LOCUS       c00216_gnlPro..         5181 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CJDNIIFN_216.
ACCESSION   c00216_gnlPro..
VERSION     c00216_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 22:57:45
            Original ID  :: gnl|Prokka|CJDNIIFN_216
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5181
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5181
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1581:5181]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1582..5181
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5181
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5181
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             3..1520
                     /locus_tag="ctg216_1"
                     /transl_table=11
                     /translation="MEVQETDNLGVQLYFDETSYRTMFEALEDTLRSKGNRLAELRDIL
                     LGTSKAGFRELYPVRFPWLNSTQETAVNKVLCARDVAIVHGPPGTGKTTTLVEAIYETL
                     HREPQVLVCAQSNTAVDWISEKLVDRGVNVLRIGNPTRVNDKMLSFTYERRFENHPLYP
                     ELWSIRKNLRELGSRARRGSYDEREGVRSRMSRLRDRATALEIQINSELFDGAHVIAST
                     LVSSNHRLLNGRRFGTLFIDEAAQALEAACWIAIRKADRVVLAGDHCQLPPTIKCYEAA
                     RGGLERTLMEKVVSNKPAVVSLLKVQYRMHEEIMKFPSQWFYNGELEAAPEVRYRGILD
                     WDTPIHWIDTSEMDFKEEFVGETFGRINKAEADLLLSELKIYINRISGNRILEEKIDFG
                     IISPYKAQVQYLRNKIKADASLKPYRSLFTVNTVDGFQGQERDVIFISLVRANEEGQIG
                     FLNDLRRMNVAITRARMKLVIMGEAETLKHHGFYRKLLEFIQNIGNQ"
     CDS             complement(1582..2625)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_2"
                     /sec_met_domain="IlvN (E-value: 2e-47, bitscore: 149.3,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1.7e-41, bitscore: 130.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGVIENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQ
                     ACNLRDNGFNVIVGQRPGKTYEKAVADGWVPGETLFGIEEACQKGTIVMCLLSDAAVMS
                     VWPTIKPYLTPGKALYFSHGFAITWNDRTGVVPPKDIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAVYQDATGKAFDKTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMT
                     PFHDAIKPVVQKLYASVKSGNEAQISIDSNSKPDYREKLNAELKALRESEMWQTAVTVR
                     KLRPENN"
     CDS             complement(2676..3428)
                     /locus_tag="ctg216_3"
                     /transl_table=11
                     /translation="MMSENNKIGTYKFVAEPFHVDFTGRLTMGVLGNHLLNCAGFHASD
                     RGFGIATLNEDNYTWVLSRLAVELDEMPYQYEDFSIQTWVENVYRLFTDRNFAIIDKEG
                     KKIGYARSVWAMISMNTRKPADLLALHSGSIVDYVCDEPCPIEKPSRIKVASKEPVAAL
                     VAKYSDIDINGHVNSIRYIEHILDLFPIEMYKEKRIRRFEMAYVAESYYGDELTLFMDD
                     AGEGVYDVEVKKNGSEVVCRSKVIFTEK"
     CDS             complement(3434..3985)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_4"
                     /sec_met_domain="ALS_ss_C (E-value: 2.9e-20, bitscore:
                     60.8, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKLYTIIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVTKQITKKIDVLQAHYFTDDEIYQHEIALYKITTPTLEEKPE
                     VSKVIRKYNARIVEVNAVFAIVEKNGMSEEITNLYEELRQLDCVLQFVRSGRVAITTSC
                     FERVNEYLADREAKYRQSKH"
     CDS             complement(4018..5181)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 6.6e-47, bitscore:
                     147.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KVDYVRSYLPVPEMEPEAIRKAAELINGAERPLVLVGQGVELGEA
                     QQELRAFIEKAGMPAGCTLLGLSALPTNHPLNKGMLGMHGNLGPNINTNKCDVLIAVGM
