LOCUS       c00770_gnlPro..        14968 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LMOPJJMM_770.
ACCESSION   c00770_gnlPro..
VERSION     c00770_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 00:03:15
            Original ID  :: gnl|Prokka|LMOPJJMM_770
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14968
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14968
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[108:4968]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      109..4968
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..14968
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14968
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             109..1341
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg770_1"
                     /sec_met_domain="HBT (E-value: 2.4e-18, bitscore: 56.5,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDAKEISKRTLAALLKVQQMGVRIVLASGR
                     PTYGLMPLAKMLELGNYGGFILSYNGCQIINAQNGEILFERRINPEMLPYLEKKARKNG
                     FALFTYHDDTIITDSPENEHIQNEARLNNLQIIKEEEFSAAVDFAPCKCMLVSDDEEAL
                     VGLEDHWKRRLNGALDVFRSEPYFLEVVPCAIDKANSLGALLEVLDVKREEVIAVGDGV
                     CDVTMIQLAGLGVAMGHSQDSVKACADYVTASNEEDGVAVAVEKAIIAEVRAAEIPLDQ
                     LNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALAETVAGLGS
                     MILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVHKGRSSHVWNVDVFTSTNKLVSS
                     IRVVNSVMKKR"
     CDS             1338..2453
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg770_2"
                     /sec_met_domain="Chorismate_bind (E-value: 4.4e-57,
                     bitscore: 183.6, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRIPGEKVPRLLTQAEGVVRL
                     IYDLKELNGQRGFVIAPFQVNESCPVVLIQPDQWGQPLPVDDDTEEDREIALRLQGQES
                     FLTSSTEEYTACFHTFINALRDRTFDKLVLSRHLTVDKVADFSPSSIFRAACKRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWHTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     SSYIRRQLLSLDIHATENGPYPAYAGALSHLKTDFRFALKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYQFILQNEGYDRRYYSGFIGWLDPEGRTDLYVNLRCMHIEDKQLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLIAVPPLKS"
     CDS             2473..4140
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg770_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.4e-07, bitscore:
                     21.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYPVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVIISADR
                     PAAWIGQMDGQTVPQPGVFQTLVKKSVNLPEIHTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINIPISEPLFQFTVDSLPEVRVITRYQGLNVYDRDYNDLVDRMNKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKVDQMVPELLIT
                     YGGHVVSKRLKKLLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPEYPRVWENYCKIIPEPEFAYSEMSAVGTLLKALPESCALHLANSSVVRYAQLYSIP
                     STIEVCCNRGTNGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNVNVRSNLRV
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELAEAM
                     KTFTQPEAMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             4144..4968
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg770_4"
                     /sec_met_domain="ECH_1 (E-value: 1.5e-82, bitscore: 266.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKQYPKFP"
     CDS             5141..6166
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg770_5"
                     /sec_met_domain="MR_MLE_C (E-value: 2.7e-28, bitscore:
                     89.1, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKIEIIPRLLHFKQPAGTSRGTYTTRKVWYLHFTSPDFPGRVG
                     IGECAPLPALSCDDLPDYEDILVKVCRKVEREQGRLDMDGLCDYPSILFGLETAIRHFF
                     AGNWALCDTAFSRGEVGIPINGLIWMGDFQNMLSQIEKKMEAGFRCIKLKIGAINFEEE
                     LTLLRHIRTHFSSKEIELRVDANGAFSPVDAMEKLKRLSEFDLHSIEQPICAGQWEEMA
                     RLAAESPLAIALDEELIGCNTLERKQELLSTIRPQYIVIKPSLHGGICGGDEWITEAEK
                     QQIGWWITSALESNIGLNAIAQWCATFSNPLPQGLGTGALFTDNVEVPLEIRKDCLWFC
                     K"
     CDS             6188..7324
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 57.1; E-value: 4e-18)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg770_6"
                     /sec_met_domain="AMP-binding (E-value: 1e-18, bitscore:
                     57.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFDRKQQRLLLEGKEYTFEDISRLIAGGAEAHSPASWDLYLFLN
                     EWFNDSPVIIVHTSGSTGTPKELIVRKDQMMQSARLTCEFLDLKKGETALLCMNLRYIG
                     AMMVVVRSLVAGLNLIVRPASGHPLADIKEPLRFVAMVPLQVYNTLQVPEEKERLKQTD
                     ILIIGGGAIDEALEAEIKHLPTAVYSTYGMTETLSHIALRRLNGTLASEHYYPFSSVKL
                     SLSVENTLIIDAPLVCDEILQTNDIARIYPDGSFMILGRKDNVINSGGIKIQAEEMEKL
                     LRSFIPVPFVITSVPDQRLGQAVTLLLEGQPELLEGQPDTKEIGNKLQEILEPYYRPKY
                     IWTTDCIPQTENGKVNRAKCRILAKQMLTTFYTHTLTP"
     CDS             complement(7635..8111)
                     /locus_tag="ctg770_7"
                     /transl_table=11
                     /translation="MKTLEFIKLNESGANNVVASLQQLLADFQVYYTNLRGFHWNIKGH
                     DFFVLHSQFEKMYDDTAEKVDEIAERILMLGGTPANKFSDYLKVANISEVDKVSNGEQA
                     LNNILQSISYLIGEERKILSIASQAGDEVTVSMMSDYLKEQEKLVWMLTAYNSK"
     CDS             8249..9175
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     61.1; E-value: 2.4e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg770_8"
                     /sec_met_domain="LysR_substrate (E-value: 2.8e-44,
                     bitscore: 141.0, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 6.2e-20, bitscore: 61.1,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIQQLEYILAVDQFRHFARAAEYCRVTQPTLSAMIQKLEDELGV
                     KLFDRTVQPVCPTPIGQKVIDQARVILAQAAQVKEIISEDKQSLSGVFRLGVLPTIAPY
                     LLPRFFPQLMEKYPELDIRVTEMKTQDIQQALHAGDLDAGIIASKLEDTFLTEETLFYE
                     QFYAYVSRKEPSFKHDVIRTSDITGEHLWLLDEGHCFRDQLVRFCQMEAVKVSQMAYRL
                     GSMETFMRMVESGKGITFIPELAVAQLTEEQRQLVRPFAIPRPTRQVVLATNRDFIRHS
                     LLCVLKEEIKAAVPKEMLTLQSIQCLL"
     CDS             9489..10178
                     /locus_tag="ctg770_9"
                     /transl_table=11
                     /translation="MKKYIAEMIGTMVLVLMGCGSAVFAGSVTGTVGAGVGTVGVALAF
                     GLSVVAMAYAIGGISGCHINPAITLGVFLTGRMNGKDAGMYMIFQVIGAIIGSAILFAL
                     VSTGAHDGPTATGSNGFGDGEMLQAFIAEAVFTFIFVLVVLGSTDSKKGAGNLAGLAIG
                     LTLVLVHIVCIPITGTSVNPARSIAPALFQGGEALSQLWLFIIAPFVGAALSAVVWNYL
                     GDKEEKK"
     CDS             complement(10318..11040)
                     /locus_tag="ctg770_10"
                     /transl_table=11
                     /translation="MKKFKFIAFIFAIMTTMPILQSCLDDDDNPSDLLVVSTINMISQD
                     SKEFYFTLDDGKKMYPSNAQGWSNKDWVEGQRAFVIFNELEEPVNGYDLNIQVRGINPI
                     LTKDIITMGEDDNDEEKVGDDKINTTYMWINKDNKYLTIEFQYYGTHSEDKKHFLNLVI
                     NDKEESAPTADEDSAEDEYINLEFRHNSEGDYPQSLGEGYVSFKLDKIKGRMEGKKGLR
                     IRVNTLYGGPKTYEVKFP"
