LOCUS       c00114_gnlPro..         5773 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NGKEMLHH_114.
ACCESSION   c00114_gnlPro..
VERSION     c00114_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 02:55:42
            Original ID  :: gnl|Prokka|NGKEMLHH_114
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5773
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5773
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2674:5689]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2675..5689
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5773
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5773
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             264..380
                     /locus_tag="ctg114_1"
                     /transl_table=11
                     /translation="MIGNSTLGPEHRELLVAEKQWKEIVELAFEQLPEIIQE"
     CDS             613..2268
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HMGL-like"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg114_2"
                     /sec_met_domain="HMGL-like (E-value: 8.7e-77, bitscore:
                     246.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNPSKYQRQYFMPPVKCMKWAEKEYVDKAPVWCSVDLRDGNQAL
                     VIPMSLEQKIEFFKLLVKIGFKEIEVGFPAASETEYEFLRTLIEQNLIPQDVTIQVLTQ
                     AREHIIRKTFEAVKGAPKAIVHVYNSTSVAQREQVFKKSKEEILKIAVDGAALLKKLAD
                     ETEGNFQFEYSPESFTGTEPEYALEVCNAVLDVWQPTADNKCIINLPVTVQHSMPHVYA
                     SQVEYMCENLKYRENVIVSLHPHNDRGCGVADSEMGLLAGADRIEGTLFGNGERTGNVD
                     IVTLGMNMYSQGVDPKLDFSDMPHICEIYEECTGMKVGERSPYSGALVFAAFSGSHQDA
                     IAKGMHWRDDKDPDHWNVPYLPIDPTDVGRNYDADVIRINSQSGKGGVGYILETKFGLN
                     LPPKMREAMGYATKAVSDHKHKELHPDEIFNLFKQTFENITEPYSINEVHFQQKDGGII
                     TKVTSTFRGKTITTEASGNGRLDAVSNALKKAYELKYSLETYQEHALERSSSSKAIAYV
                     GIKKPDGTLAWGAGVDADIIRASIDALVTAINNR"
     CDS             2675..4930
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg114_3"
                     /sec_met_domain="Gly_radical (E-value: 3.2e-42, bitscore:
                     131.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-268, bitscore:
                     880.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNFEQWNGFKGNRWKEKIDVRNFIGMNYTPYDGDASFLEGPTEA
                     TNKLWGKLQALQKEERAKGGVLDMETEVVTSLTAYGPGYIDEETKDLEKVVGLQTDKPL
                     KRAFMPYGGIKMAEQACETYGYKVSDKIKDVFHNYEFKTHNQGVFDIYTPEMKVARHNK
                     ILTGLPDTYGRGRIVGDYRRVALYGIDALIEGKQKDFAACDRQGMRRYDFQLREEIADQ
                     IRALKGMKVMAESYGYDISKPAKDAREAFQWLYFGYLAAIKTQNGAAMSVGRISTFLDI
                     YIERDLQNGTLTEKEAQELVDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVAGMGQD
                     GRSMVTKNDYRFLHTLEDMGPAPEPNLTVLYSSRLPENFKKYAANISVTTSSVQYENDD
                     VMRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYAINGGVDEKTKQQVGPNYQ
                     PITSEYLDYDEVIAKYDKMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTKVDRSF
                     ATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDFPRYGNDDDRADEIATTLL
                     STFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDGRKAGEPLAPGANPSYGAE
                     QNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDERSENLVHVLDGYFDQGAHH
                     LNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     CDS             4949..5689
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg114_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-28, bitscore:
                     87.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPDGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
