GCA_013299975.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaKHMJAIAP_57 (original name was: gnl|Prokka|KHMJAIAP_57)

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	lanthipeptide-class-ii	75,533	98,226

gnlProkkaKHMJAIAP_68 (original name was: gnl|Prokka|KHMJAIAP_68)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	NRPS	1	36,043	dipeptide aldehydes	NRP	47%

gnlProkkaKHMJAIAP_77 (original name was: gnl|Prokka|KHMJAIAP_77)

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	RRE-containing	183,339	204,757

gnlProkkaKHMJAIAP_78 (original name was: gnl|Prokka|KHMJAIAP_78)

Region	Type	From	To	Most similar known cluster		Similarity
Region 36.1	ranthipeptide	1	15,672

gnlProkkaKHMJAIAP_79 (original name was: gnl|Prokka|KHMJAIAP_79)

Region	Type	From	To	Most similar known cluster		Similarity
Region 37.1	cyclic-lactone-autoinducer	32,801	48,556

Region	Type	From	To	Most similar known cluster	Similarity
Region 24.1	lanthipeptide-class-ii	75,533	98,226
Region 29.1	NRPS	1	36,043	dipeptide aldehydes	NRP	47%
Region 35.1	RRE-containing	183,339	204,757
Region 36.1	ranthipeptide	1	15,672
Region 37.1	cyclic-lactone-autoinducer	32,801	48,556

Compact view

gnlProkkaKHMJAIAP_57 - Region 1 - lanthipeptide-class-ii

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Location: 75,533 - 98,226 nt. (total: 22,694 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

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Lanthipeptide predictions

Lanthipeptide(s) for ctg24_89

ctg24_90

Leader:

MNEMNDKIIGNVEKELDEIEIIDTESIEEKLSFIVYG

Core:

TIVCC

Core with dehydrations:

DhbIVCysCys

allorf_077915_078043

Leader:

MLQLLTILLTPIYFRMHLQAG

Core:

QILTCLVVLMVIFIVTHYTCN

Core with dehydrations:

QILDhbCysLVVLMVIFIVDhbHYDhbCysN

allorf_095691_095804

Leader:

MTCCLMVMNIRKALSAG

Core:

KIHPTERASVFCPYFLVSGL

Core with dehydrations:

KIHPDhbERADhaVFCysPYFLVDhaGL

antiSMASH-DB 4.0 matches for allorf_095691_095804

100.0% NZ_JADMYT010000009.1and 3 others:

NZ_JADNMI010000013.1 Lanthipeptides: allorf_010454_010567_lanthipeptideNZ_QUEF01000006.1 Lanthipeptides: allorf_116827_116940_lanthipeptideNZ_QUIT01000007.1 Lanthipeptides: allorf_004751_004864_lanthipeptide

Lanthipeptides: allorf_115889_116002_lanthipeptide

1

KIHPTERASVFCPYFLVSGL

20

KIHPTERASVFCPYFLVSGL

1

KIHPTERASVFCPYFLVSGL

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg24_90 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 519.2 Da

Molecular weight: 519.7 Da

Number of bridges: 1

allorf_077915_078043 - Class II

Cleavage pHMM score: -4.90

RODEO score: 0

Monoisotopic mass: 2368.3 Da

Molecular weight: 2369.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2388.0 Da N = 1

allorf_095691_095804 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 2209.2 Da

Molecular weight: 2210.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2228.6 Da N = 1; 2246.6 Da N = 2

gnlProkkaKHMJAIAP_68 - Region 1 - NRPS

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Location: 1 - 36,043 nt. (total: 36,043 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 36043:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg29_8

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaKHMJAIAP_77 - Region 1 - RRE-containing

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Location: 183,339 - 204,757 nt. (total: 21,419 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

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gnlProkkaKHMJAIAP_78 - Region 1 - ranthipeptide

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Location: 1 - 15,672 nt. (total: 15,672 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaKHMJAIAP_79 - Region 1 - cyclic-lactone-autoinducer

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Location: 32,801 - 48,556 nt. (total: 15,756 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

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resistance

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Gene details

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Gene overview

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

No matches found.