LOCUS       c00476_gnlPro..         4779 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DGLEAMLD_476.
ACCESSION   c00476_gnlPro..
VERSION     c00476_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 22:58:10
            Original ID  :: gnl|Prokka|DGLEAMLD_476
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4779
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4779
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[602:3558]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      603..3558
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4779
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4779
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             603..2831
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg476_1"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1169.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2833..3558
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg476_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.5e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(3627..4778)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 85.9; E-value: 5e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg476_3"
                     /sec_met_domain="Fn3-like (E-value: 2.4e-28, bitscore:
                     85.9, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 4.8e-54,
                     bitscore: 171.1, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGLFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRES
                     VLPLTAQTRKIALIGGLSKSQYDMAGAWAATTDRNKVVTLYDALVRKGLEVSYAEGYDM
                     KSNQLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGK
                     PVIALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAH
                     VGQIPIYYQNKSTGRPTALKQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKA
                     AGVHTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNVGESKTVAF
                     QITDDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 cacaacggtt ttcaagaccg ccgcaatcga ccactctgcc acctctccaa aacttctttt
       61 tcagaagtgc ttttctttta aagcggtgca aagatacaaa tcatttttta ttctacaaac
      121 ttttctgcac aaaaacagag caattatacc aaaattcttg tttataccca ctacggaacg
      181 aacataacgg catttaggct atttgttgaa atttatcaag ggcaagacag agttttataa
      241 cttactggct atcagtatat ttacaaagag ccacattcag tatacgattg aaccctattc
      301 agtatacgac tgaatggcat tcactatacg actgaattag gttcagaata cgactgttcc
      361 ttaaacctac atcatttgta aataatgcag attattattc ggttcttgtt tcaattcata
      421 aaccacatat aaagaaagta ttaacatctc tactcccccg gacattcact acctaataat
      481 agttatttta tgctgttcaa cataaaaaga gaagtatctc ttataaaatt acggaattta
      541 ttcagtattt ttgcgcgctc taaaggataa cattatctaa cctactaaca aaaacaaatt
      601 ttatggaatt gaacaaaacc tttaccaatg ggctttggaa taaagaaatc aatgtcagtg
      661 atttcgtcag taaaaacatc gcaccctata ccggggatgc atctttcttg cagggaccta
      721 ccgaacgtac caaacgtatc tgggacatct gtctgaaggc tctcgaagaa gaaaggaaca
      781 acaatggcgt ccgttcactg gacaatacca ccgtatctac catcacttct cataaagccg
      841 ggtatattga taaggacaat gaacttattg tcggtttgca gactgatgaa ctgttgaaac
      901 gatccatcaa acctttcgga ggcatcaacg tggtagcaaa agcatgccgt gaaaacggag
      961 tggaagtgga cgataaagta aaagacatct tcacccatta ccgcaaaaca cataacgacg
     1021 gcgtattcga tgtatatacc gaagaaatcc gttccttccg ctcactggga ttccttaccg
     1081 gattgcccga caactatgcc cgcggacgta tcatcggcga ctaccgccga ttggctctct
     1141 acggcatcga ccgtctgatt gaagccaagc aggaggactt gcgcaacctc accggcccga
     1201 tgaccgaagc acgcatccgt ctacgcgaag aagtggcaga acaaatcaaa gctttgaagg
     1261 acatcaaggt tatgggcgaa tattacggac tcgacctcag ccatccggct acatcagctc
     1321 aagaggccgt gcaatgggta tacatggcat atcttgctgc catcaaagag caggacggag
     1381 cagccatgtc actgggtaac gtatcttcgt tcctcgatat attcattgaa tacgatctgg
     1441 ctcacggaaa aatcaatgag acgtttgcac aggaactgat agaccagttc gtcatcaagc
     1501 tgcgcatggt acgccatctg cgtatgcaat catacaatga tatctttgcc ggtgacccga
     1561 cttgggtgac cgaagccatc ggaggacgct tcaatgacgg acgcgtgaag gtcaccaaga
