LOCUS       c00153_gnlPro..         7547 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BFFJEIOL_153.
ACCESSION   c00153_gnlPro..
VERSION     c00153_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 01:24:23
            Original ID  :: gnl|Prokka|BFFJEIOL_153
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7547
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7547
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1831]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1831
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7547
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7547
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1072
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg153_1"
                     /sec_met_domain="Gly_radical (E-value: 1.4e-42, bitscore:
                     133.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.1e-99, bitscore:
                     320.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ENDDVMRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYA
                     INGGVDEKTKQQVGPSYQPITSEYLDYDEVIAKYDQMMDWLAHLYVGTLNMIHYMHDKY
                     YYEAAEMALIDTKVERSFATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDF
                     PRYGNDDDRADEIATTLLSTFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDG
                     RKAGEPLAPGANPSYGAEQNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDER
                     SENLVHVLDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLT
                     REQQLDVIARTCHDRM"
     CDS             1091..1831
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg153_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.2e-29, bitscore:
                     90.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPGGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             2004..4517
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AstE_AspA"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg153_3"
                     /sec_met_domain="AstE_AspA (E-value: 2.6e-33, bitscore:
                     103.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VKDLLSEIESYWTTRAEGYSEVNHKELNGMQKGAWLEVLKGQFPE
                     KAKDEIKILDIGTGPGFFPVILAEAGYKVTAVDYTQEMLDTAKRNAGNLCERISFYKMD
                     AQNLEFEDDVFDVVISRNLTWNLKDPKRAYEEWCRVLKPGGKLLNFDANWYGYLYDEEK
                     RLSYEEDRKSVESEHLDDHYLCTDIDRMEKIALQMPLSSINRPSWDRKFLKENGFESVA
                     VDTGIWQRVWSQEEKLNYHSTPMFMISAVKKEKDIWSENDGTDDSDSRYDRERDLEDAA
                     LCTAPGTKKSGFLKLGGGEFSLPYTVICGSHPGKTVLITAAVHGGEYVGIQAAVELADK
                     LKPEKIHGRVILVKTVCRKEFEERSGSVCPEDEKNLNRVFPGNPNGTRMDRLAYEVVQK
                     LHSAADYYIDLHSGDDYEQLTPYIYYAGCADEDVVQMSRKMAEQADVPYMVKSNVASGG
                     SYNYAAACGIPSVLIERGQMGGWSPEEVHSTRKDVRNILCALGVYDGMRSYSNYYPMEI
                     EDVRYQSASVSGLWYPAKKPGDIIKVGEYLGCIKDYEGNILETSLSDLNGVVLYQTGSL
