LOCUS       gnlProkkaPFOAHMEE_152   5504 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PFOAHMEE_152.
ACCESSION   gnlProkkaPFOAHMEE_152
VERSION     gnlProkkaPFOAHMEE_152
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 00:37:32
            Original ID  :: gnl|Prokka|PFOAHMEE_152
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5504
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5504
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[130:3842]"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      131..3842
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     cand_cluster    1..5504
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5504
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="betalactone"
                     /region_number="1"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     CDS             complement(6..134)
                     /locus_tag="ctg152_1"
                     /transl_table=11
                     /translation="MISDCFYVFKSFSPAIRPEESFFVLHLSAGNKLESKWERGTG"
     CDS             complement(131..1708)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg152_2"
                     /sec_met_domain="HMGL-like (E-value: 1.3e-31, bitscore:
                     98.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRKLLIRDLTLRDGQQSVFATRMSQKQIDKVLPFYREAGFYAME
                     IWGGAVPDSIMRYLGEDPWERLEKIKASVGNTSKLTALSRGRNLFGYNPYPDKIIKGFC
                     RNSIESGLDIMRIFDALNDVENIKSTVKYVKRFGGMADCAVCYTIDPYHSAVERIVAAL
                     HGHPLHKPVFTNEYFLDKALQMEALGADMITIKDMSGLIPPGRSAEIVRLFKKHLKVPV
                     DFHTHCTPGYGLASVLAAIVNGVDVVDTNIWYFAGGTAAPAIELVYVFCKKMGIELDIN
                     MEAIAKINAHLLDIRKELSVFDMAKQLPKPFNPLTDRIPTEIDRFFNDAIDAARKDKEE
                     DLLIFCRAIEEYFGFPEPNELVKKAQIPGGMYTNMVAQLKQLGQLDLLEKAMSLIPQVR
                     MDAGLPPLVTPTSQIIGAQAVSCALDQLKGRPMYSNPSNQFVALVKGEYGKTPVPIDPE
                     FRLKITGSRDEVPYDPSDYEMQENPVIEEVGVQLAENEKEVLLLELFPMMAHNFLAKKK
                     QKQVHVAT"
     CDS             complement(1822..2091)
                     /locus_tag="ctg152_3"
                     /transl_table=11
                     /translation="MKTSYFLGKNNLKSFFRPGWKGMGDPGVEEVETRIFVKPERGCGA
                     VYLPCLRRFYALFLIIIQWITLCILPRHDSFLLYWKENVKKISR"
     CDS             2541..3842
                     /NRPS_PKS="Domain: AMP-binding (70-286). E-value: 2.6e-22.
                     Score: 70.8. Matches aSDomain:
                     nrpspksdomains_ctg152_4_AMP-binding.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score: 76;
                     E-value: 3.5e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg152_4"
                     /sec_met_domain="AMP-binding (E-value: 3.3e-27, bitscore:
                     83.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYWQKDIETMNREDLKKLQFERLRKTIEAAGNSPFYSKVFKENG
                     ITADSIQSLDDLQKIPFTTKDDLRNNYPYGMAAIPIKDCVRIHSSSGTTGNPTVVLHSA
