LOCUS       c00007_gnlPro..         7334 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GJDMADOB_7.
ACCESSION   c00007_gnlPro..
VERSION     c00007_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 01:22:13
            Original ID  :: gnl|Prokka|GJDMADOB_7
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7334
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..654
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_1"
                     /sec_met_domain="Gly_radical (E-value: 3.8e-43, bitscore:
                     134.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 4.2e-31, bitscore:
                     96.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KYAKVKAIRDEDGITTDFQVEGDFPRYGNDDDRADEIATTLLSTF
                     LEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDGRKAGEPLAPGANPSYGAEQNG
                     LLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDERSENLVHVLDGYFDQGAHHLNV
                     NVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     protocluster    1..7334
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1413]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1413
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7334
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7334
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             673..1413
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.5e-29, bitscore:
                     90.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPGGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             1876..4389
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AstE_AspA"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_3"
                     /sec_met_domain="AstE_AspA (E-value: 1.8e-33, bitscore:
                     104.2, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VKDLLSEIESYWTTRAEGYSEVNHKELNGMQKGAWLEVLKGQFPE
                     KAKDEIKILDIGTGPGFFPVILAEAGYKVTAVDYTQEMLDTAKRNAGNLCERISFYKMD
                     AQNLEFEDDVFDVVISRNLTWNLKDPKRAYEEWCRVLKPGGKLLNFDANWYGYLYDEEK
                     RLSYEEDRKSVESEHLDDHYLCTDIDRMEKIALQMPLSSINRPSWDRKFLKENGFESVA
                     VDTGIWQRVWSQEEKLNYHSTPMFMISAVKEEKNVWSENDGMGDSDSGYDRKRDLEDAM
                     LCAAPGMKKSGFLRLGGGEFSLPYTVICGSHPGKTVLITAAVHGGEYVGIQAAVELADK
                     LKPEKIHGRVILVKTVCRKEFEERSGSVCPEDEKNLNRVFPGNPQGTRMDRLAYEVVQK
                     LHSAADYYIDLHSGDDYEQLTPYIYYAGCADEDVVQMSRKMAEQADVPYMVKSNVASGG
                     SYNYAAACGIPSVLIERGQMGGWSPEEVHSTRKDVRNILCALGVYDGMRSYSNYYPMEI
                     EDVRYQSASVSGLWYPAKKPGDIIKVGEYLGCVKDYEGNILETSLSDLNGVVLYQAGSL
                     QVIKDGPMIAYGSFSRRKDERKEKITNYWAKRSDSFMEQRRAELHSDMADKWLKEIGTF
                     LPDGKLRILDVGCGAGFFSILLAKLGHEVTGIDLTPDMIIHSRELAKEENASCTFEVMD
                     AENPDFPDGTFDVIVSRNLTWTLPDAARAYKEWIRVLKTGGILINADANYGADDFSDTA
                     DLPANHAHFTVGDAMMQECEEIKRQLPISSYVRPAWDLETLGKLGINRFSIDLGISSRI
                     YTKKDEFYNPTPMFLICGEKNKCNN"
     CDS             4584..5420
                     /locus_tag="ctg7_4"
                     /transl_table=11
                     /translation="MKVQKKESKQSGNPARSLILVTVCTCTAIAFAAGGRIADFNGKNK
                     VYAASENREKTFSSDADETDVLPTGIAGVVSGMNDTASPKKSVTRIGTSCEQVMVGQRI
                     KVIEDNMAEFDVSSSMESSINELDARTAALAESARMMSDEDYDTLLHIVEAEAGTEDVK
                     GRILVANVIMNRIKNKEFPDTVTEVVWQNTNGVPQFSPTYDGRINEVTVTDETREAVRQ
