LOCUS       c00009_gnlPro..         5435 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PLEAMBEB_9.
ACCESSION   c00009_gnlPro..
VERSION     c00009_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 04:46:17
            Original ID  :: gnl|Prokka|PLEAMBEB_9
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5435
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5435
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1804:5294]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1805..5294
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5435
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5435
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             254..517
                     /locus_tag="ctg9_1"
                     /transl_table=11
                     /translation="MNLQKQQLLLRLCRYFKSRKGEPRFFNKRNICLLAAASAYILTPD
                     EFLFDGIGKLGLLDDAAVILGAVLTVLFSGWRLTARTHRRRN"
     CDS             complement(514..1299)
                     /locus_tag="ctg9_2"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSVDGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFAREVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(1332..1604)
                     /locus_tag="ctg9_3"
                     /transl_table=11
                     /translation="VKRSKKEVLDADFAAARAGVLDVAAFLDRVDRGEGEADFRYRALM
                     EVLPLLSASGPGRVRAILEALSYRDDAVPETAAAKAACGAPNLSK"
     CDS             1805..2506
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg9_4"
                     /sec_met_domain="SucD_FumR (E-value: 6.3e-14, bitscore:
                     40.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLGVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLVYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             2528..4486
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 174; E-value:
                     1.9e-53)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg9_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 2.2e-54, bitscore:
                     174.0, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.3e-38,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYPALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAITGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTASPELKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             4527..5294
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg9_6"
                     /sec_met_domain="Fer2_3 (E-value: 2.6e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.4e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 4.8e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENRDAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
     CDS             complement(5361..5435)
                     /locus_tag="ctg9_7"
                     /transl_table=11
                     /translation="LFLATVKFPGRGYLVFYSWLKETR"
ORIGIN
        1 ggagggtcat ttcaagagct tccggccccg ccccacttcc ttacacgccc ttttttccca
       61 tgccggaaca gccagagccg gcccctgcaa agtccggatt ccctctgcat ccggaacagg
      121 ccatcctctc agcaattcat ttccccgcat gatccgagtc accgcgcttc cgtccgctgg
      181 aagcgacctc tcgccctgaa acagggggaa agaggggaaa tgactgagct ttaaccttca
      241 ttccccgttc ctcatgaacc ttcaaaaaca gcagctgttg ctgcgtctgt gccggtattt