ORIGIN
        1 ggtatcatga gagaggggca tctgccctct ctcaatctaa aaaatagttg caaaataaaa
       61 aacggattgt tataataaca tccatcgggg tatggcgtag cttggtagcg cgcacggctg
      121 ggggccgtga ggtcgcaggt tcaaatcctg ttgccccgac tgacatatgg ttaaatcgtt
      181 tgtagtatat ttttacaatg aatgattcag acgtatgttc atttcatttc aaaaaagcca
      241 aataaagtta taagtaaaat gcaatgatag ggaatagtac actcggaccg gagcacagag
      301 aactgctggt tgctgagaag cagtggaagg aaattgtgga actcgccttt gagcagctgc
      361 ctgaaattat acaagagtag gtgcagacgg tttccaccgt tatatggaga ggatataaga
      421 ataccggata tccggagatt ccgtatccca tgagtgtatg acattgttca taaataagag
      481 tggtaccgcg taagatgata atatctttcg tctcttgcag ataagtctgt ggagatggaa
      541 gattttttta tgtcttttta cagtcaatcc ccacagtcca aacataatat ttacataacg
      601 gaggaagaaa aaatgatgaa tccaagcaaa tatcagagac agtattttat gccgcctgtt
      661 aaatgcatga aatgggcaga aaaagaatat gtggacaaag cacctgtctg gtgttctgta
      721 gaccttcgtg acggtaatca ggcactggtg attccgatga gtctggaaca gaaaattgaa
      781 ttctttaaac ttcttgtaaa aatcggtttc aaagaaattg aagtaggttt cccggctgct
      841 tctgaaacag aatatgaatt tctgcgtacc ctgatcgagc agaatctgat cccacaggat
      901 gtaactattc aggttctgac tcaggccaga gagcatatca tccgcaaaac ttttgaagct
      961 gtcaaaggcg caccgaaggc aattgtacat gtgtacaatt ccacctctgt agctcagaga
     1021 gaacaggtat ttaagaaatc caaagaagaa atcttaaaga ttgcagtgga tggtgccgca
     1081 ttattaaaga aactggctga tgaaacagaa ggaaatttcc agtttgagta cagccctgaa
     1141 agctttacag gcacagaacc ggaatacgct ctggaagtat gcaatgcagt tcttgatgta
     1201 tggcagccta cagcagataa caaatgcatc atcaatctgc ctgtaactgt acagcattcc
     1261 atgccgcatg tatatgcaag ccaggtagaa tatatgtgtg aaaatctgaa ataccgcgag
     1321 aatgtaatcg tatctctcca tccgcacaat gacagaggat gcggtgttgc agattctgaa
     1381 atgggattac ttgcaggtgc agacagaatc gagggtactc tgttcggaaa cggtgagcgt
     1441 accggaaatg tagatattgt tacacttggt atgaatatgt attctcaggg tgtagatcca
     1501 aaacttgact tctctgatat gccgcatatc tgtgaaatat atgaagaatg taccggaatg
     1561 aaagtaggcg aaagaagtcc gtacagtggt gcacttgtat ttgccgcatt ttccggctct
     1621 catcaggatg caattgccaa gggtatgcac tggagagatg acaaggatcc ggatcactgg
     1681 aatgttccgt atcttccgat cgacccgaca gatgtgggaa gaaattatga cgcagatgtt
     1741 atccgcatca acagccagtc cggcaaaggc ggcgtaggat atattcttga gaccaaattc
     1801 gggcttaatc ttcctccgaa aatgcgtgaa gcaatgggat atgccacaaa agctgtttca
     1861 gaccacaaac ataaagagct tcatcctgac gaaattttca atctgtttaa acagaccttt
     1921 gagaatatca cagaaccgta cagcatcaat gaagtacatt tccagcagaa agatggtggt
     1981 attataacaa aagttacctc tactttccgc ggaaagacaa tcactacaga ggcatccggt
     2041 aacggacgtc ttgatgcagt cagcaatgca ctgaagaagg catatgagct gaaatattcc
     2101 ctggaaacat atcaggaaca tgcgctggag cgcagttcaa gttccaaagc tattgcttat
     2161 gtcggtatca aaaagccgga tggaaccctt gcctggggtg caggcgtaga cgcggatatc
     2221 attcgcgcat ctattgatgc acttgtaaca gcgatcaata accgttagat tgttccgaca
     2281 cgcccgattt tttcctgctg ttttttgact tctgcaggtc aggctaatat ccgcttggca
     2341 gggaataata tagtttacat aaaatatcaa aaatcagata ccggtcagat tgtccagagg
     2401 acttcctgac cggtattttt tttgtattta taaaaataca agaaagtctt aatgtacgcg
     2461 ataaagtaca ataaagaagc aaactccgat gaaaagctat cagaattgta tttaattaaa
     2521 atcataaggg aaattaattg acataacact catgtttacg tatactgaca gcgtggacag
     2581 aaaagtgtcc taaataagta caatttacat tttattatct cactccaggc cagtgagaaa
     2641 tgcgcggaaa tattttagag aaagaggtaa aagaatgaaa aatttcgaac agtggaatgg
     2701 ctttaagggc aaccgttgga aagaaaaaat cgatgtacgt aactttattg gaatgaacta
     2761 tacaccatat gacggtgatg cttctttcct ggaaggacct acagaagcta caaacaaact
     2821 gtggggcaag ctccaggcac ttcagaaaga agaacgtgct aaaggcggag ttcttgatat