                     ALEGVDYSEGALFFIQKSEAESQNVSWFEKDLKRLFKYGVHEFYTYPDSAESSTGS"
     CDS             5704..7194
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_5"
                     /sec_met_domain="PALP (E-value: 2.8e-18, bitscore: 54.7,
                     seeds: 146, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVLYKSTRGKEETVTASMAILKGLSEDGGLFVPTEIPKLDVPMD
                     ELAKMSYQETAYEVMKCFLTDFTEEELKNCINNAYDEKFDTKEIAPLHEADGAYFLELY
                     HGATIAFKDMALSILPHLMTTAAKKNQVKNEIVILTATSGDTGKAAMAGFADVPGTKII
                     VFYPKHGVSPIQEKQMVTQKGNNTYVVGITGNFDDAQTAVKKMFNDKELEAELDAAGYQ
                     FSSANSINIGRLVPQIVYYVYAYASLVRQGKIKDGQEINVVVPTGNFGNILAAYYAKQM
                     GLPVHKLICASNDNKVLYDFFRTGTYDRKRDFILTTSPSMDILISSNLERLIYRIAGGD
                     AKKCAELMQSLTAGGEYTITEDMKAQLADFYGNYCTEDETAKTIAEIFKDSHYVIDTHT
                     AVAAGVYDKYVKDTNDTTPTVIASTASPYKFTRSVMEALGADTDGKDDFALADELSALS
                     GVKLPQAVETIRTAPVLHNRVVDAPDMPKAVKDILGIR"
ORIGIN
        1 aaatatgcaa aagttaaagc tatccgcgat gaagacggca ttacaacaga tttccaggtt
       61 gagggtgact tcccacgtta tggtaatgat gatgacagag cagacgaaat cgcaacgact
      121 cttctgtcta cattccttga aaaactgaaa cacatccata cttaccgtga ttccaagcca
      181 accacatcca tccttactat tacttccaac gttgtttatg gtaaggctac aggatctctt
      241 ccagatggaa gaaaagcagg tgaacctctt gcacctggtg caaatccatc ttacggtgca
      301 gagcagaacg gtcttcttgc ttccctgaac tctgttgcca agcttgacta tgaagatgct
      361 ctcgacggta tctcaaatac tcagacaatc aatcctgatg ctctgggaca cagcgatgac
      421 gagcgttcag agaatctggt acatgtactt gacggatact ttgatcaggg tgctcatcac
      481 ctcaatgtaa acgtatttgg taaagagaaa ctgatcgacg ctatggaaca tccggaaaaa
      541 gaagagtatg caaacttcac aatccgtgta tccggatatg ctgttaagtt catcgacctt
      601 acaagagagc agcagttaga cgttattgcc agaacctgcc acgacagaat gtaattaatg
      661 gatgatgata taatggataa taaacagata aaaggctatg ttcattcttt agaaagcttc
      721 gggtccgttg acggacccgg agtaagatat gtgatttttt taagcggctg tgccatgaga
      781 tgtcagttct gtcataatcc ggatacctgg aagatgaagc agggagagct ttatacggca
      841 gatgagcttc ttaagaaagc tttacgttat aagggctact ggggcagcaa aggcggtatc
      901 actgtaagcg gtggtgagcc attgcttcag atggattttc ttacagaatt ttttaagaag
      961 gcaaaggcag agggtgtgca tacgaatctt gataccagtg gaaatccctt cactgatcag
     1021 gaaccatggc attccgggtg gctggagctt atgaagtata cagatcttgt aatgcttgat
     1081 atcaagcaga ttgacgagca ggagcatatt aagcttaccg gtcattccaa taagaatatt
     1141 cttgcgatgg cgcgggaact ttctgatatg aaaaaacctg tatggatccg tcatgtgctt
     1201 gtgccgggcg gaagtgataa ggatgaatat ctgcaccggt tagctgattt tatccatact
     1261 ctttccaatg tggagagagt ggaggtgctc ccttatcata cgcttggtaa gtttaaatgg
     1321 gagaatctgg ggctttctta tccactggag ggggttaatc ctccgacgca ggaaaggatt
     1381 gataatgccc ggaagattct tggagctatc tgagtaaaaa tatatagctg tcatatcctc
     1441 tttttggggg tatggcagct ttgtttttgg tttaaaaagg ggacgcgaag caggcgtata
     1501 gttgtaccgg atacggtttc ggcatctaag agattctaag ccctcgaaaa tccgctaaaa
     1561 gcatgagacg aaaagtccgg aattactcgc tgtcgctcag acaagggact tttcgtctca
     1621 taacgcagat ttccgaggac taagaatctc taagatgcct gcaaatgcat ccggtacaac
     1681 tatacgcctg cttcgctggt tttggttaag atataataca aggtggagtg tgaattataa
     1741 gaaggacttt gtttatataa aaatgaattg agacgtgaaa tatatccccc gggagggctt
     1801 gttggagagg ggagggattt gatataattt tggtcatata tgattataaa ttgcatgcat
     1861 ggaggttggc ataaggtgaa ggatttatta tcggagattg agagttactg gactacccgg
