LOCUS       c00077_gnlPro..        16677 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JPEBEHEM_77.
ACCESSION   c00077_gnlPro..
VERSION     c00077_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 04:12:50
            Original ID  :: gnl|Prokka|JPEBEHEM_77
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 16677
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..16677
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3685:6677]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3686..6677
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..16677
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..16677
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             325..1542
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     335.1; E-value: 1.4e-102)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg77_1"
                     /sec_met_domain="Fe-ADH (E-value: 7.9e-103, bitscore:
                     335.1, seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARFTLPRDLYHGKGALEALKSFTGKKAMICVGGGSMKRFGFLDR
                     AVEYLKEAGMEVELFEGIEPDPSVETVMRGAEAMLKFEPDWIIAMGGGSPIDAAKAMWI
                     KYEYPEITFEEMCKVFGIPPLRRKAHFCAISSTSGTATEVTAFSIITDYQKGIKYPIAD
                     FEITPDVAIVDPDLAETMPKKLVAHTGMDAMTHAVEAYVSTAHCDYTDPLAIFAIRMIQ
                     GDLVDSYNGDMSKRDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHIIHGAA
                     NAMYLPKVIAFNAKDPEAKKRYGVIADEMKLGGANDDEKVKLLIEYLRGMNDALNIPHC
                     IQHYGPDSYPTEQGFVPENVFLERLPEIAKNAIADACTGSNPRQPSQEEMEKLLKCCYY
                     DTEVDF"
     CDS             complement(1717..3114)
                     /locus_tag="ctg77_2"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQDLIFPHHENEIAQSECANGCEFARYWMHNGFINVDNQKMSKSLHNFFTVR
                     DVANLYGYEPIRYFMLTAGYRMPLNYTVDLIESCKNSLERLYTCRENLDFALSKTDFGT
                     DETLKEKAAEARTKFCTAMDDDLNTPDALAAVFDLVKEINTLSAASSKAALETAAAAFD
                     EITGVLGLLYNRKKDEVPAEVTALVEKRAAAKKAKDWATADAIRAQLTELGWAVKDTAQ
                     GPQLSKL"
     CDS             complement(3686..4438)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.7;
                     E-value: 1.4e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg77_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.2e-29, bitscore:
                     93.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKALGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQMDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPAYLAAFDRLMANTSLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWVRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEELLEVSWYPG"
     CDS             complement(4425..6677)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg77_4"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-40, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.3e-267, bitscore:
                     878.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQFEQWEGFEGRIWKEEVNVRDFIQKNYTPYDGDESFLAGPTEA
                     TDKLWGALQQLQKAERAKGGVLDMETEVVSSLTSYGPGYIDENLKDLEQIVGLQTDKPL
                     KRAFMPYGGIKMAEQACTTYGYQPSAELHKIFTDYTRTHNQAVFDAYTPEMKKARHTHI
                     ITGLPDTYGRGRIVGDYRRVALYGIDALIQFKQEDLANCGDGTMTDDVIRLREEIARQI
                     SALKGMKKMAEAYGYDISQPAKDAKEACQWLYFGYLAAIKTQNGAAMSVGRISTFLDIY
                     IQRDLDKGILTESQAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVGGIGMDG
                     RSMVTKNCYRFLHTLENMGPAPEPNLTVLYSSALPEAFKKYAAKVSVNTSSVQYENDDV
                     MKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSHEQCGPNYAP
                     ITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMALIDTDVRRTFA
                     TGIAGFSHVIDSLSAIKYAKVKVVRDENGLATGFETEGDFPKYGNDDDRADEIGVWLLK
