GCA_009389465.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaCHDEFGKN_77 (original name was: gnl|Prokka|CHDEFGKN_77)

Region	Type	From	To	Most similar known cluster		Similarity
Region 44.1	lanthipeptide-class-ii,RiPP-like	1	16,085

gnlProkkaCHDEFGKN_98 (original name was: gnl|Prokka|CHDEFGKN_98)

Region	Type	From	To	Most similar known cluster		Similarity
Region 65.1	NRPS	1	13,974

gnlProkkaCHDEFGKN_140 (original name was: gnl|Prokka|CHDEFGKN_140)

Region	Type	From	To	Most similar known cluster		Similarity
Region 107.1	T3PKS	1	4,476

Region	Type	From	To	Similarity
Region 44.1	lanthipeptide-class-ii,RiPP-like	1	16,085
Region 65.1	NRPS	1	13,974
Region 107.1	T3PKS	1	4,476

Compact view

gnlProkkaCHDEFGKN_77 - Region 1 - RiPP-like,lanthipeptide-class-ii

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Location: 1 - 16,085 nt. (total: 16,085 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg44_9

ctg44_7

Leader:

MSNKDLNNIVGG

Core:

AFEEMSIQQMTEIQGLGVTQVQSTPTVAISRAGIAASRASSVRCARWVSAAAGGAYTWKKHH

Core with dehydrations:

AFEEMDhaIQQMDhbEIQGLGVDhbQVQDhaDhbPDhbVAIDhaRAGIAADhaRADhaDhaVRCysARWVDhaAAAGGAYDhbWKKHH

MIBiG 3.1 matches for ctg44_7

8.1% BGC0000582 (ADR72962.1): Linaridin: cypemycin

23

STPTVA

28

+TPTVA

4

ATPTVA

9

antiSMASH-DB 4.0 matches for ctg44_7

40.3% NZ_CP045729.1: Lanthipeptides: allorf_1568662_1568871_lanthipeptide

11

TEIQGLGVTQVQSTPTVAISRAGIAASRASSVRCARWVSAAAGGAYTWKKHH

62

T IQG G Q++P + IS+A SSVRCA+WVSA +G +W KHH

1

TSIQGNGAESAQTSPLIPISKA-------SSVRCAKWVSAISG--LSWAKHH

43

30.6% NZ_CP045729.1: Lanthipeptides: LG045_RS07910_lanthipeptide

17

GVTQVQSTPTVAISRAGIAASRASSVRCARWVSAAAGGAYTWK

59

G QSTP AISR ++ +R SS +C W+S +AGG ++K

1

GTETAQSTP--AISRVTLSIARKSSAKCISWISFSAGGLNSYK

41

22.6% NZ_FPCC01000026.1: Lanthipeptides: BM377_RS12925_lanthipeptide

1

AFEEMSIQQMTEIQGLGVTQVQSTPTVAISRAGIAASRASSVRC

44

AFEE+ + M ++QG G + ++T +++ A A++ SS +C

1

AFEELDVDDMQQVQGAGDVEAETTVPLSVVIATAASAIYSSKKC

44

with 8 more sequence matches

22.6% NZ_KB976303.1and 1 other:

