GCA_013301655.1 - 9 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaNEMGPHMC_39 (original name was: gnl|Prokka|NEMGPHMC_39)

Region	Type	From	To	Most similar known cluster		Similarity
Region 39.1	cyclic-lactone-autoinducer	1	7,768

gnlProkkaNEMGPHMC_41 (original name was: gnl|Prokka|NEMGPHMC_41)

Region	Type	From	To	Most similar known cluster		Similarity
Region 41.1	cyclic-lactone-autoinducer	1	7,590

gnlProkkaNEMGPHMC_42 (original name was: gnl|Prokka|NEMGPHMC_42)

Region	Type	From	To	Most similar known cluster		Similarity
Region 42.1	cyclic-lactone-autoinducer	1	7,484

gnlProkkaNEMGPHMC_55 (original name was: gnl|Prokka|NEMGPHMC_55)

Region	Type	From	To	Most similar known cluster		Similarity
Region 55.1	ranthipeptide	35,708	57,296

gnlProkkaNEMGPHMC_76 (original name was: gnl|Prokka|NEMGPHMC_76)

Region	Type	From	To	Most similar known cluster		Similarity
Region 76.1	lanthipeptide-class-ii	1	22,307

gnlProkkaNEMGPHMC_84 (original name was: gnl|Prokka|NEMGPHMC_84)

Region	Type	From	To	Most similar known cluster		Similarity
Region 84.1	cyclic-lactone-autoinducer	1	3,944

gnlProkkaNEMGPHMC_109 (original name was: gnl|Prokka|NEMGPHMC_109)

Region	Type	From	To	Most similar known cluster		Similarity
Region 109.1	NRPS	103,026	152,010	dipeptide aldehydes	NRP	64%

gnlProkkaNEMGPHMC_181 (original name was: gnl|Prokka|NEMGPHMC_181)

Region	Type	From	To	Most similar known cluster		Similarity
Region 171.1	NRPS	17,031	48,030	equibactin	NRP	14%

gnlProkkaNEMGPHMC_303 (original name was: gnl|Prokka|NEMGPHMC_303)

Region	Type	From	To	Most similar known cluster		Similarity
Region 233.1	cyclic-lactone-autoinducer	1,974	18,130

Region	Type	From	To	Most similar known cluster	Similarity
Region 39.1	cyclic-lactone-autoinducer	1	7,768
Region 41.1	cyclic-lactone-autoinducer	1	7,590
Region 42.1	cyclic-lactone-autoinducer	1	7,484
Region 55.1	ranthipeptide	35,708	57,296
Region 76.1	lanthipeptide-class-ii	1	22,307
Region 84.1	cyclic-lactone-autoinducer	1	3,944
Region 109.1	NRPS	103,026	152,010	dipeptide aldehydes	NRP	64%
Region 171.1	NRPS	17,031	48,030	equibactin	NRP	14%
Region 233.1	cyclic-lactone-autoinducer	1,974	18,130

Compact view

gnlProkkaNEMGPHMC_39 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 7,768 nt. (total: 7,768 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaNEMGPHMC_41 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 7,590 nt. (total: 7,590 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaNEMGPHMC_42 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 7,484 nt. (total: 7,484 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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resistance

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gnlProkkaNEMGPHMC_55 - Region 1 - ranthipeptide

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Location: 35,708 - 57,296 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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gnlProkkaNEMGPHMC_76 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 22,307 nt. (total: 22,307 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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Lanthipeptides

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Lanthipeptide predictions

Lanthipeptide(s) for ctg76_10

ctg76_9

Leader:

MNEMNDKIIGNVEKELDEIEIIDTESIEEKLSFIVYG

Core:

TIVCC

Core with dehydrations:

DhbIVCysCys

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg76_9 - Class II: Cleavage pHMM score: -5.90; RODEO score: 0; Monoisotopic mass: 519.2 Da; Molecular weight: 519.7 Da; Number of bridges: 1

gnlProkkaNEMGPHMC_84 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 3,944 nt. (total: 3,944 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

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gnlProkkaNEMGPHMC_109 - Region 1 - NRPS

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Location: 103,026 - 152,010 nt. (total: 48,985 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 103025 - 152010:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg109_136

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaNEMGPHMC_181 - Region 1 - NRPS

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Location: 17,031 - 48,030 nt. (total: 31,000 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 17030 - 48030:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys) + (Me-Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg171_21

AMP-binding (27..424): X

nrpys: diOH-Bz, Sal

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TLPSIGQIMK
Nearest Stachelhaus code(s):: n-oh-nitropheBut TLPSQGVVCK (53% 8Å match); diOH-Bz or Sal TLPAQGVIAK (50% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg171_33

Search NORINE for peptide: strict or relaxed

AMP-binding (526..926): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLANISQIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (74% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg171_34

Search NORINE for peptide: strict or relaxed

AMP-binding (557..957): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNMSMIWK
Nearest Stachelhaus code(s):: Cys DLYNMSMIWK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaNEMGPHMC_303 - Region 1 - cyclic-lactone-autoinducer

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Location: 1,974 - 18,130 nt. (total: 16,157 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

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resistance

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