LOCUS       gnlProkkaACIBFKND_548   5357 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ACIBFKND_548.
ACCESSION   gnlProkkaACIBFKND_548
VERSION     gnlProkkaACIBFKND_548
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 04:48:11
            Original ID  :: gnl|Prokka|ACIBFKND_548
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5357
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..174)
                     /locus_tag="ctg548_1"
                     /transl_table=11
                     /translation="MNEGGVILYPTDTVWGIGCDATNEEAVRRVYEIKKRADSKAMLVL
                     VDSPVKVDFYVQD"
     protocluster    1..5357
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3389:4613]"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3390..4613
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
     cand_cluster    1..5357
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APE_KS1 or APE_KS2)"
                     /kind="single"
                     /product="arylpolyene"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5357
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="arylpolyene"
                     /region_number="1"
                     /rules="(APE_KS1 or APE_KS2)"
                     /tool="antismash"
     CDS             522..953
                     /locus_tag="ctg548_2"
                     /transl_table=11
                     /translation="MEEIVFHHTLPIQLRFNDVDKFGHVNNTVYFSFYDLGKTEYFASV
                     CPGVDWEKIGIVVVHIEADFVKQIFASDHIAVQTAVSKIGTKSFHLIQRVIDTETNEVK
                     CICKSVMVTFDLERHESMPLTEEWIEAICKYEERDLQKA"
     CDS             1110..2633
                     /locus_tag="ctg548_3"
                     /transl_table=11
                     /translation="MIADKSINLDTLYKVLFDNEKLELSKECIRKVEESFDFLQSFSSD
                     KIIYGINTGFGPMAQYRIEDQSLIELQYNIIRSHSTGAGKPLPELYVKAAMIARLYTFL
                     QGKSGVHMELVSLLCEFINRGIYPFIPEHGSVGASGDLVQLAHIALTLIGEGEVFYQGK
                     LRDAATVLEENGLQPFSMRIREGLSVTNGTSVMTGIGIVNLIYAKKLLRWSVAASVMMN
                     EIAASYDDFMAQALNEAKHHKGQQEIAAMMREWVAGSKCVLQRENELYNQVHKEKIFEH
                     KVQPYYSLRCVPQILGPIYDELKNAEEVLINEINSACDNPIVDPDTQNIYHGGNFHGDY
                     ISFEMDKLKIAVTKLTMLCERQINYLFHDRINGILPPFVNLGVLGLNYGLQASQFTATS
                     TTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALLAKTVIENSYQVMAIQFMGMAQAI
                     DYLKIQDRLSPKSRQVYEEIRSFFPVFTNDTPKYKEIERMMDYLKKENK"
     CDS             2635..3363
                     /NRPS_PKS="Domain: PKS_KR (3-169). E-value: 1.3e-16. Score:
                     52.9. Matches aSDomain: nrpspksdomains_ctg548_4_PKS_KR.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     213.3; E-value: 6.4e-65)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg548_4"
                     /sec_met_domain="adh_short (E-value: 1.9e-36, bitscore:
                     114.6, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYALVTGGSRGIGRAVSCKLAEMGYFILINYQSNDAEAEKTLQL
                     VQEKGSNGEIMKFDVTDPAAIALALNNWASQHPDEYIEVLINNAGIRKDNLMLWMTGEE
                     WNKVLDISLNGFFYVTQPLLKNMLVKRYGRIVNIVSLSGIQGMPGQANYSAAKGGVIAA
                     TKALAQEVAKKKVTVNAVAPGFIRTDMTEGIDENEWKKHIPAGRFGTAEEVADLVGFLA
                     SPASSYITGEVISINGGLYT"
     aSDomain        2644..3141
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg548_4_PKS_KR.1"
                     /evalue="1.30E-16"
                     /label="ctg548_4_PKS_KR.1"
                     /locus_tag="ctg548_4"
                     /protein_end="169"
                     /protein_start="3"
