LOCUS       c00223_gnlPro..         6460 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NAGKNJKB_223.
ACCESSION   c00223_gnlPro..
VERSION     c00223_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:52:23
            Original ID  :: gnl|Prokka|NAGKNJKB_223
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6460
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6460
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[53:5661]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      54..5661
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..6460
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6460
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(54..350)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_1"
                     /sec_met_domain="ALS_ss_C (E-value: 8.1e-23, bitscore:
                     69.0, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEVIKVIDFTDVFVRMKEILYIKVFNCSREDKAELFQIAETFQA
                     KVIDYGKDSLLLEFVQTATKNNAVVKLIKEEFHRIEVVHGGSVGIESISMMER"
     CDS             complement(615..1328)
                     /locus_tag="ctg223_2"
                     /transl_table=11
                     /translation="MYLSDIHTHSIASGHGTTCTISDMAKAASKKGLKLLGISDHGPGT
                     LASGTSSYFRSLPFYPRKRFGIDILYGVELNILDSAGHTDLSDELLNNLDYAIISMHRQ
                     NYRSGSVSQNTEAYINAIKHPAVKILGHCDDTHFPVDYETLARAALRENVIFEINEASL
                     APGGYRGDTRANAARILYLCQKYQLPVILSSDSHGKEHVGDFTYAEEFVHQLMFPETLI
                     LNNQLPKLKVFLQTR"
     CDS             2091..3347
                     /locus_tag="ctg223_3"
                     /transl_table=11
                     /translation="VKSTERLIFHIDVNSAFLSWESARRVSQGLPDLREIPSCVGGDPK
                     KRTGIVVAKSIPAKKYGIQTGEPVAMALRKCPNLVIVPSDFELYDKCSRAFKAICASFA
                     PVMESFSIDEVFLDMTGTSLIYPDPVAAAHELKGKIHRELGFTVNVGISTNKLLAKMAS
                     DFQKPDRVHTLFPDEIPEKMWPLPIRNLLFLGKASEQKLLNQGIRTIGDLARERASNVQ
                     YWLGEKTGHQLWQYARGIDDSPVKAVPDEAKGFSVETTFNDDITSIEQVFPILLEQCDV
                     LATRMRRKDKKCNCISVTFRTLDFRNRSHQTKLENATDLTDEIYINATRLFKEFWKGQP
                     LRLIGVALTGLTDGTYEQMSLFEDTENKERHRKLDAAMDEIRMKFGNDKITRASIMNSN
                     ARIGRKAKAQMKNEKTEEP"
     CDS             complement(3416..3925)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_4"
                     /sec_met_domain="ALS_ss_C (E-value: 2.2e-23, bitscore:
                     70.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKGMKKRWISLYVENQVGVLSKISGLFSGKSYNLDSLTVGTTED
                     PTVSRMTIATVSDDETFEQIKKQLNRMIEVIKVIDFTDVFVRMKEILYIKVFNCSKEDK
                     VELFQIAETFKAKVIDYGKDSLLLEFVQTATKNDAVVKLIKEEFHRIEVVRGGSVGIES
                     ISMMER"
     CDS             complement(3943..5661)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.3e-49, bitscore:
                     155.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQISGNKLLVKALKEEGVEYLFGYPGACTIDISDELYKQDDVKI