     1921 gctgaggggt attctgaagt taatcataag gaattgaatg ggatgcagaa gggggcatgg
     1981 cttgaggtgt tgaaagggca gttcccggag aaagcgaagg atgagattaa gattttggat
     2041 atcggaacag gacctggatt ttttcctgtt attctggctg aggcgggata taaggtgact
     2101 gctgttgatt atacgcagga gatgttggat accgcgaaga ggaatgccgg taatctttgt
     2161 gagcggattt ctttttataa gatggatgct cagaatctgg aatttgagga tgatgtgttt
     2221 gatgttgtga tcagcaggaa tctgacatgg aatctgaagg atcctaagag agcttatgaa
     2281 gagtggtgtc gtgtactgaa accgggcgga aagcttctta atttcgatgc aaactggtac
     2341 ggttatcttt atgatgagga gaaacgcctg tcttatgagg aagaccgaaa gagtgtggag
     2401 agtgagcatc tggatgatca ttacctctgt acggatattg acagaatgga gaagattgca
     2461 ctgcagatgc ctctttcttc tattaacaga ccgtcctggg acaggaagtt tctgaaggag
     2521 aacgggtttg aatctgtggc agtggatacc ggaatctggc agcgggtgtg gagtcaggaa
     2581 gagaagctga attatcattc tacgccgatg tttatgatca gcgctgtgaa ggaagagaaa
     2641 aacgtctggt ccgagaatga tggaatgggt gattctgata gcggatatga caggaaaaga
     2701 gatttggagg atgccatgct gtgtgctgct ccgggtatga aaaagagtgg atttctgaga
     2761 ctgggcggag gtgagttttc gcttccttat acagtaatct gcggttctca tcccggaaag
     2821 acagttctga tcaccgctgc ggtccatgga ggagaatatg tgggaatcca ggctgctgtt
     2881 gagcttgcgg ataagctgaa accggagaag atccatggaa gagtgatttt ggtcaagact
     2941 gtgtgcagga aggaatttga ggagaggagc ggcagtgtct gcccggagga tgagaagaat
     3001 ctgaatcgtg tatttccggg gaatccgcag ggaaccagga tggaccgtct ggcgtatgag
     3061 gtggttcaga agcttcacag tgcagcagat tattatattg atcttcacag cggggatgat
     3121 tatgagcagc ttacgcctta tatttactat gccggatgtg cagatgaaga tgtggttcag
     3181 atgtcaagga agatggcaga gcaggcagat gttccctata tggtaaagtc taatgtggca
     3241 tccggtggtt cttacaatta tgctgccgca tgtggcattc ccagtgttct gatcgaacgt
     3301 ggccagatgg gaggctggag tccggaggag gttcattcta caagaaaaga tgtgcgtaat
     3361 attctgtgtg cgctgggtgt ttacgatggc atgagaagtt acagtaatta ttatcccatg
     3421 gaaattgaag atgtgcgtta tcagtctgcc agtgtatccg ggctgtggta tcctgccaaa
     3481 aaaccgggag atatcattaa ggttggagag tatcttggct gtgttaagga ttatgaggga
     3541 aatattctgg agacaagttt atccgattta aatggagtgg ttctatatca ggcgggaagt
     3601 cttcaggtta ttaaggatgg tccgatgatc gcatatggca gtttcagcag aagaaaggat
     3661 gaacgcaagg aaaagatcac aaattactgg gcgaaaagaa gcgacagttt tatggaacag
     3721 agaagagctg agcttcacag tgatatggct gataaatggc tgaaagagat cgggactttt
     3781 cttcctgatg gaaaattaag gattctggat gtgggatgcg gtgcaggctt cttttccatt
     3841 cttttagcaa aactgggaca tgaagttaca ggaatcgatc tgacaccgga tatgatcatt
     3901 cattccaggg aactggcaaa agaagagaac gcctcctgta cgtttgaagt aatggatgcg
     3961 gaaaatccgg attttcctga tggtaccttt gatgtgatcg tgtccagaaa tcttacctgg
     4021 acacttccgg atgcagcaag agcatataaa gaatggattc gtgtgctgaa aacgggagga
     4081 atcctgatca acgcagatgc caattatggt gcagatgatt tctcagatac tgctgatctg
     4141 cctgccaatc atgctcattt tacagtaggc gatgccatga tgcaggaatg cgaggaaatt
     4201 aaacgccagc ttcctatcag ttcctatgta cgccctgcct gggatctgga gacactggga
     4261 aaactgggta tcaacagatt ctccattgat ctgggtatca gcagcagaat ttatacaaag
     4321 aaagatgagt tttataatcc aacccctatg ttcctgatct gcggcgaaaa aaataaatgt
     4381 aataattgag aaaaatgaag catatattaa agggagattt cattgatgga gccttccttt
     4441 tttctttttt gcaagctaaa atgttacaaa aatatgaaaa aagcttgaca aaatcaatta
     4501 tattaaatat aattttctta taagttacag aaatgttaca accataaaaa cctgtaatta
     4561 aaaaaactca aggaatgata agtatgaaag ttcagaaaaa agagtcaaaa cagtccggca
