GCA_014863975.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaPKAANBCB_1 (original name was: gnl|Prokka|PKAANBCB_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	ranthipeptide	16,708	38,315
Region 1.2	RRE-containing	139,586	159,849

gnlProkkaPKAANBCB_30 (original name was: gnl|Prokka|PKAANBCB_30)

Region	Type	From	To	Most similar known cluster		Similarity
Region 30.1	HR-T2PKS,NRPS	1	71,748	leucomycin	Polyketide	3%

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	ranthipeptide	16,708	38,315
Region 1.2	RRE-containing	139,586	159,849
Region 30.1	HR-T2PKS,NRPS	1	71,748	leucomycin	Polyketide	3%

No secondary metabolite regions were found in these records:: gnlProkkaPKAANBCB_2 (original name was: gnl|Prokka|PKAANBCB_2); gnlProkkaPKAANBCB_3 (original name was: gnl|Prokka|PKAANBCB_3); gnlProkkaPKAANBCB_4 (original name was: gnl|Prokka|PKAANBCB_4); gnlProkkaPKAANBCB_5 (original name was: gnl|Prokka|PKAANBCB_5); gnlProkkaPKAANBCB_6 (original name was: gnl|Prokka|PKAANBCB_6); gnlProkkaPKAANBCB_7 (original name was: gnl|Prokka|PKAANBCB_7); gnlProkkaPKAANBCB_8 (original name was: gnl|Prokka|PKAANBCB_8); gnlProkkaPKAANBCB_9 (original name was: gnl|Prokka|PKAANBCB_9); gnlProkkaPKAANBCB_10 (original name was: gnl|Prokka|PKAANBCB_10); gnlProkkaPKAANBCB_11 (original name was: gnl|Prokka|PKAANBCB_11); gnlProkkaPKAANBCB_12 (original name was: gnl|Prokka|PKAANBCB_12); gnlProkkaPKAANBCB_13 (original name was: gnl|Prokka|PKAANBCB_13); gnlProkkaPKAANBCB_14 (original name was: gnl|Prokka|PKAANBCB_14); gnlProkkaPKAANBCB_15 (original name was: gnl|Prokka|PKAANBCB_15); gnlProkkaPKAANBCB_16 (original name was: gnl|Prokka|PKAANBCB_16); gnlProkkaPKAANBCB_17 (original name was: gnl|Prokka|PKAANBCB_17); gnlProkkaPKAANBCB_18 (original name was: gnl|Prokka|PKAANBCB_18); gnlProkkaPKAANBCB_19 (original name was: gnl|Prokka|PKAANBCB_19); gnlProkkaPKAANBCB_20 (original name was: gnl|Prokka|PKAANBCB_20); gnlProkkaPKAANBCB_21 (original name was: gnl|Prokka|PKAANBCB_21); gnlProkkaPKAANBCB_22 (original name was: gnl|Prokka|PKAANBCB_22); gnlProkkaPKAANBCB_23 (original name was: gnl|Prokka|PKAANBCB_23); gnlProkkaPKAANBCB_24 (original name was: gnl|Prokka|PKAANBCB_24); gnlProkkaPKAANBCB_25 (original name was: gnl|Prokka|PKAANBCB_25); gnlProkkaPKAANBCB_26 (original name was: gnl|Prokka|PKAANBCB_26); gnlProkkaPKAANBCB_27 (original name was: gnl|Prokka|PKAANBCB_27); gnlProkkaPKAANBCB_28 (original name was: gnl|Prokka|PKAANBCB_28); gnlProkkaPKAANBCB_29 (original name was: gnl|Prokka|PKAANBCB_29); gnlProkkaPKAANBCB_31 (original name was: gnl|Prokka|PKAANBCB_31); gnlProkkaPKAANBCB_32 (original name was: gnl|Prokka|PKAANBCB_32)

Compact view

gnlProkkaPKAANBCB_1 - Region 1 - ranthipeptide

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Location: 16,708 - 38,315 nt. (total: 21,608 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaPKAANBCB_1 - Region 2 - RRE-containing

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Location: 139,586 - 159,849 nt. (total: 20,264 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaPKAANBCB_30 - Region 1 - HR-T2PKS,NRPS

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Location: 1 - 71,748 nt. (total: 71,748 nt) Show pHMM detection rules used

Region on contig edge.

HR-T2PKS: (hr-t2pks-ksa and (t2fas or APE_KS2 or ketoacyl-synt))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 71748:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 24416 - 71748:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg30_44

Search NORINE for peptide: strict or relaxed

AMP-binding (457..845): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASFLGGVYK
Nearest Stachelhaus code(s):: Ile DALFMGGVYK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1479..1869): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMKNLGTVSK
Nearest Stachelhaus code(s):: Asp DMKDLGMVDK (53% 8Å match)
Stachelhaus code match:: 70% (weak)