                     TFLEMIKKRHTYRNSEATTSILTITSNVVYGKYTGALPDGRAAFTPFAPGATPSYGAEQ
                     NGLLASLNSVAKLPYHWALDGISNTQTINPEALGHSEGERVENLVQVLDGYFDQGAHHL
                     NVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVLARTCHGVL"
     CDS             complement(7257..7715)
                     /locus_tag="ctg77_5"
                     /transl_table=11
                     /translation="MKSFHIPRTALFFILFALAAFAVLYRPIDLKTLVRASSQGTLRTE
                     EVVHFGSTNDMDYHLLSMDSEAFQQTAELLSTVSCRKKLNQNYSAYTHDTFPVQSVTVS
                     FYDDAENQKFLLTVYSDGVCILNSTFVSVKYPGGGAAQLYEQLAGMGK"
     CDS             complement(7720..8757)
                     /locus_tag="ctg77_6"
                     /transl_table=11
                     /translation="MSQAAAQQPSRKKAVISLLVLLALTCIIVFTFKDHWVEITAALAQ
                     LSFWQVLAVLAVGLSYPLLEGCVAWVIVRSRLPQFKLWQGLDVGWCGTFGNVVTLGAGA
                     VPVQLYYLHKAGLPLGPGAGLMTLEYVFHKSTVLLYATVMLLLQHRWLAANTTGVMRYL
                     PMAYAVVAVIIVALVLLCVSPLVQNLARWLLGFLPKTEKWQQRRADWLEQLEVLGTESR
                     RLLADKPRCLKIFALQALKLFGLFCLPYLCIRFMGLSPLGFWQVQLLTSLMLFVSNALP
                     NVAGMGSIETAFLLVFGSFLERGEVMSVLMLYRIASYYVVFAASAVGFFIAQRHLTQTE
                     PPKEG"
     CDS             complement(8935..10542)
                     /locus_tag="ctg77_7"
                     /transl_table=11
                     /translation="MYTSYSTLQRKQLSKQVYTDTQSTYLLVYAPGRHKALQAALQNQL
                     HRKFRLVTRLEDALTPDVAGVLLVSEDVECTPTALTYFAAALREGADLAVCDASFGFDG
                     STALYLSTRHLPGSSCALVSRALLDKVRAAARGKDSVTELLRLAHAMAQHSRCIPQALL
                     HFRRELCADDVFSAKGRRALILSHELTMTGAPIVLVSAVPVLRSLGFEVVVLGPSDEGS
                     LPLFLDAGAAVVTRPDCVTSSALWGLATSADLVLANTVVEAPVVNTLNGSFVPVLWWLH
                     DAFAGYPFIAHSIPKTLGKNVHLCAVGSHATAAMHSVRPDFAIEQLIYGLPDYAAEQFP
                     RYDISFAGGRPLFVTVGSLEHRKGPDIFCNAIRLLPSTVREKAAFLFVGKAADKGMYNA
                     VDSLVRDYPKTVFYRKRLERPEVKSLMEQCNCVVCASRDDPMPTFVTEGLIFGKPSIVS
                     EHTGTAGLVTEGVDGFLYKDDDPQQLADKLAWAIEHPEALAAMHGSCRRLYEQQFSNES
                     YVATLTRLVKELTDGEEE"
     CDS             complement(10577..11371)
                     /locus_tag="ctg77_8"
                     /transl_table=11
                     /translation="MNHDAPMTPAAMQAHIAELEQQLKLSDEGVSQLAQRCLELEQQLL
                     ACQTELAKHSAEAENITLTLPQLFYDTGSGFSPRECLTATEDVYNELTHEVSVTFVLPE
                     DARAIRLDPGELACCITDLAISDERISFQSVNGLLLQEDSLLFLDVDPNLSLHCTTGFG
                     AGMKFAVNYHYYPLGRFLHEQPGKSLLRALNDLKLENAAAAQEAAEVLQASRAECVRLN
                     QQLLTLQGIQHEYQVSLETMRASSSWRLTAPLRRLLNLVRGH"
     CDS             complement(11438..12553)
                     /locus_tag="ctg77_9"
                     /transl_table=11
                     /translation="METTKPRTVNSIGMFDMLKGAGMLAIVLGHTAELYPLQLEGGLSL
                     TAFVGFIYRETLMAAFFIASGYGFRKRSISKCIHQQLKSLLKPFCYTAVFTTVLHFIIH
                     YKTFHYLPGSMTESIKVAGGFLLGLPHTATYFGQEFFSCGPMWYLLALLMGWILLDVIL
                     NIFPERYIPWAVGGAALLGWGASLVWELPFCLIQGAVITPYLYLGYLAKRQHWLDQPLS
                     RRMSCILWAAVLLIPAAALATRRTDCVSMAEWSLGPISILLDGLAGLLFIRLFLRWSQR
                     RNPLVRFFEAIGRRSLYIFCVHTVELIAIPWYLFAAKYADRPVLGISLQYILSLGSIWL
                     ICALLQRRRDLIIKLFPARRHTPQVHYAPRH"
     CDS             12929..14398
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 215; E-value:
                     4.6e-66)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg77_10"
                     /sec_met_domain="AMP-binding (E-value: 2.6e-66, bitscore:
                     215.0, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKVLDNVYDLVTLNMEQRFSERVALRFYDKETDEVTAVHYKDYA
                     RDIRRAVSYFQSTIPDIKGKKICLLNKNCYEYAVNTFGIILAGGVLVLLNQHKSWDELS