                     QVIKDGPMITYGSFSRRKDERKEKITNYWAKRSDSFMEQRRAELHSDMADKWQKEIEAF
                     LPDGKLRILDVGCGAGFFSILLAKLGHEVTGIDLTPDMIIHSRELAKEENASCTFEVMD
                     AENPDFPDGTFDVIVSRNLTWTLPDAARAYKEWIRVLKPGGILINADANYGADDFSDTA
                     DLPANHAHFTVGDAMMQECEEIKRQLPISSYVRPAWDLETLGKQGINRFSIDLGISSRV
                     YTKKDEFYNPTPMFLICGEKNKCNN"
     CDS             4554..4652
                     /locus_tag="ctg153_4"
                     /transl_table=11
                     /translation="MESPLFLFCELKMLQKYEKSLTKLIILNIIFL"
     CDS             4712..5548
                     /locus_tag="ctg153_5"
                     /transl_table=11
                     /translation="MKVQKKESKQSGNPVRSLILVTMCTCTAIAFAAGGRIADFNGKNK
                     VYAASENKEKTFSSDADETDVLPTGIAGVVSGMNDTASPKKSVTRIGTSCEQVMVGQRI
                     KVIEDNMAEFDVSSSMESSINELDARTAALAESARMMSDEDYDTLLHIVEAEAGTEDVK
                     GRILVANVIMNRIKNKEFPDTVTEVVWQNTNGVPQFSPTYDGRINEVTVTDETREAVRQ
                     ALEGVDYSEGALFFIQKSEAESQNVSWFEKDLKRLFKYGVHEFYTYPDSAESSTES"
     CDS             5985..7475
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg153_6"
                     /sec_met_domain="PALP (E-value: 2.5e-18, bitscore: 55.1,
                     seeds: 146, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVLYKSTRGKEETVTASMAILKGLSEDGGLFVPTEIPKLDVPMD
                     ELAKMSYQETAYEVMKCFLTDFTEEELKNCINNAYDEKFDTKEIAPLHEADGAYFLELY
                     HGATIAFKDMALSILPHLMTTAAKKNQVKNEIVILTATSGDTGKAAMAGFADVPGTKII
                     VFYPKHGVSPIQEKQMVTQKGNNTYVVGITGNFDDAQTAVKKMFNDKELEAELDTAGYQ
                     FSSANSINIGRLVPQIVYYVYAYASLVRQGKIKDGQEINVVVPTGNFGNILAAYYAKQM
                     GLPVHKLICASNDNKVLYDFFRTGTYDRKRDFILTTSPSMDILISSNLERLIYRIAGGD
                     AKKCAELMQSLTAGGEYTITEDMKAQLADFYGNYCTEDETAKTIAEIFKDSHYVIDTHT
                     AVAAGVYDKYVKDTNDATPTVIASTASPYKFTRSVMEALGADTDGKDDFALADELSALS
                     GVKLPQAVETIRTAPVLHNRVVDAPDMPKAVKDILGIR"
ORIGIN
        1 tgagaacgat gatgttatgc gtccggtatg gggcgatgac tacagcatct gctgctgtgt
       61 atctgctaca gagacaggta aagagatgca gttcttcggc gctcgtgcaa accttgcaaa
      121 atgccttctt tacgcgatca acggcggtgt tgatgagaag acaaaacagc aggtaggacc
      181 aagctatcag ccgatcactt ctgaatatct tgactatgat gaagtaatcg caaaatatga
      241 ccagatgatg gactggctgg ctcatctgta tgtcggaaca ctgaacatga tccactacat
      301 gcatgataaa tactactatg aagctgcaga gatggctctg atcgatacaa aggttgagcg
      361 ttctttcgca acaggtatcg caggattctc tcatgtagta gactccctgt ccgctatcaa
      421 atatgcaaaa gttaaagcta tccgcgatga agacggcatt acaacagatt tccaggttga
      481 gggtgacttc ccacgttatg gtaatgatga tgacagagca gacgagatcg caacaactct
      541 tctgtctaca ttccttgaaa aactgaaaca tatccatact taccgtgatt ccaagccaac
      601 cacatctatc cttactatta cttccaacgt tgtttatggt aaggctacag gatctcttcc
