GCA_013304785.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaDLDDNNCP_19 (original name was: gnl|Prokka|DLDDNNCP_19)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	ranthipeptide	55,422	77,010

gnlProkkaDLDDNNCP_60 (original name was: gnl|Prokka|DLDDNNCP_60)

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	lanthipeptide-class-ii	70,910	93,591

gnlProkkaDLDDNNCP_79 (original name was: gnl|Prokka|DLDDNNCP_79)

Region	Type	From	To	Most similar known cluster		Similarity
Region 33.1	NRPS	20,395	55,811	dipeptide aldehydes	NRP	47%

gnlProkkaDLDDNNCP_83 (original name was: gnl|Prokka|DLDDNNCP_83)

Region	Type	From	To	Most similar known cluster		Similarity
Region 36.1	cyclic-lactone-autoinducer	162,813	183,562

Region	Type	From	To	Most similar known cluster	Similarity
Region 16.1	ranthipeptide	55,422	77,010
Region 25.1	lanthipeptide-class-ii	70,910	93,591
Region 33.1	NRPS	20,395	55,811	dipeptide aldehydes	NRP	47%
Region 36.1	cyclic-lactone-autoinducer	162,813	183,562

Compact view

gnlProkkaDLDDNNCP_19 - Region 1 - ranthipeptide

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Location: 55,422 - 77,010 nt. (total: 21,589 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaDLDDNNCP_60 - Region 1 - lanthipeptide-class-ii

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Location: 70,910 - 93,591 nt. (total: 22,682 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg25_86

ctg25_87

Leader:

MTEEFIGCIEEELKEIELFYEENGVEVEAYTLHNG

Core:

SFFSICC

Core with dehydrations:

DhaFFDhaICysCys

antiSMASH-DB 4.0 matches for ctg25_87

100.0% NZ_JADPAT010000014.1and 2 others:

NZ_JAQDKC010000001.1 Lanthipeptides: PGQ53_RS00010_lanthipeptideNZ_QUIY01000001.1 Lanthipeptides: allorf_008439_008567_lanthipeptide

Lanthipeptides: allorf_07424_07552_lanthipeptide

1

SFFSICC

7

SFFSICC

1

SFFSICC

7

ctg25_95

Leader:

MKSNYKIFTAVFLSAIMTA

Core:

AAPLTVCGAEVFSAGESFSDGTGSIPAILTDMVLPDGEIGTEYQVQL

Core with dehydrations:

AAPLDhbVCysGAEVFDhaAGEDhaFDhaDGDhbGDhaIPAILDhbDMVLPDGEIGDhbEYQVQL

antiSMASH-DB 4.0 matches for ctg25_95

100.0% NZ_CZBP01000025.1: Lanthipeptides: ARA22_RS13915_lanthipeptide

1

AAPLTVCGAEVFSAGESFSDGTGSIPAILTDMVLPDGEIGTEYQVQL

47

AAPLTVCGAEVFSAGESFSDGTGSIPAILTDMVLPDGEIGTEYQVQL

1

AAPLTVCGAEVFSAGESFSDGTGSIPAILTDMVLPDGEIGTEYQVQL

47

17.0% NZ_CP035042.1: Lassopeptides: allorf_3065580_3065849_lassopeptide

4

LTVCGAEVFSAGESFSDGTGSIPA

27

LT G++VF S+G+ + P

3

LTRPGSDVFPGNAPHSNGSRNPPG

26

14.9% NZ_VTZW01000015.1: Lanthipeptides: allorf_204916_205050_lanthipeptide

9

AEVFSAGESFSD

20

AE++ GE+FSD

5

AELWERGEAFSD

16

ctg25_98

Leader:

MGDKRLYKSSENSMLCGVCGG

Core:

IAEYFDIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

Core with dehydrations:

IAEYFDIDPDhbLVRLAWVILDhbCysFGGAGIWAYIIAAIIIPKR

MIBiG 3.1 matches for ctg25_98

12.5% BGC0000517 (BAB04173.1): Lanthipeptide: haloduracin β or haloduracin α

14

LAWVILTC-FGGAGIWAYIIAAII

36

AW ++C G G + + +

1

CAWYNISCRLGNKGAYCTLTVECM

24

antiSMASH-DB 4.0 matches for ctg25_98

100.0% NZ_CZBP01000025.1: Lanthipeptides: ARA22_RS13900_lanthipeptide

1

IAEYFDIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

40

IAEYFDIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

1

IAEYFDIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

40

15.9% NZ_LMEQ01000012.1: Lanthipeptides: allorf_0980337_0980543_lanthipeptide

2

AEYFDIDP

9

AE FDIDP

15

AEAFDIDP

22

14.3% NZ_MOWR01000001.1: Lanthipeptides: BMI76_RS01735_lanthipeptide

16

WVILTCFGGAGIWA

29

+V L C GAG++A

28

YVTLACAAGAGVYA

41

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg25_87 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 769.3 Da

Molecular weight: 769.9 Da

Number of bridges: 2

ctg25_95 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 4611.3 Da

Molecular weight: 4614.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4632.1 Da N = 1; 4650.1 Da N = 2; 4668.1 Da N = 3; 4686.2 Da N = 4; 4704.2 Da N = 5; 4722.2 Da N = 6; 4740.2 Da N = 7

ctg25_98 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 4425.5 Da

Molecular weight: 4428.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4446.3 Da N = 1

gnlProkkaDLDDNNCP_79 - Region 1 - NRPS

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Location: 20,395 - 55,811 nt. (total: 35,417 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 20394 - 55811:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg33_44

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaDLDDNNCP_83 - Region 1 - cyclic-lactone-autoinducer

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Location: 162,813 - 183,562 nt. (total: 20,750 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.