDGH
                     WVETEPMEFKSVYDFPEVGKKDMYLLHHEEIESLAKNIPGVQRIRFFMTFGQSYLTHMK
                     CLENVGMLSTAPVEFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGVKDGKEK
                     SIYIYNVCDHQECYKEVESQAISYTTGVPAMIGSMMVVTGQWKKPGVFNVEEFDPDPYM
                     EALNKWGLPWKVCEDPELVKVWKANED"
     CDS             4509..5630
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg382_4"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 7.8e-16,
                     bitscore: 46.4, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIDQLQTPCYVIDEKKMRENLEILKKVQEDTGCKILLAQKAFSG
                     FALYPMIGKYLAGTTASGLFEARLGHEEMGKENHVFSPAYRTEDIEELDRICDHIIFNS
                     TAQLKKFKEVCTGASLGLRINPECSTQGDHAIYDPCAPGSRLGVTRENFDGECLKWIDG
                     LHFHTLCEQNADDLEKTLQAVEEKFGEFLSEVSWLNMGGGHHITREDYDVKLLEKCIQH
                     MKETYDLDIYLEPGEAVALNAGYLVTEVMDIVENEIRTLILDASAACHMPDVLEMPYRP
                     PLKDSGEAGEKAYTYRLSSCTCLAGDVIGDYSFDREIRIGDKLYFMDMAIYSMVKNNTF
                     NGMPLPDIAVMHEDGECEVIRHFGYEDFKSRLS"
ORIGIN
        1 atcgtaatcc ctggtcatga aagaaaatac gttttctcaa ggaaagaaat tgttaaaaaa
       61 taaacacagt tttccctgat aaatgaagaa aatataaaaa gcgggtaaag ctagagcgtg
      121 tctgaaaaat cattcccgca atctgcacgc ccccactttg cgggatattt cacccgaatt
      181 cagttgccgt agcccgctac ggcgccctca tccgggcaaa atatcccaca aattgtgacg
      241 cgcatcttgc agaaagcctt tttcagacac gctctagtgc aaaatatgga aaatggaatt
      301 gacaacagtt cagaagatat gttattctca tctttagtat gaaaagacac tatcgttttc
      361 atgcaaatat ttgctcacat tcagagacag tttctgaatg ttaagccata cggacaggcg
      421 gggcagttgc cgggtgggag acaccacatt attgattgag ttagaagctc aaattttata
      481 agttgattac tttataataa tcagtgcgta agcacacatc atcttgtgaa acggtcaata
      541 agagcatagc atatatttgc cagcgattga agacgacagc agtcatgaat atttcagaat
      601 acgggaacag gcacaggata cagacggatt tatacgggaa atccggtcag tatataaaga
      661 gacagagcct gagaaattct atagatcatg agagaagcag atggtgaatg aacttatctg
      721 cttctttttt tgtgcagata tagcaaatat aaaggatatg gttggagata aacgctttgc
      781 ggggatgtgt ctcacatgcg ttcgacttga atatatagag aaataattga atcattgtgc
      841 cggtttgagg cagtatacaa ttgctgaatg atagaactgt atttgccggt acatgacgac
      901 aggaaaagag gaacatgtca aatggattat gaaagacaac agcatgcgcc catatatgaa
      961 gcactggaac gtttccgaaa gaagagagtc gttccctttg atgtgccggg acataaaaga
     1021 ggaagaggaa atccggagct tgcacagttg ctgggagaga aatgtgtggg gctggatgtt
     1081 aattctatga agccgctgga caatctctgc cacccggttt cggtgatcag agaagcggag
     1141 gagcttgcag cagatgcttt tggcgcagcg catgcattcc ttatggtagg aggaaccaca
     1201 tctgcagtgc agagcatgat cctgtctgtc tgcaaggcag gggataagat cattctgccg
     1261 cgaaatgtac ataaaagtgc catcaatgcc ctggtactgt gcggtgcaat tccggtttat