                     ILPRHEQALVHEADAYARTTGKVGVCLVTSGPGATNLVTGLATANYDSVPLVCFTGQVA
                     RHLIGNDAFQEVDIVGITRSITKYGVTVRRREDLGRIIKEAFYIARTGRPGPVLIDLPK
                     DVMAELGSAEYPKNVNIRGYKPNTDVHIGQLKRAIKLLNKAKRPLFLAGGGVVISRAHE
                     IFREVVEKTKVPVVTTVMGKGSIPTDHPLYIGNLGMHGAYAANMAVSNCDVLFSIGTRF
                     NDRITGKLHEFAPHAQIIHIDIDTASISRNIQVDIPIVADAKEAITKMNEYVQECSTDK
                     WLNLIKNWKEEHPLKMRPNDLLSPMDILKEINEQFENSIIVTDVGQHQMLVSQYAEITE
                     GKQMIMSGGLGTMGYGLPGGIGAKIGNPDRPVIVISGDGGVQMNIQELATAVLEELPVI
                     LCIFNNEYLGMVRQWQKLFYGKRYAMTNLRAGALSRRTEGMEYPQYTPDFIRLAESYRA
                     KGIRVTKKEEIAAAFEEAKKNTKAPTVIEFIIDPEEMVYPMIKPGGTLEDMIMDC"
ORIGIN
        1 atacaggata tagagacgtt tctatatcct gcaaaaaata ttgattatga gatttagcgt
       61 tccatcatgc tgatggattc aattcccaca cttccaccat gtaccacttc aattctgtgg
      121 aattcttcct tgatcagttt gacaactgca ttattcttgg tggctgtctg gacaaattcc
      181 agaagcaggc tgtctttgcc gtagtcaatg acttttgcct ggaaggtttc tgcaatctga
      241 aacagttctg ctttatcttc cctggagcag ttgaaaacct tgatgtagag aatttctttc
      301 attctgacaa atacatctgt gaagtcgatt accttgataa cctcgatcat gcggtttaac
      361 tattttttga tctgttcaaa ggtttcatca tcgctgactg ttgcgatagt cattcgggat
      421 actgtaggat cctcggtggt tcctacagta agactgatct atcacgagat atgtaaatgg
      481 ttaagtgagt tttgttaatt aaaatgtact aaaatgtcgt cttttttgac aagaaattat
      541 agaaaataaa caaaataaca caatgcaaaa aacaatgcat aaaataaaag aatgtccctt
      601 tgaggaacat tcttttaacg tgtctgaagg aaaactttta atttgggaag ctggttgtta
      661 aggataagag tttccggaaa catgagctgg tgaacgaatt cttcggcata ggtgaaatct
      721 ccaacatgtt cttttccgtg gctgtcgctg gacaaaataa caggaagctg atatttctga
      781 cagagataca ggattctggc tgcattggct cttgtgtctc cccgatatcc tccgggtgcg
      841 agggaagctt cgtttatttc aaaaataaca ttttcccgta aagctgctct tgccagcgtt
      901 tcataatcta cagggaaatg tgtatcgtcg cagtggccga gtatttttac agcaggatgt
      961 tttattgcat tgatgtaagc ttctgtattc tgagatacag agcctgaacg gtagttctgc
     1021 cgatgcatac tgatgatagc gtaatccagg ttgttcagca gttcatctga cagatctgta
     1081 tgtcctgcac tgtccaggat gtttagttca actccgtaaa gaatatctat gccgaatctt
     1141 tttctgggat aaaaaggaag gcttctgaaa taagaagaag ttccggaagc cagagttccc
     1201 gggccgtggt cagaaatccc aaggagtttc agtccttttt tgctggctgc ttttgccatg
     1261 tcagagatgg tacaggtggt tccgtgaccg ctggcgatag aatgtgtgtg gatgtcagaa
     1321 agatacattg tttgtctcct ttccagagcg gtttctatat atgataagag ccttacggaa
     1381 gatgcaaagc tgctgtttga tggtacattc gttatattca agtatgacag atgaggattt
     1441 aaatgtcaat gaggaaacaa aataaaaaca actgaaatac aacaaaaatc aaataaaaag
     1501 caacacaaat aaacggctga tacatgttaa attatgttga cttcgggaaa atgagaaggt
     1561 ataatggaac tatatgaaaa cacgggggct tattgctgat caatgacagc gatagcactg
     1621 tttgcttatg tgtagacagt tacttcatga gaaaggagta acatggaaaa atttcaaaca
     1681 tcagacattc ccggaattct cttatgaatg gctggaggct gaactggata ctggtatgat
     1741 tccacaaata tggacagcga agaaattgct cctaaccttt atgactaatc gataagtata