                     RFDDRVTGKLETYAKQAKIIHFDIDPAEIDKNVKTDVAVLGDCKETLAAVTQLLEPANH
                     QEWIDSFRQYEEVEEEKVIRPELHPAGKALSMGEVVRAVSEATHNEAILVTDVGQNQMM
                     SARYFKYSKERSIVTSGGLGTMGFGLPAAICATFGRPDRTVCVFMGDGGLQMNLQELGT
                     IMEQKAPVKMILLNNNFLGNVRQWQAMFFNRRYSFTPMMNPDYMKIASAYDIPARRVMT
                     REELAKAIDEMIATDGPFLLEACVEEEGNVMPMTPPGGSVNQMLLEC"
ORIGIN
        1 ttatggaagt gcaggaaacg gacaacctcg gcgtgcaact ttatttcgac gaaacatcct
       61 atcggaccat gttcgaagcc ttggaagata cgttacgctc caaaggcaac cgcctggccg
      121 aattgcggga catattgctg ggaacctcga aagccggttt ccgagagctc taccccgtac
      181 ggtttccctg gctgaactcc acgcaggaga cagctgtaaa caaagtgctg tgtgcccgcg
      241 acgtggccat tgtacatggt cctccgggaa ccggaaaaac gaccacctta gtggaagcca
      301 tctacgaaac cctgcatcgg gaaccgcagg tactggtatg tgcccaaagc aataccgccg
      361 tcgactggat ttcggaaaaa cttgtggacc gtggcgtcaa tgtcctgcgt atcggcaatc
      421 cgacccgtgt caacgacaag atgctttcct ttacctacga gcgtcgtttc gagaaccatc
      481 cgctttatcc ggaactgtgg agcatccgga aaaatctccg tgaactgggc agccgtgccc
      541 gccgtggcag ctatgacgaa cgcgaaggag tacgcagccg tatgagccgc ctgcgcgacc
      601 gcgccaccgc attggaaata caaatcaatt cagagctttt tgatggggca catgtcatag
      661 cctccacgct ggtaagcagc aatcaccggc tgctgaacgg acgccgtttc ggcacccttt
      721 tcattgatga agcagcccag gccttagagg ctgcctgctg gatagctatc cgaaaagcag
      781 accgcgtggt attggcgggt gaccactgcc aacttccacc caccataaaa tgctacgaag
      841 ccgcccgtgg aggtctggaa cgaacattga tggagaaagt ggtgtccaac aaacctgccg
      901 ttgtctcttt gctgaaagtg caataccgca tgcacgagga aatcatgaaa tttccttccc
      961 aatggtttta taatggcgag ttggaagccg ctcccgaagt gcgttaccgg ggcatcctgg
     1021 actgggatac ccccatccac tggattgaca cctccgaaat ggatttcaaa gaagaatttg
     1081 tgggagagac tttcgggcgc attaataagg cggaagcgga cctgctccta tcagaactga
     1141 aaatctacat caaccgtatc agtggcaacc gcattctgga agaaaaaata gacttcggca
     1201 tcatctcccc ttacaaggca caagtgcaat atctccgcaa caagataaag gcagacgctt
     1261 cactcaagcc ttatcgcagc ttgttcactg taaatacggt ggatggcttc caagggcaag
     1321 agcgcgatgt tatcttcatc agccttgtcc gtgccaacga agaagggcag ataggattcc
     1381 tgaatgattt aaggagaatg aatgtagcca tcacccgggc acgtatgaag ctggtgataa
     1441 tgggagaggc agagacgctg aaacatcacg gtttttaccg gaagctactg gagtttattc
     1501 aaaatatagg gaatcaataa taaagaaata ccccctccgg taacaccgaa gagggtattt
     1561 cacctattta tataaagctg attaattgtt ttccggacga agtttacgaa ctgtaacagc
     1621 tgtctgccac atttcgcttt cacgcaaggc tttcaattct gcattcagct tctcacgata
     1681 atcgggtttg gagttactgt cgatagaaat ctgagcttcg ttgcccgact tcacacttgc
     1741 atacaatttc tgtacaaccg gcttgatggc atcatgaaac ggggtcatcc aatccagcgc
     1801 accacgctgt gccgtagtag aacagttggc atacatccag tccataccgt tcttggcaaa
     1861 caatggcatc aaagattgag tcaactcttc cactgtttca ttgaatgctt cagaaggagt
     1921 atggccgttt tcgcgcaata cttcatactg agccaacagc aatccctgga tagcacccat
     1981 caatgaaccg cgttcaccgg tcaagtcgga agtggcttcg cggatgaaag ttgtctcgaa
     2041 caaatatccc gaaccaatac cgatacccag tgcaatcgtc ttgtcaaaag ccttacccgt
     2101 agcatcctga tagacggcat atgatgagtt caagccacgc ccttcgagga acatggtacg
     2161 aagtgaagta ccggaacctt tgggagcaac cataataaca tcaatatctt taggaggaac
     2221 cacgccggta cggtcgttcc aggtaatagc aaaaccatga gagaagtaaa gagcctttcc
     2281 gggagtcaaa taaggtttga tagtcggcca tacagacatt acagctgcgt cagacaacag
     2341 acacatgacg atagtacctt tctggcaagc ctcttcgatg ccgaacaaag tctcacccgg
