LOCUS       c00385_gnlPro..         3460 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EKGOBLFL_385.
ACCESSION   c00385_gnlPro..
VERSION     c00385_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 22:26:55
            Original ID  :: gnl|Prokka|EKGOBLFL_385
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3460
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3460
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2794]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2794
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..3460
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3460
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..358
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg385_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.7e-26, bitscore:
                     80.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GGLQMNLQELGTIMEQKAPVKMILLNNNFLGNVRQWQAMFFNRRY
                     SFTPMMNPDYMKIASAYDIPARRVMTREELAKAIDEMIATDGPFLLEACVEEEGNVMPM
                     TPPGGSVNQMLLEC"
     CDS             391..951
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg385_2"
                     /sec_met_domain="ALS_ss_C (E-value: 3e-20, bitscore: 60.8,
                     seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKLYTIIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVTKQITKKIDVLQAHYFTDDEIYQHEIALYKITTPTLEEKPE
                     VSKVIRKYNARIVEVNAVFAIVEKNGMSEEITNLYEELRQLDCVLQFVRSGRVAITTSC
                     FERVNEYLADREAKYRQSKHQES"
     CDS             948..1700
                     /locus_tag="ctg385_3"
                     /transl_table=11
                     /translation="MMSENNKIGTYKFVAEPFHVDFTGRLTMGVLGNHLLNCAGFHASD
                     RGFGIATLNEDNYTWVLSRLAVELDEMPYQYEDFSIQTWVENVYRLFTDRNFAIIDKEG
                     KKIGYARSVWAMISMNTRKPADLLALHSGSIVDYVCDEPCPIEKPSRIKVASKEPVAAL
                     VAKYSDIDINGHVNSIRYIEHILDLFPIEMYKEKRIRRFEMAYVAESYYGDELTLFMDD
                     AGEGVYDVEVKKNGSEVVCRSKVMFTEK"
     CDS             1751..2794
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg385_4"
                     /sec_met_domain="IlvN (E-value: 1.9e-47, bitscore: 149.3,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1.7e-41, bitscore: 130.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGVIENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQ
                     ACNLRDNGFNVIVGQRPGKTYEKAVADGWVPGETLFGIEEACQKGTIIMCLLSDAAVMS
                     VWPTIKPYLTPGKALYFSHGFAITWNDRTGVVPPKDIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAVYQDATGKAFDKTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMT
                     PFHDAIKPVVQKLYASVKSGNEAQISIDSNSKPDYREKLNAELKALRESEMWQTAVTVR
                     KLRPENN"
     CDS             complement(2856..3458)
                     /locus_tag="ctg385_5"
                     /transl_table=11
                     /translation="VQYRMHEEIMKFPSQWFYNGELEAASEVRYRGILDWDTPIHWIDT
                     SEMDFKEEFVGETFGRINKAEADLLLSELKIYINRISGNRILEEKIDFGIISPYKAQVQ
                     YLRNKIKADASLKPYRSLFTVNTVDGFQGQERDVIFISLVRANEEGQIGFLNDLRRMNV
                     AITRARMKLVILGEAETLKHHGFYRKLLEFIQNIGNQ"
ORIGIN
        1 cggcggcttg cagatgaacc tgcaggaact gggtaccatc atggaacaaa aggctccggt
       61 gaagatgatt ctgttgaaca ataacttcct gggcaatgtt cgtcaatggc aggcgatgtt
      121 cttcaatcgt cgttattcgt ttactccgat gatgaatccg gattatatga agattgcttc
      181 ggcatatgat attcctgcac gcagggtaat gacccgcgag gagctggcaa aagcgattga
      241 cgagatgata gcgactgacg gaccattcct gctcgaagct tgtgtggagg aagaaggaaa
      301 tgtaatgccg atgacacctc cgggcggttc ggtcaatcag atgttgttgg aatgctgaag
      361 tcatgcgaaa ttaaagaatt tactctatct atggataaaa aactatatac aatcattgtt
      421 cattcggaaa actttgccgg tttgctgaat caggtgacgg ctgtgtttac ccgccggcag