     CDS             11217..11765
                     /locus_tag="ctg770_11"
                     /transl_table=11
                     /translation="MEELELSEQCRLGNNQARKELYEQYAGRMLGICLRYTGDRDTAQD
                     LLHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQYLRKNDVINQSAPLEELPEEY
                     EEPDASDVEAIPQKVLMQFIEELPAGYRTVFNLYTFEDKSHKEIAQELGINEKSSASQL
                     FRAKSVLAKRVKEWIMHNG"
     CDS             11772..13070
                     /locus_tag="ctg770_12"
                     /transl_table=11
                     /translation="MEEKELWMNKLKEKLADYSEPVPASGWEQLEKELMPPVEKKIYPY
                     RKWMMAAAAVILLAVVSSVSLYFLGTPAADEIRHIKTPALASTPDALPGVQQPDVQGTS
                     VEPVLRPVAREDRLAKIDKNLTEQKTNAGQSAIENNNEPVTGNENNPVIEEDQMLKGET
                     EQTKNEARQVDSENVGQATQSQDTKRPNNRPRRPSGRDKLHIPAEKRASQKGTWSMGLS
                     VGNSGGASTEVGAGSHAYMSRVSMLSVSNGLMEIPNDQTLVFEDGVPYLRQAKQVVDIK
                     HHQPISFGLSVRKGLAKGFSLETGLTYTLLSSDAKLAGEEQQIEQKLHYVGIPLRANWN
                     FLDKKLVTLYVSGGGMVEKCVYGKLGSEKETVKPLQFSVSGAVGAQVNATKRLGIYVEP
                     GVAYYFDDGSDIQTIRKENPFNFNIQAGIRLTY"
     CDS             13283..13879
                     /locus_tag="ctg770_13"
                     /transl_table=11
                     /translation="MVNFTDEKHLLIDLKDGSFQAFERLYNMYSGKLYNFIMRLSSGNQ
                     YMAEEVVQSTFIRIWEVREKVDTNASFISFLCTIAKNLLMNMYQRQTVEYVYNEYLLKS
                     GLDHDSQTEDTIDLRFLNDYIDSLAEELPAQRKKIFILSKRQNYTNKEIAEMMGISEST
                     VATQLSLAVKFMREQLMKHYDKVIALLIAFFVNEM"
     CDS             13947..14927
                     /locus_tag="ctg770_14"
                     /transl_table=11
                     /translation="MDKIYYKELIEKYFDGNITDAEIKKLSDWIKNDRHLQNWWEEEFS
                     KSDAGINPVLRDKLFARIKEQTQGKEETQGKENPRTIRMNPWKWAAAIVLPICIAFFTY
                     YLVDSSQTVGAPFIVKADKGDKATIELPDGTNVVLNSASQLSYLNNFGENGRRVQLNGE
                     AYFKVAHDEKCAFIVQVGDLEVKVLGTSFNVSAYEDAKDVTVVLLEGKVGVYAQKTSHI
                     MKPGDKIEYNKATHKITATQVHPSDYIEWTKGNIYFEKESLENIMKTLSRIYDVEIRFD
                     SNKLPNEYFTGTIPGGGIQNALNILMLTSPFYYEMDGSVIVLKEK"
ORIGIN
        1 ctggaaatgc gtttgaaaga gtgaaattaa agtaattttt atctgtttac gctttctgtc
       61 gttcactcaa tttcctacct ttgtaatcta aatcaaaatc gtgtaatcat gaagtataaa
      121 ttattggttc ttgatgtaga cggaacactc ctcaatgatg cgaaagaaat tagtaagcgt
      181 acattggctg ccctgttgaa ggttcagcaa atgggagtac gtattgtgct ggcatccggc
      241 agacctactt atggcctgat gccgctggcc aagatgcttg aacttggaaa ctatggaggt
      301 tttatccttt cctataatgg ctgtcagatt atcaatgcac aaaacgggga aattctgttt
      361 gaacgtcgga ttaatcccga aatgttgccg tatctggaaa agaaagcccg taaaaacggt
      421 tttgccttat tcacttatca tgatgatacg atcataacgg attctcctga aaatgaacat
      481 atccaaaatg aagcccggtt gaataacctg caaataatca aagaagaaga attttctgct
      541 gccgttgatt ttgctccttg taagtgtatg ctggtgagcg atgacgaaga agcactggtt
      601 ggtctggaag atcactggaa gagacgattg aacggagcgt tggacgtatt ccgttcggaa
      661 ccatattttc tggaagtagt tccctgcgct atcgacaaag ccaactcttt gggtgctttg
      721 ctggaggtac tggatgtgaa gcgtgaggaa gtgattgctg tgggcgacgg tgtgtgtgac
      781 gtaacaatga ttcagttggc cgggctgggc gtagctatgg gacattcaca agactcggtg
      841 aaagcctgtg ccgactatgt gactgcttcg aatgaggaag acggagtggc cgttgcagtg
      901 gagaaggcta ttatagccga agtgcgtgca gccgaaattc cccttgacca actgaatgcg
      961 caggcacgcc atgcattaat gggaaacttg ggaattcaat atacatacgc cgacgaagat
     1021 cgtgtggaag caacgatgcc ggtagaccat cgtacccgtc agccttttgg tattttgcat