                     YELGLVDPAAILTDGDDYGTKEQLQAAYGSILRPMDGFRAYEPVAEMPRCIGHDDLMIL
                     MFTSGTTGRSKGVMLSERNFFSVMRAHVQIGERMMEYKHDPELVVSQYTVLPMFHLGAF
                     ICLFSWAHGGWALNISGDIRNFYKEIQRMPSQVMAVVPVIMNSLHKDMMRGRKERLGEL
                     WVPICSSAMFDPQVMLDMATNGMFVVQTYGATETCGDGIINYAQDAKHIGAVGQGNDYL
                     DYKLEPDGELCIRGDSIMLGYYKDPEATAEVIDKDGWFHTGDLARVDEDGYYYITGRKK
                     NLIILDSGENVSPEELEGMLEKCPAVQECIVKELGKKIGVVVYCEKEHQQTVRDFIAQM
                     NRTIPLYKRIGVVEFSETPLPRNATGKLVRK"
     CDS             14426..14668
                     /locus_tag="ctg77_11"
                     /transl_table=11
                     /translation="MERAEIISQITAILEDVAEVSPEDVTESSVLMDDLDLSSMEILTI
                     VADLEETFGLRIPEKELRNFVTMGDLVDYLAANVG"
     CDS             14675..15883
                     /locus_tag="ctg77_12"
                     /transl_table=11
                     /translation="METQQRSTEYNFQGVMYFFRREKIQCRYQFSLRDAVDITLLQRAL
                     TAALASAPYYTQRLVQEKREMWLEPNTEPCLVYPGSTMRNIPEQTNGYLFCVSCEGDTV
                     YFDWHHFLLDGHGVSPLLTSILEQYCNLRYGTAFAVPQIVCDPPYDMEAMLAEYPPVEG
                     GSDAIQRDVVQTYEGALRRTRVCLSKQSLVEKALANNAKPVSALMGLLCMAMREYLGKE
                     KIRYSYSSDTRDVAGVPNALYNCVCSFWHTVQLQAQTRLADFVADVDADIRRDLQPESK
                     RHRMADQMGWVYKVDQQKAPLRIKQRVFQMGEYIGGAISDFWLSYLGNPLMPATPELTQ
                     YTTDFGVWVPPDGASVGVEAASLNGKIILCIHNKAPNPGLAEILRKTFESEGVAVLEAE
                     DLD"
ORIGIN
        1 gagcccgatc cgggtcgcca tttgctgcta tagctcagtt ggtagagcgc atccttggta
       61 aggatgaggt ctccagttcg aatctggata gcagctccaa aggcaagcac ctcgaagtct
      121 taggacttcg gggtgttttt ttgcgctttt tcagggcagg gggcttgttg catcctactg
      181 cttgtgaaaa ccgcccaaaa tagaacgact ttttctcccg gtacgtcaat tgaagctgcc
      241 tccaaattgt tatataatta acataataaa taaaaatccg gcacagcgct gcgtgtgccg
      301 tactattttt ggaggttttg tattatggct cgttttactc tgccccgtga tctgtaccat
      361 ggcaagggcg ctctggaagc gctgaaatcc tttaccggca aaaaggcaat gatctgcgtc
      421 ggcggcggct ccatgaagcg cttcggcttc ctggatcgtg ccgtggagta cctgaaagaa
      481 gccggcatgg aagtggagct gttcgagggc attgaacccg atccttccgt ggagaccgtc
      541 atgcgcggtg ctgaggctat gctgaaattt gagcccgact ggatcatcgc catgggcggc
      601 ggctctccca tcgatgcagc caaggccatg tggatcaagt acgagtaccc cgagatcacc
      661 tttgaggaga tgtgcaaggt gttcggcatc ccgccgctgc gccgcaaggc acacttctgc
      721 gccatttcct ccacctccgg cactgctacc gaggtgactg ccttctccat catcaccgac
      781 taccagaagg gcatcaagta ccccatcgcg gactttgaga tcaccccgga cgtggccatc
      841 gtggaccccg atctggctga gaccatgccc aaaaagctgg ttgcccacac cggtatggac
      901 gccatgactc acgccgtgga ggcttacgtt tccaccgcac actgcgacta caccgacccg
      961 ctggccatct tcgccatccg catgatccag ggcgatctgg tggacagcta caacggcgat
     1021 atgtccaagc gcgactccat gcataacgcc cagtgccttg ccggtatggc tttctccaac
     1081 gcactgctgg gcatcgtgca ctccatggca cacaagaccg gcgctgcttt tgcagactac
     1141 ggcgcacaca tcatccacgg tgctgctaac gctatgtacc tgcctaaggt catcgccttc
     1201 aacgccaagg accccgaggc caagaagcgc tacggcgtca tcgcagacga gatgaagctg
     1261 ggcggcgcaa acgatgacga gaaggtcaag ctgcttatcg agtatctgcg cggcatgaac
     1321 gacgcgctga acatccccca ctgcatccag cactacggcc cagactccta ccccaccgag
     1381 cagggctttg tgccggagaa tgtgttcctg gagcgtctgc ccgagatcgc caagaacgcc
     1441 atcgccgacg cctgcaccgg ctccaacccc cgccagccca gtcaggaaga gatggagaag
     1501 ctgctcaagt gctgctacta cgatactgag gtggatttct gatccgtcct ttctttatta
     1561 cgcctccttc cctatggaca aaagccccgc agagcatacc gccctgcggg gcttttgccg
     1621 ttttcgcctg cattctcccg tgaaaaaggg acaccggagc gttcgcagac gctccggtgt
     1681 cccaaatttt aaaatggttt tccttagcgg caggccttac agcttgctca gctggggacc
     1741 ctgtgcggtg tccttcacag cccagcccag ttcggtcagc tgtgcgcgga tggcatcggc