                     RDLDQWANQVARCMYMVGIRDTDVFQNTSGYGMFTGGLGFQYGAEKLGCLTVPAAAGNT
                     KRQIKFITDFGTTCLHIIPSYATRLAEVMYEMGLDPRRDTKLHTVCIGAEPHSEEQRRR
                     IEQLLGVKAYNCFGMSEMNGPGVAFECTEQNGLHIWEDNVIVEIIDPVTLQPVKEGEVG
                     EMVLTTINREAMPLLRYRTRDLTCVLPGECPCGRTHKRLARFKGRSDDMIILKGVNLFP
                     IQIEKILMQFKELGSNYLITIETVGNNDEMLIEVELSDLFTDDYSVLQRLTKEITRQLK
                     DELLLTPHLKLVAKGSLPVQDGKAVRVKDLRKLC"
     aSDomain        2751..3398
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg152_4_AMP-binding.1"
                     /evalue="2.60E-22"
                     /label="ctg152_4_AMP-binding.1"
                     /locus_tag="ctg152_4"
                     /protein_end="286"
                     /protein_start="70"
                     /score="70.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="NYPYGMAAIPIKDCVRIHSSSGTTGNPTVVLHSARDLDQWANQVA
                     RCMYMVGIRDTDVFQNTSGYGMFTGGLGFQYGAEKLGCLTVPAAAGNTKRQIKFITDFG
                     TTCLHIIPSYATRLAEVMYEMGLDPRRDTKLHTVCIGAEPHSEEQRRRIEQLLGVKAYN
                     CFGMSEMNGPGVAFECTEQNGLHIWEDNVIVEIIDPVTLQPVKEGEVGEMVLT"
     CDS             3875..4666
                     /locus_tag="ctg152_5"
                     /transl_table=11
                     /translation="MRIIPKANTLRKGILLYRGIRYRKGFGVHSPFVFNLITKVIEERC
                     QYYSFYDIELIRKQLLFQDDEITYPDRQQKGKMRRRTIGRIVEREAIRPKHGALLFRLA
                     NYFKSKHILQFGPSMGLSTLYLTSYASDLKCIALENVPEFASIARIAFGKAARNPVDLR
                     VGSYKDLLPKALKDMEQLDFVFFNTLYEQQNNNWLFEECLKHVHGDTVFVFEGIKTSRK
                     MREFWREVCCHPEVTVTIDLYSMGIVFFNKKLHKRDYIVYF"
     CDS             5268..5504
                     /locus_tag="ctg152_6"
                     /transl_table=11
                     /translation="MKKSIIYTGTGDKGTTSLVGGERVSKAHQRIESYGTVDELNSFIG
                     LLITSLEEKADQDFLLFIQHKLFTIGSYLATDQE"
ORIGIN
        1 cagaattatc ctgtacctct ttcccatttc gactccagct tatttccggc agacaaatgt
       61 aaaacaaaaa agctttcctc cggacgaata gccggggaaa agctcttaaa tacataaaaa
      121 caatctgata tcatgtggca acatgcactt gtttctgctt cttcttcgcc aggaaattat
      181 gcgccatcat cgggaaaagc tccagaagca agacctcttt ttcgttctca gccaattgga
      241 caccaacttc ttctattacg ggattctcct gcatttcata atcggaagga tcatagggga
      301 cctcatcccg cgaacccgta atcttcaaac gaaattccgg atcaatcgga accggcgtct
      361 ttccatactc tccttttacc agagcgacga attggttgga cggattactg tacatcggac
      421 gtcctttcag ttgatccaac gcgcacgata ctgcttgagc cccgatgatc tggcttgtcg
      481 gcgttaccaa aggcggcaaa cctgcatcca tacgcacctg ggggatcaac gacattgctt
      541 tttccaacaa gtccaactgt cctaattgct tcaactgagc taccatattc gtatacatac
      601 ctcccggtat ctgggccttc ttcaccagtt cattcggttc agggaaacca aaatattctt
      661 ctatggcacg gcagaagata agcaaatctt cctctttgtc tttccgggct gcgtcgatcg
      721 catcgttaaa aaaacggtcg atctcagtcg gtattctgtc tgtcaaaggg ttaaatggct
      781 taggtagttg ttttgccata tcgaaaacag acaactcttt gcggatatcc aacagatgag
