LOCUS       c00375_gnlPro..         1652 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HACBHPDJ_375.
ACCESSION   c00375_gnlPro..
VERSION     c00375_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 09:32:28
            Original ID  :: gnl|Prokka|HACBHPDJ_375
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1652
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..552)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 70; E-value:
                     8e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_1"
                     /sec_met_domain="Radical_SAM (E-value: 2e-29, bitscore:
                     90.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VERMSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVAFMQ
                     GCHMRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFA
                     LELFKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINPDEHKRLT
                     GFPNDNILDMCEYLSSIGKPV"
     protocluster    1..1652
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1652]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1652
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1652
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1652
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(570..1652)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_2"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-42, bitscore:
                     131.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.4e-91, bitscore:
                     294.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="STIQYENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLA
                     KAVLYAINGGKDELTKQQVGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIH
                     YMHDKYYYESAQMALKDTLVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDF
                     KADHDFPRYGNDDDRADDIAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNT
                     GTTPNGRQKGEPFAPGANPAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLG
                     HDEKMQEDTLVNMIGGYMRKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYF
                     ADLTKEQQDDVIARTFHASM"
ORIGIN
        1 aacaggtttc cctatgctag aaagatattc gcacatatcc aaaatattat catttgggaa
       61 tccggttaat ctcttatgtt cgtccggatt gatatgcttg atatcaacta ataaaatgtc
      121 ggtatagtcc attaactcat tgaacttact aaaccaaggt tctcttcttg taaatggctg
      181 tccacaagtg tccaaacaag tgctaatacc ttcttccttg cacatcttga ataactctag
      241 tgcaaaatct atttgcaata gaatttcacc acctgataaa gttattccac ctttgtcgcc
      301 ccaaaagctg cgataaggaa gtgccttgtt tagaacttct tcagtcgtca tttgtgaacc
      361 tacccttgtt ttccaagtgt ccggattgtg acagaactta cacctcatat ggcaaccttg
      421 cataaaagcg acaaaacgta tacctggtcc atcaacagct ccaaaagttt ccaaagaatg
      481 tacatatcct acgacatgac cgtctttagt tggaactgga ttcaataaag gattagcact
      541 cattctctcc acctactctc ttctaattat tacattgaag catggaaggt tcttgcaata
      601 acgtcatctt gttgttcttt agttaaatca gcaaagtata cacagtaacc tgaaacacga
      661 attgttaatg ttggatactt ctctggatgt gcttgagcat ctaataaagt atctctgttg
      721 aatacattaa tattcaaatg atgaccatct tttctcatat agcctccgat catgtttacc
      781 aatgtatctt cttgcatctt ttcatcatga cccaatgttg ttggtgttac agcaaatgta
      841 ttagaaatac catctgtagc atacttgtat ggtaatttag caacggataa tagtgatgct
      901 aaagcacctg ttgtatctgc tccgtatgct gggttagcac ctggtgcaaa tggttcaccc
      961 ttttgacgac catttggagt agtacctgtg ttcttaccat aaacaacatt tgaagtaatt
     1021 gtaagaacgg atgttgttaa cttagctcca cgatatagat gatgcttgct catcttagta
     1081 aatagttcct taacaagcca tgcagcaata tcgtcagcac gatcatcatc gttaccataa
     1141 cgagggaagt catgatctgc cttgaagtca acagcaatac catcttcgtc acgaattact
     1201 ttaacatgtc cgtacttgat agctgaaatt gagtcagcag caacagacaa tccagagata
     1261 cctgtagcaa agctacgatc tactaatgta tctttcaatg ccatttgtgc tgattcatag
     1321 taatacttgt catgcatgta atgaattgtg ttcaaagcat ttacataaat atcagcaatc
     1381 cattccatca tgtcatcgaa tttcttcatg aattcatcgt agtcaatgta ttctgagcta
     1441 attggttcgt atgcaggacc tacttgttgt tttgtaagtt catccttacc accattgata
     1501 gcatacaata cagccttagc tagatttgca cgagctccga agaattgcat accatttgca
     1561 attggttgtg cagatacaca gcaagcaatg ccatagtagt ctgttcccca tgtcttcatc
     1621 attaagtcat cattttcgta ttgaattgta ga
//
