GCA_009881475.1 - 12 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaDPPBNJOG_57 (original name was: gnl|Prokka|DPPBNJOG_57)

Region	Type	From	To	Most similar known cluster		Similarity
Region 57.1	lassopeptide	1	1,750

gnlProkkaDPPBNJOG_75 (original name was: gnl|Prokka|DPPBNJOG_75)

Region	Type	From	To	Most similar known cluster		Similarity
Region 75.1	cyclic-lactone-autoinducer	1	1,190

gnlProkkaDPPBNJOG_85 (original name was: gnl|Prokka|DPPBNJOG_85)

Region	Type	From	To	Most similar known cluster		Similarity
Region 85.1	lanthipeptide-class-ii	1	14,322

gnlProkkaDPPBNJOG_96 (original name was: gnl|Prokka|DPPBNJOG_96)

Region	Type	From	To	Most similar known cluster		Similarity
Region 96.1	NRPS	1	47,840	dipeptide aldehydes	NRP	88%

gnlProkkaDPPBNJOG_105 (original name was: gnl|Prokka|DPPBNJOG_105)

Region	Type	From	To	Most similar known cluster		Similarity
Region 105.1	ranthipeptide	41,335	62,923

gnlProkkaDPPBNJOG_112 (original name was: gnl|Prokka|DPPBNJOG_112)

Region	Type	From	To	Most similar known cluster		Similarity
Region 112.1	cyclic-lactone-autoinducer	22,803	40,352

gnlProkkaDPPBNJOG_126 (original name was: gnl|Prokka|DPPBNJOG_126)

Region	Type	From	To	Most similar known cluster		Similarity
Region 126.1	lanthipeptide-class-ii	1	15,007	xantholipin	Polyketide	4%
Region 126.2	cyclic-lactone-autoinducer	17,120	28,139

gnlProkkaDPPBNJOG_149 (original name was: gnl|Prokka|DPPBNJOG_149)

Region	Type	From	To	Most similar known cluster		Similarity
Region 149.1	cyclic-lactone-autoinducer	1	14,331

gnlProkkaDPPBNJOG_151 (original name was: gnl|Prokka|DPPBNJOG_151)

Region	Type	From	To	Most similar known cluster		Similarity
Region 151.1	RRE-containing	2,424	13,784

gnlProkkaDPPBNJOG_155 (original name was: gnl|Prokka|DPPBNJOG_155)

Region	Type	From	To	Most similar known cluster		Similarity
Region 155.1	cyclic-lactone-autoinducer	1,624	12,784

gnlProkkaDPPBNJOG_157 (original name was: gnl|Prokka|DPPBNJOG_157)

Region	Type	From	To	Most similar known cluster		Similarity
Region 157.1	cyclic-lactone-autoinducer	1	11,296

Region	Type	From	To	Most similar known cluster	Similarity
Region 57.1	lassopeptide	1	1,750
Region 75.1	cyclic-lactone-autoinducer	1	1,190
Region 85.1	lanthipeptide-class-ii	1	14,322
Region 96.1	NRPS	1	47,840	dipeptide aldehydes	NRP	88%
Region 105.1	ranthipeptide	41,335	62,923
Region 112.1	cyclic-lactone-autoinducer	22,803	40,352
Region 126.1	lanthipeptide-class-ii	1	15,007	xantholipin	Polyketide	4%
Region 126.2	cyclic-lactone-autoinducer	17,120	28,139
Region 149.1	cyclic-lactone-autoinducer	1	14,331
Region 151.1	RRE-containing	2,424	13,784
Region 155.1	cyclic-lactone-autoinducer	1,624	12,784
Region 157.1	cyclic-lactone-autoinducer	1	11,296

Compact view

gnlProkkaDPPBNJOG_57 - Region 1 - lassopeptide

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Location: 1 - 1,750 nt. (total: 1,750 nt) Show pHMM detection rules used

Region on contig edge.

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

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Lasso peptides

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Lasso peptides

Core peptide predictions

No core peptides found.

gnlProkkaDPPBNJOG_75 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 1,190 nt. (total: 1,190 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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No matches found.

gnlProkkaDPPBNJOG_85 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 14,322 nt. (total: 14,322 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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Lanthipeptides

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		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

gnlProkkaDPPBNJOG_96 - Region 1 - NRPS

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Location: 1 - 47,840 nt. (total: 47,840 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 47840:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg96_18

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaDPPBNJOG_105 - Region 1 - ranthipeptide

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Location: 41,335 - 62,923 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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No matches found.

gnlProkkaDPPBNJOG_112 - Region 1 - cyclic-lactone-autoinducer

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Location: 22,803 - 40,352 nt. (total: 17,550 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaDPPBNJOG_126 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 15,007 nt. (total: 15,007 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

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resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

Lanthipeptide predictions

Lanthipeptide(s) for ctg126_2

ctg126_5

Leader:

