LOCUS       c00088_gnlPro..         6308 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NGKEMLHH_88.
ACCESSION   c00088_gnlPro..
VERSION     c00088_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 02:55:42
            Original ID  :: gnl|Prokka|NGKEMLHH_88
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6308
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6308
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[680:6003]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      681..6003
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6308
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6308
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..317)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HI0933_like"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg88_1"
                     /sec_met_domain="HI0933_like (E-value: 7.1e-26, bitscore:
                     79.6, seeds: 46, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VTNACDTEELFPAMMSNPKFLYSSFYSFTPQDVMEFFEKAGVPLK
                     VERGNRVFPQSDHSSDIIRALERELKKAGAKIHLHTAVQEIVKKPVTESVTDSVNTLES
                     E"
     CDS             complement(363..569)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HI0933_like"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg88_2"
                     /sec_met_domain="HI0933_like (E-value: 7.6e-11, bitscore:
                     30.1, seeds: 46, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMPEVHIYIDRSEAETWNHDEIDNLQGKINTGEYSMSKVIIIGGG
                     AAGMMAGVFAARNHHEVHILEKN"
     CDS             complement(681..2093)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_3"
                     /sec_met_domain="HMGL-like (E-value: 3.6e-47, bitscore:
                     150.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 2.8e-59, bitscore:
                     189.1, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELEKKPVKIVETILRDAHQSQIATRMTTEQMLPIVDKLDKVGY
                     HAVECWGGATFDASLRFLHEDPWERLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVVE
                     YFVQKSAANGIDIIRIFDCLNDLRNLQTAVSAANKEKAHAQVALSYTLGDAYTMEYWTD
                     IAKRIEDMGADSICIKDMAGLLLPNKATELVTALKETVKIPIDLHTHYTSGVASMTYLK
                     AVEAGVDIIDTAMSPFALGTSQPATEVMVETFKDTPYDTGFDQKLLSEIADYFRPIRDE
                     ALDSGLLNPKNLGVNIKTLLYQVPGGMLSNLTSQLKEQHAEDKFYDVLEEVPRVRKDLG
                     EPPLVTPSSQIVGTQAVFNVLMGERYKMVTKETKDILLGKYGATVKPFNPEVQKKCIGD
                     EKPITCRPADLLDNELEKLESEMAQYKEQDEDVLTYALFPQVAMDFFKYRQAQKTKVDE
                     KAADKKNGAYPV"
     CDS             complement(2236..3384)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_4"
                     /sec_met_domain="OAD_beta (E-value: 2.9e-157, bitscore:
                     511.9, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYVTETLSNLIHQTAFFNLTWGNYLMIAVACVFLYLAIKKGFEP
                     LLLVPIAFGMLLVNIYPDIMANPEDMSNGVGGLLHYFYILDEWSILPSLIFMGVGAMTD
                     FGPLIANPISFLMGAAAQLGIYAAYFLAIFLGFNGKAAAAISIIGGADGPTSIFLAGKL
                     GQSALMGPIAVAAYSYMSLVPIIQPPIMKLCTTEKERKIKMDQLRPVSKLEKILFPIVI
                     TIVVCLILPTTAPLVGMLMLGNLFRECGVVKQLTETASNALMYIVVILLGTSVGASTSA
                     EAFLNADTLKIVVLGLVAFAIGTFGGCMLGKLLCKLTHGKINPLIGSAGVSAVPMAARV