      841 cattgatttt cgcgatcgcc tccatattga tatccagctc gatccccatt ttcttacaga
      901 acacgtacac cagttcaatt gccggagcag ccgttccacc ggcgaaatac cagatattgg
      961 tatcgacaac atccaccccg ttgacaatag cggccaaaac cgaagccaga ccatatcctg
     1021 gcgtacaatg agtatgaaaa tctaccggaa ctttgagatg ttttttgaac aaacgtacta
     1081 tttcagcgct tcttcccggt ggaatcaggc cactcatatc cttaatcgtg atcatatcgg
     1141 ctcctaaagc ctccatttga agcgctttat ccaagaagta ttcatttgta aaaacaggct
     1201 tatgcaacgg atgcccgtgt aaagctgcga caattctttc aacagccgag tgatacggat
     1261 caatcgtgta acagacggca caatcagcca ttcctccgaa gcgttttaca tatttgactg
     1321 tcgattttat attctccaca tcattcagcg catcaaatat gcgcatgata tccaatccgg
     1381 attctataga attgcgacag aagcctttaa taatcttatc gggataagga ttgtaaccaa
     1441 acaagttacg tccgcgtgaa agggccgtca acttagaggt attccctacc gaagctttta
     1501 ttttctccaa ccgctcccaa ggatcttctc ccaaatatcg catgatagaa tcgggcacgg
     1561 caccacccca tatttccatc gcataaaaac cggcttccct ataaaaagga agcactttgt
     1621 ctatctgttt ttgtgacatt cgcgttgcaa aaacagattg ctgaccatcc cggagagtca
     1681 gatcccgaat caaaagtttt cgtatcatac ctaataattt gtttagaccg tttcagactt
     1741 aaatctatct ttataacaaa aattaaactg ttttgattgg aattaccggg ttaagaatag
     1801 caaatgttta tgaaaacgac cttaccgact tatcttttta acattctctt tccaataaag
     1861 aaggaagcta tcatgccgtg ggaggataca caaggtaatc cactggatta taatcagaaa
     1921 cagtgcataa aatctcctca aacaaggcag ataaacagct ccacatcccc gttccggttt
     1981 cacgaagatc cgggtctcca cctcctccac acccggatct cccatgccct tccacccggg
     2041 tctgaagaaa cttttcaagt tgtttttgcc taaaaagtag cttgtcttca tgaaaaaacg
     2101 agcctcttta atctattttg tatcagcttc agaacatatc tgaaatttga taaaatattg
     2161 atatacaatg ctttattgct tttacccatg atggttatga tagatgaatg atagttgaaa
     2221 acaggaacta tcatagaata acatactgtt ttacaagcat ataaattcaa ttatgatagt
     2281 atgatagttg ttttgcacaa aacgcaagtt ctttgttacc ctgccttatt gaacattgtc
     2341 cttttgatag tttttactct gtttaccgtc ccttcgagta aggcattact gtagtttgtg
     2401 tcaacagcca cctgtaccgc tgccttgtcg acctctatgt tatatacgaa tctgaccggg
     2461 ctgatctttg atttctccaa aattaacaat acatttgcag tctaatttca tgaatcaaac
     2521 acattataat gcaaaaaacg atggaatatt ggcagaaaga tattgaaacc atgaatcgcg
     2581 aagatttgaa aaagctacag tttgagcgat tgagaaagac aattgaagca gccggaaatt
     2641 ctcctttcta tagcaaagtt ttcaaagaaa acggcatcac agccgacagt atacaatcac
     2701 tggacgattt gcaaaagatt ccttttacga cgaaagatga ccttcgcaac aattatccgt
     2761 acggcatggc ggctattcct atcaaggact gtgtccgtat ccattcgtca agcggtacta
     2821 caggaaaccc aacagtcgta ttgcattcgg ccagagactt ggaccaatgg gctaaccagg
     2881 tggcacgctg tatgtatatg gtaggcattc gcgacacaga cgtattccag aacacttccg
     2941 gttacggaat gtttaccgga ggattgggat tccaatacgg agctgaaaaa ttaggttgcc
     3001 tgaccgtacc ggcagcagca ggaaatacca agcggcagat caagttcatt acggatttcg
     3061 ggacgacttg cctccatatc attcccagct atgctactcg cctggcggaa gtgatgtatg
     3121 aaatgggcct cgatccccgt cgcgacacca aactgcacac cgtttgtatc ggtgctgagc