     1081 ggtggagcta ctcttgcgtt ggcggaaaca gttgccggac tcggttcgat gattctttgc
     1141 cagccggacg agattgtagt aggaatgcag gtcagcggaa accatatatc ttctgcacat
     1201 gaaggagata ccgtgcgtgc agtaggaaca gttgtacata aaggacgttc ctcccacgta
     1261 tggaatgtag acgtgtttac ttcaacaaac aaactggtgt cttccatacg ggtagtcaac
     1321 agtgtgatga aaaagagatg accgacgaag aaataagtaa cttgacaact attgatgcat
     1381 ttattcagcg gaagcagccg tttgctgttt accgtattcc cggagagaaa gttccccgtc
     1441 tgttgacgca ggcggaagga gtggtccgtt tgatatacga tttgaaagaa ctgaacggac
     1501 agagaggatt tgtaattgct ccgtttcagg tgaatgagtc atgtccggtt gtattgattc
     1561 aacccgatca gtgggggcag cctttaccgg tggatgatga tacggaagaa gaccgggaga
     1621 tagccttgcg cctgcaaggg caagaatcat ttctgacttc ttccacggaa gaatatacgg
     1681 catgctttca tacctttata aatgcgttac gtgacaggac ttttgataaa ctagtgcttt
     1741 cccgtcatct tacagttgat aaagtagctg atttctctcc ttcgtccatc tttcgggcgg
     1801 cttgtaaacg ttatattcat tcgtatatat acttatgtta tactccgcag acagggatct
     1861 ggctgggaag cactcccgaa atcattttat cgggtgagaa agacgaatgg catacggttg
     1921 cgctggccgg aacacagcct ttgcaagatg gcagactgcc gcaagtatgg gatgaaaaga
     1981 accggaagga acaggattat gtatcttctt atatccgtcg tcagcttctt tcactggata
     2041 tccatgctac ggaaaacggg ccatatcctg cttatgccgg tgctttgtca catttaaaaa
     2101 ccgatttccg gttcgctttg aaagataata aaggtttggg agaccttctg aaagttttgc
     2161 atccgacacc tgccgtatgc ggcttgccga aggaagaagc ctatcagttt attttgcaga
     2221 acgaaggtta cgaccgccgc tattactccg gttttatcgg atggctcgat ccggaagggc
     2281 gaacagactt gtatgtaaac ctgcgctgta tgcatatcga agataaacaa ctcactcttt
     2341 atgccggcgg tggattattg gcctcttcgg aactgaatga cgaatggctg gaaacggaga
     2401 aaaagttgca aaccatcaaa cggctgattg cagtgcctcc tttaaaatca taaactacta
     2461 ataattatca tcatgtatac agacaagaag aacatactcc agttggttgc gttgcttgag
     2521 gcgcatggga ttactaaggt tgtgttgtgt ccgggtagtc gtaacacccc catagtgcat
     2581 actttgtcta accatccgaa ttttacctgc tatccggtga cggatgaacg gagtgccggc
     2641 tattttgcca tcggtcttgc gttgaatggt ggtaagcctg ctgctgtctg ctgcacttcc
     2701 ggtactgcat tattaaacct tcatccggct gtggcagaag cattttatca aaatgttcct
     2761 ctggtcatta tttctgcaga ccgtcccgct gcctggatcg gacaaatgga cggacagact
     2821 gtaccacaac caggcgtatt tcagacgctg gtcaagaagt cggtaaacct tccggagatt
     2881 catacggaag aagatgaatg gtattgcaat cgtctggtga atgaagcatt gctggagacg
     2941 aaccatcatg gaaaaggacc ggtacatatc aatatcccta tttctgaacc attgttccag
     3001 tttacggtcg attcgcttcc agaagtgcgt gtcataaccc gttatcaggg gttgaacgtc
     3061 tacgatcgtg attataacga tttggtcgac cggatgaata aataccagaa gcgtatgatt
     3121 atcatcggtc agatgaatct tatctatctg tttgaaaaaa gatatatcaa gttattatat
     3181 aaacattttg cctggttgac ggaacatatc ggcaaccaga cagttcccgg tattccggta
     3241 aagaacttcg atgcggcgct ttatgccatg cccgaagaga aggtagatca gatggttccg
     3301 gagctgctga ttacttatgg aggacatgtc gtttccaaac gattgaagaa gttgctccgt
     3361 cagcatccgc ctaaagaaca ttggcacgta tcgccggacg gtgaggtggt cgatttgtat
     3421 ggctcattaa ctacggtgat cgaaatggac ccgttcgagt ttctggagaa gatagccagt
     3481 ttgctggaca atcggactcc tgaatatccc cgtgtgtggg aaaactattg caagatcatt
     3541 cctgaaccgg agtttgctta ttcggaaatg tctgctgtcg gtacgttgtt gaaagccttg
     3601 ccggagtcat gtgcgttgca tctggctaac agttcggtgg tgcgctatgc gcaattatac
     3661 tccattcctt ctacgattga agtttgttgc aaccggggaa caaacggcat tgaaggctcg
     3721 ctgtctacgg ctgtcggtta tgcggcagct tccgacaaac tgaactttat agctatcgga
     3781 gatttgagct tcttctatga tatgaatgcg ttgtggaacg tcaatgttcg ctccaatctg
