LOCUS       c00046_gnlPro..         5130 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NAGKNJKB_46.
ACCESSION   c00046_gnlPro..
VERSION     c00046_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:52:23
            Original ID  :: gnl|Prokka|NAGKNJKB_46
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5130
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5130
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[280:3224]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      281..3224
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..5130
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5130
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             281..1552
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg46_1"
                     /sec_met_domain="HGD-D (E-value: 2.2e-57, bitscore: 182.9,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIETFGHYVENLTDKKPQSARRLLKTGWEAQNLKFRYMQDKRL
                     MPADRHLANLMMDTMLRPLQKPEDSVIVSIFTPCEMMQEVGLHPYNVEGFSCYLSASKA
                     ERAFLQQAENQGIAETLCSYHKTFLGAAQKGLLPKPKCIVYTNLTCDANLLTFRTLADF
                     YQVPAFAIDVPWQPNEENVQYVADQLKELKIFLEKHTGKTISEDALKKRLACSKRTLEN
                     YKKYQQMRADRYVPSDLVSPLYAGMTNNILLGTEEEEKYTQMLLKDIKKAPAAKGKHIY
                     WMHTIPFWSDAVKQELCFSEKAQIVGCELAETCEPDFDPEKPFEAMARRMVYHSLNGSA
                     LRRIEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCDRS
                     HGGEGQTSTRLGAFLEMLGGAENE"
     CDS             1545..3224
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg46_2"
                     /sec_met_domain="HGD-D (E-value: 4e-18, bitscore: 53.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 5.3e-66, bitscore:
                     210.5, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKLDFLYILDVLHCDSACSRER
                     MAVQLKGLAKAYAQYKGTEFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTGRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGERKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQRADIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLNPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLEFKED
                     ITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTRRVGGEEKYMMTGGV
                     SKNRGLVKTLEEKLGTKLVISDKAQLCGALGAALFAADMADA"
     CDS             3682..5097
                     /locus_tag="ctg46_3"
                     /transl_table=11
                     /translation="MWEAINSFLTTVDNLVWGVPLMVLIMAGGILLTARLGLLQIRRLP
                     LALKWMFKNEEEGDGEISSFAALCTALSATIGTGNIVGVATAVCAGGPGALFWMILAAF
                     FGMATKFSEGLLAVKYRVVAEDGHSLGGPFYYIEKGMGTKWKWLAKIFAFFGVCVGLFG
                     IGTFSQVNGISSAVQNFFDPNKSNCVNIPFLGEYSWAVVISSLILAACVAAILIGGLKR
                     IATVSQIIVPFMAVIYLIFSVALILLNITEIPAAIVTVVKGAFNPSAVTGGVVGTMIVS
                     MQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVSMTGTFIDTIVICTMTGLSIVL
                     TGAWQVEGLEGVQVTTYAFQHGLPIPGQISAFVLMICLVFFAFTTILGWDYYSERCLEY
                     LTNGHKKTILTYRWLYILAVFIGPYMTVSAVWTIADIFNGLMAIPNMIALFALSGVIVK
                     ETKAFFAAEKHKM"
ORIGIN
        1 aaaaacagct gaactttgga gaaaattcgt tactgttcac tccgttcaca gtaacttagc
