LOCUS       c00377_gnlPro..         3138 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NGKEMLHH_377.
ACCESSION   c00377_gnlPro..
VERSION     c00377_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 02:55:42
            Original ID  :: gnl|Prokka|NGKEMLHH_377
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3138
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3138
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:3031](-)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      complement(2..3031)
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3138
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3138
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(2..3031)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg377_1"
                     /sec_met_domain="HGD-D (E-value: 5.1e-25, bitscore: 74.9,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 9.9e-39, bitscore:
                     119.5, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYKTLGIDIGSTTVKIAILDENENLIFADYKRHFANIQETLADL
                     LQEAFDQYGEMTLHPVITGSGGLTLANHLGVPFTQEVIAVSTSLQKLAPKTDVAIELGG
                     EDAKIIYFENGNVEQRMNGICAGGTGSFIDQMASLLQTDATGLNEYAKDYKALYPIAAR
                     CGVFAKTDIQPLINDGATKPDLSASIFQAVVNQTISGLACGKPIRGHVAFLGGPLHFLS
                     ELRESFIRTLKLDGEHTIIPENSHLFAAIGSALNAKEDNSISLTEMKDRLRNSIKLDFE
                     VERMEPLFASQEEYDAFAKRQSSYKVKTGDLATYKGNCYLGIDAGSTTTKTALVGEDGT
                     LLYSFYSSNNGNPLKTTIRSIKEIYELLPKDAKIAYSCSTGYGEALIKAALILDEGEVE
                     TVSHYYAAAFFDPEVDCILDIGGQDMKCIKIRNKTVDSVQLNEACSSGCGSFIETFAKS
                     LNYTVQDFAKAAVFAQHPIDLGTRCTVFMNSKVKQAQKEGAEVSDISAGLAYSVIKNAL
                     YKVIKVSDASELGRHIVVQGGTFYNDAVLRSFEKIAGCEAIRPDIAGIMGAFGAALIAR
                     ERHQEGAETTMLSIDKINELKYTTSMANCRGCTNNCRLTINKFSGGRQYVSGNRCERGI
                     GKEKNKDHIPNLYEYKYKRIFSYAPLTADKATRGKVGIPRVLNMFENYPFWYTFFTELK
                     YEVVLSPTSTRKIYELGIESIPSESECYPAKLAHGHVTWLIRNGVKFIFYPCIPYERNE
                     FPDAVNHYNCPIVTSYAENIKNNVDELNDPSITFRNPFLAFTSEEILANRLVEEFKDIP
                     AEEVKAAVHKGWEEMAAARRDVQKKGEETLKYLEDTGRHGIVLAGRPYHIDPEIHHGIP
                     DLINSYGIAVLTEDSISHLAPVERPIRVNDQWMYHSRLYAAANYVKTRDDLDLIQLNSF
                     GCGLDAVTTDEVYEILDGSDKIYTCLKIDEVNNLGAARIRIRSLIAAIRAKKAQGQKRT
                     VKPASIDKVSFTKEMRKDYTI"
ORIGIN
        1 ggatggtata gtcctttctc atttcctttg taaaggatac tttatcaata gaagcaggtt
       61 tgacagtacg tttctgtccc tgtgctttct tcgcgcggat cgctgcgatc agggaacgga
      121 tacggattct tgcagcacca aggttattta cctcgtcaat ctttaagcag gtatagattt
      181 tgtcacttcc gtcaaggatt tcataaacct cgtcagtagt aactgcatcc agaccacatc
      241 caaaagagtt gagctggatc agatccaggt catcgcgtgt ctttacatag tttgcagcag
      301 catacagtct ggagtggtac atccactggt cattaacgcg gatcgggcgt tccacaggtg
      361 ccagatgaga gatggaatcc tcggtaagta cggcaatacc atagctgttg atcagatccg
      421 ggataccatg atggatttcc gggtcaatat ggtatgggcg tcctgcaagg acaataccat
      481 ggcgtcctgt atcttccaga tatttcaggg tttcttcccc ttttttctgt acatcgcggc
      541 gtgcagcagc catttcctcc cagcccttat gcacagcagc tttgacttct tctgccggga
      601 tatccttaaa ttcctctacc agacgatttg ccagaatttc ctcagaagta aatgcaagga