     1621 cttctttccg ctttttgcag acgctataca acctcggtcc ttctccggaa ccgaacttga
     1681 ccgtattgtg gagtcccgaa ctgccggaag gcttcaagga tttctgcgcc aaagtttctg
     1741 tagataccag ctccatccag tacgaaaacg acaacctgat gcgtgaagtg cgcaactgcg
     1801 acgactacgg catcgcctgc tgtgtatcct accaagccat cggcaaacaa atccagttct
     1861 tcggcgcacg tgccaacctt gccaaggccc tgctgctcgc catcaacggt ggacgttgcg
     1921 aaaataccgg aactgtcatg gtgaaaggta ttcccgtatt gaccggtgag acattgaagt
     1981 ttgaggaagt tatggccaac tacaagaagg tactgactga aatagcccgt gtctacaacg
     2041 aggccatgaa tatcatccac tacatgcatg ataagtatta ttatgagaaa gcccagatgg
     2101 cattcatcga taccgaccca cgcatcaatc tggcatacgg tgttgccggc ctttccatcg
     2161 ccatagactc actgtccgcc atcaaatatg ccaaagtcac tgcacgccgc aacgaaatcg
     2221 gcctgaccga aggtttcgac actgaaggtt cattcccctg cttcggcaat aatgacgacc
     2281 gtgtggacca cctgggagta gacctcgttt actatttcag cgaagaactg aaaaagctgc
     2341 ctgtctacaa gaatgcccgt cccacccttt ccttgctgac tatcacctcc aacgtgatgt
     2401 acggcaagaa gaccggtgcc actccggacg gacgcgccaa aggtgttgcc tttgctccgg
     2461 gtgccaaccc aatgcacgga cgtgacaaga acggtgccat cgcttccttg agctccgtag
     2521 ccaaattgcg ctaccgcgac tcgcaggacg gcatcagcaa tacgttctcc attgttccca
     2581 aatcactggg cccgacaccg gaagaacgtg tagagaacct ggtaaccatg atggacggct
     2641 acttcaccaa aggtgcccac catttgaacg taaacgtact gaaccgcgaa atgctggaag
     2701 atgccatgga acatcctgaa aagtatccgc aactcaccat ccgcgtttcc ggctatgccg
     2761 tgaacttcgt gaagttgagc cgcgagcatc agttggaagt catcagccgt tcattccacg
     2821 aaagaatgta atatgataaa tgtacactcc tacgaaagta tgggaacatt cgacgggccg
     2881 ggtctccggc tcgtcgtttt cctgcaagga tgtccgttcc gttgtctgta ttgtgccaat
     2941 ccggatacga ttgatgtaaa aggaggcact cccaccactc cggaagagat tctgcaaatg
     3001 gctatcagcc aaaaggcatt cttcgggaag aaaggaggta ttactttctc cggcggtgag
     3061 cctaccctgc aagcagaagc gctgattcct cttttcaagg agctgaaggc gaacggcatc
     3121 catatctgtc tggacagtaa cggaggcatc tggaatgaaa aggtggaaga gctctttagc
     3181 ctgactgacc tcgtgctgct ggacatcaag caattcaatc ccgaacgcca ccgtacgctg
     3241 acgggacgaa gcaacgaaca gacactccgc accgctgcct ggctcgaaga acatgaacac
     3301 cccttctggc tgcgctatgt actggtcccc ggttacagcg attcggaaga agacacccgc
     3361 ctgctgggtg aaagtcttgg caaataccag cagatacaac gggtggaaat acttccttat
     3421 caccgtctgg gtgttcacaa atacgaagcc atgggatggg attacaaatt gaaagaagtg
     3481 aaggaaaata ctccggaaca actggaacgg gcagaaaaac tgttcaaaga gtatttcacg
     3541 aatgtgataa ccaactaaaa cagaaaagca aggctgtccg gatatgattc cagacagcct
     3601 tgcttttcat ttatagggta agaatcctat tctttcaagt tataggtaat ctgctgtaca
     3661 tcatcactac tgccacccac cataaagacg aaatcaccag gttctaccat ataattcatc
     3721 tcattgtcat agaatcccag tgcatcgtca gttatctgaa aagctacagt tttgctttca
     3781 cccacattca gatgaatttt cctgaagccc ttcaattcct ttatcggacg cgtaacagag
     3841 gctaccttat cctgcacata caattgaact acttcctctc ctgcacactt accggtatta
     3901 gtcactttta ccgacacctc cgtatggacg cctgcagcct tgcccggcag cagtttcaaa
     3961 tcagaatatc caaaatccgt gtacgaaagc ccatatccga aaggataggc aggctcagtg
     4021 gaagcatcga tataagatgc cttataggtc tgtttcaatg cggtagggcg gccggtgctc
     4081 ttattctgat aataaatggg aatctgtcct acgtgtgcag gaaatgtcat gggcaattta
     4141 gccgaaggat tatactctcc ccacaacaca ttgcatatgg cattgccggc ctcacttccc
     4201 agccaccaag tacacagtac cgcatcagca tgttccctta tctcattgaa gattaccgga
     4261 cgcccacaca tgacaagagc aatgacaggt tttccagtct ttgccagttc cgacaccagc
     4321 ttctgctgcc cctcgggtat ggctatattc actttggaag ccttctcgcc actctgaccg
     4381 aatctctcgc ctaccgccac aacaatgaca tccgaagcct cagcagcctg taatgcttcc
     4441 tgcaatccgg tcaactgatt gctcttcata tcatagcctt cagcatagct cacctccagt
     4501 cctttccgga ccaatgcatc atataaggtc accactttgt tccggtccgt agtggcagcc
     4561 caagcacctg ccatatcgta ctgggatttt gagagtcctc ctatcaaggc tatcttcctc
     4621 gtctgtgccg tcaacggaag cacagattcc ctattctcca gcaatacaac ggaacgctcg
     4681 gccatacgga gcgaagcatt acggacttcg ggcgcattga gtattctggc cgctctttcc
     4741 ggattacagt agcgaaaagg gtcctcaaac aatcccaac
//
