LOCUS gnlProkkaGAGFMGDE_109 13781 bp DNA linear UNK 01-JAN-1980 DEFINITION gnl|Prokka|GAGFMGDE_109. ACCESSION gnlProkkaGAGFMGDE_109 VERSION gnlProkkaGAGFMGDE_109 KEYWORDS . SOURCE ORGANISM . COMMENT ##antiSMASH-Data-START## Version :: 7.1.0 Run date :: 2024-11-30 22:06:51 Original ID :: gnl|Prokka|GAGFMGDE_109 NOTE: This is a single region extracted from a larger record! Orig. start :: 0 Orig. end :: 13781 ##antiSMASH-Data-END## FEATURES Location/Qualifiers CDS 1..156 /locus_tag="ctg109_1" /transl_table=11 /translation="MEDCRKSKICVIIHSIKMKKNITEYLQNDKSLIIYRYVILLYYVK RELIFG" protocluster 1..13781 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="True" /core_location="[2192:3781]" /cutoff="20000" /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))" /neighbourhood="10000" /product="ranthipeptide" /protocluster_number="1" /tool="antismash" proto_core 2193..3781 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))" /neighbourhood="10000" /product="ranthipeptide" /protocluster_number="1" cand_cluster 1..13781 /candidate_cluster_number="1" /contig_edge="True" /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))" /kind="single" /product="ranthipeptide" /protoclusters="1" /tool="antismash" region 1..13781 /candidate_cluster_numbers="1" /contig_edge="True" /product="ranthipeptide" /region_number="1" /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))" /tool="antismash" CDS 214..1719 /locus_tag="ctg109_2" /transl_table=11 /translation="MSNNIRIRKKKLKGTGIFAAAALTAALIAGNNIPGVTRQVSAAES QENTVSSLIPDNITLDKPVELADVELPATEYGTFEWADGSFVPAQRVQSCEVLLIPAEG QDLSHVDGWDPESGAVVGYINVIVNSITDGDNVDKTEGSGKTDPTENADPTETPETSSE KPAADENADTDNKEDKESKSDQDNKTDTDNEESKIEEDKTDNKNNKDNNSNADKTDKDT ETTGKLEPADDMTQDKEDTKKDDEDGADDNIFDNPVLPDDKDDRPTDAEDSLSDEEKEE RAALNHTCDGITVSGVNLPWYVQFRVSSGDSYQFTNESDAMIFQSYEFELWDLKNNTEY EIPNGDYISVTVPVKSGYQYTIEHLLDNGATETIIPSVENGIMVFSTHSFSPFGIAGSR QLVGPDAGNDTDDTDAVTPAPASSDSQSSDSGKNNTVSMNDSSDSNNGSSSDQNDNSTE NKAVKNNNTVNTGDTTSIMPFVILFVAAAVIAGVVVFIKKRKK" CDS 1769..2119 /locus_tag="ctg109_3" /transl_table=11 /translation="MKVRAILKSLLFAYALTGVALLLLALVLFAFDLGETAVDAGIIII YVLACFMGGFMAGKIVRKDKYLWGVITGLAYYVLLLTVSIMAKGGWDMSAAHAVTTFFM CTGGGTLGGMLS" CDS 2193..2333 /gene_functions="biosynthetic (rule-based-clusters) ranthipeptide: TIGR03973" /gene_functions="biosynthetic (rule-based-clusters) ranthipeptide: SCIFF" /gene_kind="biosynthetic" /locus_tag="ctg109_4" /sec_met_domain="TIGR03973 (E-value: 1.1e-27, bitscore: 87.3, seeds: 29, tool: rule-based-clusters)" /sec_met_domain="SCIFF (E-value: 1.4e-27, bitscore: 87.0, seeds: 37, tool: rule-based-clusters)" /transl_table=11 /translation="MEHIKTLNTKNLQNTVKKGGCGECQTSCQSACKTSCTVGNQSCEQ K" CDS 2414..3781 /gene_functions="biosynthetic (rule-based-clusters) ranthipeptide: Ranthipeptide_rSAM_RRE" /gene_functions="biosynthetic (rule-based-clusters) ranthipeptide: TIGR04085" /gene_functions="biosynthetic (rule-based-clusters) ranthipeptide: PF04055" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_12" /gene_kind="biosynthetic" /locus_tag="ctg109_5" /sec_met_domain="Ranthipeptide_rSAM_RRE (E-value: 5.5e-29, bitscore: 92.0, seeds: 20, tool: rule-based-clusters)" /sec_met_domain="Fer4_12 (E-value: 3.8e-16, bitscore: 51.3, seeds: 84, tool: rule-based-clusters)" /sec_met_domain="PF04055 (E-value: 3.3e-14, bitscore: 45.3, seeds: 518, tool: rule-based-clusters)" /sec_met_domain="TIGR04085 (E-value: 8.3e-20, bitscore: 62.7, seeds: 281, tool: rule-based-clusters)" /transl_table=11 /translation="MSLIHRYKSNGFNIVLDINSGCIHLVDEVTYEVLPYLEEGLGTEA IAEKLENKYNREDIETSVRECNKLKEDGMLFTKDVYENVIEEFSNNRQTVVKALCLHIA HDCNLACRYCFAEEGEYHGRRALMSYEVGKKALDFLIANSGSRKNLEVDFFGGEPLMNW QVVKDLVKYGREQEKLHNKKFRFTLTTNGVLLNDEVMEFCNKEMGNVVLSVDGRKEVHD YMRPFRKGAGSYDLIMPKFQKFAESRNQDKYYVRGTFTHHNLDFSKDVLHLADLGFKQI SVEPVVAADTEEYAIREEDIPQIMEEYDTLAKEMIKREKEGKGFNFFHFMIDLTGGPCV YKRLSGCGSGTEYLAVTPWGDFYPCHQFVGEEQYLMGNVDEGITKPEIQKEFGGCNVYT KKDCKDCFARFYCSGGCAANAFHFQGDIKGNYEIGCELQRKRVECAIMIKAALACD" CDS 3893..6301 /locus_tag="ctg109_6" /transl_table=11 /translation="MKKSKGIVVLLLTLILTVFFCFTAAVGIGPTGTGAAKHINTGLDL AGGVSITYQAKESNPTSEEMSDTIYKLQKRVEQYSTEAQVYQEGSDRITVEIPGVTDAD TILNDLGKPGSLYFITKEDADGNQNFTTGQDGYVLSRTMDEIKESGSVVLEGSDVADAQ GGAIQNQNTSAKEYVVSLTLTSDGENSGKTKFAEATKNNVGKQIAIVYDNNIISAPNVN EEISGGKAQISGMSDLEEAQNLASYIRIGSLGLELEELRSSVVAAQLGEEAISTSVLAG AIGLIIVIIFMIIAYRVPGVVAGIALILYTSLMLITLNAFDITLTLPGIAGIILGIGMA VDANVIIYARIREEIGAGVSVRNSIKSGFSKAFSAIFDGNITTLIAAFVLMWLGSGTVK GFAYTLALGIVISMFTALVVSRLIVNALYAVGVRDPKFYGSAKERKAVDFLGKKKVFFA ISIILILCGPAAMFANSHAGNKALNYSLEFSGGTSTTVTFNEDMDIKTIDSEVTPVVEE VTGDKNVQPTKVVGTNQVVIKTRSLNQSEREALQSALVEKFGVDDSTISTESISSTVSS EMRRDAIVAVIVATICMLLYIWFRFKDIRFASSAVLALLHDVLVVLAFYAIARVSVGNT FIACMLTIVGYSINATIVIFDRIRENLHSGSREKLAEIVNTSITQTLTRSIYTSFTTFV MVAVLYIMGVSSIREFAAPLMVGVLVGAYSSVCITGALWYVMKKKFAGKGQPAIAAASA SAKSSSKPKKARDVSQKDPTQPKKKNRKRVAERLAAEEAAKKQQNDDAE" CDS 6383..8095 /locus_tag="ctg109_7" /transl_table=11 /translation="MEKWVIAAKRADFNQIGQQFHIDPVIARLIRNRDVIGEDKIREYL SGTVQELPSPWLMKDMEKAVDILEKKISQKAKVRIIGDYDIDGVTSTYILMKGLARIGA DVDTYIPDRVADGYGIHAHLIERAETDRIDTIVTCDNGIAAAAEIQMAKDKGMTVIITD HHEVPYREEKGERQMVLPPADAILNPKQYDCPYPNKNLCGAVVAFKYIAALYERFGVPA EELEDYYELAAIATVGDVMDLQGENRILVKEGLCRLKNTKNQGLQELIRANSLEDAKIT AYHIGFVLGPCINASGRLDTAARSLALLNAKTKEEAARLAGDLTALNQSRKALTEKGKE EAIRIIETTELKNDRVLVVYLPDCHESLAGIIAGRIREKYHRPAFVLTGGETSAKGSGR SIESYSMYEELVKCADLMIQFGGHPMAAGLSIEEKNIEEFRRRLNVNCTLTDEELRPKI VIDVPMPVSYITKELVEQISLLEPFGKGNTKPVFAQKNLRVLDHSIIGKNKNVVKLKLL DPQGISVEGIYFGEAEDFVNFIREKDIISVTYYPEINRFRGRESLQIIIQNYC" CDS 8243..8767 /locus_tag="ctg109_8" /transl_table=11 /translation="MKKIEDYVLSIPDFPEPGIIFRDITSVLQDADGLQLAIDSMQDCL KDIDVDVIAGTESRGFIFGVPIAYNLHKPFVPIRKKGKLPRETVSVSYDLEYGSAEIEM HKDSIKPGQKVVIVDDLIATGGTIEAAIKLVEQLGGEVVKVVFLMELAGLKGRERLNGY DVASVICYDGK" CDS 8805..11135 /locus_tag="ctg109_9" /transl_table=11 /translation="MTEITKSTEAKKDEMQVEKEKLESVKRADAAVKTMHDFTSPEVLY KELINSVLKYHPSTDISMIEKAYKVASEAHEGQKRKSGEPYIIHPLCVAIILADLELDK ETIVAGLLHDAVEDTWMTYEEVEKEFGSEVALLVDGVTKLGQLSYSADKVEVQAENLRK MFLAMAKDIRVILIKLADRLHNMRTLQYMRPEKQQEKARETMDIYAPIAMRLGISKIKV ELDDLSLKYLKPDVYYDLVHKVALRKSEREQFVGAIVKEVKKHMDDANIKAQVDGRVKH