                     /score="52.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="ALVTGGSRGIGRAVSCKLAEMGYFILINYQSNDAEAEKTLQLVQE
                     KGSNGEIMKFDVTDPAAIALALNNWASQHPDEYIEVLINNAGIRKDNLMLWMTGEEWNK
                     VLDISLNGFFYVTQPLLKNMLVKRYGRIVNIVSLSGIQGMPGQANYSAAKGGVIAATKA
                     LAQ"
     CDS_motif       2647..2709
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg548_4_0001"
                     /evalue="2.90E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg548_4"
                     /protein_end="25"
                     /protein_start="4"
                     /score="13.5"
                     /tool="antismash"
                     /translation="LVTGGSRGIGRAVSCKLAEMG"
     CDS             3390..4613
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (65-405). E-value:
                     2.2e-53. Score: 173.5. Matches aSDomain:
                     nrpspksdomains_ctg548_5_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 295.4; E-value:
                     1.2e-89)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg548_5"
                     /sec_met_domain="APE_KS1 (E-value: 9.7e-161, bitscore:
                     523.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVVITGMGIYSCIGKNLDEVKDSLYNGKSGIGIDPVRKELGYF
                     SALTGILERPDLKKLLDRRKRLCLPEQGEYAYLATLEAFRNAGIDDAFLEANEVGILYG
                     NDSSAAPVINAVDIIREKKNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTIAGACA
                     SGSHAIGMGYLLIKSGLQDCILCGGAQEVNPYAVGSFDGLSAFSTQEAEPEKASKPFDK
                     RRDGLIPSGGAASLVLESYESAVKRGAPILAEVIGYGFSSNGDHISVPNVDGPKRSLQM
                     AIKDAGIALEQISYINAHATSTPVGDLNEAKAIAEVFEGHHPYVTSTKSMTGHEMWMAG
                     ASEVIYSILMMNNGFIAPNLNFEEADEASAQLNIPTQRVNLEFDTFLSNSFGFGGTNST
                     LIIRQIKE"
     aSDomain        3585..4604
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg548_5_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.20E-53"
                     /label="ctg548_5_PKS_KS.1"
                     /locus_tag="ctg548_5"
                     /protein_end="405"
                     /protein_start="65"
                     /score="173.5"
                     /tool="antismash"
                     /translation="RLCLPEQGEYAYLATLEAFRNAGIDDAFLEANEVGILYGNDSSAA
                     PVINAVDIIREKKNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTIAGACASGSHAI
                     GMGYLLIKSGLQDCILCGGAQEVNPYAVGSFDGLSAFSTQEAEPEKASKPFDKRRDGLI
                     PSGGAASLVLESYESAVKRGAPILAEVIGYGFSSNGDHISVPNVDGPKRSLQMAIKDAG
                     IALEQISYINAHATSTPVGDLNEAKAIAEVFEGHHPYVTSTKSMTGHEMWMAGASEVIY
                     SILMMNNGFIAPNLNFEEADEASAQLNIPTQRVNLEFDTFLSNSFGFGGTNSTLIIRQI
                     "
     CDS             4635..4886
                     /NRPS_PKS="Domain: PP-binding (7-75). E-value: 3.8e-14.
                     Score: 44.9. Matches aSDomain:
                     nrpspksdomains_ctg548_6_PP-binding.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg548_6"
                     /sec_met_domain="PP-binding (E-value: 4.7e-15, bitscore:
                     44.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNEEIIEKIRTTLAEEFEVDIDVIQPDAPLMETLELDSLDLVDM
                     VVLVEKNFGFNVTGQDFAGIKTFQDFYDLVITRMQEAK"
     aSDomain        4656..4859
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg548_6_PP-binding.1"
                     /evalue="3.80E-14"
                     /label="ctg548_6_PP-binding.1"
                     /locus_tag="ctg548_6"
                     /protein_end="75"
                     /protein_start="7"
                     /score="44.9"
                     /tool="antismash"
