GCA_015549355.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaNCELMIHK_4 (original name was: gnl|Prokka|NCELMIHK_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	40,623	89,607	dipeptide aldehydes	NRP	52%

gnlProkkaNCELMIHK_9 (original name was: gnl|Prokka|NCELMIHK_9)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	cyclic-lactone-autoinducer	13,944	34,675

gnlProkkaNCELMIHK_11 (original name was: gnl|Prokka|NCELMIHK_11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	RRE-containing	137,591	157,857

gnlProkkaNCELMIHK_12 (original name was: gnl|Prokka|NCELMIHK_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	ranthipeptide	28,859	50,447

gnlProkkaNCELMIHK_83 (original name was: gnl|Prokka|NCELMIHK_83)

Region	Type	From	To	Most similar known cluster		Similarity
Region 83.1	NRPS	42,067	83,566

gnlProkkaNCELMIHK_84 (original name was: gnl|Prokka|NCELMIHK_84)

Region	Type	From	To	Most similar known cluster		Similarity
Region 84.1	lanthipeptide-class-ii	94,819	117,512

Compact view

gnlProkkaNCELMIHK_4 - Region 1 - NRPS

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Location: 40,623 - 89,607 nt. (total: 48,985 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 40622 - 89607:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg4_66

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaNCELMIHK_9 - Region 1 - cyclic-lactone-autoinducer

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Location: 13,944 - 34,675 nt. (total: 20,732 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNCELMIHK_11 - Region 1 - RRE-containing

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Location: 137,591 - 157,857 nt. (total: 20,267 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNCELMIHK_12 - Region 1 - ranthipeptide

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Location: 28,859 - 50,447 nt. (total: 21,589 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNCELMIHK_83 - Region 1 - NRPS

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Location: 42,067 - 83,566 nt. (total: 41,500 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNCELMIHK_84 - Region 1 - lanthipeptide-class-ii

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Location: 94,819 - 117,512 nt. (total: 22,694 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg84_105

ctg84_106

Leader:

MNEMNDKIIGNVEKELDEIEIIDTESIEEKLSFIVYG

Core:

TIVCC

Core with dehydrations:

DhbIVCysCys

allorf_115889_116002

Leader:

MICSLMVMNIRKALSAG

Core:

KIHPTERASVFCPYFLVSGL

Core with dehydrations:

KIHPDhbERADhaVFCysPYFLVDhaGL

antiSMASH-DB 4.0 matches for allorf_115889_116002

100.0% NZ_JADMYT010000009.1and 3 others:

NZ_JADNMI010000013.1 Lanthipeptides: allorf_010454_010567_lanthipeptideNZ_QUEF01000006.1 Lanthipeptides: allorf_116827_116940_lanthipeptideNZ_QUIT01000007.1 Lanthipeptides: allorf_004751_004864_lanthipeptide

Lanthipeptides: allorf_115889_116002_lanthipeptide

1

KIHPTERASVFCPYFLVSGL

20

KIHPTERASVFCPYFLVSGL

1

KIHPTERASVFCPYFLVSGL

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg84_106 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 519.2 Da

Molecular weight: 519.7 Da

Number of bridges: 1

allorf_115889_116002 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 2209.2 Da

Molecular weight: 2210.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2228.6 Da N = 1; 2246.6 Da N = 2