                     SQKVGAEADPTNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     CDS             complement(3401..3769)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 62.8; E-value:
                     6.6e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 7.4e-21, bitscore:
                     62.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYTITVNGTAYEVTVEETGSVSAPAAAPKAAPKAAPAAAPKAA
                     VPAAGAGAVKVTASVPGKVVKVAASVGQAVKAGDSVIILESMKMEIPVVAPQDGTIASI
                     DVAEGASVENGDTLATMN"
     CDS             complement(3797..4552)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_6"
                     /sec_met_domain="OAD_gamma (E-value: 9.5e-28, bitscore:
                     85.7, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMIKKITSVFMTFLVMAALVIGSTSVVFAADKVDDTVKSTLVTT
                     AEGLTDAIVQLKDEEIENYMSSGDDFTTSAMQSWQTSKDELGAKKDSNGETTVTFKDDQ
                     YTVTVPLKFEKAEANFVYVFDSQGKPTSMTVDVQYGMGKTLQRAGLNTLMGIGTVFVML
                     ILLSLLISLFRFIPNPEAKKAAEAKAAKAAKEAEAAVIAQTAPAQAEENLADDGELVAV
                     IAAAIAAAEGTTTDGFVVRSIRKVKRNRR"
     CDS             complement(4567..6003)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_7"
                     /sec_met_domain="CT_Vparvula (E-value: 7.4e-137, bitscore:
                     445.7, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNATQSLAGTRITSLLDANSFVEIGQGVTARSTDFNMTANKAPS
                     DGVITGYGVIDDKLVYVYSQDASVLNGTVGEMHAKKITRLYDLAMKTGAPVIGLVDCAG
                     IRLQEATDALEAFGQIYLKQTLASGVIPQITAIFGTCGGGMAVIPSLTDFTFMESKKGK
                     MFVNTPNALEGNNTDKCDTAAADFQSKETGVIDGVGTEDEILGQIRSLVSLLPSNNEDT
                     DNYTECTDDLNRVCADLANCAGDTAIALSQIADNGEFFETKADYAKDMVTGFIRLNGAT
                     VGAVANRSEVYDAEGKKTETFDGSISARGARKAADFVKFCDAFDIPVLTLTNATGFMAT
                     LCSEKMMAKSVGELVAAFADATVPKVNVIIGKAYGTAYVAMNSKSIGADLVYAWDNAEI
                     GMMDASLAAKIMYADADAAELNEKAAQYRELQNGVASAAARGYVDTVISPADTRKYVIG
                     AFEMLFTKREDRPSKKHGTV"
ORIGIN
        1 gcttctgatt caagggtatt tacggaatct gttacagatt ccgttacagg tttctttaca
       61 atttcctgta cagcagtgtg aagatgaatc tttgctcctg ctttcttcag ttcacgttcc
      121 aatgcgcgga tgatgtctga ggaatggtca gactgtggaa atacgcggtt tccacgctct
      181 accttcagag ggacacctgc tttctcaaag aactccatta catcctgagg ggtaaatgaa
      241 tagaaactgc tgtagaggaa cttgggattg ctcatcattg caggaaagag ttcttctgtg
      301 tcgcaggcat ttgtcacatt gcatctgcct tttcctgtga tgaagacttt ctttcccagt
      361 ttctagttct tctctaatat atgaacttca tgatggttac gggctgcaaa aacccctgcc
      421 atcattccgg cggctccgcc gccgattatt ataactttgc tcatggaata ttctcctgta
      481 tttattttac cctgcagatt gtctatttca tcatggttcc acgtctccgc ttcgctacga
      541 tcaatataga tatgtacctc tggcatcata tacccattgg cacattatat tcccgggtat
      601 catattcttt cataataacg caaaggagcc tgcttacgca agctcctttt ttcgtttgga
      661 catatttaat tactgatcaa ttatacagga tatgctccgt tctttttgtc agctgctttc
      721 tcgtcaactt ttgttttctg agcctgacgg tatttgaaga agtccatagc tacctgtggg
      781 aacagtgcgt atgttaatac gtcttcatcc tgttccttgt actgagccat ttcgctctca
      841 agtttctcaa gctcgttgtc aagaaggtct gcaggacggc atgtgatcgg tttctcatcg
      901 ccgatgcact tcttctgtac ttccggatta aacggtttta cagttgcgcc gtattttcct
      961 aacaggatat cttttgtttc ttttgtaacc attttgtatc tctcgcccat aagtacgttg
