LOCUS       c00440_gnlPro..         2812 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NGKEMLHH_440.
ACCESSION   c00440_gnlPro..
VERSION     c00440_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 02:55:42
            Original ID  :: gnl|Prokka|NGKEMLHH_440
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2812
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2812
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[38:2811]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      39..2811
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..2812
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2812
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(39..1718)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg440_1"
                     /sec_met_domain="HGD-D (E-value: 2.7e-18, bitscore: 53.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.9e-66, bitscore:
                     211.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKLDFLYILDVLHCDSACSRER
                     MAVQLKGLAKAYAQYKGTEFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTGRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGERKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLNPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLEFKED
                     ITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTRRVGGEEKYMMTGGV
                     SKNKGLVKTLEEKLGTKLVISDKAQLCGALGAALFAADMADV"
     CDS             complement(1711..2811)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg440_2"
                     /sec_met_domain="HGD-D (E-value: 2e-57, bitscore: 182.4,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DTMLWPLQKPEDSVIVSIFTPCEMMQEVGLHPYNVEGFSCYLSAS
                     KAERAFLQQAENQGIAETLCSYHKTFLGAAQKGLLPKPKCIVYTNLTCDANLLTFRTLA
                     DFYQVPVFAIDVPWNQTTENVQYVADQLKDLKIFLEKNTGKTISEDRLKERLACSKRTL
                     ENYKKYQQMRADRYVPSDLVTPLYAGMTNNILLGTAEEEKYTQMLLEDIKKAPAAKGKH
                     IYWMHTIPFWSDAVRQELCFSEKAQIVGCELAETCEPDFDPENPFEAMAERMVYHSLNG
                     SAFRRIEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCD
                     RSHGGEGQTSTRLGAFLEMLGGAENE"
ORIGIN
        1 tttccacctt gtaaaatccc tggatattta aataattttt atacatcagc catatcagcc
       61 gcaaacagtg ctgcgccaag tgcaccgcaa agctgagcct tgtcactgat caccagcttt
      121 gttccaagct tctcttccag tgtctttacc aggcccttat tcttagaaac accaccggtc
      181 atcatatatt tctcctcacc gcccacacgt ctggtaaggg cggcagtctt tacggcaacc
      241 gccttattca ggccatggac aatatcatct gttgccttat tctgggcgat cagggataca
      301 acttctgatt ctgcaaatac tgtacacata ctggaaattg taatatcttc tttgaattcc
      361 agcccacatt cgctcatctg atccagattc atttccatgg tacgtgccat catttccaga
      421 aatcgtccgg ttcctgccgc acatttgtca ttcataacaa agtttgcaac tgcaccgttc
      481 tcatccagtc ggatcacctt gctgtcctgt ccgccaatat caataacagt acggacttcc
      541 ggattcagga aatgcgcacc ccttgcatga caggtgatct ctgtgatact cttatcacca
      601 tttttaatag ctgtacgtcc gtatccagta gtaaccagcg catcaatatc ttctctctgc
      661 aatccggcct ccttcaatgc ttctgcaagg gcacggtctg ctccgatagc agctcctgct
      721 cctgtaggca ggataatgct ggtcacaatc tcatgatctt tatttaaaat caccacatct
      781 gtactggtgg aaccactgtc aatccctgcg aaataacctt ttcccatttt tctctctcct
      841 tttgtgtctc catctatggt ctgctccagt gtttcaggct gaatgctctc tgcaaaagct
      901 tccagtctgg taagaagctg accgctgctc tgaatagtat aatctgattc aatttttaaa
      961 agaggtacat ctgtatggtt cttgatctca gcatattcga agctgtaaaa atcacaaaac
     1021 ttcactgtat gataaataat ccccgcaaca gacggatcat tataaagctt cttgcgacct
     1081 gtcgggtcca tcatacgcat acatggaatc tgccccagaa tctcacctgc ataccagtcc
     1141 atcagctcat caaaagaagc accctccggt ggaagtatat ttcccacaga acggttatgc
     1201 acgcaggtat cattctctac aggaagaggc atagctttgc tggtcatttc aaataattcc
     1261 tgccccattc tggcgccaag cactgcaata tgggactttg tgattttctc ctgtgcatgg
     1321 aaagcagccc ggaattttcc ggcatcaaac tctgtgcctt tgtactgtgc ataagccttc
     1381 gccagccctt ttaactgtac agccatacgc tctctgctgc aggcactgtc acagtgaagc
     1441 acatccaaaa tatagagaaa atccagcttg ccgctttcaa gcagaatgtc atagacactc
     1501 cggatagtat cacagcagct caccaggaca agttccttga ttttcccctc catgactcct
     1561 tccagaagtg tttttccaaa accgcagata ttgggatgtg aaacctgatc ggccaggtca
     1621 aatccctccg gcatatgatt aaagttctca cattccccgc caagagattc cagaagttca
     1681 attggggtat atttacagac atagtaagtt ttattcattc tcagcacctc ccagcatttc
     1741 cagaaaagct cccagcctgg tggaagtctg tccttctcct ccatgacttc gatcacaccc
     1801 gtcaccgtcc aggatcaaaa ggggcagacc ggcttcctcg aacttcttct ttgcaagctg
     1861 tgccgcacca agcgtatgtt tacatcccca atgtccaaac cacacagcac cgtctgctcc
     1921 tgcctgtttc gcatggcgga taccggcttc aatccttcta aaggcacttc cgttcagtga
     1981 atgatatacc attctttctg ccattgcttc aaaagggttc tcaggatcaa aatccggctc
     2041 acaggtttct gcaagttcac atcctacgat ctgggctttc tcactgaaac aaagctcctg
     2101 gcgcactgca tccgaccaga atggaatggt atgcatccag taaatgtgct tccctttcgc
     2161 tgccggtgct ttcttaatat cttcaagaag catctgtgta tacttctctt cttctgctgt
     2221 tcccagaagg atattgtttg tcattcctgc atacaagggt gtgaccagat cagagggcac
     2281 atatctgtct gcacgcatct gctgatattt tttgtaattc tccagagtcc gcttactgca
     2341 ggcaagacgt tcctttaatc tgtcctcaga gattgttttt cctgtatttt tttccagaaa
     2401 tatttttaaa tcctttaact gatctgccac atactgtaca ttctctgtgg tctgattcca
     2461 tggtacatca atggcaaata caggtacctg gtaaaaatct gccagggtac ggaaggtgag
     2521 caggttagca tcgcaggtca ggttggtata aacaatacat ttaggctttg gcagaagtcc
     2581 tttctgcgct gcaccaagaa atgtcttgtg ataactgcac agtgtctctg caattccctg
     2641 attctctgcc tgctgcaaaa atgccctctc tgctttgctt gcagaaagat agcaggaaaa
     2701 tccttctaca ttatagggat gtaatcccac ctcctgcatc atttcgcagg gtgtaaaaat
     2761 acttacaata acagaatctt ccggtttctg aaggggccac agcatggtat cc
//
