LOCUS       c00186_gnlPro..         3380 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CANMOLPM_186.
ACCESSION   c00186_gnlPro..
VERSION     c00186_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:32:49
            Original ID  :: gnl|Prokka|CANMOLPM_186
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3380
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3380
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1371:3378]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1372..3378
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3380
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3380
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..908)
                     /locus_tag="ctg186_1"
                     /transl_table=11
                     /translation="VKDLLSEIESYWTTRAEGYSEVNHKELNGMQKGAWLEVLKGQFPE
                     KAKDEIKILDIGTGPGFFPVILAEAGYKVTAVDYTQEMLDTAKRNAGNLCERISFYKMD
                     AQNLEFEDDVFDVVISRNLTWNLKDPKRAYEEWCRVLKPGGKLLNFDANWYGYLYDEEK
                     RLSYEEDRKSVESEHLDDHYLCTDIDRMEKIALQMPLSSINRPSWDRKFLKENGFESVA
                     VDTGIWQRVWSQEEKLNYHSTPMFMISAANEQKNVWSENDGMGDSDSGYDRNRDLEDAM
                     LCAAPCMKKNGFLRLCGGEFS"
     CDS             complement(1372..2112)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-28, bitscore:
                     87.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPDGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             complement(2131..3378)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_3"
                     /sec_met_domain="Gly_radical (E-value: 9.2e-43, bitscore:
                     133.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.7e-129, bitscore:
                     418.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQDGRSMVTKNDYRFLHTLEDMRPATEPNLTVLYSSTLPENFKK
                     YAADISVTTSSIQYENDDVMRPVLGDDYSICCCVSATETGKEMQFFGARANLAKCLLYA
                     INGGVDEKTKQQVGPNYQPITSEYLDYDEVIAKYDKMMDWLAHLYVGTLNMIHYMHDKY
                     YYEAAEMALIDTKVERSFATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDF
                     PRYGNDDDRADEIATTLLSTFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDG
                     RKAGEPLAPGANPSYGAEQNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDER
                     SENLVHVLDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLT
                     REQQLDVIARTCHDRM"
ORIGIN
        1 agcgaaaact cacctccgca cagtctcaga aatccattct ttttcataca cggagcagca
       61 cacagcatgg catcctccaa atctctgttc ctgtcatatc cgctatcaga atcacccatt
      121 ccatcattct cggaccagac gtttttctgt tcattcgcag cgctgatcat aaacatcggc
      181 gtagaatgat aattcagctt ctcttcctga ctccacaccc gctgccagat cccggtatcc
      241 actgccacag attcaaaccc gttctccttc agaaacttcc tgtcccagga cggtctgttg
      301 atagaagaca gaggcatctg cagtgcaatc ttctccattc tgtcaatatc cgtacagagg
      361 taatgatcat ccagatgctc actctccaca ctctttcggt cttcctcata agacaggcgt
      421 ttctcctcat cataaagata accgtaccag tttgcatcga aattaagaag ctttccgccc
      481 ggtttcagta cacgacacca ctcttcataa gctctcttag gatccttcag attccatgtc
      541 agattcctgc tgatcacaac atcaaacaca tcatcctcaa attccagatt ctgagcatcc
      601 atcttataaa aagaaatccg ctcacaaaga ttaccggcat tcctcttcgc agtatccaac
      661 atctcctgtg tataatcaac agcagtcacc ttatatcccg cctcagccag aataacagga