FFSIYKKMVNQDKTIDQIYDLFAVRILVDTVKDCYAALGVIHEMYKPIPGRFKDYIAMP KPNMYQSLHTTLIGPNGQPFEIQIRTYEMHRTAEYGIAAHWKYKESSDGKAPVGKSEEE KLNWLRQILEWQRDMSDNKEFMSLLKNDLDLFADSVYCFTPQGDVKTLPSGSTPVDFAY SVHSAVGNKMVGARVNGKLVPIEYEIKNGDRIEIITSQNSQGPSRDWLKLVKSTQAKNK INQWFKKELKEDNILKGKEMLAQYARAKGFKIANYTKTQYLEAVLRKYGFRDWDSVLAA IGHGGLKEGQVFNKLVEAYDKENKKNLTDEQVLEAASETQEKLHIAKSKSGIVVKGIHD VAVRFSKCCNPIPGDEIVGFVTRGRGITIHRTDCVNVLNMSETDRTRLIEAEWQQPDTK EKEKYMAEIQVYANNRTGLLVDLSKIFTERKIDLRSINSRTSKQEKATISMSFEIGSKE ELRSLIEKIRQVESVIDVERTTG" CDS 11200..11829 /gene_functions="biosynthetic-additional (smcogs) SMCOG1170:metallo-beta-lactamase family protein (Score: 163.1; E-value: 9.6e-50)" /gene_kind="biosynthetic-additional" /locus_tag="ctg109_10" /transl_table=11 /translation="MKNLELQKCILGPVYTNCYLLKNKETGELIIVDPADCPEKIEMKI SRMNGKPVAILLTHGHFDHILAAQAVKEKYNIPIYACRQEEEMLREPSINMTVHYGQGC SIVPDVFLEDLDVIRLAGFSVQMIHTPGHTKGSCCYYLKDEGVLFSGDTVFYGSVGRTD FPGGSTAEIVRSLHKLVDSLPEETEVFPGHDASTTIGYEKRYNPFV" CDS 11822..13336 /gene_functions="biosynthetic-additional (rule-based-clusters) PF04055" /gene_kind="biosynthetic-additional" /locus_tag="ctg109_11" /sec_met_domain="PF04055 (E-value: 1.9e-24, bitscore: 78.5, seeds: 518, tool: rule-based-clusters)" /transl_table=11 /translation="MCKIGILFKNRDFEHDVYELIKAFYPEAEIHTLYENGEAEYDLRF SVERDNDSYIIKYERTENLSKQETEQKGVISADILSKENAGCGIQDAHALRKENKDNIK YALYQLLVKLTGRTLPWGNLTGIRPAKLAMGMIESGMKNTEAAREMRERYLVSPQKTAL AITIANREREILKDIDYENGYSLYIGIPFCPSICLYCSFSSYPLKVWEKRTDEYVEALC REVRETALMMKGRKLDTIYIGGGTPTTLLPHQIRKLLDTVGEAFGYEGLAEFTVEAGRP DSITREKLMAIREYPVTRISVNPQTMNQETLDIIGRRHTVEQTKEAFHLARELGYDNIN MDLIVGLPGEDIHMVERTLEQVRELAPDSITVHSLAVKRAARLNIFKEKYQEMSFENNQ EIMDLTMKTAYEMGMGPYYLYRQKNMKGNFENVGYAKVDKAGIYNILIMEEKQPIIALG AGGSSKLVFDHGQRIERVENVKDVSNYISRIDEMIERKRTAIATWL" ORIGIN 1 aaagaggact gtcgaaaaag taagatttgt gttataattc actcgattaa aatgaaaaaa 61 aacattacag aatatttaca aaatgataaa agtttgataa tatacagata tgtaatatta 121 ttgtattacg taaaaagaga actgatattt ggctgaaagt aaaaaatgat tatcaggaaa 181 cataagaatc gaatataaat atagaggtgc gatatgtcca ataatataag aataagaaaa 241 aagaaattaa aaggaacggg tatttttgcg gcagcggctt taacagcggc tttgattgca 301 ggaaataata taccgggtgt aacaagacag gtcagtgcgg ctgagtcaca ggagaatact 361 gtgtcttctc tgattccgga caacatcacg cttgataagc cagtagaact tgcggatgta 421 gaacttccgg caacggaata tggaactttt gaatgggcag atggaagttt cgttcctgca 481 cagcgtgtac agagctgtga ggtacttctt ataccggcag aagggcagga tttaagccat 541 gtggatggat gggatccgga gagcggcgcg gttgtcggtt acatcaatgt aatagtgaac 601 agtattacgg atggtgataa tgttgataag acagaaggct caggtaaaac agatccaaca 661 gaaaatgcag atccaacaga aacgcctgaa acttcgtcag aaaaacctgc cgcagatgaa 721 aacgctgata cagacaataa agaagataag gaaagtaaat cagatcagga taataaaact 781 gatacagaca atgaagagag taagattgaa gaagataaaa cagataataa aaacaacaaa 841 gataataaca gcaatgcaga taagacggat aaagatacag aaactaccgg taagctggaa 901 cctgcagatg atatgacaca ggataaagaa gatacgaaga aagatgatga agatggcgca 961 gatgataata tctttgataa tcctgttctg ccggacgata aggatgaccg tcctacagat 1021 gcagaggatt ctctttctga tgaagaaaaa gaagagaggg