LOCUS       c00288_gnlPro..         2601 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KDEKJMMO_288.
ACCESSION   c00288_gnlPro..
VERSION     c00288_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 03:26:41
            Original ID  :: gnl|Prokka|KDEKJMMO_288
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2601
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2601
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1707]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1707
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2601
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2601
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..911
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_1"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-47, bitscore:
                     147.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 3e-62, bitscore:
                     198.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ELKGKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNI
                     IHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIV
                     DFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGK
                     KTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQA
                     LGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAV
                     NFIKLTREQQQEVINRTFHTR"
     CDS             928..1707
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_2"
                     /sec_met_domain="Radical_SAM (E-value: 2e-32, bitscore:
                     100.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHNPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(1956..2600)
                     /locus_tag="ctg288_3"
                     /transl_table=11
                     /translation="SGRDTHPGARKYKFRVKAPVLRAAPPAHRVQLYLQLGVLHGLDVA
                     TPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSREQWRELVSLLPGRTVLAALEEDRER
                     AASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDFPSLCSFRGLPVVTLFSTRLPDV
                     YRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALREISEKE"
ORIGIN
        1 atgaactcaa gggcaagcag gtggccccgc cctccccccg ctacacggag gaaattctca
       61 actatgatga agtgatggcc ctgtacgaca agatgcagga ctggctcgcc aaaacttaca
      121 ttgacgcgct gaacatcatc cattacatgc acgacaaata ctgctatgaa cgcattgaaa
      181 tggcgctgca tgatccggaa attctgcgca cgatggctac gggaatcgcc gggctttccg
      241 tggcggcgga ctccctgtcc gccatcaagt atgccaccgt aaaagccatc cgcaatgagg
      301 aagggctgat tgtggacttc aaaacggaag gggaattccc ctgttacggg aacaatgacc
      361 cgcgcgtgga cgacatagca tgcagcctgg tcagcaattt catggaaaaa ctgcgcaggc
      421 tccacactta ccgcaattcc ctgcccaccc agtccatcct gacgatcacc tccaacgtgg
      481 tgtacggcaa aaagacgggc aacacgccgg acggccgccg agccggggaa ccgttcgccc
      541 ccggagccaa ccccatgcac ggaagggaca ggaacggagc cgtggcctcc atgctctccg
      601 tagccaagct gtcctatgac gactccctgg acggcatctc ctacaccttc tccatcgttc
      661 ctcaggccct gggcaaggag gaacgtgaac gccgcgtcaa gctcgtctcc ctgctggacg
      721 cctactttgc cgctacaggt caccacatta atgtgaacgt actggaacgg gaaacgcttc
      781 tcgacgccat ggatcacccg gaaaaatacc cgcagcttac catccgcgtt tccggctatg
      841 ccgtgaattt catcaagctg acccgggaac agcagcagga ggtcatcaac cgcactttcc
      901 acacccgtta acccggactg caagcggatg ccgcaggttt ttccacaaaa ccataatccg
      961 gacggagaaa gctccgtcac cggtttggtt cattccgtgg aatcctgcgg caccgtggac
     1021 ggccccggca tccgcttcgt ccttttcctg tccggctgca gcctgcgctg ccgctactgc
     1081 cataatccgg atacctccta cgtgcggagg ggccggctca ggagcgcaga tgatgtcctc
     1141 aaggaaatag cccgttaccg ggattttttg caggcggcgg gcggcggagt caccctctcc
     1201 ggcggggatc ccctcttcca gccggatttt gccggtgccg tgctgaaagg ctgccggaaa
     1261 ctgggcctgc acacctgcct ggacacctcc ggccatctgg gcgtgaatgc gggcgaagaa
     1321 atgctggcgg atacggacct ggtcctgctg gatatcaagg catggaatcc gaaacggtat
     1381 cgcgccctga caggcgggga actgcgcccc acactggagt ttgcggaacg cctggcctcc
     1441 ctccgcaagc ccgtgtggct taggtatgtg ctggtacctg gcgttacgga caatctggaa
     1501 gaaattgagg aactctcccg ccatgcggcg cttctgggca acgtggaacg cgtggacgtg
     1561 ctccctttcc accagctggg gagattcaaa tgggatgaat tgaagctgga ctataccttg
     1621 aaggacgtcc cggaagcgtc cgcagaactg gcagaacagg tccgcggcat tttccggcgg
     1681 ggaggctttc cggattgcgt gcattaaccg gattttcgcg ctttggcgcc accaaccgca
     1741 gcaaaccgga cgcaggcatc cccctcttcc ggatgagggg ccctctcccc tccactgcag
     1801 aatccatgca aaaaggaaga cgcatttctg gaattgctac ggaatacgga atttccggaa
     1861 ttcctttcgt tatttcatca ttctttacgg caacggcagc attcccgccg ttttttccgc
     1921 caacgccggg ggacggcatt ccgtcccttc ccagattatt ctttctcgga aatttcccgc
     1981 aaggcttcca gtacgtcccg cacggcaatg tggcggttgc agggaacatc ccggtcacat
     2041 tccttccgga agcagggaga acagcactgg tggctgtaaa gggaacggtt gaatgtgccc
     2101 atagggcggt aaacatccgg aagccgggtg gaaaacagag tcaccaccgg aagcccccgg
     2161 aaagaacaga gggacggaaa gtcgccatcc acggctactg ccgccgcagc gccgtccatg
     2221 acgggaaaga gagcctccgg cgcacccacc gccagctcca cattcaaatg ttccgccagg
     2281 gaggcggcac gctcccggtc ttcttccaga gccgccagca ccgtccgtcc cggcagaagg
     2341 gaaaccagct ccctccactg ctccctgctc cattcactgg cgctccccag gcgggaaaac
     2401 ggagcgatta aaatgggggc gtcttccgcc ggggccgctt ttttcacagg gaacaaagac
     2461 ggggtggcga cgtccagccc atgcagcacc cccaattgca gataaagctg cacccggtgc
     2521 gccggggggg cggcgcgcag gacgggggcc tttacccgga acttgtactt ccgggcaccg
     2581 ggatgcgtgt cccgcccgga a
//
