LOCUS       c00362_gnlPro..         3559 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GDINKNGM_362.
ACCESSION   c00362_gnlPro..
VERSION     c00362_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 11:40:17
            Original ID  :: gnl|Prokka|GDINKNGM_362
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3559
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3559
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[111:3242]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      112..3242
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3559
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3559
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             112..2379
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg362_1"
                     /sec_met_domain="Gly_radical (E-value: 9.8e-42, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.6e-262, bitscore:
                     859.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEKTQMQNVDVKDAWEGFRGKEWKEEIDIREFIQDNFTPYDGDQ
                     SFLEGPTEATTKLNDKLIDLKLKERAHGGPLEADTKVVSTITSHKPGYLDKSLEKIVGL
                     QTDKPMKRALMPYGGIRMAKGALEAYGFKIDPETDFVFENLSKTHNQGVFDAYTSEIMK
                     ARHNKIITGLPDAYGRGRLIGDFPRVALYGIDRLIEEKKKDLENCGDGEMTNDIIQMRE
                     EISDQIKALNDMKIMAESYGYDISKPATTAKEAIQWLYFGYLASIKQQNGAAMSIGRID
                     SFIDIYIERDMKRGILTEKEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIA
                     GMGLDGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQ
                     YENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQ
                     VGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKDT
                     LVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRADD
                     IAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGAN
                     PAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYM
                     RKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHT
                     SM"
     CDS             2406..3242
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.4;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg362_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-34, bitscore:
                     107.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVVFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYHEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
     CDS             complement(3379..3558)
                     /locus_tag="ctg362_3"
                     /transl_table=11
                     /translation="HKTEIENSFVLPRLTNGPIEGVNNHIKVIKRIAYGYNNFKHFRLR
                     ILISLKNNVIFFST"
ORIGIN
        1 ttgaaaataa aaaacattgt gaacgatttt tcattgaatt taatttaact aaggtgtagt
       61 attaacagtg tagaaaacgt tttcttaaaa atcacaaagg aggctttgag catggttgaa
      121 aagactcaaa tgcaaaacgt tgatgtaaaa gatgcttggg aaggatttag aggaaaagaa
      181 tggaaagaag aaattgatat tcgtgaattt attcaagata acttcactcc atatgatgga
      241 gatcaatctt tccttgaagg accaaccgaa gctacaacga agttaaacga taaattaatc
      301 gacttaaaat taaaagaacg tgcacatggt ggacctcttg aagctgatac aaaagtagta
      361 tcaacaatta catctcataa accaggttat ttagacaagt cattagaaaa gattgttggt
      421 ttacaaacag ataaacctat gaaacgtgca cttatgccat atggtggtat cagaatggct
      481 aaaggtgctt tggaagctta tggattcaag attgatccag aaacagactt cgtatttgaa
      541 aatctatcta agacgcataa ccaaggtgta tttgatgcat acacaagcga aatcatgaaa
      601 gctcgccaca ataagattat cactggacta ccagatgcat atggtcgtgg ccgtttgatt
      661 ggtgacttcc cacgtgttgc attgtatggt attgatcgtt taatcgaaga aaagaaaaaa