ccgcacttaa tcatacctgc 1081 gacggtatta cagtaagcgg agttaatctt ccatggtatg tacagttcag agtttcaagc 1141 ggtgacagct atcagtttac caatgaatca gatgcaatga ttttccagtc ttatgaattt 1201 gaactctggg atcttaagaa taatacagaa tatgagatcc ctaatggaga ttatatcagt 1261 gtcactgtgc cggtaaaatc cggatatcag tatactatcg aacatctcct ggacaacggg 1321 gcaactgaga cgattattcc gagtgtagag aatggtatta tggtattcag cacacattct 1381 ttcagtccat ttggaattgc aggaagcaga caattagtag gacctgatgc ggggaatgat 1441 acggacgaca cagatgcggt aactcctgca ccggcttcct ccgacagtca gagctctgat 1501 tccggaaaga acaatacagt atctatgaat gacagttctg acagcaataa tggttcttct 1561 tctgatcaga atgataattc aacggaaaat aaagctgtga aaaataacaa tacagtaaat 1621 accggagata caacatcaat tatgccgttt gtgatactgt ttgtggcagc tgcagtgatc 1681 gccggtgtgg ttgtatttat aaaaaagaga aagaaataaa agaataacac tgattcctcc 1741 cccatatagt ttaacaggag gggaagcaat gaaagtcaga gcaattttga aatcactatt 1801 atttgcatat gcattaacag gagtggcatt actgcttttg gccctggtac tgtttgcttt 1861 tgatctggga gaaacagctg tagatgcggg tattattata atttatgtac ttgcatgttt 1921 catgggaggc tttatggccg gcaaaattgt gcgtaaagac aaatatctgt ggggtgttat 1981 cacaggtctg gcttactatg tattactgtt gactgtctct attatggcaa aaggcggctg 2041 ggatatgagt gcagcgcatg cagttacaac attttttatg tgcacaggcg gtggaacact 2101 gggtggaatg ctgtcgtaaa aaaaagcttt taaaatgaaa agatatatga tatactatgc 2161 caagaatata attacataag gaggatagag ttatggaaca tatcaaaacc ctgaatacaa 2221 agaatttaca gaacactgta aaaaaaggtg gatgtggcga gtgccagact tcctgccagt 2281 ccgcatgtaa aacttcctgt acagtaggaa accagagctg cgagcagaaa taagatcagc 2341 tatggagaaa tgagataagc agcggaactt ttattccgct gcttatctta cgcttagaaa 2401 ggattttagt aaaatgagtc tgattcaccg ttataaaagc aatggcttta atattgtact 2461 ggatatcaac agtggatgca tccatctggt agatgaggtt acatatgagg ttcttccata 2521 tctggaagag ggccttggca cagaagctat tgcagagaaa ctggaaaaca aatataacag 2581 agaagatatt gagacatctg taagagaatg taataagctg aaagaagatg gaatgctttt 2641 tacaaaagat gtttatgaga atgtaattga agaattttca aataaccggc agaccgttgt 2701 aaaagcattg tgcctgcaca ttgctcatga ctgcaatctg gcctgccgtt attgttttgc 2761 cgaagaaggc gaatatcatg gcagaagagc tcttatgtcc tatgaagtcg gcaaaaaagc 2821 tcttgatttc ctgatcgcga actccggttc cagaaaaaac ctggaagtag atttctttgg 2881 cggcgagccg ctgatgaact ggcaggttgt aaaagatctg gtaaaatatg gcagagagca 2941 ggaaaagctt cacaataaga aattccgttt cactttaaca acaaacggag ttcttctgaa 3001 tgatgaagta atggaattct gcaataaaga gatgggcaac gttgtactga gtgtggacgg 3061 acgtaaggaa gtacatgatt acatgcgtcc attcagaaaa ggtgcaggaa gctatgacct 3121 gatcatgccg aagttccaga agtttgcaga atccagaaat caggacaagt attatgtaag 3181 aggtactttt acccaccata atcttgattt ctccaaggat gtgcttcatc tggcagatct 3241 tggattcaaa cagatttctg tagagcctgt agttgcggct gatacggagg aatatgcgat 3301 ccgcgaggag gatatccccc agatcatgga agagtatgat actcttgcaa aagaaatgat 3361 taaaagagag aaagagggaa aaggatttaa cttcttccat tttatgatcg accttacagg 3421 tggcccttgt gtatacaaga gactgtcagg ctgcggttca ggaacagagt acctggctgt 3481 aacaccctgg ggtgattttt acccatgtca ccagtttgtt ggtgaagaac agtatctcat 3541 gggaaatgta gacgaaggca tcacaaagcc ggaaatccag aaggaattcg gcggatgcaa 3601 cgtttacaca