     1801 ggtggcccag tccttggcct ttttggcggc agcgcgcttc tctaccagcg cggtcacctc
     1861 ggcgggcacc tcgtccttct tgcggttgta cagcagaccc agcacgccgg tgatctcgtc
     1921 aaaggcggcg gcagccgtct ccagtgcagc cttgctggaa gcagcagaca gggtgttgat
     1981 ctccttgacc agatcaaaca cggcagccag cgcatccggg gtgttcaggt cgtcgtccat
     2041 ggcggtgcag aacttggtgc gtgcctcggc agccttttcc ttcagggtct cgtcggtgcc
     2101 gaagtcggtc ttgctcaggg caaagtccag attctcgcgg caggtgtaca ggcgctccag
     2161 actgttcttg cagctctcga tgaggtccac ggtgtagttc aggggcatcc ggtagccggc
     2221 ggtcagcatg aagtagcgga tgggctcgta gccgtagagg tttgccacat cccgcacggt
     2281 aaagaagttg tgcaggctct tggacatttt ctggttgtcc acgttgatga agccgttgtg
     2341 catccagtag cgggcaaact cacagccgtt ggcgcactcg ctctgggcaa tctcgttttc
     2401 gtggtggggg aagatcagat cctgaccgcc gcagtgcagg tcgatggtct tgcccagatg
     2461 ggtgcggctc atggcgctgc actcaatgtg ccagcccggg cggcccatgc cgtaggggct
     2521 ttcccatgcg ggctcaccgg gctttgcagc cttccagacg gcaaagtcgg cgggatcctc
     2581 cttcaaatcg tcctccatgc ggctgcgcag ctcgcggttg ccgctctcca gatcgtccag
     2641 attcaggtgg ctcagcttgc cgtagccggg gtcgctcttc acccggaagt acacatcgcc
     2701 gttcttggca acgtaggcgt gaccggagtc gatcagatcc ttgacgatat ccaaaatctg
     2761 ggggatatgc tcggtagcgc ggggctgcac ggtgggcttt tcgcagttca gaccggcggc
     2821 atccttcagg tactcggcct caaagcgctg cgccacctcc ttcatggagg tgccctcttc
     2881 ctgtcccttc ttgatgagct tatcgtcgat atcggtgatg ttggaaacgt agatcacctt
     2941 gttgccccgg tattccagat agcggcgcag ggtgtcgaac acgatcagcg ggcgtgcgtt
     3001 gccaatgtgg atatagttgt acacggtagg gccgcagacg tagattcggt actcgccggg
     3061 cacctgcggc acaaattctt ccttctgtcg ggtcagggtg ttgaagatct gcatggtcaa
     3121 ttctcctttt tgttccaaac agaagccgca aaacaaaacg cccccggagc ggtgcacaag
     3181 gcacaactcc gaaggcggtt ttaaaatcaa aatcgcggtt ccacttctgt ttgtcgctca
     3241 aagccggtaa cggcggcgca ccgcacggcg atacttcccc acggggttcc cgccgcgtgc
     3301 tgtccgggcg cactttgcgc ggcggtctgc aaacaggctt ccagccagtg gcctgttctc
     3361 tctgcgcaga cggtgtgcgg cgctactctg cccggataaa cgcatttatc cttttacccc
     3421 tttattatag cggtgcggtg cagggaagtc aaggtaaaac gaagaacgat tcagaatgcc
     3481 cgccgcgttg ctttgggcat cttcagcgga agaattattt taaatttcgg gatatcggaa
     3541 cgcctgcggg cgttccgata tcccattttt acccgtgaaa agggcccccg gagcgtccgc
     3601 agacgctccg ggggccgaaa tcatagatat tctttccgct gaaattgcgc agagcgaagc
     3661 ggagcgcaat taaaatggtc gagctttacc cgggatacca gctcacttcc agcagttctt
     3721 ctgcccgctt tacctgctct gcggtgggcg gtacagcgtc cggcagggga taggggatgc
     3781 ccagcttctg ccacttgaac agccccaagg tgtggtaggg cagcacctcc acccgctgca
     3841 ccgttttcag gctgcggatg aagtctgctg ttttgcgcag cccttcctcg tcgtcggtca
     3901 gaccgggcac aaggacgtgc cgcacccaca gcgggatgcc gaggtcggag atgtgtcgcg
     3961 ccatggcaag gatgttggcg ttgtccttgc cggtgagctg ccggtggcgc tccgggtcga
     4021 tctccttgag gtcaaggatc accagactgg tgttagccat caggcggtca aatgcggcaa
     4081 gatacgccgg gtcgtccggg cggaagggct gcccggcggt gtccagcgcc gtgtgcacgc
     4141 ccttggcacg cgctagcgtg aagaactcag tcaggaaatc catctgccgc agcggctcgc
     4201 cgccgctgac cgtgatgccg ccctttttgc cccagtagtt gcggtagcgg tatacccgct
     4261 ggaacagcgc ttccgccgtc cacgcctcgc cgccctctgc ccatgtttcg gggttgtggc
     4321 agtacttgca ccgcagggca cacccctgca aaaacaccac aaagcgcacg ccggggccgt
     4381 ccaccgagcc gaaggattcc agcgagtgga cgtatccaag ggccttacag gacgccatgg
     4441 caggtgcgtg ccagcacgtc cagctgctgc tcgcgggtca ggtcgatgaa cttgacggcg
     4501 tagccggaaa cgcggatggt gaagttggcg tactcttcct tctcggggtg ctccatggcg
     4561 tccagcagct tctccttgcc aaagacgttc acgttcaggt ggtgtgcgcc ctgatcgaag
     4621 tagccgtcca gcacctgcac cagattctcc acgcgctcgc cctcgctgtg acccagagcc
     4681 tcggggttga tggtctgggt gttggagatg ccgtccagtg cccagtggta gggcagcttt