     1321 gtaaacccgg aggtgaatgc gcagctgggc atttcccttg gcatggaagt cagtcaggtg
     1381 gagaaggcta tggatgagaa tccggatgcg gtggcagttc ttgtaaataa ccctacctat
     1441 tatggaatct gttcggatct gcggactatc gtaaagaagg ctcatgcaag aggaatgaag
     1501 gtgctggcag atgaggctca tggaacccat ctttatttcg gcagaaacct tcctgtgtcc
     1561 ggaatggcgg caggtgctga catggcggca gtttccatgc acaaatccgg cggaagtctg
     1621 acacagagtt cgctgctgct tttgaataag agtatgaatg cagagaaagt gcgccagatc
     1681 atcaatctga ctcagaccac aagcgcttct tatcttctgt tgtccagtct ggatatctcc
     1741 agaagaaatc tggcactcag gggagaggaa tcctttgaaa aggttgcagg gatggcgcag
     1801 tatgccagag aggagatcaa tgagatcggc ggttattatg catatggtat ggatctgatc
     1861 aatggaacca gtgtttttga ttttgatgtt acaaaactct ccatttatac actgggaaat
     1921 gggctggcag gtattgaagt ttatgatctt ctcagagatg aatatgatat tcagatcgag
     1981 tttggagata tctgtaatat tctggcttac atttccattg gagacaggct tcaggatatt
     2041 gagaggctgg taggggcatt ggctgatatt gaacgtctgt ataagaagga cagcacaggt
     2101 atgctttccg gggaatatat cgcacctgca gtggtggcgt ctccacagca ggcattttat
     2161 gcggagaaag aatctctgcc catggaacag gcggcaggca ggatcagcgg tgaatttgtt
     2221 atgtgttatc ctccgggaat cccgattctt gctcctggag aaatggtgac acaggaaatt
     2281 gtagaataca ttctttatgc aagagacaaa ggctgttcca tgcagggaat ggaggatccg
     2341 aaggtagaac atcttcaggt cctgaaaggg ggtatctgat catggaatta tggtattctg
     2401 agttccatac aggaaatgta aagctttctg tccggattaa ccgacagtta ttttcagggg
     2461 aaagtgaatt tcagagaatc gatgtgtttg agtctgaaga atttggaaga tttgtggctc
     2521 tggacggtga gatcgtattc tctgataaag atgaatttat ttatgatgaa atggtcacgc
     2581 atgtgccaat gacagtacat cccaatgtaa aaaatgttct gatcatcggc ggcggtgacg
     2641 gtggagttgc cagagaactg atccattatc cacagattga gagcattgat gtggtggaat
     2701 ccgataaaat gtttgtggat gtatgtgcgg agatgtttcc ggatattgcc cagggactga
     2761 aggatgagag agtcaacatt tattatgaag acggccttcg gttcctgcgg aataaaaagg
     2821 ccagatatga cctgatcatc aatgattcca cagatccgct gggacataca gagggacttt
     2881 ttacaaagga attctacgga agctgttata aggctctcag agatgacgga atcatggttt
     2941 atcagcatgg aagtcctttt tatgatgagg atgaggtgga atgcagaaag atgcacagaa
     3001 aggttttccg gtcatttccg gtgagcaggg tttatcaggc acatatccct acctgtccct
     3061 cgggatactg gctttttgga tttgcatcaa agaaatatca tccactggaa gatttcaggc
     3121 cggagaggtt tgataatctt aatatagaaa catggtatta tacaacaaac cttcacagag
     3181 gagcatttat gcttccaaaa tatgtagaag atttactgga agaggaggaa aacagtttat
     3241 gagcagatta ttggtaatcg gatgtggtgg ggtagcttca gtggcaattc gcaaatgctg
     3301 tcagaacagc gatgttttta cagagattat gatcgcaagc aggacaaagg aaaaatgcga
     3361 tgcactcaaa gagaagattg aaagtacaac gaagacaaaa attgagacag caaaggtaga
     3421 tgcggataat gcagcagagg tcgcagaatt gatcagagcc tataagccgg atgctgtcct