       61 caaaattcat tccagattga ctgcggcaat ggaattttgg cttgtatgtc tcgggatttt
      121 ggcatattca tgccaaaaca cctcgcagaa tagtggtgtg tgaacagtaa cgaaaattca
      181 acttgacttc ccaaaaagat attattaaga tattccgtgg cactatttgt gcactgacaa
      241 ttgataaatt attattacca taatgaggaa gagacagact atggagatta ttgagacatt
      301 tggtcattat gtggaaaatc tgacggataa aaagcctcag tcagcccgcc ggctgttaaa
      361 aaccggctgg gaggcgcaga atctgaagtt ccgttacatg caggacaaga gactgatgcc
      421 tgcagacagg caccttgcaa atctgatgat ggataccatg ctgcgtccac ttcagaaacc
      481 ggaagattct gttattgtaa gtatttttac tccctgtgaa atgatgcagg aggtgggatt
      541 acatccctat aatgtagaag gattttcctg ctatctttct gcaagcaaag cagagagggc
      601 gtttctgcag caggcagaga atcagggaat tgcagagaca ctgtgcagtt atcacaagac
      661 atttcttggt gcagcgcaga aaggacttct gccaaagccg aaatgtattg tttatactaa
      721 tctaacctgc gatgctaacc tgttgacgtt tcgtacactt gctgattttt accaggtacc
      781 tgcatttgct attgacgtac cgtggcagcc gaatgaggag aatgtacagt acgtagcaga
      841 tcagttaaaa gaactgaaaa tatttctgga aaagcataca ggaaaaacaa tttctgagga
      901 tgcgttgaag aaacgtcttg catgcagcaa gcggactctg gagaattata aaaaatacca
      961 gcagatgcgt gcggacagat atgtaccgtc cgatctggtt tcgccgctgt atgcgggaat
     1021 gacaaataat atcctgcttg gaaccgaaga agaggagaaa tatacccaga tgcttcttaa
     1081 ggatattaag aaagcacctg cagcaaaggg caagcatatt tactggatgc ataccattcc
     1141 gttctggtct gatgcagtaa aacaggaact ttgcttcagt gagaaagccc agatcgtggg
     1201 ctgtgaactt gcagaaacct gtgagccgga ttttgatcct gagaaaccgt ttgaagctat
     1261 ggcaagaaga atggtatatc attcgctgaa tggaagtgca ctgagaagaa tcgaagctgg
     1321 aattcgtcat gcaaaacagg ccggtgctga tggtgcggtg tggtttggac attggggctg
     1381 taagcatacc cttggtgccg cccagcttgc aaagaaaaaa ttcgaagaag ccggtctgcc
     1441 gcttctgatc ctggatggag acgggtgtga ccgaagccac ggaggagaag gacagacatc
     1501 cactaggctg ggagcttttc tggaaatgtt gggaggtgct gagaatgaat aaaacttatt
     1561 atgtctgtaa atatacccca attgaacttc tggaatctct tggcggggaa tgtgagaact
     1621 ttaatcatat gccggaggga tttgacctgg ccgatcaggt ttcacatccc aatatctgcg
     1681 gttttggaaa aacacttctg gaaggagtca tggaggggaa aatcaaggaa cttgtcctgg
     1741 tgagctgctg tgatactatc cggagtgtct atgacattct gcttgaaagc ggcaagctgg
     1801 attttctcta tattttggat gtgcttcact gtgacagtgc ctgtagcaga gagcgtatgg
     1861 ctgtacagtt aaaagggctg gcgaaggctt atgcacagta caaaggcaca gagtttgatg
     1921 ccggaaaatt ccgggctgct ttccatgcac aggagaaaat cacaaagtcc catattgcag
     1981 tgcttggcgc cagaatgggg caggaattat ttgaaatgac cagtaaagct atgcctcttc
     2041 ctgtagagaa tgatacctgc gtgcataacc gttctgtggg aaatatactt ccaccggagg
     2101 gtgcttcttt tgatgagttg atggactggt atgcaggtga gattctgggg cagattccat
     2161 gtatgcgtat gatggacccg acaggtcgta agaagcttta taatgatccg tctgttgcgg
     2221 ggattattta tcatacagtg aagttttgtg atttttacag cttcgaatat gctgagatca
     2281 agaatcatac agatgtacct cttttaaaaa tcgaatcaga ttatactatt cagagcagcg
     2341 gtcagcttct taccagactg gaagcttttg cagagagcat tcagcctgaa acactggagc
     2401 agaccataga tggagacaca aaaggagaga gaaaaatggg aaaaggttat ttcgcaggga
     2461 ttgacagtgg ttccaccagt acagatgtgg tgattttaaa taaagatcat gagattgtga
     2521 ccagcattat cctgcctaca ggagcaggag ctgctatcgg agcagaccgt gcccttgcag