aagaaagact gcaaagactg cttcgcacgt ttctactgta gtggcggatg 3661 tgcggcaaat gcattccatt tccagggaga tatcaaaggc aattatgaaa tcggatgcga 3721 actccagaga aaacgtgtgg agtgcgccat catgataaaa gccgcactgg catgtgatta 3781 gtgggcagta atctgtctga ctgataccgc cgtcggctgt tatcataaca gaaaatggct 3841 ggcaggcgga tgaacatgac cgcggacaaa aaatcaagaa gggaaaagaa ctatgaagaa 3901 aagtaaagga atcgtagtgc tgcttctgac actgatcctg actgtgttct tttgctttac 3961 tgcagccgta ggtatcggtc ctaccggaac cggtgcagca aaacatatca acacgggtct 4021 tgatcttgcc ggtggtgtca gcattaccta ccaggctaag gagagcaatc cgacaagcga 4081 agagatgtca gatacgatct acaagcttca gaagcgtgta gagcagtaca gtactgaggc 4141 tcaggtatac caggaaggat cagaccgtat tacagttgaa attcctggtg ttacagatgc 4201 agatactatt ttgaatgatc tgggtaaacc aggttccctg tatttcatca caaaagaaga 4261 tgctgacgga aaccagaact ttactacagg acaggatgga tatgttctct ccagaactat 4321 ggatgagatc aaggaatccg gaagtgttgt tctggaaggt tctgatgtag cagatgccca 4381 gggtggtgcg atccagaatc agaacacaag tgcaaaggaa tatgtggtaa gtcttactct 4441 tacttctgat ggagaaaaca gtggtaaaac aaaatttgca gaagctacaa agaataatgt 4501 aggcaagcag atcgctatcg tctatgacaa taacattatc agtgctccga atgtaaatga 4561 agagatttcc ggcggaaaag cacagatcag cggaatgtct gacctggaag aagcacagaa 4621 ccttgcttct tatatccgta tcggttctct gggactggaa ctggaagaac ttcgttccag 4681 tgttgtagct gcacagcttg gtgaagaagc aatctctaca agtgttcttg caggtgcgat 4741 cggtctgatc atcgttatta tctttatgat cattgcatac cgcgtacctg gtgtagttgc 4801 aggtatcgca ctgattcttt atacatcatt aatgctgatc acgctgaatg cctttgatat 4861 tactcttact cttccgggta ttgcgggtat tattcttggt atcggtatgg cggtggatgc 4921 caatgttatt atttatgccc gaatcagaga ggaaattggt gcaggagttt cagtaaggaa 4981 ttctattaag tccggtttca gcaaagcttt ctctgcaatc tttgatggaa atattacaac 5041 actgatcgca gcctttgtgc tgatgtggct tggttcaggt actgttaagg gatttgctta 5101 tacacttgcc cttggtatcg tgatctccat gtttacagca ctggttgtat ccagactgat 5161 tgtaaatgca ctttatgcag ttggtgtacg tgatccgaaa ttctacggat ctgcaaaaga 5221 acgtaaggca gttgatttcc ttggaaagaa gaaagtattt tttgctattt ccattatcct 5281 gatcctctgc ggtccggcag cgatgtttgc taattcgcat gcaggaaaca aagcgttaaa 5341 ttacagcctt gagttctcag gcggtacttc tacgactgtt acatttaatg aagatatgga 5401 tattaagacc attgattctg aagtaactcc tgttgtagaa gaggtgacag gagataaaaa 5461 tgttcagcct acaaaggtag tcggaacaaa tcaggttgtg atcaagacac gttctctgaa 5521 tcagagcgaa cgtgaagctc tgcagagtgc actggttgag aaatttggtg tagatgacag 5581 cacgatttcc acagaaagta tttcttctac agtaagtagt gaaatgcgtc gtgatgcgat 5641 cgtagccgtt atagtagcta caatctgtat gcttctttat atctggttca gatttaaaga 5701 catccgtttt gcaagcagtg cggtattggc gcttctgcat gatgttctgg ttgtacttgc 5761 attctatgct attgccagag tatctgtcgg taatacattt attgcctgca tgcttacaat 5821 cgtaggttat tctatcaatg ctacaatcgt tatttttgac cgtatacgtg agaaccttca 5881 cagtggttcc agagaaaaac tggcagaaat tgtaaacaca agtatcactc agactcttac 5941 aagaagtatt tatacctctt ttacaacttt tgttatggta gctgttcttt atatcatggg 6001 tgtttcttct atccgtgaat ttgcagcacc tctgatggtt ggtgttcttg tcggtgcata 6061 ttcttctgta tgtattacag gtgcgttgtg gtatgtgatg aaaaagaaat ttgcaggaaa 6121 aggacagccg gcaattgctg cagcttccgc atctgcaaag tcctcttcaa