      481 ataaacatcg agagcttgaa tgtatcggcc tcctccatca agggggtaca taaatatacc
      541 attaccgctt ggacggataa ggataccatt gaaaaggtga ccaagcagat taccaagaaa
      601 atagatgtgc tgcaagccca ttatttcacg gatgatgaaa tctatcagca cgagattgcc
      661 ctttataaaa taacgacccc tacgctggaa gaaaaaccgg aggtgtccaa agtaatccgt
      721 aagtacaatg cccgtattgt ggaggtgaat gctgtatttg ccattgttga gaagaatggc
      781 atgagtgagg aaataaccaa tctttatgag gagttgcgcc agttggattg cgtacttcag
      841 ttcgtccgtt cgggtcgcgt agccattacg accagctgct ttgagcgtgt caatgaatat
      901 ctggcggacc gtgaagcaaa atatcgtcaa agtaaacacc aggaatcatg atgagcgaga
      961 acaataaaat aggtacctac aagtttgttg ccgaaccgtt tcatgtcgac tttaccggac
     1021 ggctgacaat gggagtattg ggcaaccatt tgttgaattg tgcgggtttc catgccagcg
     1081 accgcggttt tggcatcgcg actctaaacg aggacaatta tacttgggta ttgtcccgtc
     1141 tggctgtcga actggatgaa atgccttatc agtacgaaga tttctccatt cagacttggg
     1201 tggagaatgt ctatcgcctt tttacagacc gtaatttcgc tatcatcgat aaggagggca
     1261 agaagattgg gtatgcacgt tccgtttggg cgatgattag catgaatacc cgtaagccgg
     1321 cagatttgct ggctcttcat agtggcagca ttgtagatta tgtttgcgat gagccttgcc
     1381 cgatagaaaa accttcgcgg ataaaggtgg cctcaaagga accggttgct gctttggtag
     1441 ccaaatacag tgatattgac attaacgggc atgtgaacag catccgttac attgagcata
     1501 tccttgattt gttcccgata gagatgtata aggagaagcg catccgtcgt tttgaaatgg
     1561 catatgtagc cgaaagctat tatggggatg agttgacttt gttcatggat gatgccggtg
     1621 agggcgttta cgatgtggag gtgaaaaaga atggcagcga agtggtctgt cgctctaaag
     1681 tgatgtttac agagaaataa ataaatttat caacgttggc gtaagccaca aataaaaatt
     1741 aaaacaaaaa atggcacaat tgaattttgg cggtgttatt gagaatgtaa tgacccgcga
     1801 agaatttcct ttggaaaaag cacgtgagat tttgaaagat gaaacaattg cagtaatcgg
     1861 ttatggcgta caaggcccgg gccaggcttg caacttgcgt gataacggtt tcaatgtgat
     1921 tgtaggacaa cgtccgggca agacatacga aaaggctgtg gctgacggat gggtaccggg
     1981 tgagactttg ttcggcatcg aagaggcttg tcagaaaggt actatcatca tgtgtctgtt
     2041 gtctgacgca gctgtaatgt ctgtatggcc gactatcaaa ccttatttga ctcccggaaa
     2101 ggctctttac ttctctcatg gttttgctat tacctggaac gaccgtaccg gcgtggttcc
     2161 tcctaaagat attgatgtta ttatggttgc tcccaaaggt tccggtactt cacttcgtac
     2221 catgttcctc gagggtcgtg gcttgaactc atcatatgcc gtctatcagg atgctacggg
     2281 taaggctttt gacaagacga ttgcactggg tatcggcatc ggttcgggat atttgttcga
     2341 gacaactttc atccgtgaag ccacttccga cttgaccggt gaacgtggtt ccttgatggg
     2401 tgctatccag ggattactgt tggctcagta tgaggtattg cgcgaaaacg gccatactcc
     2461 ttctgaagca ttcaatgaaa cagtggaaga gttgactcaa tctttgatgc cattgtttgc
     2521 caagaatggt atggactgga tgtatgccaa ctgttctact acggcacagc gtggtgcgct
     2581 ggattggatg accccgttcc atgatgccat caagccggtt gtacagaaat tgtatgcaag
     2641 tgtgaagtcg ggtaacgaag ctcagatttc tatcgacagt aactccaaac ccgattatcg
     2701 tgagaagctg aatgcagaat tgaaagcctt gcgtgaaagc gaaatgtggc agacagctgt
     2761 tacagttcgt aaacttcgtc cggaaaacaa ttaatcagct ttatataaat aggtgaaata
     2821 ccctcttcgg tgttaccgga gggggtattt ctttattatt gattccctat attttgaata
     2881 aactccaata gcttccggta aaaaccgtga tgtttcagcg tctctgcctc tcccaatatc
     2941 accagcttca tacgtgcccg ggtgatggct acattcattc tccttaaatc attcaggaat
     3001 cctatctgcc cttcttcgtt ggcacggaca aggctgatga agataacatc gcgctcttgc
     3061 ccttggaagc catccaccgt atttacagtg aacaagctgc gataaggctt gagtgaagcg
     3121 tctgccttta tcttgttgcg gagatattgc acttgtgcct tgtaagggga gatgatgccg
     3181 aagtctattt tttcttccag aatgcggttg ccactgatac ggttgatgta gattttcagt
     3241 tctgatagga gcaggtccgc ttccgcctta ttaatgcgcc cgaaagtctc tcccacaaat
     3301 tcttctttga aatccatttc ggaggtgtca atccagtgga tgggggtatc ccagtccagg
     3361 atgccccggt aacgcacttc ggaagcggct tccaactcgc cattataaaa ccattgggaa
     3421 ggaaatttca tgatttcctc gtgcatgcgg tattgtactt
//