     2581 aggcattgaa ggaggccgga ttgcagagag cagatattga tgcgctggtt actaccggat
     2641 acggacgtac agctattaaa aatggtgata agagtatcac agagatcacc tgtcatgcaa
     2701 gaggtgcgca tttcctgaat ccggaagtcc gtactgttat tgatattggc ggacaggaca
     2761 gcaaggtgat ccgactggat gagaacggtg cagttgcaaa ctttgttatg aatgacaaat
     2821 gtgcggcagg aaccggacga tttctggaaa tgatggcacg taccatggaa atgaatctgg
     2881 atcagatgag cgaatgtggg ctggaattca aagaagatat tacaatttcc agtatgtgta
     2941 cagtatttgc agaatcagaa gttgtatccc tgatcgccca gaataaggca acagatgata
     3001 ttgttcatgg cctgaataag gcggttgccg tgaagactgc cgcccttacc agacgtgtgg
     3061 gcggtgagga gaaatatatg atgaccggtg gtgtttctaa gaataggggt ctggtaaaga
     3121 cgctggaaga gaagcttgga acaaagctag tgatcagtga caaggctcag ctttgcggtg
     3181 cacttggcgc agcactgttt gcggctgata tggctgatgc gtaaattttt atactttttt
     3241 tataaaagtt taattttcta ttgacgagtt caaatttaag agctatagta taccacagta
     3301 acagcaaatt aacgaggtga agtttgcagg aaagaggaag ccacacaaaa ggctataccc
     3361 gccgaaggag taaaactctc aggttccctg gatgcaggga agaactgcaa atggatcgtt
     3421 tctctggaga cacccgttag atcgggggcc gaaggtgcaa ataacacgga atgctgataa
     3481 cttcccacaa ttggttatct gacccgtgtc ttgaatctct caggcaaaag gacagaggat
     3541 gctttatcat ccccgctgat tcatatgaac tatatgaaca gtattttctg aacttctgat
     3601 ttttgaaacg gtctgttatt actttgtttc ccagctgtac aatatattac actttttaaa
     3661 agaaaaagga gatttattat tatgtgggaa gcgatcaatt cttttttgac aacagtagac
     3721 aatctggtct ggggcgtgcc ccttatggtc ctgattatgg caggtggcat cctgttaact
     3781 gccagacttg gacttcttca gatacgaaga ctcccccttg cattaaaatg gatgtttaaa
     3841 aacgaagaag aaggcgacgg tgagatttcc agctttgcag ccttatgtac agcactgagt
     3901 gccacaatcg gaaccggtaa tatcgtcggt gttgcaactg ctgtctgtgc cggaggtccc
     3961 ggagctttat tctggatgat tcttgcagca ttcttcggaa tggcaacaaa attttccgaa
     4021 ggtcttcttg cagtgaaata ccgtgtggta gcagaagacg gtcacagcct tggcggtcct
     4081 ttctattata ttgagaaagg tatgggaaca aaatggaaat ggcttgcaaa gatctttgca
     4141 ttctttggtg tatgtgtagg tcttttcgga atcggtacat tcagccaggt aaatggtatt
     4201 tccagtgcag ttcagaactt ctttgatccg aataaatcaa actgcgtaaa tattccgttc
     4261 cttggagagt attcctgggc agttgtaatt tcttccctga ttcttgcagc ctgtgttgca
     4321 gcaatcctca tcggtggtct gaaacgaatt gcaacagtca gccagattat tgtaccgttt
     4381 atggcggtga tctatctgat cttcagtgtt gcgctgattc ttttaaacat tacagagatc
     4441 ccggcagcta tcgtaacagt tgtgaaggga gcctttaatc cgtcagcagt gacaggcggt
     4501 gtggtaggaa caatgatcgt atccatgcag aaaggtgtgg ctcgtggtat tttttcaaat
     4561 gaagcaggtc ttggtagcgc acctatcgca gcggcagcag ctcagacaaa agagcctgta
     4621 cgtcagggtc ttgtaagcat gactggtaca tttattgata caatcgtgat ctgtacaatg
     4681 acaggccttt ccatcgtgct gacaggtgca tggcaggtag aaggtctgga aggtgttcag
     4741 gtaacaacct atgcattcca gcatggactt ccgatccctg gacagatttc tgcatttgta
     4801 ttaatgatct gtctggtatt ctttgcattt actacaattc ttggatggga ttattacagt
     4861 gaaagatgtc tggaatatct gacaaacgga cataagaaaa caatccttac ataccgctgg
     4921 ctttacattc tggcagtatt tatcggaccg tacatgacag taagtgctgt atggacgatc
     4981 gcagatatct tcaacggatt gatggcgatt ccgaatatga tcgcattgtt cgcattaagt
     5041 ggagtgatcg taaaagaaac aaaagcgttc tttgcagcag aaaagcataa aatgtaagga
     5101 atcaaaagta attaaataac agaaaacagt
//