MKTCSERNEQRINSLQLSESLEDEIKELDGKNVEAGDVIFT

Core:

TDTSSFLTIVCC

Core with dehydrations:

DhbDDhbDhaDhaFLDhbIVCysCys

antiSMASH-DB 4.0 matches for ctg126_5

100.0% NZ_CP102267.1and 2 others:

NZ_JADMOE010000006.1 Lanthipeptides: I2G55_RS05425_lanthipeptideNZ_LT635469.1 Lanthipeptides: BQ7352_RS14540_lanthipeptide

Lanthipeptides: NQ550_RS10225_lanthipeptide

1

TDTSSFLTIVCC

12

TDTSSFLTIVCC

1

TDTSSFLTIVCC

12

50.0% NZ_CP113781.1: Lanthipeptides: GGADHKLB_RS14125_lanthipeptide

2

DTSSFLTIVCC

12

+ + FLTI+CC

2

NCTHFLTILCC

12

40.0% NZ_AP025566.1: Lanthipeptides: MWL92_RS00840_lanthipeptide

4

SSFLTIVCC

12

+SF TI+CC

7

TSFCTIICC

15

with 10 more sequence matches

33.3% NZ_CP036524.1: Lanthipeptides: LAJLEIBI_RS18025_lanthipeptide

4

SSFLTIVCC

12

++F TI+CC

7

TTFFTIICC

15

31.6% NZ_CP036170.1: Lanthipeptides: HDCHBGLK_RS18795_lanthipeptide

4

SSFLTIVCC

12

+FLTI CC

11

GTFLTIYCC

19

29.2% NZ_CP048626.1: Lanthipeptides: GXM18_RS04600_lanthipeptide

2

DTSSFLTIVCC

12

D +SF TI CC

14

DNASFFTIFCC

24

26.9% NZ_CP070036.1and 3 others:

NZ_JADMPO010000043.1 Lanthipeptides: I2I27_RS16805_lanthipeptideNZ_JADNGF010000061.1 Lanthipeptides: I2E06_RS15650_lanthipeptideNZ_JAQMLK010000037.1 Lanthipeptides: PNW18_RS16660_lanthipeptide

Lanthipeptides: I6K72_RS03970_lanthipeptide

4

SSFLTIVCC

12

S+FLTI CC

18

SAFLTIYCC

26

20.6% NZ_JADPCB010000035.1: Lanthipeptides: I4W51_RS14990_lanthipeptide

3

TSSFLTIVCC

12

T +FLTI+CC

25

TGAFLTILCC

34

17.9% NZ_CP094681.1: Lanthipeptides: K5I26_RS05295_lanthipeptide

1

TDTSSFLTIVCC

12

+D + FLTI CC

28

SDCALFLTIFCC

39

17.9% NZ_JAOQJV010000004.1: Lanthipeptides: OCV65_RS05205_lanthipeptide

4

SSFLTIVCC

12

S FLTI+CC

31

SGFLTIICC

39

17.6% NZ_MCGI01000004.1and 1 other:

NZ_MPDJ01000004.1 Lanthipeptides: BLA28_RS33805_lanthipeptide

Lanthipeptides: BEH84_RS33640_lanthipeptide

1

TDTSSFLTIVCC

12

T+++ FL+I CC

23

TESTPFLSIFCC

34

15.8% NZ_LT969517.1and 1 other:

NZ_QVLV01000012.1 Lanthipeptides: DXC51_RS29565_lanthipeptide

Lanthipeptides: C1733_RS32195_lanthipeptide

1

TDTSSFLTIVCC

12

T ++ FL+I CC

27

TASTGFLSIFCC

38

15.4% NZ_JADNBM010000025.1and 2 others:

NZ_JAQDZW010000013.1 Lanthipeptides: PGT55_RS13345_lanthipeptideNZ_QVMC01000003.1 Lanthipeptides: DWV46_RS15805_lanthipeptide

Lanthipeptides: I2A62_RS14145_lanthipeptide

4

SSFLTIVCC

12

++FLTI CC

31

TAFLTIFCC

39

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg126_5 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 1198.6 Da

Molecular weight: 1199.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1217.4 Da N = 1; 1235.4 Da N = 2; 1253.4 Da N = 3

gnlProkkaDPPBNJOG_126 - Region 2 - cyclic-lactone-autoinducer

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Location: 17,120 - 28,139 nt. (total: 11,020 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

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resistance

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaDPPBNJOG_149 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 14,331 nt. (total: 14,331 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaDPPBNJOG_151 - Region 1 - RRE-containing

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Location: 2,424 - 13,784 nt. (total: 11,361 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

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resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaDPPBNJOG_155 - Region 1 - cyclic-lactone-autoinducer

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Location: 1,624 - 12,784 nt. (total: 11,161 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

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resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaDPPBNJOG_157 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 11,296 nt. (total: 11,296 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.