     1801 tatcgagagg aaatgaatcc tcagaattaa gtaaaaaaag ttaccgggaa caatgtgacc
     1861 ggtagcgaaa tacagacgcc gcactgccgt atggcaagtg cggcgttgtt gttcaaaact
     1921 ctgatagcag agtgaaatca aacaaatata aatacagtaa cagctcagac aagatgatgt
     1981 aaataaagac acttttttgg acaaacgagg ttcttaccct ggagagcaga tcagggtaaa
     2041 atgaaatcat cagatatgaa gagcgcaatt catatattgg aggaagaaca gtgaaaagta
     2101 cggaacgatt aatttttcat attgatgtca acagtgcatt tctttcctgg gaatcagcca
     2161 gaagggtgag ccaggggcta ccggatctca gagaaattcc ttcctgtgta ggaggagatc
     2221 caaagaaacg gacaggtatc gtggttgcca aatctatccc tgcaaaaaaa tacgggatcc
     2281 agaccggcga gccagtagcc atggctcttc ggaaatgtcc caatctggtg atcgttccca
     2341 gtgattttga attatacgat aaatgttccc gggcatttaa agcaatctgt gcttcctttg
     2401 cacctgtgat ggaatctttt tccattgatg aggtatttct ggatatgaca ggtacttctc
     2461 tgatctatcc tgaccctgta gctgctgctc atgagctgaa aggcaagatt cacagagagc
     2521 tgggatttac agtaaatgta ggaatttcta ccaataagct tttggcaaag atggcatcag
     2581 attttcaaaa gccggacaga gtacacactc tgttcccgga tgaaatcccg gagaaaatgt
     2641 ggcctcttcc tatccgtaat ctgctgtttc tgggaaaagc ttccgagcag aagcttctga
     2701 atcagggaat ccgtactatc ggtgatctgg caagagaaag agcatccaat gtgcagtact
     2761 ggcttgggga aaaaacggga catcagctat ggcaatatgc cagaggtatc gatgattccc
     2821 ctgtaaaagc agtgcctgat gaggcaaaag gtttcagcgt ggaaactact tttaacgatg
     2881 atattacttc catagagcag gtgtttccca tacttttgga gcagtgtgat gttctggcaa
     2941 ccagaatgcg cagaaaagat aagaaatgca actgtatttc cgtgactttc cgtactttgg
     3001 atttcagaaa ccgttcacac cagacaaagc tggaaaatgc tacagatctt acagatgaaa
     3061 tttatataaa tgctacaaga ctttttaaag aattctggaa aggacagccg ctacgtctga
     3121 ttggtgtggc actgacaggc cttacagatg gcacttatga acagatgtct ctgtttgaag
     3181 atacagaaaa taaagaacgc cacagaaagc tggatgcagc tatggatgag atccggatga
     3241 aatttggaaa tgataaaatc acccgtgcca gtattatgaa cagcaatgca cggattgggc
     3301 gaaaagcgaa agcacagatg aaaaacgaaa agacggaaga accgtaagta aagagaggac
     3361 tgagcattaa gaacaatgcg acaatcctcc ctttataaaa atttcagaaa aagttttatc
     3421 gttccatcat actgatagat tcgattccta cacttcctcc acgtaccact tcaattctgt
     3481 gaaattcttc cttgatcagt ttcactactg cgtcattctt ggttgctgtc tggacaaatt
     3541 ccaggagcag gctgtctttg ccatagtcaa tgacctttgc cttgaaggtt tcggcaatct
     3601 gaaacagttc aaccttatct tctttggaac agttaaaaac cttaatatac agaatctctt
     3661 tcatgcggac aaatacgtct gtgaaatcaa taaccttaat aacctcgatc atacggttca
     3721 actgtttttt gatctgctca aaagtttcat catcactgac agttgcgatc gtcattcgag
     3781 atactgtggg gtcctcggtg gttcctacag taagactgtc cagattgtag ctttttccgg
     3841 agaaaagtcc ggagattttg gacagaacac ctacctggtt ctcaacatag agagaaatcc
     3901 atcttttttt cattccttta tccataataa ttttcctcct gatcaacagt ccataatcat
     3961 atcttccaga gttccgcctg gtttgatcat aggataaacc atttcctcag ggtcaatgat
     4021 gaattcaatt actgtaggtg cctttgtgtt cttttttgct tcctcgaagg ctgctgcaat
     4081 ttcctctttt tttgtgacac ggattccttt tgccctgtag ctctctgcaa gtctgatgaa
