LOCUS       gnlProkkaABFKIMLF_143   5169 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ABFKIMLF_143.
ACCESSION   gnlProkkaABFKIMLF_143
VERSION     gnlProkkaABFKIMLF_143
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 05:37:13
            Original ID  :: gnl|Prokka|ABFKIMLF_143
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5169
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5169
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1681:4886]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1682..4886
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5169
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5169
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(3..689)
                     /locus_tag="ctg143_1"
                     /transl_table=11
                     /translation="MKYLIITVAGTATRFNRDTKEETLKCLYYTDEPQYALLAQLLKNC
                     GEYDKYILVGGYLYEKLGRFVKNELSGYGKKIELVYNEHFKDYGSGYSLYKGIEAIKEA
                     GDVTFVEGDLFFMATDFRQVYNSPKSVITINREPIYSNKAVALYINVDGKPRYLYDTNH
                     QTLTVSEPFKAIFNSGQMWKFSSSERLLKTVDSLTKEQLQGTNLEIIQAYFSDMNSEEY
                     DVVTFTD"
     CDS             complement(686..1681)
                     /NRPS_PKS="Domain: NAD_binding_4 (83-198). E-value:
                     6.4e-10. Score: 30.4. Matches aSDomain:
                     nrpspksdomains_ctg143_2_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     143.1; E-value: 2.2e-43)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg143_2"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 4.2e-10,
                     bitscore: 27.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.5e-09, bitscore:
                     25.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLYVISGVTGMTGSELSRQFVSQGHTVIGFDNFFASSIDTVKD
                     IIDNPSFIFYNYDINKVAQMDEVCDKAKELKIECDGRLVFINCAAVVHTKHFYEVESTF
                     QTNVLAMKDFLERAISMRADTYINCSTSEVYSMQSWNENGGVKESDYLAMSTAEHSQRT
                     SYACGKLMTEFFMKDAVGKGRIKGCSIRFANVYSRHERFAEHIIPHIIDNLLESHTVTL
                     LENSKVNKRTFLHNIDSCRAVMELIASEDALDGTVYNVATEEEIAIIDLVKTIAIKMGI
                     NDVDIRFEGYRKSDPERRLLNTDKIRTRTSWKPIITLDEGLTMCVNSIKK"
     aSDomain        complement(1088..1432)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg143_2_NAD_binding_4.1"
                     /evalue="6.40E-10"
                     /label="ctg143_2_NAD_binding_4.1"
                     /locus_tag="ctg143_2"
                     /protein_end="198"
                     /protein_start="83"
                     /score="30.4"
                     /tool="antismash"
                     /translation="VFINCAAVVHTKHFYEVESTFQTNVLAMKDFLERAISMRADTYIN
                     CSTSEVYSMQSWNENGGVKESDYLAMSTAEHSQRTSYACGKLMTEFFMKDAVGKGRIKG
                     CSIRFANVYSR"
     CDS             complement(1682..2410)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg143_3"
                     /sec_met_domain="Metallophos_2 (E-value: 1.9e-28, bitscore:
                     88.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVILFADIHANLSAFEAVLADIEKHYQPDAMISLGDFIDYGMRS
                     NEIIERIQTVNIPLLANLKGNHEKALIDGELSCFSSERARVMSKYTRNHLTQTSIDYIS
                     NVMQEPMQELEIGGKKVLLLHGTIDDPYWGKLTPAKVEDEKYQDFDYVLSGHIHQPFKI
                     DVFFKVDNPIMRNQKRTTFINPGSVGQPRNQNPYAQYVYINFKTGETHFNAVPYDIEAE
                     QSLFPKEIDTFYSERLKFGI"
     CDS             complement(2414..3541)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 74.7;
                     E-value: 1.1e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg143_4"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.1e-21, bitscore:
                     63.8, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.6e-24, bitscore:
                     74.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRPEFFVNILKEQGIDFYAGVPDSLLKNICAYITDHLPAEQNII
                     AANEGGAMGIAVGYHLATGKVPVVYMQNSGEGNIINPFASLTDKDVYNIPVLLVIGWRG
                     RPGVHDEPQHVKQGKVTIGLLNTMGINYAILPKDEEGAAKQIKIAVDFMKATNECYALV
                     IEKDTFDTYKLQSVEVNELAMSREETIQKVASNIEDNACIVSTTGMISRELFEARTAWS
                     QGHERDFLTVGAMGHASQIALGIALQKPERRVYCFDGDGASIMHMGNMAITASMKCKNY
                     VHIVFNNGAHDSVGGQPTVGLKIDFCAIAQAVGYKAAYSVETMEALEAKLAEFKNAEGP
                     VFLQICVKKGNRKDLGRPTTTPIQNKEAFMEFLNK"
     CDS             complement(3576..4886)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 190.9;
                     E-value: 4.6e-58)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg143_5"
                     /sec_met_domain="CTP_transf_like (E-value: 5.1e-13,
                     bitscore: 37.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 5.9e-115, bitscore:
                     371.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 3.7e-62, bitscore:
                     198.3, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTVYLGMIGDIMHPGYINIINKATEYGDVIIGLFTDKAIANHR
                     RLPYLTWEQRKNVVESIRNVSRVVPQDDWSYVSNLLKYKPDYIIHGDDWQVGPDKYIRD
                     EVFKVMEKLGGEVIEIPYTQNISASGIKQEIDALGVTPQMRLSSLRRLIAAKPIVRILE
                     SHNGLTGLIAEHTKVEVNGQEREFDGMWASSLTDSTSKGKPDIEAVDLTTRLHDLNDTL
                     EVTTKPVIFDGDTGGKIEHFGFTVRTLERLGISCVIIEDKVGLKQNSLFGTDAVQTQDT
                     IEGFQAKIRAGKEAQITRDFMIVSRCESLIAGKTVDDALERCHAYVEAGTDGIMIHSKE
                     KSGEDIKEFCLRFREKNAHTPIIVVPTTYNQFTEEELAEWGINVVIYANHMLRSAYPAM
                     VKCAETILYTSRSLEASEQYCMPIKQILNLIPGTKKK"
ORIGIN
        1 cagtcagtga aagtcactac atcatactct tcactattca tatcactaaa gtaagcctga
       61 attatttcaa gattagtacc ttgaagttgt tcttttgtaa gcgaatcaac agtcttcaaa
      121 agcctctcag atgaagaaaa tttccacatc tgaccactgt tgaagattgc cttgaatggc
      181 tctgatacag tcaatgtctg atgattcgta tcatataaat accttggttt gccatcaacg
      241 ttaatgtata acgcaactgc cttattcgag tagattggtt ctcggttaat ggtaatcaca
      301 ctctttggcg aattatacac ttgcctgaaa tctgtagcca taaagaacaa gtcgccttct
      361 acgaaagtca catcacctgc ctcctttatt gcctctatac ctttgtataa actgtagccg