      721 aaaaatccag gtcctgttcc gatatccaaa atcttaatct catccttcgc tttctccggg
      781 aactgccctt tcaacacctc aagccatgcc cccttctgca tcccattcaa ttccttatga
      841 ttaacttcag aatacccctc agcccgggta gtccagtaac tctcaatctc cgataataaa
      901 tccttcacct tatgccaacc tccatgcacg caatttataa tcatatatag ccaaaattat
      961 atcaaatccc tcccctttcc aacaagccct cccgggggat atatttcaca tctcaattca
     1021 tttttgcata aacaaagtcc ttcttaaaac tttttcacac ttcgccttgt atttatatat
     1081 taaccaaaac cagcgaagca ggcgtatagt tgtaccggat gcatttgcag gcatcttaga
     1141 gattcttagt cctcggaaat ctgcgttatg agacgaaaag tcccttgtct gagcgacagc
     1201 gagtaattcc ggacttttcg tctcatgctt ttagcggatt ttcgagggct tagaatctct
     1261 tagatgccga aaccgtatcc ggtacaacta tacgcctgct tcgcgtccct tttttaaaca
     1321 aataaagctg ccatacccca aaaagaggat atgacagcta tatattttta ctcagatagc
     1381 tccaagaatc ttccgggcat tatcaatcct ttcctgcgtc ggaggattaa ccccctccag
     1441 tggataagaa agccccagat tctcccattt aaacttacca agcgtatgat aagggagcac
     1501 ctccactctc tccacattgg aaagagtatg gataaaatca gctaaccggt gcagatattc
     1561 atccttatca cttccgtccg gcacaagcac atgacggatc catacaggtt ttttcatatc
     1621 agaaagttcc cgcgccatcg caagaatatt cttattggaa tgaccggtaa gcttaatatg
     1681 ctcctgctcg tcaatctgct tgatatcaag cattacaaga tctgtatact tcataagctc
     1741 cagccatccg gaatgccatg gttcctgatc agtgaaggga tttccactgg tatcaagatt
     1801 cgtatgcaca ccctctgcct ttgccttctt aaaaaattct gtaagaaaat ccatctgaag
     1861 caatggctca ccaccgctta cagtgatacc gcctttgctg ccccagtagc ccttataacg
     1921 taaagctttc ttaagaagct catctgccgt ataaagctct ccctgcttca tcttccaggt
     1981 atccggatta tgacagaact gacatctcat agcacagccg cttaaaaaaa tcacatatct
     2041 tactccgggt ccgtcaacgg acccgaagct ttctaaagaa tgaacatagc cttttatctg
     2101 tttattatcc attatatcat catccattaa ttacattctg tcgtggcagg ttctggcaat
     2161 aacgtctaac tgctgctctc ttgtaaggtc gatgaactta acagcatatc cggatacacg
     2221 gattgtgaag tttgcatact cttctttttc cggatgttcc atagcgtcga tcagtttctc
     2281 tttaccaaat acgtttacat tgaggtgatg agcaccctga tcaaagtatc cgtcaagtac
     2341 atgtaccaga ttctctgaac gctcgtcatc actgtgtccc agagcatcag gattgattgt
     2401 ctgagtattg gagataccgt caagagcatc ttcatagtca agcttggcaa cagagttcag
     2461 ggaagcaaga agaccgttct gctctgcacc gtaagatggg tttgcgccag gtgcaagagg
     2521 ttcccctgct tttcttccat ccggaagaga tccagtagcc ttaccataaa caacgttgga
     2581 agtaatagta aggatagatg tggttggctt ggaatcacgg taagtatgga tgtgtttcag
     2641 tttctcaagg aatgtagaca gaagagttgt tgcaatctcg tctgctctgt catcatcatt
     2701 accataacgt gggaagtcac cctcaacctg gaaatctgtt gtaataccgt cttcatcgcg
     2761 gatagcttta acttttgcat atttgatagc ggacagggag tctactacat gagagaatcc
     2821 tgcgatacct gttgcgaaag aacgctcaac ctttgtatcg atcagagcca tctctgctgc
     2881 ttcgtagtag tatttgtcat gcatgtagtg gatcatgttc agtgtaccga catacagatg
     2941 agccagccag tccatcattt tatcatattt tgcaattact tcatcatagt caagatattc
     3001 agaagtgatc ggctgatagt ttggtcctac ctgctgtttt gtcttctcat caacaccgcc
     3061 gttgatcgca taaagaaggc attttgcaag gtttgcacga gcgccgaaga actgcatctc
     3121 tttacctgtc tctgtagcag atacacagca gcagatgctg tagtcatcgc ccaataccgg
     3181 acgcataaca tcatcgttct catactggat ggaacttgtt gtaacagaga tgtctgctgc
     3241 atatttcttg aagttctcag gcagtgttga agagtagaga actgtaaggt tcggctccgt
     3301 agccggtctc atatcctcaa gtgtatgaag gaaacgatag tcgttctttg ttaccatgga
     3361 acgtccgtcc tgtcccatac
//