     2401 tacccatccg tcagccaccg ccttttcgta tgtcttaccc ggacgttgtc ctacaatcac
     2461 attgaaaccg ttatcacgca agttgcaagc ctggcccggg ccttgtacgc cataaccgat
     2521 tactgcaatt gtttcatctt tcaaaatctc acgtgctttt tccaaaggaa attcttcgcg
     2581 ggtcattaca ttctcaataa caccgccaaa attcaattgt gccatttttt gttttaattt
     2641 ttatttgtgg cttacgccaa cgttgataaa tttatttatt tctctgtaaa tatcacttta
     2701 gagcgacaga ccacttcgct gccattcttt ttcacctcca catcgtaaac gccctcaccg
     2761 gcatcatcca tgaacaaagt caactcatcc ccataatagc tttcggctac atatgccatt
     2821 tcaaaacgac ggatgcgctt ctccttatac atctctatcg ggaacaaatc aaggatatgc
     2881 tcgatgtaac ggatgctgtt cacatgcccg ttaatgtcaa tatcactgta tttggctacc
     2941 aaagcagcaa ccggttcctt tgaggccacc tttatccgcg aaggtttttc tatcgggcaa
     3001 ggctcatcgc aaacataatc tacaatgctg ccactatgaa gagccagcaa atctgccggc
     3061 ttacgggtat tcatgctaat catcgcccaa acggaacgtg catacccaat cttcttgccc
     3121 tccttatcga tgatagcgaa attacggtct gtaaaaaggc gatagacatt ctccacccaa
     3181 gtctgaatgg agaaatcttc gtactgataa ggcatttcat ccagttcgac agccagacgg
     3241 gacaataccc aagtataatt gtcctcgttt agagtcgcga tgccaaaacc gcggtcgctg
     3301 gcatggaaac ccgcacaatt caacaaatgg ttgcccaata ctcccattgt cagccgtccg
     3361 gtaaagtcga catgaaacgg ttcggcaaca aacttgtagg tacctatctt attgttctcg
     3421 ctcatcatga ttcctagtgt ttactttgac gatattttgc ttcacggtcc gccagatatt
     3481 cattgacacg ctcaaagcag ctggtcgtaa tggctacgcg acccgaacgg acgaactgaa
     3541 gtacgcaatc caactggcgc aactcctcat aaagattggt gatttcctca ctcatgccat
     3601 tcttctcaac aatggcaaat acagcattca cctccacaat acgggcattg tacttacgga
     3661 ttactttaga cacctccggt ttttcttcca gcgtaggagt cgttatttta tagagggcaa
     3721 tctcgtgctg atagatttca tcatccgtga aataatgggc ttgcagcaca tctattttct
     3781 tggtaatctg cttggtcacc ttttcaatgg tatccttgtc cgtccaagcg gtaatggtat
     3841 atttatgtac ccccttgatg gaggaggccg atacattcaa actctcgatg tttatctgcc
     3901 ggcgggtaaa cacagccgtc acctgattca gcaaaccggc aaagttttcc gaatgaacaa
     3961 tgattgtata tagtttttta tccatagata gagtaaattc tttaatttcg catgacttca
     4021 gcattccaac aacatctgat tgaccgaacc gcccggaggt gtcatcggca ttacatttcc
     4081 ttcttcctcc acacaagctt cgagcaggaa cggtccgtca gtcgctatca tctcgtcaat
     4141 cgcttttgcc agctcctcgc gggtcattac cctgcgtgca ggaatatcat atgccgaagc
     4201 aatcttcata taatccggat tcatcatcgg agtaaacgaa taacgacgat tgaagaacat
     4261 cgcctgccat tgacgaacat tgcccaggaa gttattgttc aacagaatca tcttcaccgg
     4321 agctttttgc tccatgatgg tacccagttc ctgcaggttc atctgcaagc cgccgtctcc
     4381 catgaatacg cataccgtgc ggtccggacg tccaaaagtg gcacagatag ctgccggaag
     4441 gccgaagccc atagtaccca ggccaccgga agtaacaatg ctacgttcct tgctgtattt
     4501 gaaataacgg gcggacatca tctgattctg gccgacatca gtcaccaata tcgcctcgtt
     4561 atgggtagcc tcactcacag cacgcaccac ctcacccatg cttagcgctt ttcctgccgg
     4621 atgtaattcc ggacggatta ccttttcctc ttccacttcc tcgtattgcc ggaaactgtc
     4681 aatccattcc tgatggttgg cgggttccag caattgggta acggcagcca gtgtctcttt
     4741 gcaatctccc aggacagcaa catcggtctt tacattctta tctatttcgg cagggtcaat
     4801 atcaaagtgg ataatcttcg cctgcttggc atatgtttcc aatttacccg tcacacggtc
     4861 atcaaagcgc atgcctacgg cgataaggac atcacattta ttggtattga tattgggccc
     4921 caggttaccg tgcataccaa gcattccttt gttcaacgga tggttcgtag gtaatgcgga
     4981 aagtcccaat aaagtgcatc cggcaggcat tccggctttc tcaatgaaag cacgaagctc
     5041 ttgctgggct tctcccagct ccacgccttg tcctaccagc accaaaggcc gttccgcacc
     5101 atttattaat tcagcagcct tccgtatggc ctccggctcc atttcgggaa cgggaagata
     5161 gctacggaca tagtccacct t
//