                     /translation="EKIRTTLAEEFEVDIDVIQPDAPLMETLELDSLDLVDMVVLVEKN
                     FGFNVTGQDFAGIKTFQDFYDLV"
     CDS             4889..5356
                     /locus_tag="ctg548_7"
                     /transl_table=11
                     /translation="MSTWKGKTRGGTFGYLFFIYLIKYLGITAAYLFLSLVVLYFIPFA
                     PKATKSTWFYARHILKYNRIRSLKMLLLNYYRLGQILIDKVAIGNGKVNQYQFKFEQYP
                     EFLQLLNSEQGVIMIGAHVGNWEIGVPFFDDYGKKINIVMYDAEHRRIKEIL"
ORIGIN
        1 atcctgtaca taaaaatcca ctttaaccgg agaatccacc aacaccaaca tagctttgct
       61 atcagcacgt ttcttgatct catatacccg gcgtacagcc tcttcattag tcgcgtcaca
      121 gcctataccc catacggtat cagtgggata aaggatcact cctccttcat tcatcacctg
      181 acaggctttt ttaatatctt ctatcatttc ttgattaatt tccgttaaga acaagacgca
      241 aaaataacac atttattcca tttagtgaaa tctatcctga cgtttttaaa ttacccttta
      301 aaatcaactg ttggcagaca ataatttaac tattacatta ttacaagcgt gtttccctaa
      361 caggacaaac atcccaaaac ttcgttttaa acgattatta acgaaaatct tccttatctc
      421 cgaaagcaga atcattagaa atctctatct ttgcctcaat ttcaagcaag aaagcaaaag
      481 tgccattttt gtgtcgaatt taaaacgttt aagagaaaac aatggaagaa atcgtatttc
      541 atcacacatt acctatccaa ttgcgattca atgatgtaga caaatttggt cacgtcaaca
      601 acacagttta cttctccttt tacgacttag gaaagactga atacttcgct tcagtatgcc
      661 ccggagtaga ttgggaaaag atcgggattg tcgttgtcca tatcgaagcg gatttcgtca
      721 aacagatatt cgcatccgat catatcgccg tacagactgc tgtttctaag ataggcacca
      781 aaagctttca cctgatccag cgggttatcg acacagaaac gaatgaagta aaatgtatct
      841 gcaagtctgt tatggttact ttcgatcttg aaagacatga atccatgcca ctgacagaag
      901 aatggataga ggcgatctgc aaatatgaag aacgggactt gcaaaaagca tagatagatt
      961 ttttgaaaaa gccccctcac gaatgagaaa caaaacaagc aaaaccaatt ctccggaaaa
     1021 agttttattc taattcatgt ccgctatata acagaaattg cctatttttg taccttaatt
     1081 aataaggcat aaaatatcta gtacagaaaa tgatagctga caaaagtata aatttagata
     1141 ccctttataa agtattgttt gataatgaga agctggaact ctctaaagaa tgcatccgaa
     1201 aagtagagga aagcttcgat ttcctacaat ctttttccag cgataagatt atttatggta
     1261 tcaatacggg gttcggccca atggcacaat accggataga agatcagtca ctgatcgaac
     1321 ttcagtacaa tatcattcga agccattcca ccggtgccgg taaaccactt cccgaacttt
     1381 atgtaaaagc agctatgatt gcccgcctgt acacttttct acaggggaaa tcaggagtac
     1441 acatggagct ggtatctctt ctctgtgaat ttatcaaccg tggaatttat ccgttcatac
     1501 ccgaacatgg aagtgtaggt gccagcggcg atctcgtgca actggcccat attgccctga
     1561 cattaatagg agaaggggaa gttttttatc aggggaaatt gcgtgatgcc gctactgtgc
     1621 ttgaggaaaa cggtctgcaa cctttttcaa tgcgcatccg cgaaggatta tccgtcacaa
     1681 acggtacttc tgtgatgaca ggaatcggta tcgtcaatct gatttatgcc aaaaagctgc
     1741 tccgttggtc ggtagccgct tctgtcatga tgaatgagat tgccgcctct tatgatgatt
     1801 tcatggcaca ggcattaaac gaggccaagc atcacaaagg gcaacaagag atagccgcta
     1861 tgatgagaga atgggtcgcg ggcagcaaat gcgtacttca aagagagaat gagctataca
     1921 accaggtgca caaagaaaaa atcttcgagc acaaagtaca gccctattat tccttgcgat
     1981 gtgttccgca aatcctcggt cccatctatg atgagctgaa aaatgcggaa gaagtattga
     2041 ttaacgaaat aaattccgcc tgcgacaatc cgattgtcga tccggacaca caaaatattt
     2101 atcatggcgg taacttccac ggagattaca tctctttcga aatggataaa ctgaaaatcg
     2161 ccgtaaccaa actgaccatg ctttgcgaaa gacagatcaa ttatctgttc cacgaccgta
     2221 tcaatggcat cctgcctcct tttgtaaact tgggagtgct tggattgaac tacggtttgc
     2281 aggcttcgca attcacagca acatccacta cagccgaatg tcagacattg tcgaatccga
     2341 tgtatgtgca cagtattcct aacaacaacg ataatcagga tattgtcagc atgggaacca
     2401 actcggctct attagcaaaa acagtcatag agaattctta tcaggtaatg gctattcaat
     2461 ttatgggaat ggcgcaagcc atcgactacc tgaaaataca ggatcgtcta agtcccaaaa