      421 gagccatagt ctttgaaatg ctcgttgtaa acaagttcta tttttttgcc ataaccagag
      481 agttcgttct ttacaaatct tcccaacttc tcataaaggt agccccccac aaggatatat
      541 ttatcgtact caccacaatt cttcagcaat tgagcaagaa gagcgtattg aggttcatcg
      601 gtataataca agcatttgag ggtttcttcc ttagtgtcgc gattaaaacg cgtagccgtt
      661 ccagcaacag ttataatcaa atatttcatt tcttgatact atttacacac atcgtgaggc
      721 cttcatcgag agtgatgata ggtttccagc tagtacgggt acgaatcttg tccgtattga
      781 gcaatcgacg ctcaggatca gactttcggt aaccttcgaa acggatatcc acatcgttga
      841 tgcccatttt tattgcaatc gtcttgacta gatcaataat ggcaatttcc tcttcagtag
      901 ccacattata aacagtacca tcaagagcat cctcagaagc gatgagctcc ataacggcac
      961 gacagctatc aatattatga aggaaggtac gtttgttcac cttcgagttt tcaagaaggg
     1021 ttacggtatg gctttcaagc agattatcaa tgatatgagg gataatgtgc tctgcaaaac
     1081 gctcgtgcct gctatataca ttagcaaagc gaatagaaca acctttaatt cgtcccttgc
     1141 caacagcatc tttcataaag aattcagtca tcaacttgcc gcaagcataa cttgtacgtt
     1201 ggctatgttc cgctgttgac attgccaaat aatcagattc tttcacaccg ccattctcat
     1261 tccaagactg cattgagtaa acctccgaag ttgaacaatt aatgtaagta tcagccctca
     1321 tagaaatggc acgctccaag aaatccttca tagcaaggac gttagtctga aaagtactct
     1381 ctacctcata gaaatgcttt gtatggacaa cagcagcaca attgataaac accagacgac
     1441 catcgcactc aattttgagt tctttcgcct tatcgcatac ttcatccatc tgagcgactt
     1501 tgttaatgtc gtaattatag aagatgaacg agggattgtc aataatgtct ttaacggtat
     1561 caatagaact tgcaaagaag ttgtcgaaac cgattaccgt atgaccctga gaaacaaact
     1621 gacgagacag ttcactaccc gtcatgccag taacaccact tattacatat aagttcttca
     1681 tttatattcc aaattttaat cgttcgctgt aaaaagtatc tatctctttg gggaaaagag
     1741 attgttcagc ttcaatatcg taaggaactg cattgaaatg ggtttccccc gttttaaaat
     1801 tgatatacac atattgtgca tatggattct gattgcgagg ttgtccaact gaacctgggt
     1861 tgataaaagt cgtacgtttc tgattgcgca tgattggatt gtcaactttg aagaatacat
     1921 ctatcttaaa aggttgatga atgtgaccgc tcaatacgta gtcaaaatcc tgatactttt
     1981 catcttccac ttttgctgga gtcagttttc cccaataggg atcatcaata gttccatgta
     2041 aaagaagtac tttcttacca ccaatttcca actcttgcat tggctcttgc atcacattgc
     2101 ttatataatc aatagatgtc tgagtgagat ggtttcttgt gtatttggac atcacacgag
     2161 ctctttctga agagaaacat gagagttccc catcaatcaa agccttctcg tgattgccct
     2221 tcaaattggc aagtaatgga atattaacag tttgtatacg ttcaataatt tcatttgaac
     2281 gcataccata gtcgataaag tcacctagac taatcatggc atctggctga tagtgtttct
     2341 caatatcagc tagaaccgcc tcaaaagcag aaagattggc gtgtatatca gcaaacagaa
     2401 ttactttcat actttacttg ttaagaaatt ccataaaagc ctctttgttc tgaataggag
     2461 ttgtagttgg acggccaagg tccttgcggt ttcccttttt tacgcaaatc tgaaggaaaa
     2521 caggaccttc tgcgttcttg aattctgcaa gttttgcttc aagtgcctcc atagtttcta
     2581 cactataagc agccttgtag cctacagctt gagcaattgc acaaaaatca atcttgagac
     2641 caacagtagg ctgaccacca acagaatcgt gtgcgccatt gttgaacaca atgtgcacat
     2701 aattcttgca tttcatgctg gctgtaatag ccatattacc catgtgcatg atgctggctc
     2761 catcaccatc aaaacaatat actcgacgct caggcttttg gagagctatg ccgagagcta
