LOCUS       c00135_gnlPro..         2358 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DHCELJLP_135.
ACCESSION   c00135_gnlPro..
VERSION     c00135_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 11:00:12
            Original ID  :: gnl|Prokka|DHCELJLP_135
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2358
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2358
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2236]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2236
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2358
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2358
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1373
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg135_1"
                     /sec_met_domain="Gly_radical (E-value: 2.9e-42, bitscore:
                     130.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.9e-141, bitscore:
                     458.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIAGMGL
                     DGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQYEND
                     DLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQVGPA
                     YEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKDTLVDR
                     SFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRADDIAAW
                     LVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGANPAYG
                     ADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYMRKDG
                     HHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHTSM"
     CDS             1400..2236
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.4;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg135_2"
                     /sec_met_domain="Radical_SAM (E-value: 8.4e-35, bitscore:
                     107.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVVFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYHEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
ORIGIN
        1 aaaaagaagc acaagaatta attgaccaat tcacaatgaa attgagaatg gtatgcttca
       61 ttcgtacacc agcttacaac tcactattct caggtaaccc tatctgggca acattatcaa
      121 ttgctggtat gggcttagat ggacgtcacc atgtaactaa gacatcatat cgtttcttaa
      181 atacattgca taatatgggt gccgcaccag aaccaaatat cacattactt tggtctgatc
      241 gtttgcctga atcatttaag cactatgctg caagtgtatc tattgcaagt tctacaattc
      301 aatacgaaaa cgatgactta atgatgaaga catggggaac agactactat ggcattgctt
      361 gctgtgtatc tgcacaacca attgcaaatg gtatgcaatt cttcggagct cgtgcaaatc
      421 tagctaaggc tgtattgtat gctatcaatg gtggtaagga tgaacttaca aaacaacaag
      481 taggtcctgc atacgaacca attagctcag aatacattga ctacgatgaa ttcatgaaga
      541 aattcgatga catgatggaa tggattgctg atatttatgt aaatgctttg aacacaattc
      601 attacatgca tgacaagtat tactatgaat cagcacaaat ggcattgaaa gatacattag
      661 tagatcgtag ctttgctaca ggtatttctg gattgtctgt tgctgctgac tcaatttcag
      721 ctatcaagta cggacatgtt aaagtaattc gtgacgaaga tggtattgct gttgacttta
      781 aagcagatca tgacttccct cgttatggta acgatgatga tcgtgctgac gatattgctg
      841 catggcttgt taaggaacta tttactaaga tgagcaagca tcatctatat cgtggagcta
      901 agttaacaac atccgttctt acaattactt caaatgttgt ttatggtaag aacacaggta
      961 ctactccaaa tggtcgtcaa aagggtgaac catttgcacc aggtgctaac ccagcatacg
     1021 gagcagatac aacaggtgct ttagcatcat tattatctgt tgctaaatta ccatacaagt
     1081 atgctacaga tggtatttct aatacatttg ctgtaacacc aacaacattg ggtcatgatg
     1141 aaaagatgca agaagataca ttggtaaaca tgatcggagg ctatatgaga aaagatggtc
     1201 atcatttgaa tattaatgta ttcaatagag atactttatt agatgctcaa gcacatccag
     1261 agaagtatcc aacattaaca attcgtgttt caggttactg tgtatacttt gctgatctaa
     1321 ctaaagaaca acaagatgac gttattgcaa gaaccttcca tacttcaatg taatatttag
     1381 aagagagtag gtggagagaa tgagtgctaa tcctttattg aatccagttc caactaaaga
     1441 tggtcatgtc gtaggctatg tacattcttt ggaaactttt ggggctgtcg atggaccagg
     1501 tatacgtttt gtcgttttta tgcaaggttg ccatatgagg tgtaagttct gtcacaatcc
     1561 ggacacttgg aaaacaaggg taggttcaca aatgacgact gaagaagttc taaataaggc
     1621 acttccttat cgtagctttt ggggcgacaa aggtggaata actttatcag gtggtgaaat
     1681 tctattgcaa atagattttg cactagagtt attcaagatg tgcaaggaag aaggtattag
     1741 cacttgtttg gacacttgtg gacagccatt tacaagaaga gaaccttggt ttagtaagtt
     1801 caatgagtta atggactata ccgatattct attagttgat atcaagcata ttaattcgga
     1861 cgaacataag agattaaccg gattcccaaa tgataatatt ttggatatgt gcgaatatct
     1921 ttctagcata gggaaacctg tttggattcg tcatgtcctt atcccaggca tcacggataa
     1981 tgatgaatac ttgaaacaat tgggcgagta tgttaaggaa aatcttcata atgtcgaaaa
     2041 atttgaagta ttaccttatc atacaatggg cgttcataag tatcatgaaa tgggtatcag
     2101 atatcgtctt gaaggtgttg agcctcctac gccagaaaga gtaaaaaatg ctgaaaaact
     2161 gctagaagta gatagatatc aaggatataa agattggtat ccaggcatta atactttgga
     2221 tcaacaacca agctagttaa attggcaaat acaaatataa aataaaaaca tggtatcgct
     2281 tagtttttac ggttctttgt caaaaagggg ttctttgtca aaaaggactg atgccaaaat
     2341 tttaattatt attttcaa
//