     3841 cgtgttcttc tgttaaataa tggaggagga gagattttcc ataccttgcc gggattggat
     3901 atgtcgggta cctcacataa atttattgcg gcagttcata aaacatcggc caaaggttgg
     3961 gcagaagagc gtgggtttct ctatctgcaa gcgcaaaatg acgaagaact ggctgaagcg
     4021 atgaaaactt tcactcaacc ggaagctatg gagcagcctg tgctcttgga ggtgtttacc
     4081 aacaaaaaca aggacgcgcg catgttgaag aattattatc atcaattaaa acaaaaatag
     4141 attatgtcaa cacaaagaga gtggacaacc attagagaat acgacgatat tctttttgat
     4201 tattataatg gaattgcccg tatcacgatc aaccgtgagc gctatcggaa tgcgtttact
     4261 ccgacaacta ctgctgaaat gagtgacgca ttgcgcatct gccgtgaaga agcggatatt
     4321 gatgtgattg tgattaccgg agccggtgat aaggcgtttt gttcgggtgg cgaccagaat
     4381 gtgaaaggac gtggcggata tatcggaaaa gacggtgtgc ctcgtctaag cgtattggat
     4441 gtgcaaaagc agattcgcag catcccgaaa ccggtgattg ctgccgtgaa cggttttgct
     4501 attggtggcg gacatgtgct tcatgtggta tgtgatttgt ccattgcgtc ggaaaatgct
     4561 attttcggtc agacgggtcc tcgtgtaggt agctttgatg ccggattcgg tgcgtcttat
     4621 ctggcacgtg tggtaggtca gaagaaagca cgcgaaatct ggttcctctg tcggaagtat
     4681 aatgcacaag aagcattgga catgggatta gtgaataaag tagttccttt ggagcagttg
     4741 gaagatgaat atgtgcagtg ggcagaagaa atgatgctgc ttagtccgtt ggctttgcgc
     4801 atgattaaag caggattgaa tgccgaactg gatggtcaag caggtattca ggaattggca
     4861 ggtgacgcaa cgttgctcta ttacctgact gatgaagcac aggaaggaaa gaatgcgttt
     4921 ctggagaaac gtaaaccgaa cttcaaacaa tatccgaaat tcccttaaaa tcggctttgc
     4981 cacagagtac cacagagttt gcttttcctg cgtttaagcc cgattttctg caaagcaaga
     5041 ctgtggagga gagaatggta ggagaactgc aagagatata acttattaaa acttcgtgag
     5101 gctctgtgtt attctgtggt gaattgaaaa attattcacg atgaattgta aaatagaaat
     5161 tattccccga ttgctccatt tcaagcaacc tgccgggact tccagaggaa catatactac
     5221 gcgtaaagtt tggtatcttc attttacctc tcctgacttc cctggcagag tgggaatagg
     5281 ggagtgtgca cctttgcccg ctttaagttg tgacgatctt cctgattacg aagatatatt
     5341 agtgaaagtc tgccggaaag tagagaggga acaaggcaga ttggatatgg atggcttgtg
     5401 tgactatcct tcaattttat ttggtctgga aacggccatc cggcatttct ttgcaggaaa
     5461 ctgggcttta tgtgatacgg ctttctcacg tggagaagtt ggtattccga ttaacggcct
     5521 tatttggatg ggagattttc agaacatgtt atcccagatt gagaaaaaga tggaagccgg
     5581 atttcgttgt atcaagttaa agataggtgc catcaatttc gaagaagaac tgacattgtt
     5641 gcgccatatt cgtacgcatt tctcttctaa agaaatagag ttgcgtgtag atgcaaatgg
     5701 tgctttctca cccgtggacg caatggaaaa actgaaacgt ctgtccgagt ttgatttaca
     5761 ttctatcgaa cagccaatct gtgccggaca atgggaggaa atggctcggc ttgctgccga
     5821 atctccatta gcgattgctt tggatgaaga actgatagga tgcaatacgt tggaaaggaa
     5881 acaggagctg ctttcaacaa ttcgtccgca atatatcgtt attaaacctt ctcttcatgg
     5941 aggaatatgc ggaggagacg aatggattac cgaagctgaa aaacaacaga tcggctggtg
     6001 gataacttcc gctctggaat caaacattgg gctaaacgcc attgcccagt ggtgtgcaac
     6061 attcagcaat ccgttgccac agggactggg aacaggggcg ctctttacag acaacgtgga
     6121 agtgcctctt gaaataagaa aagattgttt atggttttgt aaatgatagt atttaaaaga
     6181 tattccgatg atattcgatc gaaaacaaca acgcttgtta ctggaaggaa aagaatatac
     6241 ttttgaggat ataagccgat taatagcagg aggagcggag gctcattctc ctgctagttg
     6301 ggatttatat cttttcctga acgaatggtt taacgattct cctgtcatca ttgttcatac
     6361 atcaggctct acgggtacac ctaaagagtt gattgtacgt aaagaccaga tgatgcaaag
     6421 tgcgcgtctg acttgtgagt ttctggattt gaagaaagga gagacggcat tactttgtat
     6481 gaatcttcgt tatattggtg caatgatggt ggtcgtgcgt tcgttggttg caggcttgaa
     6541 tttgattgta cgtcctgctt ccggtcatcc tttagcggat ataaaagagc ctttgagatt