     4741 gccacggagt tcagcgaggc cagcaggccg ttctgctctg caccgtagct gggggtagca
     4801 ccgggcgcaa acggggtgaa ggcggcgcgg ccatccggca gagcgccggt gtacttgccg
     4861 tacaccacgt tggaggtgat ggtcaggatg gaggtggtag cctcggagtt gcggtaggtg
     4921 tggcgcttct tgatcatctc aaggaaggtc ttgagcagcc acacgccgat ctcgtcggcg
     4981 cggtcgtcat cgttgccgta cttggggaag tcgccctcgg tctcaaagcc ggtggcaagg
     5041 ccgttttcat cgcgcaccac cttcaccttg gcgtacttga tggcgctcag ggagtcgatg
     5101 acgtgggaga agcctgcaat gccggtggca aaggtgcggc gcacatcggt gtcgatgagt
     5161 gccatctcgg cttcctcgta gtagtacttg tcgtgcatat actggatgag gttcagcacg
     5221 ttgacgtaca gccctgccag ccagtccagc atctgcacat acttgggcag cacctcgtca
     5281 taggtcaggt actcgctggt gatgggggcg tagttggggc cgcactgctc gtggctcttc
     5341 tcgtccacac cgccgttgat ggcgtacagc aggcacttgg caaggttggc gcgtgcgccg
     5401 aagaactgca tctccttgcc ggtctgggtg gcagacacgc agcagcagat ggagtaatcg
     5461 tcgccccaga cgggcttcat cacgtcatcg ttctcgtact ggacgctgct ggtgttgacg
     5521 gacaccttgg cggcgtactt cttaaaggcc tcgggcaggg cagaggagta cagcacggtc
     5581 aggttgggct ccggtgcggg gcccatgttc tccagcgtgt gcaggaagcg gtagcagttc
     5641 ttggtgacca tgctgcggcc gtccatgccg atgccgccca cctccagggt tgcccacacg
     5701 gggtcgccgg agaacagctg gttgtagctg gggatgcggg caaacttgac catgcggaac
     5761 ttcatgacca tgtggtcgat gagctcctgt gcctgacttt cggtcaggat gcccttgtcc
     5821 agatcgcgct ggatataaat gtcaaggaag gtggagatgc ggcccacgct catggcggcg
     5881 ccgttctggg tcttgatggc ggccagatag ccgaagtaca gccactggca ggcctccttg
     5941 gcgtccttgg caggctggga gatgtcgtag ccgtaggcct cggccatctt cttcatgccc
     6001 ttcagggcgc tgatctggcg ggcgatctcc tcgcgcagac ggatgacatc gtcggtcatg
     6061 gtgccatcgc cgcagttagc aagatcctcc tgcttgaact ggatcagggc atcgatgccg
     6121 tacagggcaa cgcggcggta gtcgcccacg atgcggccgc ggccgtaggt gtccggcaga
     6181 ccggtgatga tgtgggtgtg gcgtgccttc ttcatctcgg gggtgtaggc atcgaacaca
     6241 gcctgattgt gggtgcgggt gtagtcggta aagatcttgt gcagctcggc agagggctgg
     6301 tagccgtagg tggtgcaggc ctgctctgcc atcttgatgc cgccgtaggg cataaaggca
     6361 cgcttgaggg gcttgtcagt ctgtaagcca acgatctgct ccagatcctt caggttttcg
     6421 tcaatatagc cggggccgta ggaggtcagg ctgctgacca cctcggtctc catatccagc
     6481 acgccgccct tggcgcgctc ggccttctgc agctgctgca aagcacccca aagcttatcg
     6541 gtagcctcgg tggggcctgc cagaaagctt tcgtcgccgt catagggggt gtagtttttc
     6601 tggatgaagt cacgcacatt gacctcttcc ttccagatac ggccttcaaa gccttcccac
     6661 tgttcaaact ggatcattgg aacctccttg ctccattatg cggttagcat tttctaacta
     6721 atgagccttt gaaaaaccgc tctgcatagg cggctttctg ggggcgaaac ggtcttgaac
     6781 tgccggctgc ggcagccctg aatacaaggt gtagacaaaa ataaaaacct gtccacaaaa
     6841 tcttgcgtta ctataatagc aagaaccgtt ttaaaaagca atagaaaaca agtgggtttt
     6901 cttccagtgg aaaggctgtt ctttgggcag aatgtagaaa aaacgccgaa accccggcgg
     6961 cacggcatct ttgcgaaaaa atttgcaaat taagggttga caagactaac tgactgtggt
     7021 atcctgttct cgcagcggtg gttagctaat tctaaccacg ttgcaccaga aaaaaggagc
     7081 gtctgcaagc gctccgctcc attcctccat tcctttctct tttctatggg aagcggcagc
     7141 acggtttgct ctgctgcttc cggcgcggca ccggctgtgt ttggtaccac agccgacgca
     7201 aagaaaacaa aaacgggaca ctcagcttga ccacctgagt gccccgattt ttgtttttac
     7261 ttccccatcc ccgccagctg ttcatacagc tgcgctgcgc cgccgccggg gtattttacg
     7321 ctgacaaagg tgctgttcag gatgcacacg ccgtcggagt acaccgtcag cagaaacttc
     7381 tggttctcgg catcgtcata aaagctgacg gtgaccgact gcaccgggaa ggtatcgtgg
     7441 gtgtaggcag aatagttctg gttcagtttt ttgcggcagg agacggtgga aagcaattct
     7501 gccgtttgtt ggaacgcttc actgtccatg gagaggaggt gatagtccat gtcgtttgtg
     7561 ctgccaaaat gcaccacttc ctcggtgcgg agcgtgccct gcgagctggc gcgtaccagt
     7621 gttttcaggt cgatggggcg gtacagcacc gcaaaagctg ccagtgcaaa caggataaaa
     7681 aataaagcgg tgcgtggaat gtggaagctt ttcatcggct cacccctcct ttggcggctc