agccgaagaa 6181 agcacgtgat gtatcacaga aagatccgac tcagccaaag aagaaaaaca gaaaaagagt 6241 agctgaacgt ctggctgccg aagaagcagc taaaaaacag cagaatgatg atgctgaata 6301 aaacaataat tgtctgaaaa acaggcagac agaaatatcc cggggtaaaa ctccgggata 6361 tttttatgag gagagaaaat atatggagaa atgggtaata gcagcaaaaa gagcagattt 6421 taaccagatc ggtcaacagt ttcatattga tccggtgatt gcgagactga tcaggaacag 6481 agatgtgatc ggagaagata agataagaga gtatctgtca ggaacagtac aagagcttcc 6541 ttctccctgg ctgatgaagg atatggaaaa agctgtagat atcctggaga aaaaaatatc 6601 gcagaaagca aaagtccgta ttattggtga ttatgatatt gatggtgtga cctctacata 6661 cattttaatg aaaggccttg cgaggatcgg agcagatgtg gatacttata ttccggacag 6721 agttgcagac ggatatggga ttcatgcaca tcttatagaa agagcggaaa cggaccggat 6781 cgatacgatc gtgacctgtg ataatggaat tgctgcggct gctgaaatcc agatggcaaa 6841 ggataaggga atgacagtga ttattacaga tcatcatgag gttccttaca gggaggaaaa 6901 gggagaacgc cagatggtgc ttccgccggc agatgcgatt ttgaatccga aacaatatga 6961 ttgtccatac ccgaataaaa acctgtgtgg ggcagtggtg gcctttaaat atattgcagc 7021 actttacgag agatttggtg ttccggcaga ggagctggaa gattattatg aactggcggc 7081 tattgcaaca gtaggagatg ttatggatct tcagggagag aacaggattc tggtaaaaga 7141 agggctgtgc cgtctgaaaa ataccaaaaa tcaaggattg caggaactga tccgagcaaa 7201 ttctctggaa gatgctaaaa tcacagctta tcatatcgga tttgtgctgg gaccatgtat 7261 caatgccagt ggcagactgg atacagctgc ccgttctctt gcacttctca atgcaaaaac 7321 aaaggaagaa gcagcaaggc ttgccggaga tcttacagct cttaatcaga gccgaaaagc 7381 tctcactgag aaaggaaaag aagaggcaat ccggattata gagactacag agctgaaaaa 7441 tgacagagta ctggtagttt atcttccgga ttgtcatgaa agtcttgccg gtattatagc 7501 aggcaggatc agggaaaaat atcacaggcc ggcctttgta ttgacgggcg gtgagacaag 7561 tgcaaaagga agcggaagat caatagaaag ttactctatg tatgaagaac tggtaaaatg 7621 tgcagacctg atgatacagt ttggaggtca tccgatggca gcaggtctgt ctatagaaga 7681 aaaaaatata gaagaattcc ggagacgcct caatgtcaac tgtacgttga cggacgaaga 7741 attgcgtcct aagatcgtga ttgatgtgcc gatgcctgtg tcttatatta caaaagaact 7801 ggtagaacag atttcgcttc tggaaccctt tggaaaaggg aatacaaaac cggtctttgc 7861 gcagaaaaat ctcagagtgc ttgatcacag tattatcggg aaaaataaaa atgtagtaaa 7921 actaaaactt ctcgatccgc aggggatttc agtggaaggc atttatttcg gagaagcaga 7981 ggattttgtg aactttatca gggaaaaaga cattatttct gttacttatt atccggagat 8041 caacagattc aggggacgag agagtcttca gattattatt cagaattact gttaatatta 8101 ccgcttggtt tgtaaccgcc ggtaagcctg gaaatacaaa tgtcagggct taacagtaac 8161 ttgattctga aataaaaagg tgatatacta taaaatattt atgagcgaac agcaatataa 8221 cacacactgg aggagaaata aaatgaagaa aattgaagat tatgtattaa gtataccgga 8281 ttttcctgaa ccggggatta ttttcagaga tattacaagt gttctgcagg acgcggatgg 8341 cctgcagttg gcaatcgact ccatgcagga ctgtcttaag gatatagatg tggacgtgat 8401 tgcaggaaca gagtccagag gctttatctt tggtgtaccg atcgcatata atcttcataa 8461 accatttgta ccgattcgta aaaaaggtaa acttccccgc gaaacagtat ctgtaagcta 8521 tgatctggaa tatggcagtg cagagattga aatgcataaa gattccataa aaccaggtca 8581 gaaagtggtt atcgttgatg atctgatcgc aacaggcggt acgatcgaag cagcgatcaa 8641 gctggtggaa cagcttggcg gtgaggttgt caaggttgta tttctcatgg aacttgcagg 8701 tttaaaggga cgcgaaagac tgaatgggta