     6601 tgtggcaatg gttcccctgc aagtttataa tacgttgcaa gttcccgaag agaaagaacg
     6661 attgaaacag acggatattt tgattatagg tggtggagct attgatgagg ctttagaggc
     6721 ggagatcaaa catcttccga ctgccgtcta ttctacctat ggcatgactg aaactttatc
     6781 tcatattgct cttcgtcggc tgaacggaac attggcttcg gaacattatt atccgttttc
     6841 ttcagtaaaa ctgtctttat ctgtagagaa tacgttaatt attgatgccc ctttggtatg
     6901 tgatgaaata ttgcaaacca atgacattgc acgcatctat cccgatggaa gttttatgat
     6961 tttgggtcgg aaagacaatg tgataaatag tggaggaatt aagattcagg cggaggaaat
     7021 ggagaaattg cttcgttctt ttattcctgt gccatttgtt attacttcag ttccggacca
     7081 gcgtttaggg caggctgtaa ctttactatt ggaaggtcag ccagaattat tggaaggtca
     7141 gccggacact aaagaaatcg gaaacaaact acaggaaata ttggaacctt attatcgtcc
     7201 caagtacata tggacgacag attgtattcc gcaaacagaa aacggcaagg ttaatcgtgc
     7261 gaagtgtcgt attttagcca aacaaatgtt gacgacgttt tacactcaca ctctcacacc
     7321 ttgattgaaa tcctttattc atcaacgttt caggggcctc taattgtctt accctagttt
     7381 tagttgagac ttattagagt caacaaaatt cagggtaaga cattttattt cagtagaata
     7441 agtttcatct tttgaaagtt gttgcttact tttataaatc ttgataaata aagaacaaaa
     7501 ctaatttttt taataatatc agcgaatgaa cgtatttaga tagtactagt acactggaaa
     7561 taataaagag agaatattcc ttttacggaa atatcctctc tttctcactt aaaaacctaa
     7621 accttttatc ttaattattt gctattataa gccgtcaaca tccaaactag tttttcctgt
     7681 tctttcagat aatcgctcat catcgaaacg gttacttcat ctccggcttg agaagcgata
     7741 gacaagattt tacgttcttc accaatcaga taactgatac tctgaaggat attgttcagt
     7801 gcctgttcgc cgttgctcac cttatctact tcactgatat ttgctacttt caaataatcg
     7861 ctgaatttat tggcaggagt accgcccaac atcaagatac gttctgcaat ttcgtctacc
     7921 ttttcggctg tgtcatcata catcttttcg aattggctgt ggagaacaaa gaaatcatgt
     7981 cctttgatat tccagtggaa accgcggaga ttggtgtaat acacttggaa gtctgctaat
     8041 agttgttgca aagatgctac tacgttgttt gctcccgatt cattcaattt gataaattct
     8101 aaagttttca tattcttttt atttttaatg atttatactt gttttctttt tttctgtact
     8161 gcaaagataa ggcggaaaat gcttgttgtc aaactgaaaa tttctatctt tgcatagatg
     8221 aaatctataa cttaacatga tttgatagat gacgatacaa caattagaat atatattagc
     8281 cgtagaccag ttcaggcatt tcgcacgggc agccgaatat tgccgtgtga cgcaacccac
     8341 tttgagtgcc atgattcaga agctggaaga tgaattagga gtaaagttgt tcgataggac
     8401 agtgcaaccc gtctgcccga cacccatcgg gcagaaagtg atagaccagg ctcgtgtgat
     8461 tctggcgcag gcagctcaag taaaagaaat catcagtgaa gataagcaat cattatcagg
     8521 tgtattccgt ttgggagtat taccaactat cgctccttat ttgctacctc gctttttccc
     8581 tcaactgatg gagaaatatc ccgaactgga tattcgtgtc acggaaatga aaacccagga
     8641 tattcagcag gctctgcatg ccggtgattt ggatgcaggg attattgcca gtaaattaga
     8701 agacacattc ttgacggaag aaactctctt ctatgagcaa ttctatgctt atgtatcacg
     8761 gaaagaaccc tcgttcaaac atgacgttat ccgcacttcc gatataaccg gcgaacatct
     8821 ttggcttttg gacgaaggac attgttttcg tgaccaactt gtccgtttct gtcagatgga
     8881 agcggtgaag gttagtcaga tggcttatcg tttgggaagc atggaaacct ttatgagaat
     8941 ggtagaaagc ggaaaaggaa tcacttttat tcccgaactg gcagtggctc aattgacgga
     9001 agaacagcga caattagtac gtccgtttgc tatcccccga ccgacgcggc aagtggtgct
     9061 ggcgacaaat agagatttta ttcgtcacag cctgctatgt gttctgaaag aggaaataaa
     9121 agcagcagtc cccaaagaaa tgcttacttt gcagtccata cagtgtttgc tataataata
     9181 gagattgaga tgattcctgt catataccct tgtaccgggt ataagcgaaa agaataattc
     9241 caacagtggc aaagcgggtt tcggagaaat tcatcggagt ggcgtcattg ctcaatcctt
     9301 gcttgataaa agagtttacg atgtgcccat aaggtagagg ctaatatggt gtgcaaaagt
     9361 ccttttttca ttttatttgc ttgtctttta ttcaaaaatg ttgcgaaggc aataataatt