      301 caaaagcaga aaaggagaac cgcgtttttt caacaaacgg aatatatgcc ttctggccgc
      361 cgccagcgcc tacatcctga ctcccgacga attcctgttt gacggaatcg gcaagctggg
      421 ccttctagat gacgcggcgg tcatcctggg agccgttctc accgttcttt tttccggatg
      481 gagactaacg gcccgcaccc accgcaggcg gaattagcgg gacgacactt tccggaactt
      541 cggagactgt cccatgaatg tctccggatt ttccaagaga agacgttccg tttcctgccg
      601 ggaaaaaccg cgcctgcgta gctcgcaggc cagagccgtc agcgccaggg gatcggaatc
      661 cccccagtct gccgcggaat tggcccagac acggtccatt ccgtacaatt ccagaatatc
      721 cgccgcccgc ttgggattca gcttgctggt gggataaagg gtcagtccgg cccagtagcc
      781 ggcctccttc accagcggga aggtatgttc ctcacagtga tccaccaaca cgcggcaagg
      841 atccagatcc ggacggtttt tcagggcgga aagaatcatt acggtcccct tcagcttgtc
      901 ttccaaatgg ggagtgtgaa tcagcacaag ctggttgagg cgcaccgcca gatccagatg
      961 ctcttcaaaa atggtcattt catgggaagt attccggttc agccctattt ccccgatgcc
     1021 aaggacattg ggccggtcaa taaactccgg aatgagtttc atcacctccc gtgcaaaggc
     1081 cacgtcttgc gcctctttgg gattgatgca caaccatgtg taatggtcca tgccgaactt
     1141 ggccgccctg gcgggttcgc attccgtaag ctgtacgaaa taatcccgga aaccgtccac
     1201 ggacgaacgg tcataaccgg cccagaacgc cggctcccct accgccaggc agcctgcctg
     1261 cgccatggcc cggtattcgt ccgtggtgcg gcacaccata tgtatatgag gttctatgaa
     1321 agccatcatt cttatttgct gaggttcgga gctccgcatg ccgctttggc cgcggcggtt
     1381 tccggcacgg cgtcatccct gtaggaaagg gcttccagaa tggcccggac gcgtccgggg
     1441 ccggatgcgg acaaaagggg aagaacctcc atcagggcac ggtagcggaa atccgcctct
     1501 ccttccccgc ggtccacccg gtccagaaat gcggccacat ccagaacgcc ggcgcgcgcc
     1561 gctgcgaaat ccgcgtccag cacctctttt ttactccttt tcacgcacag acgatacgcc
     1621 tgtgcggaaa gcctcgtcaa gccggaacag gtaacaaaat aataactatt ccaaactgat
     1681 aaacagcaac gcctttcccc ggcttttcaa aaatccgaaa agcgccggaa attcaagaag
     1741 aggcttgcca tccgggcttg aaccgggcat cctgtgagga cgcatttatt tcattagagc
     1801 gcctatgaat gcactagtaa aaaccatatg tacattcgtg acatcctcca tcggccgcaa
     1861 aatcattgtg gctctgacgg ggttatgttt ggtcttgttc cttgcgggac acctggcagg
     1921 caaccttctc atcttcggag gccctgaatg gatcaacacc tacgcccacg gcctgcactc
     1981 catgccggaa gcagccctct ggggcattcg cgcggggctt ggggtgatct tcatcatcca
     2041 tgtctggctg accatccagc tgaagctgga aaaccatgcg gcccgcgaac cctatgtgtt
     2101 caagaacacc atcaaggcga cgctctcctc ccgctacatg atttacaccg gcctgaccgt
     2161 cctggtgttc ctggtctacc acctgtacca atacaccctg cgcgtgggat acgaccccgc
     2221 ccagttcacc accttcattt ccgacggtac ggtggagacc tttgacgtgt acaaaatgat
     2281 cgtgaccgga ttctccaacg tatggtgctc cgccttctac atcctggccg ttctgatgct
     2341 gttcagccat ctgcgccacg gggtacagtc catcttccag actgtgggcg ttgattcccg
     2401 caaaatccgg cccttctaca attttatcgc catcgcctac ggcgcgatta tctgcctggg
     2461 cttcatctcc gtgccggtct cggttcttct gggcatcatc aaataaccat caactcctct
     2521 caaagccatg attaattttc ccgtaagcga ctggatgccg gatgccaaaa ttccctccgg
     2581 ccctattcag gacaaatggt ccaagtacaa gcttgaatcc aaactgatca atccgaacaa
     2641 caagcgcaag tacacggtgc ttgtggtagg ttccggcctg gctggcgcct ccgctgccgc
     2701 cacgcttgcc gaacttggct acaacgtcaa atgcttctgc tatcaggaca gcccccgccg
     2761 cgcccactcc attgcggccc agggcggcat caacgcggcc aagaactacc agaacgacgg
     2821 tgactccgtt taccgcctgt tttacgacac cgtcaagggc ggcgacttcc gcgcccgtga