     7741 cgtttgtgtc aaatgccgct gggcgatgaa aaagcccacg gcgctggcgg caaataccac
     7801 ataatagctg gcaatgcggt agagcatcag cacgctcatc acctcgcccc gctccagaaa
     7861 gctgccgaac accagcagaa aggcggtttc aatcgagccc atcccggcta cgttgggcag
     7921 ggcgttggag acgaagagca tcaggctggt gagcagctgc acctgccaga agcccagtgg
     7981 ggaaagcccc atgaaacgga tgcacaggta gggcaggcag aacagcccga acagcttgag
     8041 cgcctgcagg gcaaaaattt tcaggcagcg gggcttatct gccagcaggc ggcggctctc
     8101 ggtgcccagc acctccagct gctccagcca gtcggcgcgg cgctgctgcc acttttcggt
     8161 cttgggcaga aagcccagca gccagcgggc gaggttctgc accagcggcg agacgcacag
     8221 cagcaccagc gccacgataa tcaccgctac cacagcgtat gccatgggca gatagcgcat
     8281 gacaccggtg gtgttggcgg caagccagcg gtgctgcaac agcagcatca cggtggcgta
     8341 caaaagcacc gtgctcttgt ggaaaacgta ttccagcgtc atcagccctg cgccggggcc
     8401 cagcggcagc ccggccttgt gcaggtagta cagctgcacc ggcaccgcac cggcacccag
     8461 cgtgaccacg ttgccaaagg tgccgcacca gcccacatct aacccttgcc acagcttgaa
     8521 ctgcggcaga cggctgcgca cgatgaccca cgccacgcac ccctccagca gggggtagct
     8581 gaggccgacc gccagcaccg ccagcacctg ccaaaacgac agctgcgcca gcgccgcagt
     8641 gatctcgacc cagtgatcct taaaagtgaa cacgatgatg caggtaagtg ccagcagcac
     8701 cagcagagag atcacggcct ttttgcggga gggctgctgc gcggcggctt ggctcatgga
     8761 aacaaaactc ctttcaaaac cggaaattct ggaaccagta tagcacatct cccgccgccc
     8821 tttgtgaacg gatgcggaac tttgttttgc aatttggggg atttgataaa atggggagac
     8881 ctctcccgtg aaaaagcaat gccgggcagc gtgcagctgt ccggcattgt tttttcattc
     8941 ttcttccccg tctgtcagtt cctttaccag ccttgtcagg gtagccacat aggattcgtt
     9001 ggagaactgc tgctcgtaca ggcggcggca gctgccgtgc atggcggcca gcgcctccgg
     9061 gtgctcgatg gcccaggcca gcttgtcggc cagctgctgc gggtcgtcgt ccttgtagag
     9121 gaagccgtcc acgccctcgg tcacaagccc ggcggtgccg gtgtgctccg atacgataga
     9181 aggcttgccg aagatcagtc cctcggtgac gaaggtgggc atggggtcat cccgggaggc
     9241 gcacaccacg cagttgcact gctccatcag ggattttacc tccgggcgct ccagccgttt
     9301 gcggtagaaa acggtcttgg gatagtcccg taccaagctg tccacggcgt tgtacatccc
     9361 cttgtcggcg gctttgccca caaaaaggaa ggcggctttt tcccgcactg tagagggcag
     9421 cagccggatg gcgttgcaga agatgtccgg acccttgcgg tgctccagcg agcctaccgt
     9481 cacaaagagg ggacgccccc cggcaaagct gatgtcatag cgggggaatt gctcggctgc
     9541 atagtccggc agaccgtaga tcagctgctc gattgcaaaa tccggccgga cggagtgcat
     9601 ggcggcggtg gcgtgggagc ctacggcgca gaggtgcacg tttttgccca gcgtcttggg
     9661 gatgctgtgg gcgatgaagg ggtagcctgc aaaggcatcg tgcagccacc acagcaccgg
     9721 cacaaaggag ccgttcaggg tgttcaccac cggcgcttcc accactgtgt tggccagcac
     9781 aaggtctgcg ctggtggcca gcccccacag ggcgctggaa gtaacgcagt ccgggcgggt
     9841 gaccacagcg gccccggcgt ccagaaacag cggcagcgag ccttcatccg agggccccag
     9901 caccaccacc tcaaagccta agctgcgcag caccggcacg gcgcttacca gcacgatggg
     9961 cgcgccggtc atggtcagct catggctcaa aatcagcgca cggcggccct tggcagagaa
    10021 cacatcgtcg gcgcacagct cccggcggaa gtgcagcagc gcctgcggga tgcagcggct
    10081 gtgctgcgcc atggcgtggg ccagacgcag cagctcggta acgctgtcct tgccccgggc
    10141 ggcggcgcgt accttgtcca gcaatgcgcg ggacaccagt gcgcagctgc tgccgggcag
    10201 gtgccgggtg ctcaggtaca gggcggtgga gccgtcaaag ccaaaggagg cgtcgcagac
    10261 cgcaaggtct gcgccctccc gcagggcggc ggcaaaatag gtcagggcgg tgggagtgca
    10321 ctccacatcc tcgcttacca gcagcacccc ggctacatcg ggggtcagcg cgtcctcaag
    10381 ccgggtcacc agacggaact tgcggtgcag ctggttttgc agcgccgctt gcagcgcctt
    10441 gtgacgcccc ggcgcgtaga ccagaagata ggtgctctgg gtatcggtat agacctgctt
    10501 ggacaattgc ttgcgctgca gggtactgta actggtgtac atgatccaac tcctccgggc
    10561 agaatatcgg gcgggctcag tgaccgcgca ctaggttcag cagtctgcgc agcggggcgg
    10621 tcagccgcca gctgctgctg gcgcgcatcg tttccagcga aacctgatat tcgtgctgga
    10681 tgccctgcaa ggtcagcagc tgctggttca gccgcacaca ctcggcgcgg ctggcctgca