      721 gatttggaaa attgtggcga tggtgagatg actaacgaca taatccaaat gcgtgaagag
      781 atttctgacc aaatcaaagc cttaaatgat atgaagatta tggctgagtc ttatggctat
      841 gacatttcca aaccagctac aacagctaaa gaagcaattc aatggttata ctttggatat
      901 ttagcatcta ttaaacaaca aaatggtgct gctatgtcta ttggtcgtat cgattcattc
      961 atcgatattt acattgaacg tgatatgaag cgtggtattt taactgaaaa agaagcacaa
     1021 gaattaattg accaattcac aatgaaattg agaatggtat gcttcattcg tacaccagct
     1081 tacaactcac tattctcagg taaccctatc tgggcaacat tatcaattgc tggtatgggc
     1141 ttagatggac gtcaccatgt aactaagaca tcatatcgtt tcttaaatac attgcataat
     1201 atgggtgccg caccagaacc aaatatcaca ttactttggt ctgatcgttt gcctgaatca
     1261 tttaagcact atgctgcaag tgtatctatt gcaagttcta caattcaata cgaaaacgat
     1321 gacttaatga tgaagacatg gggaacagac tactatggca ttgcttgctg tgtatctgca
     1381 caaccaattg caaatggtat gcaattcttc ggagctcgtg caaatctagc taaggctgta
     1441 ttgtatgcta tcaatggtgg taaggatgaa cttacaaaac aacaagtagg tcctgcatac
     1501 gaaccaatta gctcagaata cattgactac gatgaattca tgaagaaatt cgatgacatg
     1561 atggaatgga ttgctgatat ttatgtaaat gctttgaaca caattcatta catgcatgac
     1621 aagtattact atgaatcagc acaaatggca ttgaaagata cattagtaga tcgtagcttt
     1681 gctacaggta tttctggatt gtctgttgct gctgactcaa tttcagctat caagtacgga
     1741 catgttaaag taattcgtga cgaagatggt attgctgttg actttaaagc agatcatgac
     1801 ttccctcgtt atggtaacga tgatgatcgt gctgacgata ttgctgcatg gcttgttaag
     1861 gaactattta ctaagatgag caagcatcat ctatatcgtg gagctaagtt aacaacatcc
     1921 gttcttacaa ttacttcaaa tgttgtttat ggtaagaaca caggtactac tccaaatggt
     1981 cgtcaaaagg gtgaaccatt tgcaccaggt gctaacccag catacggagc agatacaaca
     2041 ggtgctttag catcattatt atctgttgct aaattaccat acaagtatgc tacagatggt
     2101 atttctaata catttgctgt aacaccaaca acattgggtc atgatgaaaa gatgcaagaa
     2161 gatacattgg taaacatgat cggaggctat atgagaaaag atggtcatca tttgaatatt
     2221 aatgtattca atagagatac tttattagat gctcaagcac atccagagaa gtatccaaca
     2281 ttaacaattc gtgtttcagg ttactgtgta tactttgctg atctaactaa agaacaacaa
     2341 gatgacgtta ttgcaagaac cttccatact tcaatgtaat atttagaaga gagtaggtgg
     2401 agagaatgag tgctaatcct ttattgaatc cagttccaac taaagatggt catgtcgtag
     2461 gctatgtaca ttctttggaa acttttgggg ctgtcgatgg accaggtata cgttttgtcg
     2521 tttttatgca aggttgccat atgaggtgta agttctgtca caatccggac acttggaaaa
     2581 caagggtagg ttcacaaatg acgactgaag aagttctaaa taaggcactt ccttatcgta
     2641 gcttttgggg cgacaaaggt ggaataactt tatcaggtgg tgaaattcta ttgcaaatag
     2701 attttgcact agagttattc aagatgtgca aggaagaagg tattagcact tgtttggaca
     2761 cttgtggaca gccatttaca agaagagaac cttggtttag taagttcaat gagttaatgg
     2821 actataccga tattctatta gttgatatca agcatattaa ttcggacgaa cataagagat
     2881 taaccggatt cccaaatgat aatattttgg atatgtgcga atatctttct agcataggga
     2941 aacctgtttg gattcgtcat gtccttatcc caggcatcac ggataatgat gaatacttga
     3001 aacaattggg cgagtatgtt aaggaaaatc ttcataatgt cgaaaaattt gaagtattac
     3061 cttatcatac aatgggcgtt cataagtatc atgaaatggg tatcagatat cgtcttgaag
     3121 gtgttgagcc tcctacgcca gaaagagtaa aaaatgctga aaaactgcta gaagtagata
     3181 gatatcaagg atataaagat tggtatccag gcattaatac tttggatcaa caaccaagct
     3241 agttaaattg gcaaatacaa atataaaata aaaacatggt atcgcttagt ttttacggtt
     3301 ctttgtcaaa aaggactgat gccaaaattt taattattat tttcaaacta ttagatgaaa
     3361 gtctaatagt tttttgtttt aagtagagaa gaaaattaca ttatttttta gcgaaatcaa
     3421 aatcctgagt ctaaaatgct taaaattatt atagccatag gctatacgct taattacttt
     3481 aatgtgatta ttaacgcctt caataggacc attggtaagt ctaggtaaaa caaaactatt
     3541 ttctatttct gtcttatgt
//