      661 ggatggaaga aaagcagggg aacctcttgc acctggtgca aacccatctt acggtgcaga
      721 gcagaacggc cttcttgctt ccctgaactc tgttgccaag cttgactatg aagatgctct
      781 tgacggtatc tccaatactc agacaatcaa tcctgatgct ctgggacaca gcgatgacga
      841 gcgttcagag aatctggtac atgtacttga cggatacttt gatcagggtg ctcatcacct
      901 caatgtaaac gtatttggca aagagaaact gattgacgct atggaacatc cggaaaaaga
      961 agagtatgca aatttcacaa tccgtgtatc cggatatgct gttaagttca tcgaccttac
     1021 aagagagcag cagttagacg ttatcgccag aacctgccat gacagaatgt aattaatgga
     1081 tgatgatata atggataata aacagataaa aggctatgtt cattctttag aaagcttcgg
     1141 gtccgttgac ggacccggag taagatatgt aattttttta agcggctgtg ccatgagatg
     1201 tcagttctgt cataatccgg atacctggaa gatgaagcag ggagagcttt atacggcaga
     1261 tgagcttctt aagaaagctt tacgttataa gggctactgg ggcagcaaag gcggtatcac
     1321 tgtaagtggt ggtgagccat tgcttcagat ggattttctt acagaatttt ttaagaaggc
     1381 aaaggcagag ggtgtgcata caaatcttga taccagtgga aatcccttca ctgatcagga
     1441 accatggcat tccggatggc tggagcttat gaagtataca gatcttgtaa tgcttgatat
     1501 caagcagatt gacgagcagg agcatattaa acttaccggt cattccaata agaatattct
     1561 tgcgatggcg cgggaacttt ctgatatgaa aaaacctgta tggatccgtc atgtgcttgt
     1621 gccgggcgga agtgataagg atgaatatct gcaccggtta gctgatttta tccatactct
     1681 ttccaatgtg gagagagtgg aggtgctccc ttatcatacg cttggtaagt ttaaatggga
     1741 gaatctgggg ctttcttatc cactggaggg ggttaatcct ccgacgcagg aaaggattga
     1801 taatgcccgg aagattcttg gagctatctg agtaaaaata tatagctgtc atatcctctt
     1861 tttgggggta tggcagcttt gtttttggtt taaaaagggg acgcgaaatg tatcccccgg
     1921 gagggcttgt tagaaagggg agggatttga tataattttg accatatatg attataaatt
     1981 gcatgcatgg aggttggcat aaggtgaagg atttattatc ggagattgag agttactgga
     2041 ctacccgggc tgaggggtat tctgaagtta atcataagga attgaatggg atgcagaagg
     2101 gggcatggct tgaggtgttg aaagggcagt tcccggagaa agcgaaggat gagattaaga
     2161 ttctggatat cggaacagga cccggatttt ttcctgttat tctggctgag gcaggatata
     2221 aagtgactgc tgttgattat acacaggaga tgttggatac tgcgaaaagg aatgcaggta
     2281 atctttgtga acggatttct ttttataaga tggatgcaca gaatctggaa tttgaggatg
     2341 atgtgtttga tgttgtgatc agcaggaatc tgacatggaa tctgaaggat cctaagagag
     2401 cttatgaaga gtggtgtcgc gtactgaaac cgggcggaaa gcttcttaat ttcgatgcga
     2461 actggtatgg ttatctttat gatgaggaga aacgcctgtc ttatgaggaa gaccgaaaga
     2521 gtgtggaaag cgagcatctg gatgatcatt atctttgtac ggatattgac agaatggaga
     2581 agattgcact gcagatgcct ctttcttcta ttaacagacc gtcctgggac aggaagtttc
     2641 tgaaggagaa cggatttgag tctgtggcag tggataccgg gatctggcag cgggtgtgga
     2701 gtcaggaaga gaagctgaat tatcattcta cgccgatgtt tatgatcagc gctgtgaaga
     2761 aagagaaaga tatctggtcc gagaatgacg gaacggatga ttctgatagc agatatgaca
     2821 gggaaagaga tttggaggat gccgcgttgt gtactgctcc gggtacgaaa aagagcggat
     2881 tcctgaaact gggcggaggt gagttttcgc ttccttatac agtaatctgc ggttctcatc
     2941 ccggaaagac agttctgatc accgctgcgg tccatggagg agaatatgtg ggaatccagg