     4141 atccggtgtg tactgtggat attccatgcc ttcagtgcgg cgggaaagtg ctcctgcgcg
     4201 cagattggtc atagcatagc gtttgccgta gaaaagcttc tgccactggc gtaccattcc
     4261 aagatactca ttgttaaaga tacacaggat aaccggaagc tcctccagaa ctgcagttgc
     4321 aagctcctga atgttcatct gcacaccgcc gtcaccggag ataacgatga caggtctgtc
     4381 gggatttccg attttggctc cgatgccgcc tggaagacca tagcccattg ttcccagacc
     4441 accggacatg atcatctgtt tgccctctgt gatttccgca tactgggaga caagcatctg
     4501 atgctgtcct acatctgtta cgataatact gttctcaaat tgttcgttga tctctttcag
     4561 gatatccatc ggacttaaaa gatcatttgg acgcatttta agcggatgct cttctttcca
     4621 gtttttgatc aggttaagcc acttgtccgt tgaacattcc tggacgtatt cgttcatttt
     4681 ggtgatagcc tcttttgcat cagcaacaat ggggatatct acctgaatgt ttcgggaaat
     4741 ggatgctgta tcaatatcaa tgtggataat ctgggcatgt ggtgcaaatt catgaagttt
     4801 tccggtgatt ctgtcattga atctggttcc gatggagaac agcacatcgc agttgctgac
     4861 tgccatattt gctgcatagg caccatgcat gccaaggttt ccgatataga gtggatggtc
     4921 tgtggggata gagccttttc ccattacggt tgtgacaacc ggtacttttg tcttttccac
     4981 tacttcccgg aaaatttcat gggcgcggga gattactaca ccgccgcctg ccaggaataa
     5041 gggacgtttt gctttattta aaagtttgat ggcacgtttg agctggccga tgtggacatc
     5101 tgtgttgggc ttgtatccgc ggatatttac atttttagga tattcagcgc tgccgagttc
     5161 tgccattacg tcctttggaa ggtcgataag gacaggtccc ggacggccgg tgcgggcaat
     5221 gtagaaggct tctttgatga tacgtcccag gtcttcacgt ctgcggacag taacgccgta
     5281 tttggtgatg cttctggtga ttcctacgat gtctacttcc tgaaatgcat cgttgccgat
     5341 gaggtggcgg gctacctggc cggtgaagca tacaagaggt acgctgtcat agttggcggt
     5401 tgcaagtcct gttacaaggt tggttgctcc gggaccgctg gttaccagac atacgccgac
     5461 tttgccggtg gttctggcgt aggcgtcggc ttcgtggaca agggcctgtt cgtgacgggg
     5521 gaggatgatt ttgacatcgt cctgtttgta gagttcatca ctgatgtcga tggtacaggc
     5581 tccggggtag ccgaagaggt attctacgcc ttcttctttt aaggctttga ctaagagttt
     5641 gttgccggat atttgtttca tattttggta aacctccttt gtgtatgtgt ataggacgct
     5701 cagaaattta tttgtttggt gtgcggtttc ggagcctagt agatctaagt gtctgaaaat
     5761 ctgctaaaag cgttcagaaa aatctcaaac tcgctgcgct cagacagtga gatttttctg
     5821 aacaacgcag atttccaggc actaagatct acacgactcc tgcaaatgca caccaaacaa
     5881 ataaatttct gagctgtgta ttggctgata caggggcaga atggcgctgc gctgattctg
     5941 ctgagaacgc tgtaaagcag ctcccacaat tctgaatata gtaaaaaacg ccctgggctt
     6001 gtttgcccag ggcgaaaatg tttccgcggt accacctgta ttcagagtct ggctctgcac
     6061 tttgtcagta ctgatatact gtgttcctgt aacgtggaac tgacgttgaa gcctacttgg
     6121 catgtctgtc tggatctatg gatcatcaga ctggatatgc ggtttggttc actgctcggg
     6181 ggctacttcc acaattcgtt cacgaaagct cgcaccgact gctttctctc tgtgcttccc
     6241 ggggttgtgt actcctccct gtcatggcat ttggctgata tagggatatt cattgtccga
     6301 atagggtgga caaatgtttt cttgggttac attatacatt tatagagagg aaattgtcaa
     6361 ccgggttttg gataattgct gtaaaattta tgttcctgaa tgaattttgg ctacgttact
     6421 gagaacggag tgaacagtaa tgtaattgac agatttctat
//