     2881 agccaacgtt taccgcctgg cggaagtctc ctccaacatc attgaccagt gcgtggccca
     2941 gggcgttccc ttcggccgtg aatacggcgg cctcctggat aaccgctcct tcggcggcgc
     3001 ccagcttaag cgtaccttct acgcacgcgg acagacgggc cagcagctcc tgctgggtgc
     3061 ctacccggct ttggaaaagg aagtggccaa gggacacatt gaaatgttct cccgccatga
     3121 aatgatggac ctggtgctgg tggacggaga agccaagggc atcgtcaccc gcaatctggt
     3181 caccggtgaa atcaagtcct acgcagggga taccgtcctg ctctgcacgg gcggctacgg
     3241 caacgtgttc ttcctgtcca ccaacgcgat gggctgcaac gtcaccgccg cctacaaggc
     3301 ctacaagcgc ggcgctttct tcgccaaccc ctgcttcacg caaattcacc ccacctgcat
     3361 tccggtcaag ggcgactacc agtccaagct gacgctgatg tcggaatccc tccgcaacag
     3421 cggccgcatc tgggtgccca agtcccgtga aaccgcggaa aaaatccgca aaaaggaaat
     3481 caagccttcc gatgttcccg aagaagaacg ggactactac ctggaacgca aatatccctc
     3541 cttcggcaac ctggctccgc gcgacatttc ctcccgcgcc gccaaggaag cctgcgacga
     3601 cggccgcggc gtggccatca ccggcaaggg agtcttcctg gactttgcgg atgaaatcaa
     3661 gcgcatgggc cgcgaatgga ttgacggcca gtacggcaac ctgtttgaca tgtacgaaga
     3721 aatcacggac gacgaccccc atgaaacgcc catgatgatc taccccgcct cccactacac
     3781 catgggcggc ctgtgggtgg attacaacct catgtccaac gtgaaggggc tgcacgtcct
     3841 cggtgaagcc aatttctccg accacggcgc caaccgcctg ggagcctccg ccctgatgca
     3901 gggcctgtct gacgggtact tcgtcatccc agccacgctt cccgcctacc tgacgaccac
     3961 caagccgggc gccgtcacca ccgcctcccc ggaattaaag gccgcggaag acgaggtgaa
     4021 ggaacgcatc aacaaactcc tgaacgtgaa aggcaccaag acggtggact ccttccaccg
     4081 ggaactgggc ctcatcatgt gggaagactg cggcatggcc cgcacggaag aaagcctgac
     4141 ccacgccatt gaacgcattc cccagctccg caaggaattc tgggaaaacg tgcgcgtaac
     4201 cggcacggcg gacggcatca acgcggaact ggaaaaagcc tgccgcgtgg ctgacttcct
     4261 agaattcgcc cagctgctct gctatgacgc cctgaaccgt caggaatcct gcggcgcgca
     4321 cttccgcgtg gaataccagc tgccggacgg cgaagccaag cgcaacgacg atgaatacgc
     4381 ctacgtagcc gcctgggaat acaccggcgt ggacagcctg cctgttctcc acaaggaacc
     4441 gctgaccttc gagaacgtgc accttgccat ccgcagctac aagtaaagat agccaataca
     4501 ttaacactta aaagaaagat tataacatgg ctaaaacact caatctcact ctcaaggtct
     4561 ggcgccagga aaaccgtgac gccaagggcc gcattgagac ctatgccgcc aaggacattc
     4621 cggtggatgc ctccttcctg gaaatgctgg acatcgtcaa tgaaggtctg gtcaaggaag
     4681 gcaaggaacc catccatttt gaccacgact gccgggaagg catctgcggc atgtgctccc
     4741 tgaccatcaa cggcattccc cacgggccgg aacgccaggt aaccacctgc cagcttcaca
     4801 tgcgcaagtt caaggacggc gacaccgtct ggatcgaacc cttccgcgcg aaggctttcc
     4861 cgatcctgcg ggacctgatg gtggaccgtt ccgccctgga ccgcatcatc gccgccggcg
     4921 gctacattga cgtgcgcacc ggctccgcgc cgaacgccaa caacatcgcc gtggaaaaag
     4981 tgaaggcgga cgccgccttt gacgccgccg cctgcatcgg ctgcggcgcc tgcgtggcct
     5041 cctgcaaaaa cgcctccgcc atgctgttca cgtccgccaa aatcgcccat ctcaatctgc
     5101 tgccgcaggg ccagccggaa cgctccaagc gcgtcctggc catgatgaag cagatggaag
     5161 cggaaggctt cggcaactgc acgaaccaat acgaatgcga agccgcctgc ccgaaaggca
     5221 tcagcgtgga caacatcgcc cgcctcaacc gcgacttcat gcgggccaat gccaaggaag
     5281 gcctctgctt ctaaagagga ctccttctta agacgctcta caaaaactcc cggcggcctc
     5341 ggctgtcggg agttttttta ttaccgggtt tccttgagcc aggaataaaa taccagatag
     5401 cctcttccgg gaaatttgac agtggccagg aagag
//