     1021 aatacagcct gtgtaccaac gatctgggaa gacggagtaa caagcggcgg ttcaccaagg
     1081 tctttacgta cacgcggaac ttcctcgaga acatcgtaga atttgtcttc tgcgtgctgt
     1141 tctttcagct gggaagtcag gttggagagc ataccgcccg gtacctggta cagtaaagtt
     1201 ttgatgttaa caccaaggtt cttcggattg agcagtccgc tgtcaagagc ttcgtcacgg
     1261 atcggacgga agtagtcagc gatctcggag agaagcttct gatcaaatcc tgtgtcgtat
     1321 ggtgtatctt tgaatgtctc aaccataacc tctgttgcag gctgggatgt tcccagtgca
     1381 aatggagaca ttgctgtatc gatgatatcc acacctgctt ctactgcttt taagtaagtc
     1441 atggaagcta cacctgaggt gtaatgtgta tgaaggtcga tcgggatctt aactgtttct
     1501 tttaatgctg ttacaagctc tgtagctttg tttggaagga gaagacctgc catatctttg
     1561 atacagatgg aatcagcgcc catatcttcg atacgttttg caatatctgt ccagtattcc
     1621 attgtgtaag catctcctaa tgtataggaa agagctacct gtgcatgagc cttctctttg
     1681 tttgcagcgc ttacagctgt ctgaaggtta cgaaggtcat tgagacagtc aaaaatacgg
     1741 atgatgtcga ttccgtttgc agcagatttc tgtacgaagt attctacaac gtcatctgcg
     1801 tatggacggt atccaaggat gttctgtcca cggaaaagca tctgaagttt tgtgtttttg
     1861 aagccgtcac gtaatttacg aagtctttcc catggatctt cgtgaaggaa acgaagggat
     1921 gcatcgaatg ttgcaccacc ccagcactct actgcgtgat agccgacttt gtcaagttta
     1981 tctacgatcg gaagcatctg ttctgtggtc atacgtgttg cgatctggga ctgatgagca
     2041 tcacggagaa tggtttccac aattttaaca ggttttttct ctaattctgc cattattaaa
     2101 tctcctttat agttctgttt aatcggatat tcgcaatccg cttcgctact tgctcataac
     2161 caaatagctg ctccgcagct tatcagaagg agttgcactc ctcctgatac aagcaagata
     2221 gcttgcttga tttggttata ctccgaaaat agccatgaat gttccggctg ctacggctgt
     2281 accgataaca ccggcaacgt tcgggcccat tgcgtgcatc agaaggaagt ttgtaggatc
     2341 tgcctctgca cccactttct gggatacacg ggctgccata ggtactgcgg atacacctgc
     2401 ggaaccgatc agcgggttaa tctttccatg agtcagtttg cagagcagtt taccaagcat
     2461 acatccgccg aatgtaccga ttgcgaatgc aacaagacct aaaactacga tcttcagagt
     2521 atctgcgttt aagaatgctt ctgcacttgt ggaagcacct acggatgtac ccagaaggat
     2581 aactacgata tacatcaggg cattggaagc tgtttctgtc agctgtttta caacaccgca
     2641 ctctctgaac aggttaccaa gcatgagcat accaaccagc ggagctgttg taggcaggat
     2701 caggcagaca acgattgtga taacgatcgg gaacaggatc ttctccagtt tggaaaccgg
     2761 gcgaagctgg tccattttga tcttacgttc tttctctgtt gtgcagagtt tcatgatagg
     2821 cggctggatg atcggaacca gtgacatata ggaatatgct gccactgcga tcggtcccat
     2881 cagtgcagac tgtccaagtt tacctgcaag gaaaatggaa gtcgggccgt cagcaccacc
     2941 aatgatggag atagctgctg ctgcttttcc gttgaatcca agaaagattg caaggaagta
     3001 agctgcatag ataccaagct gtgctgcagc acccatcagg aagctgatcg ggtttgcgat
     3061 cagcggaccg aagtctgtca tggcacctac acccatgaag atcagggatg gtaagatact
     3121 ccactcatca aggatgtaga aataatgaag aagtccacct acaccattgg acatatcttc
     3181 cgggtttgcc ataatgtccg gatagatatt tacaagcagc ataccgaatg cgataggaac
     3241 aagcagaagc ggctcgaatc ctttcttgat agcaagataa aggaatacgc aggctactgc
     3301 gatcatcagg taattgcccc atgtcagatt gaaaaatgct gtctggtgaa tcaggttgga
     3361 caatgtttct gttacataag acatttgact acctcccgtc ttagttcatt gttgctaaag
     3421 tatctccgtt ttctacagag gcaccttctg caacatcaat gcttgcgata gtaccgtcct
     3481 gaggagctac tacagggatt tccattttca tggattcaag aataattacg ctgtctccgg
     3541 cttttactgc ctgtccaacg cttgctgcta ctttgacaac tttaccaggt acggaagcag
     3601 ttactttcac tgcgccggcg cctgctgcag gaactgctgc tttcggagct gctgcaggtg