    10741 atacttccgc tgcctcctgc gcggcagcgg cgttctcaag cttcaggtcg ttcagcgccc
    10801 gcagcagaga cttgccgggc tgctcgtgca gaaaacgtcc cagcgggtag tagtgatagt
    10861 tcaccgcaaa cttcatgccg gcaccaaagc cggtggtgca gtgcagcgaa aggttggggt
    10921 ctacgtccaa aaacagcagg ctgtcctcct gcagcagaag gccgttgacc gactggaagc
    10981 tgatgcgctc gtccgagatg gcaaggtcgg tgatgcagca ggccagctcg ccggggtcca
    11041 gacggatggc gcgtgcgtcc tccggcagga caaaggtgac cgagacctcg tgggtcagtt
    11101 cgttgtagac atcctcggtg gcagtcagac attcccgggg cgaaaagccg ctgccggtgt
    11161 cgtagaacag ctgcggcagg gtaagggtga tattctcagc ctcggcgctg tgcttggcaa
    11221 gctcggtctg gcaggccagc agctgctgct ccagctccag acaccgctgc gccagctggg
    11281 aaacaccctc gtccgaaagc ttgagctgct gctccagctc ggcaatgtgc gcctgcatgg
    11341 cagcaggggt catgggggca tcgtggttca taacgggatc ctcctcttcc tttatcctat
    11401 aacagctgca ccgccgcagc caaaacattc agaaaaatca gtgccgcggg gcgtaatgta
    11461 cctgtggggt gtgccgccgg gcggggaaca acttgatgat cagatcccgc cgccgctgca
    11521 gcagggcgca gatcagccag atggagccca gcgaaaggat gtattgcagg ctgatgccca
    11581 aaacagggcg gtcggcgtat ttggcggcga acagatacca cggaatggcg atcagctcca
    11641 ccgtatgtac gcagaagatg tacagggaac ggcggccgat ggcctcgaaa aagcgcacca
    11701 gcgggttgcg gcgctggctc cagcgcaaaa acagccggat aaacagcaga ccggcaaggc
    11761 cgtccagcag gatgctgatg gggcccagcg accactcggc catggataca cagtcggtgc
    11821 ggcgggtggc aagggcggct gccgggatca gcagtacggc tgcccacagg atgcaggaca
    11881 tccggcggga caaaggctga tccagccaat gctgccgttt ggcaaggtag ccaaggtaca
    11941 ggtacggcgt gatcaccgcg ccctggatca ggcagaaggg cagctcccat acaaggctgg
    12001 caccccagcc cagcagggcg gcaccgccca ccgcccaggg aatgtaccgc tccgggaaga
    12061 tgttcaggat aacgtccagt aagatccagc ccatcagcag cgccagcaga taccacatgg
    12121 gcccgcagga aaagaactcc tgcccgaagt aggtggcagt gtggggcaga cccagcagaa
    12181 agccgcccgc cactttgatg ctttcagtca tggagccggg cagatagtga aaggttttat
    12241 aatgaatgat gaagtgcagc accgtggtga acaccgcagt gtagcaaaag ggcttgagca
    12301 ggcttttgag ctgctggtgg atgcacttgc tgatggagcg cttgcggaag ccgtagccgc
    12361 tggcaataaa aaaggccgcc atcagcgttt cgcggtagat aaagccgaca aaggccgtca
    12421 gcgacaggcc gccctccagc tggagtgggt aaagctccgc agtgtgtccc agcacgatgg
    12481 ccagcatccc agcgcccttg agcatatcaa acatcccgat ggagttgaca gtgcggggct
    12541 tggttgtttc catgaaagat tccttccctg cgccgggcta atacccctcg agaaaaaagt
    12601 cggatacggc gcaaatgatc agtttttata cagcattgcc atgatatatg gctagtataa
    12661 tgctttttat agagactgtc aattttgttt tgcagtctgc accataccgg aactggtatt
    12721 ttgcgctaaa aattgtagca cgaaaaccgg cggattgcaa cgaaaggtag aatttggccg
    12781 gggcgtatcc caagatgtgc aattgaaagt tgtcaaagta tgaaattaaa tgttgcagaa
    12841 gtattgcatt gcattggaag atattgtata ctgagcacag cgcggcgcac tgccgcagga
    12901 tgaaataaca acgcaggagg aaaatacaat gatcaaggta ttggacaacg tgtatgatct
    12961 ggtcaccctg aatatggaac agcgtttttc ggagcgtgtg gcgctccgct tttatgataa
    13021 ggaaacggac gaggtgaccg cagtccatta taaggactac gcacgggaca tccgccgtgc
    13081 ggtcagctac ttccagagca ccatcccgga tatcaagggc aaaaagatct gcctgctgaa
    13141 caagaactgc tacgagtatg ccgtgaacac cttcggcatc attctggcgg gcggtgtgct
    13201 ggtactgctc aaccagcaca agagctggga cgagctgtcc tacgagctgg ggctggtgga
    13261 ccccgctgcc atcctgaccg acggtgacga ctacggcaca aaagagcagc tgcaggccgc
    13321 ctacggcagc attctgcgcc ccatggacgg cttccgcgct tatgagccgg tggcagagat
    13381 gccccgctgc atcgggcacg acgacctgat gatcctgatg ttcacctccg gcaccaccgg
    13441 ccgcagcaag ggtgtgatgc tgagcgagcg caacttcttc agcgtgatgc gcgcccatgt
    13501 gcagatcggt gagcgcatga tggagtataa gcacgacccg gagcttgtgg tcagtcagta
    13561 cacggtgctg cccatgttcc atctgggcgc attcatctgc ctgttctcct gggcacacgg
    13621 gggctgggcg ctgaacatca gcggcgatat ccgcaatttt tataaggaga tccagcggat