NZ_KB976303.1 Lanthipeptides: IGY_RS26035_lanthipeptide

Lanthipeptides: IGY_RS26045_lanthipeptide

25

PTVAISRAGIAASRASSVRCARWVSAAAG

53

PTV I SRASS C VSA +G

9

PTV------IVVSRASSKACMSGVSAISG

31

21.0% NZ_KN125582.1: Lanthipeptides: DJ91_RS27550_lanthipeptide

25

PTVAISRAGIAASRASSVRCARWVSAAAG

53

PTV I SR SS +CA +SA +G

9

PTV------IVVSRISSAKCASTISAISG

31

17.7% NZ_FPCC01000026.1: Lanthipeptides: BM377_RS12915_lanthipeptide

1

AFEEMSIQQMTEIQGLGVTQVQSTPTVAISRA

32

A+E+++ + M QG G T+ + T ++AI+ A

1

AYEDLNTEDMQNTQGAGDTEAE-TVSLAITGA

31

16.1% NZ_CP031746.1: Lanthipeptides: allorf_1813979_1814164_lanthipeptide

42

VRCARWVSAA---AGGAYTWKK

60

V CARWV + AG TW+

5

VGCARWVPCSLLGAGCPVTWRP

26

14.5% NZ_LT800494.1: Lanthipeptides: B7E07_RS02625_lanthipeptide

17

GVTQVQSTPTVAISRAGIAA

36

GV + Q+TPT+A++ A + A

2

GVAEPQATPTIALTSALVGA

21

12.9% NZ_CP094681.1: Lanthipeptides: K5I26_RS05295_lanthipeptide

20

QVQSTPTVAISRAGIAASRASSVRCARWVS

49

+VQ V GI R ++ CA +++

6

EVQEAQKVCCPDKGIERGRTTTSDCALFLT

35

12.9% NZ_FPCC01000026.1: Lanthipeptides: BM377_RS12905_lanthipeptide

1

AFEEMSIQQMTEIQGLGVTQVQSTPTV

27

+FE+++ + M QG G ++TP +

1

SFEDLNTEDMQNTQGAGDVDAETTPVI

27

12.9% NZ_PVMZ01000043.1and 1 other:

NZ_PVMZ01000043.1 Lanthipeptides: CLV67_RS49775_lanthipeptide

Lanthipeptides: CLV67_RS49775_lanthipeptide

39

ASSVRCARWVSAAAGG

54

+SS RCA ++ A GG

1

SSSTRCAESIARATGG

16

allorf_08469_08606

Leader:

MQLLHNLLHPFEKYSIYNA

Core:

YSPSSLCSIVIRKMFFLAFYIKRCSF

Core with dehydrations:

YDhaPDhaDhaLCysDhaIVIRKMFFLAFYIKRCysDhaF

allorf_13467_13619

Leader:

MATSQQEVALKGS

Core:

RERCNLSLQAIRVSQLTKLMCTRSKLLANANKCYNLK

Core with dehydrations:

RERCysNLDhaLQAIRVDhaQLDhbKLMCysDhbRDhaKLLANANKCysYNLK

antiSMASH-DB 4.0 matches for allorf_13467_13619

20.5% NZ_CP020327.1: Lanthipeptides: B5S25_RS25065_lanthipeptide

15

QLTKLMCTRSKLLANANKCYNL

36

+LTK++ + +L+ N K Y L

3

KLTKVLRRQHRLVINKKKVYRL

24

18.9% NZ_AP018268.1: Lanthipeptides: CDC39_RS06790_lanthipeptide

17

TKLMCTRSKLLANANK

32

T ++C R + NANK

7

TPIICLRYTMFRNANK

22

18.9% NZ_JALMDK010000007.1: Lanthipeptides: MZC64_RS28180_lanthipeptide

13

VSQLTKLMCTRSKLLA

28

S +T+L CTR+ L +

3

TSVITRLACTRTPLCS

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg44_7 - Class II

Cleavage pHMM score: -0.90

RODEO score: 0

Monoisotopic mass: 6370.3 Da

Molecular weight: 6374.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 6392.2 Da N = 1; 6410.2 Da N = 2; 6428.2 Da N = 3; 6446.2 Da N = 4; 6464.3 Da N = 5; 6482.3 Da N = 6; 6500.3 Da N = 7; 6518.3 Da N = 8; 6536.3 Da N = 9; 6554.4 Da N = 10; 6572.4 Da N = 11

allorf_08469_08606 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 3015.6 Da

Molecular weight: 3017.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3035.7 Da N = 1; 3053.7 Da N = 2; 3071.7 Da N = 3

allorf_13467_13619 - Class II

Cleavage pHMM score: -7.10

RODEO score: 0

Monoisotopic mass: 4189.3 Da

Molecular weight: 4192.0 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 4210.0 Da N = 1; 4228.0 Da N = 2

gnlProkkaCHDEFGKN_98 - Region 1 - NRPS

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Location: 1 - 13,974 nt. (total: 13,974 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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transport-related genes

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 13974:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (X) + (Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg65_2

Search NORINE for peptide: strict or relaxed

AMP-binding (7..393): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg65_3

AMP-binding (267..640): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVRHFALLAK
Nearest Stachelhaus code(s):: Asp DVRHFALMAK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg65_4

Search NORINE for peptide: strict or relaxed

AMP-binding (484..878): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaCHDEFGKN_140 - Region 1 - T3PKS

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Location: 1 - 4,476 nt. (total: 4,476 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.