     3181 ctcactcgga agaacaacgc cgccggatcg aacaattgtt aggcgtaaaa gcatacaatt
     3241 gtttcggaat gtcagagatg aacggccccg gcgtcgcatt cgaatgtacg gaacagaacg
     3301 ggctccatat ctgggaagat aatgtaatcg tagaaattat cgatccagtc acactgcaac
     3361 cggtaaaaga aggtgaagtc ggcgaaatgg tcctgacgac catcaaccga gaggcaatgc
     3421 ctttgctccg ttatcgtacc cgcgacctga cctgtgtcct tccgggtgaa tgtccctgcg
     3481 gacgaacgca caaacgcctt gcacgtttca aaggccgtag cgacgacatg atcatattga
     3541 aaggggtcaa cctcttccct atccagatcg aaaagatact gatgcagttt aaagaactgg
     3601 gtagcaatta tctaattaca atcgaaacgg taggaaacaa tgacgagatg ctgattgaag
     3661 tggaattgag cgacctgttt accgacgatt acagcgtttt acaacgcttg acaaaggaaa
     3721 tcacccgcca gctgaaagac gagctgcttc tgactcccca tctcaaactg gtcgccaaag
     3781 gctccctgcc ggtacaggat ggaaaagctg tccgggtgaa agacttgcgt aaactttgct
     3841 gaactcataa ttcataaatc taaaatcata aatcatgcgc attatcccca aagctaatac
     3901 actaagaaaa ggcatacttt tatacagagg catccgttac cgcaaaggct tcggggttca
     3961 ttcccccttt gtcttcaacc tgatcactaa agtgattgag gagcgttgcc aatactatag
     4021 cttctacgac atcgaactga tccgcaagca gctgcttttc caagatgacg agatcactta
     4081 tcccgaccgc cagcaaaagg ggaagatgcg ccgccgcacg atcggccgga ttgtcgaacg
     4141 cgaagcgata cggcccaagc acggcgcctt acttttccgt cttgccaact atttcaaatc
     4201 aaaacacatc cttcagttcg gaccttccat gggcctgtcc actttgtatc tgacttcata
     4261 tgcatccgat ctgaaatgta tcgctctgga aaacgtaccg gagtttgcct ccatcgcccg
     4321 tatcgctttc ggcaaagccg cccgtaaccc ggtcgacctg cgtgtcggaa gctacaaaga
     4381 tttactaccg aaagccttga aagacatgga gcaactcgat tttgtctttt tcaacaccct
     4441 gtatgagcag caaaacaata actggctgtt tgaggaatgc ttgaaacatg tgcacggcga
     4501 tacggttttt gtcttcgaag gcatcaaaac cagccgcaaa atgcgcgaat tctggcggga
     4561 agtatgctgt catcccgaag taacggtgac gatcgacctg tattcaatgg gaatcgtgtt
     4621 tttcaacaag aagttacaca agagggatta tatcgtctat ttctgactct ttatccaaag
     4681 atacaaacaa attgatcttt gacttataca tccgtctatc acctcatcga ctgcaatgct
     4741 cgatttgatc tgtgaccgtg ataataatag gaaacaataa caaatatgca aataaaaata
     4801 tacagaccat acgctaactt gaataaaagt attatagagt gatagaggcc atacttctag
     4861 ctttaaacta taaggtcata gagtttttga cctaagtcga ataccttttc gaccttagtg
     4921 aaaaaggttt tcgaccatag gcaaaaaggt atttgactta ggccgaaaat aaaaaaggat
     4981 ataggacaaa acgtaaatat ctatatatta gcacacaaaa gttttccgca atctcctcat
     5041 ttatcactta aatatgtcgc tttgggtaaa aggacgaggg ggaggaacta taatcaagct
     5101 ggatcaagac gagacattgg taaagatgcc tgcaattttt tctttcccat caaaataaat
     5161 aatccgaatc cttgcacaaa tcccggaatc tgacgaatat tgtactcctt tttgcaacat
     5221 ctggcaagga tgtgcgatta tataataaaa cgataagtta ttgtcatatg aaaaagagca
     5281 ttatatacac agggaccggc gacaagggaa ccacttcctt agttggcggc gaacgggtat
     5341 cgaaagcgca ccaacgtatc gaaagctatg gtacggtcga cgaactgaac tcgtttatcg
     5401 ggttgttgat cacttcgctt gaagagaaag cggatcagga cttcctgctg tttatccagc
     5461 acaagttgtt tacgatcggt tcctatctgg caaccgatca ggaa
//