     9421 taggcacgta ggttttgttt tcttgggtta attctctata tttgatgcat taaccattat
     9481 ttatagttat gaaaaagtac attgcagaaa tgattggaac gatggtgctc gttcttatgg
     9541 gatgcggaag tgccgtcttt gccggtagtg taaccggtac ggtaggtgct ggcgtgggaa
     9601 cagtcggcgt agctttagca tttggtcttt cagtagtagc gatggcgtat gctatcggag
     9661 gtatttcagg atgtcacatt aatcctgcta ttacattagg agtgttctta actgggcgta
     9721 tgaatggaaa agatgcaggt atgtatatga ttttccaggt aatcggagct attatcggct
     9781 cggctattct ttttgcttta gtttccaccg gtgctcatga cggacctacg gctacaggtt
     9841 ccaatggttt tggcgatgga gaaatgttgc aggcattcat tgcggaagca gtatttactt
     9901 ttatttttgt attagtggtg ctcggctcta ccgactcgaa gaagggagca ggcaatctgg
     9961 caggacttgc catcggtttg actcttgtat tggtacatat tgtatgtatt cctatcacag
    10021 ggacttctgt aaacccggcg cgtagtattg ctccggcatt attccaggga ggagaagcat
    10081 tgtcgcagtt gtggctgttc atcattgctc catttgtagg agctgcgttg agtgcggtag
    10141 tttggaatta tctgggagat aaggaagaaa agaaataatt cctttataag gataacttct
    10201 ttatataaag gagaagataa aagggcggca gtttcacaac taccgccctc ttttgcgtaa
    10261 ttaatatatt atattaaaaa agagtgtgtt ttgcacgttt acattagatt tataatctta
    10321 gggaaacttt acttcataag ttttcggtcc gccatataaa gtgttgacac ggatccttaa
    10381 tccttttttc ccttccatcc ggcctttaat cttatccagt ttgaaagata catatccttc
    10441 gcccaggctt tgaggataat caccttcact gttatggcgg aattccagat tgatatattc
    10501 gtcctccgca ctgtcttcgt cagcagtggg agcagactct tctttgtcgt tgattacgag
    10561 attcaggaaa tgttttttat cttcactatg cgttccataa tattggaatt caatcgttag
    10621 atatttatta tccttgttga tccacatata agttgtgttg atcttgtcat ctccgacttt
    10681 ttcttcgtcg ttgtcatctt cacccatcgt gatgatgtct ttagttaaga ttggattgat
    10741 acctctcacc tgaatattaa ggtcgtatcc attcacaggc tcttccagct cgttgaatat
    10801 cacaaaagct cgttgtcctt ccacccaatc tttattactc catccttgcg cattgctggg
    10861 atacattttt ttcccgtcat ccaaagtgaa ataaaattcc ttgctatcct ggctaatcat
    10921 attgatggta ctgacaacca gaagatcgga cgggttgtca tcgtcgtcca aacatgactg
    10981 gagaatcggc attgtagtca taatagcaaa gataaaggca atgaatttaa atttcttcat
    11041 atacttattt aagttttaga ttatataaaa tggttccttt gtattgtgaa aatgcaatgg
    11101 aatagaaaat attgcatttg aatctgttaa agaaacaaaa gtgataaaag agttgttctc
    11161 tctatgcagt atttctatct ttgctgcatt tataagttaa acacgtgtat aagagaatgg
    11221 aagaattaga gttgtcggaa caatgcaggc taggcaataa ccaagctcgc aaagaactgt
    11281 atgagcagta tgcgggacgt atgcttggca tctgtcttcg ttataccggc gaccgtgata
    11341 cagcccagga tctgcttcac gacggttttt taaagatatt cgattccttc gacaagttca
    11401 cctggagggg tgaaggctcc ctgcgggcct ggatggaaag ggtgatggtc aacacagctt
    11461 tgcaatatct ccggaaaaat gatgtaatca atcagtcggc accattagaa gagttgcctg
    11521 aagaatatga agaaccggat gcttctgatg tagaagctat tccacagaaa gtgttgatgc
    11581 agtttataga agaactacct gccggatatc gtacagtgtt taacctttat acattcgaag
    11641 acaaatcgca taaagaaatc gcacaggaat taggtattaa tgaaaaatct tcggcttcgc
    11701 aactatttcg tgcgaagagt gtattggcaa aaagagtaaa agaatggata atgcataatg
    11761 gatagataga gatggaagag aaagaattgt ggatgaataa gttgaaggag aagctcgcgg
    11821 attattccga gccggtgcct gcttccggtt gggaacaact ggaaaaagaa cttatgcctc
    11881 ctgtggagaa gaaaatatat ccttatcgaa aatggatgat ggctgctgca gctgttatat
    11941 tgttagccgt cgtttcgtcg gtaagtctat attttctggg gacaccggcc gcggatgaaa
    12001 tacgtcatat aaagactccg gcattggcat ctacacccga tgcgttgccg ggtgtgcaac
    12061 aaccggatgt gcagggcact tccgtagaac ccgtcttgcg accagtggct cgtgaagacc
    12121 gtttggcgaa aattgataag aatcttacag aacagaaaac aaatgcaggc caatctgcaa
