GCA_015554785.1 - 8 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaICLENCNL_3 (original name was: gnl|Prokka|ICLENCNL_3)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	phosphonate	1	16,378

gnlProkkaICLENCNL_4 (original name was: gnl|Prokka|ICLENCNL_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	cyclic-lactone-autoinducer	72,718	93,441

gnlProkkaICLENCNL_5 (original name was: gnl|Prokka|ICLENCNL_5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	ranthipeptide	86,180	108,028

gnlProkkaICLENCNL_6 (original name was: gnl|Prokka|ICLENCNL_6)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	cyclic-lactone-autoinducer	54,728	75,474

gnlProkkaICLENCNL_10 (original name was: gnl|Prokka|ICLENCNL_10)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	cyclic-lactone-autoinducer	12,500	33,248

gnlProkkaICLENCNL_11 (original name was: gnl|Prokka|ICLENCNL_11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	RRE-containing	211,836	232,087

gnlProkkaICLENCNL_19 (original name was: gnl|Prokka|ICLENCNL_19)

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	NRPS,cyclic-lactone-autoinducer	4,033	57,510	equibactin	NRP	21%

gnlProkkaICLENCNL_32 (original name was: gnl|Prokka|ICLENCNL_32)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	cyclic-lactone-autoinducer	1	20,333

Region	Type	From	To	Most similar known cluster	Similarity
Region 3.1	phosphonate	1	16,378
Region 4.1	cyclic-lactone-autoinducer	72,718	93,441
Region 5.1	ranthipeptide	86,180	108,028
Region 6.1	cyclic-lactone-autoinducer	54,728	75,474
Region 10.1	cyclic-lactone-autoinducer	12,500	33,248
Region 11.1	RRE-containing	211,836	232,087
Region 19.1	NRPS,cyclic-lactone-autoinducer	4,033	57,510	equibactin	NRP	21%
Region 32.1	cyclic-lactone-autoinducer	1	20,333

Compact view

gnlProkkaICLENCNL_3 - Region 1 - phosphonate

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Location: 1 - 16,378 nt. (total: 16,378 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaICLENCNL_4 - Region 1 - cyclic-lactone-autoinducer

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Location: 72,718 - 93,441 nt. (total: 20,724 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaICLENCNL_5 - Region 1 - ranthipeptide

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Location: 86,180 - 108,028 nt. (total: 21,849 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

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gnlProkkaICLENCNL_6 - Region 1 - cyclic-lactone-autoinducer

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Location: 54,728 - 75,474 nt. (total: 20,747 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

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gnlProkkaICLENCNL_10 - Region 1 - cyclic-lactone-autoinducer

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Location: 12,500 - 33,248 nt. (total: 20,749 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaICLENCNL_11 - Region 1 - RRE-containing

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Location: 211,836 - 232,087 nt. (total: 20,252 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaICLENCNL_19 - Region 1 - NRPS,cyclic-lactone-autoinducer

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Location: 4,033 - 57,510 nt. (total: 53,478 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 4032 - 57510:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg19_15

Search NORINE for peptide: strict or relaxed

AMP-binding (39..445): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVLSK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (79% 8Å match); Sal PLPAQGVLNK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg19_16

Search NORINE for peptide: strict or relaxed

AMP-binding (553..948): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSMIWK
Nearest Stachelhaus code(s):: Cys DLYNMSMIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1975..2374): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg19_18

Search NORINE for peptide: strict or relaxed

AMP-binding (507..899): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaICLENCNL_32 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 20,333 nt. (total: 20,333 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.