LOCUS       c00674_gnlPro..         2646 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LIOKIPGN_675.
ACCESSION   c00674_gnlPro..
VERSION     c00674_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 04:42:19
            Original ID  :: gnl|Prokka|LIOKIPGN_675
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2646
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2646
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1571]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1571
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2646
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2646
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..832
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg674_1"
                     /sec_met_domain="Gly_radical (E-value: 4.6e-47, bitscore:
                     147.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-55, bitscore:
                     177.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRAT
                     GIAGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEK
                     FMEYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTK
                     GAVAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQVNNLVSLLDGYFTPDGGQHL
                     NVNVFDKDLLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             849..1571
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg674_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.8e-36, bitscore:
                     113.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCSLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFSKHGITV"
     CDS             complement(1574..1879)
                     /locus_tag="ctg674_3"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             2155..2646
                     /locus_tag="ctg674_4"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKIAEIHDNGIGRNADELLRKVEAAQFVASHP
                     K"
ORIGIN
        1 cgagcagatg atgcgctggt tggcaaaggt gtatgtcaat gcactgaaga tcattcacta
       61 catgcacgac aaatatgcgt atgaagcatt cgagatgggg cttcatgacg gagatgtgga
      121 acgtatccgc gcaaccggta tcgccggcct ttctatcgtt gccgactcgc tggctgccat
      181 ccgtgacacg aaagtgaaag tgatccgtga cgaacgtggt ttggctgtcg acttcgaacg
      241 cgaaggcgaa tatgtacctt tcggaaacaa tgacgaccgc acggacagta ttgctgtcga
      301 cattactgag aagtttatgg aatatctgcg ccagcatcag acataccgca aggcaactcc
      361 gacacaatct atcctgacga tcacatcaaa cgtggtctac ggaaagaaaa ccggaacgac
      421 tcccgacggc cgtccgggcg gtacgccgtt tgctccggga gccaacccga tgaacggacg
      481 cgatacgaaa ggggctgtcg ccgctttggc ttctgtcgcc aaactgcctt ttcagcatgc
      541 gcatgacggt atttcctata cattcgccgt ttctcctgca acgttgggta aggaacgcga
      601 tatccaggtg aacaacttag tgagcctctt agatggttat ttcacgcccg atggcggcca
      661 gcatctgaat gtgaacgtat tcgataaaga cttgctgtta gatgcgatgg aacatccgga
      721 gaaatacccg cagcttacga tccgtgtttc gggctatgcc gtcaacttcg ttaagctgac
      781 acgcgaacag cagctcgacg tcatctcgcg caccatcaac agcagtttat aattgtccat
      841 tgattaatat gttggggcgt atacattctt tagagagttt cgggaccgtt gacggtcccg
      901 gaattcgctt tgttgttttc atgcagggat gttcgcttcg ttgtctgtat tgccacaatc
      961 cggatacctg ggaggtaaag cgtgaaacgc cttatctgct ggaacctgaa gcgttgcttg
     1021 ccgaagtgct acgctataag aattttatcg ctaaaggtgg tgttactgtg accggtggcg
     1081 agcctttgtt acaacctgaa ttccttaaag agtttttccg cctttgccgt gagaacggta
     1141 ttcatacggc gttggacact tccggctata tctgttccgg taaagcattg gaggtgctcg
     1201 aacaggtcga tctggttctg ttagatatca agacgatcga cgccggtctt catccccgtt
     1261 tgacggccgt aaaactcgat aatactttac gctttcttga tgaactggag aaacgcggca
     1321 tccctgtatg gattcgccac gtgatcgtgc ccggcctgac ggacgatgat gaagccttga
     1381 gcaagctggc cgaatatatc agttcatata acgtggtcca gaaagccgaa cttctgcctt
     1441 atcatacaat gggagcttac aaatatgagg cacaaggcct ggactataag ctgaaaggtg
     1501 tggaaccgct ttcagcagaa cgtctggcca atgctaaagc gatctttagt aaacacggga
     1561 taaccgtata agcctaatcg agtgtctgct ctttgatgat gatcggttta cggctgatcc
     1621 ggttgactgt gcggacgctg tcgctttcaa tactggtaac atctccctcg cagttggtga
     1681 aatagatgta atgtcccata tccatgaaac gatagacctt acacccttcg tgttcgaaca
     1741 gatattcgac ttgataagtg gaattgttta ccggtttttc ctttgtaagg ggtatttgtg
     1801 agacacatga agaaagacat gcgttgcaaa cgagcaatag acttgctgaa aatgtattat
     1861 acaaactagc tcttctcatc ttcgaatatt tttaagttaa catccaaagt taacaattaa
     1921 agaatcacat ggtatgatcg aaaggtattt tacctacaat taggtatatt tttattgttc
     1981 cggaaaacat tactttgctt cctattgtca tagtagataa gtatgcttgc agatagaaat
     2041 ccgtctatcg gcagataaaa attatcaatt tgaaaaattc tttcaatcag tatcttacga
     2101 tttacctttg tagggtaaac aaaaatagta gtaacaaatt aaatagtaaa aatcatggaa
     2161 ccaattatca attcacaagt acccgaattc aaagttcaag cttttcagaa tggtagtttc
     2221 aagacagtaa gtagcgaaga tatcaagggc aaatgggcca tctttttctt ctatcctgcc
     2281 gactttacgt tcgtatgtcc gaccgaattg gtcgacatgg ctgacaagta cggcaaattt
     2341 caggaaatgg gtgtcgaagt atattccgta agtacagact cgcactttgt acacaaagca
     2401 tggcatgatg cttccgaaac gatccgtaag atcaaatatc cgatgctggc agacccgaca
     2461 ggggctttaa gccgtgcatt cggtgtgatg attgaagaag agggtatggc ttatcgcggt
     2521 acgttcctgg taaatcccga gggaaagatc aagattgccg aaatccacga taacggtatc
     2581 ggccgtaatg cagacgaatt gcttcgtaaa gttgaagctg cccagttcgt tgcttctcac
     2641 ccgaaa
//