     3481 gaatgtggca cttccatatc aggatcttac catcatggat gcatgtctgg aagccggtgc
     3541 ggattatatt gatactgcca attatgaagc agaggataca gaagatccca catggcgtgc
     3601 aatctacgaa aagagatgta aagaaaaagg ctttactgca tatttcgatt attcctggca
     3661 gtgggcttat gatgagaaat ttaaggaagc aggactgact gctcttctgg gaaccggttt
     3721 tgatccgggt gttaccagtg tattcagtgc ctatgccctg aagcattatt ttgatgaaat
     3781 ccataccatt gatattctgg actgtaatgg cggtgatcat ggatatcctt ttgccacaaa
     3841 ctttaatccg gagatcaatc ttcgagaggt ttccgcaaat ggttcctact gggaagacgg
     3901 acattgggta gaaacagagc ctatggaatt taaatctgtc tacgattttc ctgaggtagg
     3961 aaagaaggat atgtatcttc ttcatcatga agagattgaa tcactggcaa aaaatattcc
     4021 gggagtacag aggatccgtt tctttatgac attcggacag agctatctga cacatatgaa
     4081 atgtctggaa aatgtgggaa tgctttctac cgcacctgtg gaatttaatg gacaggagat
     4141 cgtgccgatc cagtttttga aagctcttct gccggatcct gcatctctgg gtccgagaac
     4201 agtgggtaaa acaaatatcg gctgtatttt cacaggtgtt aaggacggaa aagagaaaag
     4261 tatttatatt tataatgtat gtgatcatca ggaatgctat aaagaggtgg aatcccaggc
     4321 aatctcctat acaacaggtg ttccggctat gatcggttcc atgatggtag tgaccggaca
     4381 gtggaagaag ccgggtgtat ttaatgtgga agaatttgat ccggatccat atatggaagc
     4441 actgaacaaa tggggacttc catggaaagt ctgcgaagat cctgaactgg taaaggtctg
     4501 gaaagccaat gaagattgat caactgcaaa ctccctgcta tgtgatcgat gagaagaaga
     4561 tgcgggagaa tctggagatt ttgaaaaaag tccaggagga taccgggtgt aagatccttc
     4621 tggcgcagaa agcgttttcc ggctttgccc tgtatcccat gatcggaaaa tatctggcag
     4681 gtaccactgc cagtggtctg tttgaggcaa ggcttggaca tgaagagatg gggaaagaga
     4741 atcatgtttt ttcccctgct taccgtacag aagatataga ggaactggat cggatctgtg
     4801 accatattat ctttaattcc acagcacagc tgaagaaatt taaagaagtg tgtaccggag
     4861 ccagtctggg actccgtatt aatccggaat gctccactca gggggatcat gcgatctatg
     4921 atccctgtgc gccgggttca aggctggggg ttaccagaga gaactttgac ggggaatgtc
     4981 tgaaatggat cgacggactg cattttcata cgctctgcga acagaatgcg gatgacctgg
     5041 agaaaacatt gcaggcggta gaggagaagt ttggagaatt tctgtctgag gtttcctggc
     5101 tgaatatggg cggcggacat catattacca gagaggatta tgatgtaaag ctcctggaaa
     5161 aatgtattca gcatatgaag gaaacctatg atctggatat ttatctggag cccggggagg
     5221 ctgttgccct gaatgccgga tatctggtga cagaggttat ggacattgtg gaaaatgaga
     5281 tcagaacgct gattttggat gcttctgcag cctgccatat gccggatgtg ctggagatgc
     5341 cctatcgtcc gcctcttaag gacagcgggg aagcagggga gaaggcatat acctaccgct
     5401 tatcttcttg cacctgcctg gcaggagatg taatcggaga ttattccttt gacagagaga
     5461 tccggatcgg ggacaagcta tattttatgg atatggcgat ttattctatg gtgaagaata
     5521 atacatttaa cggtatgccg ctgccggata ttgcggttat gcatgaggac ggagagtgtg
     5581 aggttatcag acatttcgga tacgaagact ttaagagccg gctttcctga tatctgccgg
     5641 gctatcaaag aaaagga
//