     2821 tctgggaagc atggcccatc gcccctacag taaggaaatc acgttcatga ccttggctcc
     2881 aagcagtacg tgcctcaaag agctcgcgag aaatcatacc agtagttgat acgatacaag
     2941 cattatcttc aatgtttgaa gccaccttct gaatcgtctc ttcgcgagac attgccagtt
     3001 cgtttacctc aacgctctga agcttatagg tatcgaaagt atccttctct atcacaaggg
     3061 catagcactc gttggttgcc ttcatgaagt cgacagctat ttttatctgc tttgcagctc
     3121 cctcttcatc ctttggcaga atagcatagt tgatacccat ggtattaagc aaaccgattg
     3181 tcaccttacc ttgcttcaca tgctgaggtt catcgtgaac tccagggcgc cctctccaac
     3241 cgataacgag gagcacagga atgttataaa cgtccttgtc ggtaagagag gcaaacggat
     3301 taatgatatt gccttcacca gagttctgca tataaactac aggcacctta cctgttgcca
     3361 aatgataacc tacagcaata cccatggccc ctccttcgtt agcagcaata atattctgct
     3421 cagcaggaag gtggtcggta atatatgcac aaatgttctt gagcaacgaa tcaggaacac
     3481 cagcataaaa gtctatacct tgttccttga gtatatttac aaaaaattcg ggtcttacca
     3541 tttgtaaaat agtttaaggg gtaagaaatt aatgtttact tcttctttgt gcctggaata
     3601 agattaagaa tctgtttgat aggcatacag tactgctcag atgcttcaag cgaacggcta
     3661 gtatagagga tggtttctgc acatttaacc attgcaggat aagcagaacg aagcatgtga
     3721 ttagcataaa tcactacatt aataccccat tcagcaagtt cctcttctgt gaactggtta
     3781 taagtagtag gcacaacgat gataggagta tgagcattct tctcgcgaaa gcgcaagcaa
     3841 aactccttga tgtcctctcc agacttctct ttggagtgga tcattatacc gtcagtacct
     3901 gcctctacgt aagcatgaca acgctccaaa gcatcatcta cagtcttacc agcaatgaga
     3961 gactcacaac ggctgacaat catgaagtca cgagtaatct gcgcctcctt acccgcacgg
     4021 attttagcct gaaaaccttc gattgtatcc tgagtctgta cagcatcagt accaaaaagt
     4081 gaattctgtt tcaagcctac cttatcttca attataacgc aagagattcc caagcgctca
     4141 agggtgcgaa cggtaaaacc gaagtgttca atcttgccac cagtatcgcc atcgaagata
     4201 acaggttttg tggttacttc aagggtatca ttgaggtcat ggagacgggt agtcaaatcg
     4261 acagcctcaa tatcaggctt acccttagag gtagagtcgg taagggagga agcccacatg
     4321 ccatcgaatt cacgctcctg accattcacc tctaccttag tatgttctgc tatgaggcca
     4381 gtaagaccat tgtgactttc aagaatacgt acaatcggtt tagctgcaat aaggcgacgg
     4441 agagaagaaa gacgcatctg aggagttaca ccaagagcat caatctcttg tttgatacca
     4501 gaagcagaga tgttttgggt gtaaggaatc tcgataacct caccacccaa cttttccatt
     4561 actttgaata cctcatcacg gatatacttg tcaggaccaa cctgccaatc atcaccatga
     4621 atgatgtaat caggcttgta cttaaggaga ttagagacat agctccaatc atcctgaggg
     4681 accacccttg atacgttcct gatgctctca acaacgttct ttctttgctc ccaagtgaga
     4741 taaggaagtc tacggtggtt agcaatcgcc ttatcagtga acaaaccgat tatcacatcg
     4801 ccatactcag tagctttgtt aatgatgtta atgtaacccg gatgcatgat atcgcctatc
     4861 ataccaagat aaactgtctt tgccattatt atttaacatt tttcaaatta ttataaagag
     4921 catctatagt caatcacctc aatttctcct gcttgagaat aagtatacta cacatagata
     4981 gatttaccat aagttcgact acacttctga accactcaat aagtttctcc atttgcggag
     5041 agcaaaggaa ctgtaggact ctaccattgt gctaaaagaa gaatggatat accattgagt
     5101 tggagtaaca atgatgactg tctcttatac acatctgacg ctgccgacga cgcccatcgt
     5161 ccgtgtaga
//