     3001 ctgctgttga gcttgcggat aagctgaaac cggagaagat ccatggaaga gtgattttgg
     3061 tcaagactgt gtgcaggaaa gaatttgagg agaggagcgg cagtgtctgc ccggaggatg
     3121 agaagaatct gaatcgtgta tttccgggga atccgaatgg aaccaggatg gaccgtctgg
     3181 cgtatgaggt ggttcagaag cttcacagtg cagcagatta ttatattgat cttcacagcg
     3241 gggatgatta tgagcagctt acgccttata tttactatgc cggatgtgca gatgaagatg
     3301 tggttcagat gtcaaggaag atggcagagc aggcagatgt tccctatatg gtaaagtcta
     3361 atgtggcatc cggtggttct tacaattatg ctgccgcatg tggcattccc agcgttctga
     3421 tcgaacgtgg ccagatggga ggctggagtc cggaggaggt tcattctaca agaaaagatg
     3481 tgcgtaatat tctgtgtgcg ctgggtgttt acgatggcat gagaagttac agtaattatt
     3541 atcccatgga aattgaagat gtgcgttatc agtctgccag tgtatccggg ctgtggtatc
     3601 ctgccaaaaa accgggagat atcattaagg ttggagagta tcttggctgt attaaggatt
     3661 atgagggaaa tattctggag acaagtttat ccgatttaaa tggagtggtt ctatatcaga
     3721 cgggaagtct tcaggttatt aaggatggtc cgatgatcac atatggcagt ttcagcagaa
     3781 gaaaagatga gcgtaaggaa aagatcacaa attactgggc gaaaagaagc gacagtttta
     3841 tggaacagag aagagctgag cttcacagtg atatggctga taaatggcaa aaagagattg
     3901 aggcttttct tcctgatgga aaattaagga tcctggatgt gggatgcggt gcaggcttct
     3961 tttccattct tttagcaaaa ctgggacatg aagttacagg aatcgatctg acaccggata
     4021 tgatcattca ttccagggaa ctggcaaaag aagagaatgc ctcctgtacg tttgaagtaa
     4081 tggatgcgga aaatccggat tttcctgatg gtacctttga tgtgatcgtg tccagaaatc
     4141 ttacctggac acttccggat gcagcaagag catataaaga atggattcgt gtgctgaaac
     4201 cgggaggaat actgatcaat gcagatgcca attatggtgc ggatgatttc tcagatactg
     4261 ctgatctgcc tgccaatcat gctcatttta cagtaggcga tgccatgatg caggaatgcg
     4321 aggaaattaa acgccagctt cctatcagtt cctatgttcg ccctgcctgg gatctggaga
     4381 cactgggaaa acagggtatc aacagattct ccattgatct gggtatcagc agcagagttt
     4441 atacaaagaa ggatgagttt tataatccaa cccctatgtt cctgatctgc ggcgaaaaaa
     4501 ataaatgtaa taattgagaa aaatgaagca tatattaaag ggagatttca ttgatggagt
     4561 ctcccttgtt tcttttttgc gagctaaaaa tgttacaaaa atatgaaaaa agcttgacaa
     4621 aattaattat attaaatata attttcttat aagttacaga aatgttacaa ccataaaacc
     4681 tgtaattaaa aaaactcaag gaatgataag tatgaaagtt cagaaaaaag agtcaaaaca
     4741 gtccggtaat ccggtgcgga gtctgatact ggttacaatg tgtacctgta cggcgattgc
     4801 gtttgcagca ggcggcagaa tagcagattt taatggaaaa aacaaagtat atgcagcttc
     4861 tgaaaataag gagaaaactt tttcctctga tgctgatgaa acagatgtac tgccaacagg
     4921 aattgcagga gtagtatccg gaatgaatga tacagcttct ccaaaaaaat cagtcacacg
     4981 tatcggaact tcctgtgaac aggtaatggt cggtcagaga attaaagtta ttgaagataa
     5041 tatggcagaa ttcgatgtca gctcttctat ggagagcagc atcaatgagt tggatgcaag
     5101 gacagcagct cttgcagaga gtgcccggat gatgtcggat gaggactatg atacattgct
     5161 tcatattgta gaggctgaag caggtactga ggatgtcaag ggacgcattc tggttgccaa
     5221 tgtgattatg aaccgtataa agaataagga atttcccgat acagtcacag aagtggtatg