     2881 ggagacagag gtagttacat ctctgacagc atatggtccc ggatatattg atgaagaaac
     2941 aaaagacctt gaaaaagttg ttggtcttca gacagataaa cctctgaaac gtgctttcat
     3001 gccatacggc ggtatcaaaa tggcagagca ggcttgtgaa acttacggat ataaagtaag
     3061 tgacaagatt aaagatgttt tccacaacta tgaatttaaa actcacaacc agggtgtatt
     3121 tgatatctac acacctgaga tgaaagtagc acgtcacaac aagatcctta caggtcttcc
     3181 ggatacttac ggacgtggac gtatcgttgg cgactacaga cgtgtggctc tgtacggaat
     3241 cgacgctctg atcgaaggaa aacagaaaga cttcgcagca tgcgaccgtc agggtatgag
     3301 acgttatgac ttccagcttc gtgaagagat cgctgatcag atccgtgctc tgaaaggcat
     3361 gaaagttatg gctgaatctt atggatatga tatctccaaa ccggccaaag atgcaagaga
     3421 agctttccag tggctgtact tcggatatct tgcagccatc aaaactcaga acggtgctgc
     3481 aatgagtgtc ggacgtattt ccacattcct tgatatttat attgaaagag atcttcagaa
     3541 cggaactctt acagagaaag aagctcagga gcttgttgac cacatggtaa tgaaattccg
     3601 tatggttaaa tttgcacgta tcccttctta caaccagtta ttctccggtg acccggtatg
     3661 ggcaactctg gaagtagcag gtatgggaca ggacggacgt tccatggtaa caaagaacga
     3721 ctatcgtttc ctgcacacac tggaagatat gggaccggct ccggaaccaa accttacagt
     3781 actttattct tcaagactgc ctgagaactt caagaaatat gctgcaaata tttctgttac
     3841 aacaagttct gttcagtatg agaatgatga tgttatgcgt ccggtatggg gcgatgacta
     3901 cagcatctgc tgctgtgtat ctgctacaga gacaggtaaa gagatgcagt tcttcggcgc
     3961 tcgtgcaaac cttgcaaaat gccttcttta tgcgatcaac ggcggtgttg atgagaagac
     4021 aaaacagcag gtaggaccaa actatcagcc gatcacttct gaatatcttg actatgatga
     4081 agtaattgca aaatatgata aaatgatgga ctggctggct catctgtatg tcggaacact
     4141 gaacatgatc cactacatgc atgacaaata ctactatgaa gcagcagaga tggctctgat
     4201 cgatacaaag gttgaccgtt ccttcgcaac tggtatcgca ggattctctc atgtagtaga
     4261 ctccctgtcc gctatcaaat atgcaaaagt taaagctatc cgcgatgaag acggtattac
     4321 aacagatttc caggttgagg gtgacttccc acgttatggt aatgatgatg acagagcaga
     4381 cgagattgca acaactcttc tgtctacatt ccttgagaaa ctgaaacaca tccatactta
     4441 ccgtgattcc aagccaacca catctatcct tactattact tccaacgttg tttatggtaa
     4501 ggctactgga tctcttccgg atggaagaaa agcaggggaa cctcttgcac ctggcgcaaa
     4561 cccatcttac ggtgcagagc agaacggtct tcttgcttcc ctgaactctg ttgccaagct
     4621 tgactatgaa gatgctcttg acggtatctc caatactcag acaatcaatc ctgatgctct
     4681 gggacacagt gatgacgagc gttcagagaa tctggtacat gtacttgacg gatactttga
     4741 tcagggtgct catcacctca atgtaaacgt atttggtaaa gagaaactga tcgacgctat
     4801 ggaacatccg gaaaaagaag agtatgcaaa cttcacaatc cgtgtatccg gatatgctgt
     4861 taagttcatc gaccttacaa gagagcagca gttagacgtt attgccagaa cctgccacga
     4921 cagaatgtaa ttaatggatg atgatataat ggataataaa cagataaaag gctatgttca
     4981 ttctttagaa agcttcgggt ccgttgacgg acccggagta agatatgtga tttttttaag
     5041 cggctgtgct atgagatgtc agttctgtca taatccggat acctggaaga tgaagcaggg
     5101 agagctttat acggcagatg agcttcttaa gaaagcttta cgttataagg gctactgggg
     5161 cagcaaaggc ggtatcactg taagcggtgg tgagccattg cttcagatgg attttcttac
     5221 agaatttttt aagaaggcaa aggcagaggg tgtgcatacg aatcttgata ccagtggaaa
     5281 tcccttcact gatcaggaac catggcattc cggatggctg gagcttatga agtatacaga
     5341 tcttgtaatg cttgatatca agcagattga cgagcaggag catattaagc ttaccggtca
     5401 ttccaataag aatattcttg cgatggcgcg ggaactttct gatatgaaaa aacctgtatg
     5461 gatccgtcat gtgcttgtgc cggacggaag tgataaggat gaatatctgc accggttagc
     5521 tgattttatc catactcttt ccaatgtgga gagagtggag gtgctccctt atcatacgct
     5581 tggtaagttt aaatgggaga atctggggct ttcttatcca ctggaggggg ttaatcctcc
     5641 gacgcaggaa aggattgata atgcccggaa gattcttgga gctatctgag taaaaatata
     5701 tagctgtcat atcctctttt tggggtatgg cagctttatt tgtttaaaaa agggacgcga
     5761 agcaggcgta tag
//