     3661 cagccttagg tgctgctttt ggagctgctg caggagcgga tacgcttcct gtttcttcaa
     3721 cagttacttc atatgctgta ccatttactg taattgtata gcttttcatt ttaaaatcct
     3781 cctaaataaa tgacaattat cttctatttc tttttacttt acggatggaa cgaactacga
     3841 agccgtctgt tgtagttcct tctgctgctg cgatggctgc tgcgataaca gctaccagct
     3901 ctccgtcatc tgcaaggttc tcttctgcct gtgcaggtgc tgtctgtgcg atgactgctg
     3961 cctctgcctc ttttgcagct ttggctgctt ttgcttctgc tgctttcttt gcttccggat
     4021 tcggaatgaa tctgaacaga gagatcagaa ggcttaataa aatcagcatt acgaatacag
     4081 ttccaatacc catcagagta ttaagacctg ctctctgaag agtcttgccc ataccatact
     4141 gtacatctac agtcatggaa gtcggttttc cctgtgagtc aaagacatat acaaagtttg
     4201 cttctgcttt ctcaaattta agaggaacag taactgtgta ctgatcatct ttgaaagtta
     4261 cagtggtttc accattgctg tccttctttg cgccaagttc atccttggat gtctgccagg
     4321 actgcatggc acttgttgta aagtcatctc ctgaactcat gtaattctcg atttcttcat
     4381 ctttaagctg gacaatggca tctgtcagtc cttctgctgt agttaccagt gtggacttaa
     4441 cagtatcatc aaccttatct gcggcaaata ccacagaggt gctgccgatc accagcgcag
     4501 ccattacgag aaatgtcatg aatacagaag ttattttttt gatcattttc atatgcgtca
     4561 ccccgcttag accgttccat gtttcttaga cggacgatct tctctctttg tgaacagcat
     4621 ctcaaatgca ccgattacat attttcttgt gtcagccggg ctgatcacag tgtcaacata
     4681 gcctcttgct gctgcggaag caacaccatt ctgaagctct ctgtactgag cagctttttc
     4741 attcagttca gcagcatctg catctgcata catgatcttg gctgccaggg aagcatccat
     4801 cataccgatc tctgcgttat cccatgcata tacaaggtct gcaccgatag atttgctgtt
     4861 cattgccacg taagctgtac cgtaagcttt gccgatgatc acatttacct tcggaacagt
     4921 tgcatctgcg aatgcagcta ccagttcacc tacggatttc gccatcatct tctcgctgca
     4981 taaggttgcc atgaagcctg ttgcgtttgt aagtgtaagt accggaatat caaaagcgtc
     5041 gcagaatttc acgaaatctg ctgcttttct tgcgcctctt gcagagatgc ttccgtcaaa
     5101 tgtttctgtt ttcttgcctt ctgcgtcata tacttcgctt ctgtttgcaa cagcacctac
     5161 tgtagcgccg tttaaacgga taaagcctgt aaccatgtct tttgcatagt ctgcttttgt
     5221 ctcgaagaat tcaccgttgt cagcgatctg ggaaagtgcg attgctgtgt cacctgcaca
     5281 gtttgcaaga tctgcgcata cgcggttaag atcatctgta cactctgtgt aattgtctgt
     5341 gtcttcatta ttagaaggaa gaagtgatac taaggaacgg atctggccaa gaatctcatc
     5401 ctctgtacca acgccatcga tcacacctgt ctctttgctc tggaaatcag ctgctgctgt
     5461 gtcgcatttg tctgtgttgt ttccttccag tgcgtttggt gtgttgacga acatttttcc
     5521 ttttttggat tccatgaatg tgaaatctgt cagggacggg ataactgcca taccaccgcc
     5581 gcatgttccg aagattgctg tgatctgcgg gatcacaccg gatgccagtg tctgtttcag
     5641 atagatctgt ccgaaagctt ccagtgcgtc tgttgcttcc tgaagacgga tacctgcaca
     5701 gtcaacaagt ccgataaccg gagcgcctgt tttcatggca agatcataaa gtcttgtgat
     5761 cttctttgca tgcatttcac caacagtacc gtttaataca gatgcatcct ggctgtatac
     5821 ataaaccagt ttgtcatcga tcacaccata tccggtgata acaccgtcag atggtgcctt
     5881 gtttgcagtc atgttgaaat ctgtggatct ggctgttaca ccctgtccga tttcgacaaa
     5941 actgtttgcg tcaagcaaag aagtaattct tgtgcctgct aagctttgtg ttgcattact
     6001 cattcttgtt tgccctccat ctttatgaga atatttaaaa gttaacccaa aaatttagcc
     6061 gattttaatt ttaccttttc cggcagaaaa tttcaacatt tatttgtcat tttcgttaaa
     6121 aatatagcag aaaaatttgt caggtttctc caaattttgt ttttttgagc atttcttcct
     6181 atttaataga gggtattacg gttattttgt attgtcctgt atttcaccct ctcccgcaaa
     6241 ccagtgtgtc gacaagttga tttctcgaac aatcctgcgt ctgaatttct tatttctatc
     6301 ctgcaaga
//