      661 acggattgcg gaaggtaatg gacggatcat ttaattcgtc tacgttattt ttgatatttt
      721 cggcataaga ggtaacgatc gggcagttat agtgattgac tgcatccggg aactcgttac
      781 gttcatatgg gatacatggg tagaaaataa atttcacgcc attgcggatc agccaggtta
      841 catggccatg ggcaagtttg gcaggatagc attcagattc acttggaatg gactcaatac
      901 ccagctcgta aatctttctg gtggaagtag gagagagcac aacctcatac ttcagttctg
      961 taaagaaagt ataccagaaa ggataattct caaacatgtt cagaacacgg gggataccta
     1021 ctttacctcg cgttgcctta tcagcagtaa gcggtgcgta ggagaaaata cgcttatatt
     1081 tatattcata aagattcgga atatgatctt tgttcttttc cttaccaata ccgcgttcac
     1141 agcggttacc gcttacatac tggcggccgc cggagaattt gttgatagtc agacggcagt
     1201 tattggtaca tccacgacag tttgccatag aagttgtata tttcagctcg ttgatcttgt
     1261 caatggagag catagtcgtt tctgcgcctt cctgatggcg ttcacgggca atcagtgcgg
     1321 caccgaaggc acccatgatt ccggcaatat ccggacggat ggcttcgcag cctgcaatct
     1381 tttcgaagct tcgaagaacc gcatcattgt agaaggtacc accctgtaca acgatgtgtc
     1441 tgccaagttc ggaagcatca gaaaccttaa taaccttata gagtgcattt ttgataacag
     1501 agtaagcaag acctgcggaa atatcggata cctcagctcc ttctttctgt gcctgcttta
     1561 ccttggaatt catgaaaaca gtacatctgg taccaagatc tataggatgc tgtgcaaaaa
     1621 cagcagcttt tgcgaaatcc tgtactgtat aattcaggga tttggcaaag gtttcaataa
     1681 aggaaccgca tccggaggag catgcttcat ttaactgtac gctgtcaacg gtcttgttgc
     1741 ggatcttgat acatttcata tcctgtccgc cgatatccag gatacagtct acctccgggt
     1801 caaaaaacgc tgctgcatag taatgggata cggtctcaac ctcgccctca tccaggatca
     1861 gagcagcctt aatcagagcc tcgccgtaac ctgtagaaca ggaataagcg atctttgcgt
     1921 ctttgggaag cagttcatag atttctttaa tagaacggat ggtagttttt aacgggtttc
     1981 cgttgttgct gctgtaaaag gaatagagaa gtgttccatc ttcgcctacc agtgcagttt
     2041 tggttgtggt ggaacctgca tcaataccca gatagcagtt tcctttatat gtagccagat
     2101 ctcctgtttt tactttgtag gaactctgac gttttgcaaa tgcatcgtat tcttcctgag
     2161 aagcgaaaag tggttccata cgttctactt caaaatccag cttaatggaa ttgcggagtc
     2221 tgtctttcat ttctgtcagg gaaatggagt tgtcttcttt ggcatttaat gcagaaccga
     2281 tggctgcaaa aagatgggag ttctccggaa tgatggtgtg ttccccatcc agcttcaaag
     2341 tacggataaa ggattctctc agttcggaca ggaagtgaag cggaccaccc aggaatgcaa
     2401 catgtccacg gatgggtttt ccacaggcaa gaccggagat ggtctggttt acaactgcct
     2461 ggaaaataga agctgacaga tctggtttgg ttgcaccgtc attgatcagc ggctggatat
     2521 ccgttttggc gaaaacaccg cagcgtgcag caatcgggta gagtgctttg taatctttgg
     2581 catattcatt cagacctgtg gcatctgtct ggagaagaga agccatctgg tcgatgaaag
     2641 agccggtacc gcctgcacag ataccgttca tacgctgttc tacgtttccg ttttcgaaat
     2701 aaatgatctt ggcatcctca ccgccaagct cgatagcaac atctgtcttt ggtgccagct
     2761 tctgcagaga agtagaaact gcaataacct cctgggtaaa aggaacgccc agatggttgg
     2821 caagggtcag accgccggaa cctgtgatca caggatggag agtcatctcg ccatactggt
     2881 caaaggcctc ctgaagaagg tctgcaaggg tttcctgaat gttcgcaaaa tgacgcttgt
     2941 aatccgcgaa aatgagattt tcattctcat ctaagatcgc gatctttaca gtagtggaac
     3001 caatgtcaat tccaagtgtt ttgtaattca taaagtaaat tcaagcctcc tcgtattgta
     3061 tataaacaaa attgaaattt tcctgttact gttcacacac cactatcccg cgaggtgttt
     3121 tggcatgaat atgccaaa
//