     2521 gccgtcaggt gtatgaagaa atacgtagtt tcttccctgt atttaccaat gacacaccta
     2581 aatataaaga gatagaaaga atgatggact atctcaaaaa agaaaataaa taaaatgaaa
     2641 tacgcattag taaccggagg aagtcgaggt atcggccgtg ctgtcagttg caaactggcg
     2701 gaaatgggct actttatact gattaattat cagagtaacg acgcagaagc ggaaaaaacg
     2761 ttgcagttag tgcaggaaaa aggaagtaac ggagaaatca tgaagttcga tgtgacagac
     2821 cctgccgcta ttgcccttgc cttgaacaac tgggcgtcac aacatcctga cgaatacatc
     2881 gaagtactga ttaacaatgc gggcatccgc aaagacaacc tgatgctttg gatgacagga
     2941 gaagaatgga acaaagtgct cgatatcagt ctgaacggat tcttttatgt gacacaacct
     3001 ttattgaaga atatgctggt caaacgttat ggacgtattg tcaatatcgt atccttgtca
     3061 ggtattcagg gaatgcccgg acaagccaat tattccgcag ccaaaggtgg cgtaatagct
     3121 gctaccaaag cactggcaca agaagtggcc aagaaaaagg taacagtcaa cgcagttgct
     3181 cctggattta tccgcacgga tatgacggaa gggattgatg agaacgaatg gaaaaaacac
     3241 attcctgcag gacgtttcgg tactgcggaa gaggttgccg atctcgtcgg ttttctggca
     3301 tctccggcct catcctatat cacaggagag gtgatttcca tcaacggagg actatatact
     3361 taaaacaaaa gcatagaata gacaaaataa tgaaaagagt tgttatcacg ggaatgggaa
     3421 tttactcttg catcggcaag aacctggatg aagtaaaaga ctcactatac aatgggaaat
     3481 cggggattgg catagatccg gtacggaaag aactgggcta tttttctgca ttaacgggca
     3541 tattggaacg tcccgacttg aagaaactat tagatcgtcg taaacgtctc tgcctgccgg
     3601 aacagggaga gtatgcttat ctcgctactt tggaggcttt ccgcaacgcc ggtattgacg
     3661 atgcttttct cgaagctaat gaagtgggta tcttgtatgg caatgatagt agtgctgccc
     3721 ctgtcatcaa cgcagtggac atcattcgtg aaaagaaaaa cacagcgttg gtaggttccg
     3781 gttctatttt ccagtcgatg aattctaccg tgacaatgaa cctgtcggtt atcttcaagc
     3841 ttagaggagt taacttcacc attgccggag cttgtgcaag tggttctcat gctattggca
     3901 tggggtatct gctcattaaa tcaggattgc aggattgtat tctttgcggt ggcgcacaag
     3961 aagtaaatcc ttatgcggtc ggcagtttcg acgggctaag cgcattttcc acccaggaag
     4021 cagagcccga gaaagcctcc aaaccttttg ataaacgacg tgacggactg attccgagtg
     4081 gaggagccgc cagtctggta ttagagagct atgaatcggc agtaaaaagg ggagctccca
     4141 ttttggccga agttatcggt tacggtttct cttccaatgg agatcatatc tccgtaccga
     4201 acgtggacgg tccgaaacgt tctcttcaga tggccatcaa agatgcgggt attgcactcg
     4261 aacaaatcag ttatatcaac gcacacgcca cttccactcc ggtaggcgac ctgaacgaag
     4321 cgaaagccat tgcggaagta tttgaaggac atcacccgta tgtcacttcc acgaagtcta
     4381 tgaccggaca tgaaatgtgg atggcaggtg ccagtgaagt gatttactcc atcctcatga
     4441 tgaataacgg tttcatcgct cccaacctca atttcgagga agcggacgag gcttccgcac
     4501 agttgaatat acctacccaa cgggtaaatc tggaatttga tacgttcctt tccaactcgt
     4561 ttggattcgg aggaaccaat tccaccctga ttatacgcca aatcaaagaa taaattacta
     4621 aagaaaacag atatatgaca aacgaagaaa tcatcgagaa aataagaaca actctggcag
     4681 aagagtttga agtagacatc gacgtcatcc agccggatgc gccgttaatg gaaactcttg
     4741 aactggacag tctggacttg gtcgacatgg tggtactggt agaaaagaat ttcggtttca
     4801 acgtaaccgg acaagacttt gcaggtatca aaaccttcca ggacttctac gatctcgtta
     4861 tcacccgtat gcaggaagcc aagtaaatat gtcgacgtgg aaaggcaaaa cccggggagg
     4921 aacgttcgga tacttattct ttatttacct gattaagtat ctgggcatta ctgccgctta
     4981 tctttttcta agcctggtag tgctctactt cataccgttt gctccgaaag caaccaaaag
     5041 cacctggttt tatgcccgtc acattctgaa atacaatagg atacgttctt tgaaaatgtt
     5101 actactcaac tattaccgat taggacaaat cctgattgac aaggtagcca tcggtaatgg
     5161 aaaagtaaat caatatcagt tcaaattcga gcagtatccg gagtttctgc aactgctgaa
     5221 cagtgaacag ggagtgatta tgattggcgc ccatgtggga aactgggaaa taggcgttcc
     5281 tttctttgat gattacggaa agaaaatcaa tatcgtgatg tacgatgccg aacatcgtag
     5341 aatcaaagag atcctgg
//
