GCA_008672595.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaGAGFMGDE_62 (original name was: gnl|Prokka|GAGFMGDE_62)

Region	Type	From	To	Most similar known cluster		Similarity
Region 62.1	lanthipeptide-class-ii	1	4,385

gnlProkkaGAGFMGDE_97 (original name was: gnl|Prokka|GAGFMGDE_97)

Region	Type	From	To	Most similar known cluster		Similarity
Region 97.1	cyclic-lactone-autoinducer	1	15,841

gnlProkkaGAGFMGDE_109 (original name was: gnl|Prokka|GAGFMGDE_109)

Region	Type	From	To	Most similar known cluster		Similarity
Region 109.1	ranthipeptide	1	13,781

gnlProkkaGAGFMGDE_160 (original name was: gnl|Prokka|GAGFMGDE_160)

Region	Type	From	To	Most similar known cluster		Similarity
Region 160.1	NRPS	20,501	56,592	dipeptide aldehydes	NRP	47%

Region	Type	From	To	Most similar known cluster	Similarity
Region 62.1	lanthipeptide-class-ii	1	4,385
Region 97.1	cyclic-lactone-autoinducer	1	15,841
Region 109.1	ranthipeptide	1	13,781
Region 160.1	NRPS	20,501	56,592	dipeptide aldehydes	NRP	47%

Compact view

gnlProkkaGAGFMGDE_62 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 4,385 nt. (total: 4,385 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg62_2

ctg62_1

Leader:

MEKELDEIEIIDTESIEEKLSFIVYG

Core:

TIVCC

Core with dehydrations:

DhbIVCysCys

ctg62_5

Leader:

MRFAKLKLRTLYQVEFANSKKYKFQEEMNMVGT

Core:

MSEKKFICLWIYNGK

Core with dehydrations:

MDhaEKKFICysLWIYNGK

antiSMASH-DB 4.0 matches for ctg62_5

100.0% NZ_JADPGO010000016.1and 1 other:

NZ_JAQDGJ010000020.1 Lanthipeptides: allorf_03808_03954_lanthipeptide

Lanthipeptides: allorf_03938_04084_lanthipeptide

1

MSEKKFICLWIYNGK

15

MSEKKFICLWIYNGK

1

MSEKKFICLWIYNGK

15

7.5% NZ_CP093302.1: Lanthipeptides: allorf_0563784_0563987_lanthipeptide

7

ICLWIY

12

ICLW++

47

ICLWVF

52

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg62_1 - Class II: Cleavage pHMM score: -5.90; RODEO score: 0; Monoisotopic mass: 519.2 Da; Molecular weight: 519.7 Da; Number of bridges: 1
ctg62_5 - Class II: Cleavage pHMM score: -3.70; RODEO score: 0; Monoisotopic mass: 1840.9 Da; Molecular weight: 1842.2 Da; Number of bridges: 1

gnlProkkaGAGFMGDE_97 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 15,841 nt. (total: 15,841 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGAGFMGDE_109 - Region 1 - ranthipeptide

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Location: 1 - 13,781 nt. (total: 13,781 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGAGFMGDE_160 - Region 1 - NRPS

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Location: 20,501 - 56,592 nt. (total: 36,092 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 20500 - 56592:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg160_45

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)