tgatgtagcg tctgtaattt gttatgatgg 8761 taaataagca cagctgattg tgcaggacag gcaggtggga gcatatgaca gagataacaa 8821 aaagtacaga agcaaaaaaa gatgaaatgc aggttgaaaa agagaagctg gaatctgtaa 8881 agagagcaga tgcggcagta aagaccatgc atgattttac aagtccggag gtgctttata 8941 aagaactgat aaacagtgtt ctgaagtatc atccatcaac agatatttcc atgatagaga 9001 aggcatataa ggttgcaagt gaagctcatg aaggacagaa gagaaagtct ggagagccat 9061 atatcattca tcctttgtgt gttgccatca ttctggcaga tctggaactt gacaaagaaa 9121 cgatagttgc aggtcttctt catgatgcag ttgaagatac ctggatgacc tatgaggaag 9181 ttgagaagga atttggttca gaggttgcac ttcttgtgga tggtgttacc aagctgggac 9241 agttatctta ttctgcggat aaagtagaag ttcaggcaga gaacctcaga aagatgtttc 9301 ttgccatggc taaggatatc agagttattc tgatcaagct tgcagaccgt cttcataata 9361 tgcgtacctt acagtatatg cgtcctgaaa agcagcagga aaaggccaga gagaccatgg 9421 atatttatgc tcctattgcc atgcgtctgg gtatttccaa gataaaggta gagctggatg 9481 acctgtcgct gaaatacctt aagccggatg tatattatga cctggtgcat aaggttgccc 9541 tgcgtaaaag cgagagagaa cagtttgtag gtgcgattgt aaaagaagtt aagaaacata 9601 tggatgatgc caatattaag gcgcaggtgg acggacgtgt aaaacacttt ttcagcatct 9661 acaagaagat ggtaaatcag gataagacta ttgatcagat ttatgattta tttgctgtcc 9721 gtattcttgt agatacagtg aaggattgtt atgctgccct tggtgtgatc catgagatgt 9781 ataagccaat tccgggacgg tttaaagatt atattgccat gccgaaaccc aatatgtatc 9841 agtcattgca taccacattg atcggaccca acggacagcc ctttgaaatc cagatccgta 9901 cttatgaaat gcacagaact gcagaatatg gtatcgctgc acactggaaa tataaggagt 9961 cgtctgacgg aaaggctcct gtaggtaaga gtgaggaaga gaaactgaac tggctgcgtc 10021 agattctgga atggcagaga gacatgtctg ataacaaaga attcatgagt cttctgaaga 10081 atgacctgga tctttttgcg gacagtgttt actgtttcac tccacaggga gatgttaaaa 10141 cacttccaag cggttctaca ccggtggatt ttgcgtacag tgttcacagt gcagtcggaa 10201 ataagatggt aggtgccaga gttaacggta agctggttcc gattgaatat gagatcaaaa 10261 acggcgaccg tattgaaatc attacttcac agaattctca gggaccaagc cgcgactggt 10321 tgaaactggt taagagtact caggcaaaga acaagattaa ccagtggttc aagaaggaat 10381 taaaagaaga taatatcctt aagggcaagg agatgcttgc gcagtatgcg cgtgcaaagg 10441 gatttaagat cgcaaattat acaaaaaccc agtatctgga ggctgttttg cgtaaatatg 10501 gtttcaggga ctgggattca gtacttgctg caattggtca tggcggattg aaagaaggtc 10561 aggttttcaa taagctggta gaggcttatg ataaggaaaa taagaagaat ctgacagatg 10621 agcaggttct tgaggctgct tctgagacac aggagaagct tcacattgcc aagagcaaga 10681 gtggaattgt ggtaaaagga atccatgatg tggcagtccg tttttccaag tgctgtaatc 10741 cgatcccggg tgacgagatt gtaggctttg ttacaagagg acgtggaatc acaatccatc 10801 gtacagattg cgtcaatgtg ctgaatatgt ctgagacgga caggacacga ttgatcgagg 10861 cggaatggca gcagcctgat accaaggaaa aagagaagta tatggctgag attcaggttt 10921 atgcaaataa ccgtacaggg cttcttgtgg atctttctaa aatatttaca gaacgtaaga 10981 tcgacctgcg aagtatcaac agccgtacaa gcaagcagga gaaggcaact atttccatga 11041 gttttgagat tggaagcaaa gaggaactgc gttccctgat cgagaagatc cgtcaggtag 11101 agagtgtcat tgacgtagag agaactacag gctgatcatg gaaaaatctg ctggaacatt 11161 attttatgga actgctgcag attgacagaa aggaataaca tgaagaattt agaattacag 11221 aaatgtatat taggacctgt atatacaaat tgctatctcc