    12181 tagaaaataa caacgaacct gtaacaggga atgaaaataa tcctgtgatt gaggaagacc
    12241 agatgctcaa aggtgagacg gaacagacga aaaatgaagc aaggcaggtt gacagtgaaa
    12301 atgtgggtca ggctacacag tcacaggata ctaaacggcc gaacaacagg ccgcgtcgtc
    12361 cttccggcag agacaagctg catattccgg cagaaaaaag agcatctcaa aaaggaacat
    12421 ggtccatggg actctcggtc ggaaattcgg gcggagcatc cacagaagtc ggtgccggct
    12481 ctcatgctta tatgtctcgt gtgagtatgc tttctgtttc caacggtctg atggagatac
    12541 ctaatgatca gacattggtt tttgaagatg gagtgcctta tctgcgtcag gcaaaacaag
    12601 tggttgacat taagcaccat caacctattt ctttcggatt gtccgtccgt aaaggactgg
    12661 ccaaaggatt ctctctcgaa acgggattga cttacacatt gctttcttcg gatgcgaaac
    12721 tggcaggtga agagcagcag atagaacaaa aacttcatta tgtaggtatt ccgctgcgtg
    12781 ccaactggaa tttccttgat aaaaaactcg tcactctcta cgtttccggt ggaggtatgg
    12841 ttgagaagtg tgtgtatgga aagcttggtt ccgagaaaga aacggtgaaa cctttgcaat
    12901 tctcggtatc tggtgcggta ggtgcacagg tgaacgcaac gaaacgtctg ggtatctatg
    12961 tagaacccgg tgttgcttat tattttgatg acggttcgga tatacagacc atacgtaaag
    13021 agaatccgtt taatttcaat atacaggctg gtatccgctt gacttattaa tgcgaactac
    13081 cttactgaac atagtgacgg tatccgttca agatggcgtc actatcctga ttagatagcg
    13141 tcactatctt gaccggatgg cgtcactatc ctgatattag tatctttttc ctcataaaaa
    13201 tcggataatt gagaaaatat taataatatt gtcacctgtt aatggtctat ggttgcatac
    13261 ctttgttgca aaattctaag ctatggtgaa ttttacagat gaaaaacatt tattaatcga
    13321 tttaaaagac ggttcttttc aagcatttga aagattgtat aatatgtaca gcggtaaact
    13381 gtacaatttc attatgcggc tttcttccgg caatcaatat atggctgaag aagtggtgca
    13441 atccactttt atccgtatat gggaagtacg tgagaaagtt gatacgaacg cttcatttat
    13501 ttcttttctt tgtacgatag ccaaaaattt actgatgaat atgtaccagc gtcagaccgt
    13561 tgaatatgtt tacaatgaat atctgctgaa aagcggacta gaccatgatt cacagacgga
    13621 agatactatt gatcttcgtt tcttgaacga ttatatcgac tcattagcgg aagaattgcc
    13681 ggcacagcga aagaaaatct ttatcttaag taagcgtcag aactatacga ataaggaaat
    13741 tgctgaaatg atgggtattt ctgaaagtac agtcgccacg caattatctt tggcggtgaa
    13801 atttatgcgt gaacagttga tgaagcatta cgataaggta attgcgcttc tgattgcctt
    13861 ttttgttaat gaaatgtaat tgtaatacac gtttttggat agtagatagg atgaaaatgt
    13921 atacttgtaa ataagcagga aagaagatgg ataagatata ttataaagaa ctgattgaaa
    13981 agtatttcga tggaaatata acggatgctg aaataaaaaa gctctccgat tggataaaga
    14041 acgaccgcca tttacagaac tggtgggaag aagaattttc caaatcggat gccggcatta
    14101 atccggtact gcgcgataaa ctatttgccc gaatcaaaga acagacgcag ggaaaagaag
    14161 aaacacaggg aaaagagaac ccaagaacta ttcgtatgaa tccttggaaa tgggctgccg
    14221 ccatcgtttt acccatttgt attgccttct tcacctacta tcttgtagat tcttcccaaa
    14281 cagtgggagc gccctttata gtaaaagctg ataaaggaga taaagcaacc atcgaattgc
    14341 cggacggaac gaatgtcgtt ctcaattctg cttcgcaatt aagctatctg aataacttcg
    14401 gagaaaatgg gcgtcgtgtg caactgaatg gtgaagccta tttcaaagta gctcatgatg
    14461 aaaagtgcgc ctttattgta caggtaggcg atttggaagt gaaagtactc ggtacttctt
    14521 ttaatgtatc tgcctatgaa gatgctaaag acgtgacagt cgttcttttg gaaggaaaag
    14581 ttggagtcta tgcgcagaaa acatcgcata tcatgaagcc cggcgacaaa atagaatata
    14641 ataaagccac tcataagata acagccactc aagtacaccc gagtgactac attgaatgga
    14701 cgaaaggaaa tatctatttc gagaaagaat ctttggagaa tataatgaaa accctttcac
    14761 gcatctatga cgtagagatt cgtttcgact ccaataaact acctaatgaa tatttcaccg
    14821 gaaccattcc gggaggtggc atacagaatg cattgaatat cctgatgctt acttcacctt
    14881 tctattatga aatggacggt tcggtgattg ttttgaaaga gaaataagtg gaactttcat
    14941 agagtatctt tgtttattca tcgaataa
//
