LOCUS       c00303_gnlPro..         2203 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BBECOPNO_303.
ACCESSION   c00303_gnlPro..
VERSION     c00303_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 10:14:14
            Original ID  :: gnl|Prokka|BBECOPNO_303
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2203
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2203
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2080]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2080
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2203
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2203
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1217
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg303_1"
                     /sec_met_domain="Gly_radical (E-value: 9.9e-42, bitscore:
                     129.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-114, bitscore:
                     371.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTI
                     QYENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQ
                     QVGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKD
                     TLVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRAD
                     DIAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFGVVP
                     GANPAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIG
                     GYMRKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIART
                     FHASM"
     CDS             1244..2080
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.4;
                     E-value: 2.6e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg303_2"
                     /sec_met_domain="Radical_SAM (E-value: 8.2e-35, bitscore:
                     107.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVAFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINPDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYQEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
ORIGIN
        1 catcatatcg tttcttaaac acattgcata atatgggtgc cgcaccagaa ccaaatatca
       61 cattactttg gtctgatcgt ttgcctgaat catttaagca ctatgctgca agtgtatcta
      121 ttgcaagttc tacaattcaa tacgaaaatg atgacttaat gatgaagaca tggggaacag
      181 actactatgg cattgcttgc tgtgtatctg cacaaccaat tgcaaatggt atgcaattct
      241 tcggagctcg tgcaaatcta gctaaggctg tattgtatgc tatcaatggt ggtaaggatg
      301 aacttacaaa acaacaagta ggtcctgcat acgaaccaat tagctcagaa tacattgact
      361 acgatgaatt catgaagaaa ttcgatgaca tgatggaatg gattgctgat atttatgtaa
      421 atgctttgaa cacaattcat tacatgcatg acaagtatta ctatgaatca gcacaaatgg
      481 cattgaaaga tacattagta gatcgtagct ttgctacagg tatctctgga ttgtctgttg
      541 ctgctgactc aatttcagct atcaagtacg gacatgttaa agtaattcgt gacgaagatg
      601 gtattgctgt tgacttcaag gcagatcatg acttccctcg ttatggtaac gatgatgatc
      661 gtgctgacga tattgctgca tggcttgtta aggaactatt tactaagatg agcaagcatc
      721 atctatatcg tggagctaag ttaacaacat ccgttcttac aattacttca aatgttgttt
      781 atggtaagaa cacaggtact actccaaatg gtcgtcaaaa gggtgaacca tttggagtag
      841 taccaggtgc taacccagca tacggagcag atacaacagg tgctttagca tcactattat
      901 ccgttgctaa attaccatac aagtatgcta cagatggtat ttctaataca tttgctgtaa
      961 caccaacaac attgggtcat gatgaaaaga tgcaagaaga tacattggta aacatgatcg
     1021 gaggctatat gagaaaagat ggtcatcatt tgaatattaa tgtattcaac agagatactt
     1081 tattagatgc tcaagcacat ccagagaagt atccaacatt aacaattcgt gtttcaggtt
     1141 actgtgtata ctttgctgat ttaactaaag aacaacaaga tgacgttatt gcaagaacct
     1201 tccatgcttc aatgtaataa ttagaagaga gtaggtggag agaatgagtg ctaatccttt
     1261 attgaatcca gttccaacta aagacggtca tgtcgtagga tatgtacatt ctttggaaac
     1321 ttttggagct gttgatggac caggtatacg ttttgtcgct tttatgcaag gttgccatat
     1381 gaggtgtaag ttctgtcaca atccggacac ttggaaaaca agggtaggtt cacaaatgac
     1441 gactgaagaa gttctaaaca aggcacttcc ttatcgcagc ttttggggcg acaaaggtgg
     1501 aataacttta tcaggtggtg aaattctatt gcaaatagat tttgcactag agttattcaa
     1561 gatgtgcaag gaagaaggta ttagcacttg tttggacact tgtggacagc catttacaag
     1621 aagagaacct tggtttagta agttcaatga gttaatggac tataccgaca ttttattagt
     1681 tgatatcaag catatcaatc cggacgaaca taagagatta accggattcc caaatgataa
     1741 tattttggat atgtgcgaat atctttctag catagggaaa cctgtttgga ttcgtcatgt
     1801 ccttatccca ggcatcacgg ataatgatga atacttgaaa caattgggcg agtacgttaa
     1861 ggaaaatctt cataatgtcg aaaaatttga agtattgcct tatcatacaa tgggcgttca
     1921 taagtatcag gaaatgggta tcagatatcg tcttgaaggt gttgagcctc ctacgccaga
     1981 aagagtaaag aatgctgaaa aactgctaga ggtggataga tatcaaggat ataaagattg
     2041 gtatccaggc attaatactt tggatcaaca accaagctag ctaaattggc aaatataaaa
     2101 caaaacatgg tatcgcttag ttttacggtt cttttcaaaa tcctgtggat tcctaatatt
     2161 gaatccatag gattttttat tttctatgtg aaaaaaatta aaa
//