ttaagaataa agaaaccgga 11281 gaactgatca ttgttgatcc cgcagattgt ccggagaaga ttgaaatgaa gatttcccgg 11341 atgaatggaa aaccggtggc gattcttctt acccatggac attttgacca cattctggct 11401 gcacaggcag taaaggagaa atataatatt ccgatttatg cctgtcgtca ggaagaggag 11461 atgctccgcg aaccttcgat caatatgact gtacattatg gacagggatg ttctattgtg 11521 ccggatgtat ttctggagga tctggatgtt atcagactgg cgggattttc cgttcagatg 11581 atccatacac cgggacatac caaaggaagc tgctgttatt atctgaaaga tgaaggtgta 11641 ttgttcagcg gtgatacagt gttctatggc tctgttggaa gaacggattt tccgggtgga 11701 agtacagcag aaattgtgag aagtctccat aaactggtag attcgcttcc tgaagagacc 11761 gaggttttcc ccggacatga tgcatctacc actattggtt atgaaaagag gtataatcca 11821 tttgtgtaaa ataggcattc tgtttaagaa cagagatttt gaacatgatg tttatgaact 11881 gatcaaagcg ttttatcccg aagcggagat acatactctt tatgaaaatg gtgaagcaga 11941 gtatgacctc cgcttcagtg tggaacggga taatgacagt tatatcataa aatacgaaag 12001 gacagagaat ctgtcgaaac aggaaacaga gcaaaaaggg gtaatttccg cagacatact 12061 ttctaaagag aatgcaggct gtggaataca ggatgctcat gctctgagaa aagagaataa 12121 agataatatc aaatatgctc tgtatcagtt gttggtaaaa ctgacgggca gaactcttcc 12181 atggggaaat ctgacaggaa tacgtcctgc caaactggca atgggaatga tcgaatccgg 12241 aatgaaaaat acggaagcag cccgggagat gcgtgagaga tatctggtga gtccgcagaa 12301 aacagcgctt gccatcacta ttgcaaacag ggaaagagag attctgaagg atattgacta 12361 tgaaaatgga tacagccttt atattggaat cccgttttgc ccaagtatct gtctgtattg 12421 ttccttcagc tcctatccgc tgaaagtgtg ggagaagaga acagatgaat atgtggaagc 12481 tctctgccgt gaggtaagag agactgctct tatgatgaag ggaagaaaac tggacaccat 12541 ctatattggc ggcggtaccc cgactacact gcttccacat cagatccgta agctcctgga 12601 tacagtggga gaagcttttg gctatgaagg actggcagag tttactgtgg aagccggcag 12661 accggacagt attacacgtg aaaagctgat ggcgatcagg gagtatccgg taacaagaat 12721 ttccgtaaat ccacagacta tgaatcagga aaccctggat attatcggcc gcagacatac 12781 tgtggagcag acaaaagaag cgtttcatct ggcacgagag ctgggatacg ataacattaa 12841 catggatctg atcgtaggac tcccgggtga ggatattcac atggtagagc ggactcttga 12901 acaggttcgt gaactggcac cggacagtat taccgtacat tcactggcag tgaaacgtgc 12961 agcaaggctg aatattttta aagagaaata tcaggaaatg tcttttgaga ataatcagga 13021 gattatggat ctgaccatga aaactgcgta tgaaatggga atgggtcctt attatctgta 13081 cagacagaag aatatgaagg gaaattttga aaatgttggc tatgcaaagg ttgacaaagc 13141 gggaatttac aatatactga ttatggagga aaaacagcct atcatcgcac tgggggcagg 13201 cggttcttcc aagctggtct ttgaccatgg acagagaatt gaacgtgtgg agaatgtaaa 13261 agacgtttct aattatattt cccgcattga tgagatgatc gagagaaaac ggacagcaat 13321 agctacctgg ctgtaggatg tggaggtaac attttggaca cagtacaaaa gacagcagca 13381 ggttcacagc agaggatcct ggaatttgac cttgcttctg aactgcatca tggaatgctg 13441 gtaagtaatc tggcttatgc ggttgcagag gagatgggac tgccgcatga acagtgttat 13501 gagcttgcaa tagcaggaat gctccatgat ataggaaagc tgaagctgac aagttatatt 13561 aacggacagg agcaggatcc tctggtgatc gaggaactga agtatgtccg gatgcattct 13621 gcattgggat atgaggaact taaagggcag ggatattcgg attttgtatt ggagagtatc 13681 ctgtaccatc atgagaatta tgatggtagt ggatatccgt ccaataaagc aggtgatgat 13741 atacccatag gtgcccggat actcagagtc tgcgatgtat a //