    13681 gcccagtcag gtcatggcgg tggtgccggt gatcatgaac tccctgcaca aggacatgat
    13741 gcgcggccgc aaggagcgct tgggcgagct gtgggtgccc atctgctcct ctgccatgtt
    13801 tgacccgcag gtgatgctgg atatggccac caacgggatg ttcgtggtgc agacctacgg
    13861 cgctaccgag acctgcggcg atggcatcat caactacgcg caggatgcaa agcacatcgg
    13921 cgctgtggga cagggcaacg attatctgga ctacaagctc gagccggacg gagagctgtg
    13981 catccggggc gacagcatca tgctgggcta ctataaggac cccgaggcca ccgcagaggt
    14041 catcgacaag gacggctggt tccacaccgg cgaccttgcc cgggtggatg aagacggcta
    14101 ctactacatc accggccgca agaagaatct gatcattctg gacagcggcg agaacgtcag
    14161 tcccgaggag ctggagggta tgctggaaaa atgccctgcc gtacaggaat gtatcgtaaa
    14221 agagctgggc aaaaagatag gtgttgtggt atactgtgaa aaggagcacc agcagaccgt
    14281 gcgggacttt attgcccaga tgaaccgcac catcccgctg tacaagcgca tcggcgtggt
    14341 ggagttcagc gaaacgccgc tgccccgcaa cgctacgggc aaactggtgc gcaagtaaaa
    14401 cgcaacagaa aggaagcata atacgatgga acgtgcagaa atcatttctc agatcacggc
    14461 tattctggag gatgtcgcag aggtatcgcc ggaggatgtg accgaaagca gcgtcctgat
    14521 ggacgatctg gatctgtcct ctatggagat cctgaccatt gtggcagatc ttgaggagac
    14581 ctttggtctg cgcatcccgg aaaaggagct gcgcaacttt gttaccatgg gtgatctggt
    14641 ggattatctg gcagcaaacg tggggtaaaa accaatggaa acacaacagc gctcgacgga
    14701 atataacttt cagggagtca tgtacttttt ccgtcgggaa aagatccagt gccggtatca
    14761 gttctcgctg cgggatgcgg tggatatcac ccttttgcag cgggcactga cagcggcctt
    14821 ggcaagtgcg ccttattata cccagcgtct ggtgcaggaa aagcgggaga tgtggctgga
    14881 gcccaatacg gagccctgcc ttgtgtaccc cggcagcacc atgcgcaata tcccggaaca
    14941 gacgaacggc tacctgttct gcgtcagctg cgaaggggat accgtctact ttgactggca
    15001 tcacttcctg ctggacggcc acggggtatc gcccctgctc accagcattc tggagcagta
    15061 ctgcaacctg cgttacggta cggcctttgc ggtgccgcag atcgtgtgcg acccgcccta
    15121 tgatatggag gccatgctgg cagagtaccc gcctgtggag ggcgggagcg acgccatcca
    15181 gcgggacgtg gtgcagacct acgagggtgc gctgcgccgc acccgggtgt gcctgagcaa
    15241 gcagagcctt gtggaaaaag cgctggcgaa caacgcaaag ccggtaagcg ctctgatggg
    15301 tctgctgtgt atggccatgc gggagtatct gggcaaggag aagatccggt attcctactc
    15361 ctccgatacc cgcgatgtgg ccggtgtgcc gaatgcgctg tacaactgcg tatgcagctt
    15421 ttggcatacg gtgcagctgc aagcccagac ccgactggca gattttgtgg ctgatgtaga
    15481 tgcagacatc aggcgggacc tgcagccgga gtccaagcgt caccgtatgg ccgaccagat
    15541 gggctgggtg tacaaggtgg accagcaaaa agcccccctg cgcatcaagc agcgggtgtt
    15601 ccagatgggc gagtacatcg gcggcgctat ttcggacttc tggctcagct atctgggcaa
    15661 tccgctcatg cccgccaccc cggagctgac acagtacacc acggattttg gcgtctgggt
    15721 accgccggac ggtgcatccg tgggcgtgga agccgccagc ctgaacggta agatcatcct
    15781 gtgcatccac aacaaggcac ccaacccggg tctggccgaa atcctccgca agacctttga
    15841 aagcgaaggc gttgcggtgc tggaagccga agatctggac tgagaaatac ttatcataaa
    15901 agaaaaaact gctgcacgtt ttgtgcggca gtttttttgc tgctttttgt aagaaaatcc
    15961 ggttgcaaag atgcaaaaaa atggttaaat agaaaaaagg atatttccgc aaggagagga
    16021 acaccatgga acaacagaac acgcgcctgc gcaatgtggg ctttgcgacc tttttcttca
    16081 gcggcatctg cgccatcagc gcgggtgtgg tggtcagtct gctgcaggag cgctacggct
    16141 ttgcctacgg catgaccggt acgctgctct ccctgatgag cgtgggcaac ctgctggcgg
    16201 ggctgctgat gggcatgctg cccagtgcgc tgggcatgaa acgcagtgtg ctgctgctga
    16261 ccatcggcta tgcggcgggc tacgccgtca tgggactgac cggcgcagtg gcgctgctgg
    16321 cagcggcctt ttttgtggta ggcatcgcta agggcagcgt tatcaacacc tgtaccattc
    16381 tggtcagcga tcactctgcc gaccgcaccc ggggcatgaa cctgatgcac agctgctacg
    16441 cctgcggcgc actgctgtgc cccttcctca ttgcggcggc agcccgggta agcaccgagc
    16501 tggcggtctt tctgctggca gccgtgggcc ttgtgctctg gctggtgtat gtctttaccc
    16561 cgctgcgcgg aggcaagagt aaaagcaacg cagagaaaga agccatcgac tggagctttc
    16621 tgcgctctgc ccgtttctgg ctgctgaccg gattgctctt tttccagaat gccgccg
//