     4621 atccggcgcg gagtctgata ctggttacag tgtgtacctg tacggcgatt gcgtttgcag
     4681 caggcggcag aatagcagat tttaatggaa aaaacaaagt atatgcagct tctgaaaata
     4741 gggagaaaac tttttcctct gatgctgacg aaacagatgt actgccaaca ggaattgcag
     4801 gagtagtatc cggaatgaat gatacagctt ctcccaaaaa atcagtcaca cgtatcggaa
     4861 cttcctgtga acaggtaatg gtcggtcaga gaattaaagt tattgaagat aatatggcag
     4921 aattcgatgt cagctcttct atggagagca gcatcaatga gttggatgca aggacagcgg
     4981 ctcttgcaga gagtgcccgg atgatgtcgg atgaggacta tgatacattg cttcatattg
     5041 tagaagctga agcaggtact gaggatgtca agggacgtat tctggttgcc aatgtgatca
     5101 tgaaccgtat aaagaataag gaatttcccg atacagtcac agaagtggta tggcagaata
     5161 ccaatggtgt tccgcagttt tcaccgactt atgacggacg tatcaatgaa gtaactgtta
     5221 cagatgagac aagagaagcc gtcagacagg ctcttgaagg tgtggattat tcagaaggag
     5281 cactgttttt tatccagaaa tctgaagctg aaagccagaa tgtctcctgg tttgagaaag
     5341 atctgaaaag gctgtttaaa tacggcgtac atgaatttta tacctatccg gattcagcag
     5401 aaagttccac aggatcgtaa taaacgatac tcttcatata actcatttaa caggattgcg
     5461 gtaatactgc tcaaaatatg gacatattct tcgggaacaa aagaatatgt ccatattttt
     5521 gatttatagt aatcaatcat aaaaagacag aaaaaagaga aataatttag gatagtattt
     5581 tgctaaagta tatgctataa tgtgctcaat tggcgaaatg aataactgta actgttcagc
     5641 ggtttattat tactgccaat atactgaaca gttacgaata actttggaaa ggcaggaaat
     5701 atcatgcagg tattatacaa aagcacaaga ggaaaagaag aaaccgtaac agcttctatg
     5761 gctattttaa aggggctttc tgaggatggc ggcttatttg tgccgacaga gattccgaag
     5821 ctggatgtcc caatggatga gctggcaaag atgagctatc aggagacagc atatgaagta
     5881 atgaaatgtt tccttactga ttttacagaa gaagaattaa agaactgcat caacaatgcc
     5941 tatgatgaaa aatttgatac aaaagagatt gcaccgcttc atgaagcaga cggagcttat
     6001 ttccttgagc tgtatcatgg tgcgaccatt gcgtttaagg atatggcatt atcaattctt
     6061 ccacatctta tgactacagc tgctaagaag aatcaggtaa aaaatgagat tgttattctt
     6121 accgctactt ccggagatac aggtaaagcg gcaatggccg gatttgcaga tgttccggga
     6181 accaagatca tcgttttcta tccgaaacat ggtgtaagtc ctatccagga gaaacagatg
     6241 gtaactcaga aaggcaacaa tacatatgtt gtgggaatta ccggaaactt tgacgatgca
     6301 cagactgccg taaagaaaat gttcaatgat aaggaactgg aagcagaact tgatgctgca
     6361 ggatatcagt tctcatctgc gaactctatc aatatcggac gtctggtacc gcagattgtt
     6421 tattatgtat atgcatatgc atcacttgta cgtcagggca agatcaaaga cggtcaggag
     6481 atcaatgtgg tagtacctac cggaaacttt ggaaatatcc tggctgctta ttatgcaaaa
     6541 cagatgggac ttccggttca caaacttatc tgtgcctcca atgataacaa ggttctgtat
     6601 gatttcttcc gcacaggaac ttatgacaga aagcgtgact ttatccttac aacctcaccg
     6661 tccatggata ttctgatctc cagcaatctg gaacgtctga tctaccgtat cgcaggcggt
     6721 gatgcaaaaa aatgtgcaga gctgatgcag tctcttactg caggcggcga gtataccatc
     6781 acagaagaca tgaaggcaca gcttgcagac ttctatggca attactgtac agaagacgaa
     6841 acagcaaaaa caatcgcaga aatcttcaaa gacagtcatt atgtgataga tacccatact
     6901 gcagttgcag caggtgttta tgacaaatat gtaaaagata ccaatgatac cacaccaact
     6961 gtcattgcat ccacagcaag cccatataaa tttacaagaa gtgtaatgga agcacttgga
     7021 gcagatacag acggtaagga tgattttgca ctggctgatg aactgagtgc gctttccgga
     7081 gtgaaacttc cacaggcagt tgagacgatc cgcacagcac cagtgcttca taaccgggtg
     7141 gtagatgcgc cggatatgcc aaaggcagta aaagatatcc ttggaatccg ataatcgtta
     7201 ctgttcactc cgttcacagt aacgtagcca aaattcattc cagattgcct gcggcaatgg
     7261 aattttggct tgtatgtctc gggattttgg catattcatg ccaaaacacc tcgcgggata
     7321 gtggtgtgtg aaca
//