     5281 gcagaatacc aatggtgttc cgcagttttc accgacttat gacggacgta tcaatgaagt
     5341 aactgttaca gacgagacaa gagaagccgt cagacaggct cttgaaggtg tggattattc
     5401 agaaggagca ctgtttttta tccagaaatc tgaagctgaa agccagaatg tctcctggtt
     5461 tgagaaagat ctgaaaagac tgtttaaata cggcgtacat gaattttata cctatccgga
     5521 ttcagcagaa agttccacag aatcgtaata aacgatactc ttaatatgac tcaattaaca
     5581 ggattgccat aatactgctc aaaatatgga catattcttc gggagcggaa gaatatgtcc
     5641 atatttttga tttatagtaa tcaatcatag atactgttca ctggcaatgt cccgcgaggt
     5701 gttttttgtg agcgaaggtc acagaaaacc cgagacatac tgcgcgaatt tatgcgatta
     5761 gcataaattt tgtgcatcgc gaagcgtgtt gctgtgaaca gagtgaacag taaccaatca
     5821 taaaaagaca gaaaaaagag aaataattta ggatagtatt ttgctaaagt atatgctata
     5881 atgtgctcaa ttggcgaaat gaataactgt aactgttcag cggtttatta ttactgccaa
     5941 tataccgaac agttacgaat aactttggaa aggcaggaaa tatcatgcag gtattataca
     6001 aaagcacaag aggaaaagaa gaaaccgtaa cagcttctat ggctatttta aaggggcttt
     6061 ctgaggatgg cggcttattt gtgccgacag agattccgaa gctggatgtc ccaatggatg
     6121 agctggcaaa gatgagctat caggagacag catatgaagt aatgaaatgt ttccttactg
     6181 attttacaga agaagaatta aagaactgca tcaacaatgc ctatgatgaa aaatttgata
     6241 caaaagagat cgcaccgctt catgaagcag acggagctta tttccttgag ctgtatcacg
     6301 gtgcgaccat tgcgtttaag gatatggcat tatcaattct tccacatctt atgactacag
     6361 ctgctaagaa gaatcaggta aaaaatgaga ttgttattct taccgctact tccggagata
     6421 caggtaaagc ggcaatggcc ggatttgcag atgttccggg aaccaagatc atcgttttct
     6481 atccgaaaca tggtgtaagt cctatccagg agaaacagat ggtaactcag aaaggcaaca
     6541 atacatatgt tgtgggaatt accggaaact ttgacgatgc acagactgcc gtaaagaaaa
     6601 tgttcaatga taaggaactg gaagcagaac ttgatactgc aggatatcag ttctcatctg
     6661 cgaactctat caatatcgga cgtctggtac cgcagattgt ttattatgta tatgcatatg
     6721 catcacttgt acgtcagggc aagatcaaag acggtcagga gatcaatgtg gtagtaccta
     6781 ccggaaactt tggaaatatc ctggctgctt attatgcaaa acagatggga cttccggttc
     6841 acaaacttat ctgtgcctcc aatgataaca aggttctgta tgatttcttc cgcacaggaa
     6901 cttatgacag aaagcgtgac tttatcctta caacctcacc gtccatggat attctgatct
     6961 ccagcaatct ggaacgtctg atctaccgta tcgcaggcgg tgatgcaaaa aaatgtgcag
     7021 agctgatgca gtctcttact gcaggcggcg agtataccat cacagaagac atgaaggcac
     7081 agcttgcaga cttctatggc aattactgta cagaagacga aacagcaaaa acaatcgcag
     7141 aaatcttcaa agacagtcat tatgtgatag atacccatac tgcagttgca gcaggtgttt
     7201 atgacaaata tgtaaaagat accaatgatg ccacaccaac tgtcattgca tccacagcaa
     7261 gcccatataa atttacaaga agtgtaatgg aagcacttgg agcagataca gacggtaagg
     7321 atgattttgc actggctgat gaactgagtg cgctttccgg agtgaaactt ccacaggcag
     7381 ttgagacgat ccgcacagca ccggtgcttc ataaccgggt ggtagatgcg ccggatatgc
     7441 caaaggcagt aaaagatatc cttggaatcc gataatctcc atattataaa aaatataatt
     7501 aattctacat tttatgttga aaaagaacat atactggagt ataataa
//
