GCA_015667495.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaBHDCKIGM_48 (original name was: gnl|Prokka|BHDCKIGM_48)

Region	Type	From	To	Most similar known cluster		Similarity
Region 48.1	NRPS	8,889	40,922	dipeptide aldehydes	NRP	23%

gnlProkkaBHDCKIGM_49 (original name was: gnl|Prokka|BHDCKIGM_49)

Region	Type	From	To	Most similar known cluster		Similarity
Region 49.1	ranthipeptide	2,636	24,224

gnlProkkaBHDCKIGM_55 (original name was: gnl|Prokka|BHDCKIGM_55)

Region	Type	From	To	Most similar known cluster		Similarity
Region 55.1	cyclic-lactone-autoinducer	1	19,789

gnlProkkaBHDCKIGM_68 (original name was: gnl|Prokka|BHDCKIGM_68)

Region	Type	From	To	Most similar known cluster		Similarity
Region 68.1	lanthipeptide-class-ii	1	21,270

Compact view

gnlProkkaBHDCKIGM_48 - Region 1 - NRPS

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Location: 8,889 - 40,922 nt. (total: 32,034 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 8888 - 40922:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg48_27

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaBHDCKIGM_49 - Region 1 - ranthipeptide

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Location: 2,636 - 24,224 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaBHDCKIGM_55 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 19,789 nt. (total: 19,789 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaBHDCKIGM_68 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 21,270 nt. (total: 21,270 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg68_9

allorf_00097_00282

Leader:

MSLNFSTYAYITKSPVRYSDAAVFSA

Core:

AAPDYHHPELFPVLLLFHTRELYPHMTEVTCFQTL

Core with dehydrations:

AAPDYHHPELFPVLLLFHDhbRELYPHMDhbEVDhbCysFQDhbL

antiSMASH-DB 4.0 matches for allorf_00097_00282

14.3% NZ_CP058552.1: Lanthipeptides: allorf_1481579_1481740_lanthipeptide

23

YPHMTEVTCFQTL

35

Y H+ + C QT+

6

YKHVQKTNCVQTI

18

allorf_13796_13876

Leader:

MTGKKHNREKSVKGA

Core:

SGTACGGAALD

Core with dehydrations:

DhaGDhbACysGGAALD

antiSMASH-DB 4.0 matches for allorf_13796_13876

21.9% NZ_JACHCP010000005.1: Lanthipeptides: allorf_355089_355289_lanthipeptide

3

TACGGAALD

11

TACG ALD

5

TACGFVALD

13

17.6% NZ_KB235948.1and 1 other:

NZ_KB235948.1 Lanthipeptides: allorf_4821767_4821901_lanthipeptide

Lanthipeptides: allorf_4821767_4821901_lanthipeptide

1

SGTACGGAAL

10

SG+ CGG +L

7

SGSTCGGKSL

16

allorf_18243_18344

Leader:

MNTRTLYFS

Core:

RCRNRPHWSRVPPFGSTTTYEEWA

Core with dehydrations:

RCysRNRPHWDhaRVPPFGDhaDhbDhbDhbYEEWA

antiSMASH-DB 4.0 matches for allorf_18243_18344

18.4% NC_013131.1: Lanthipeptides: allorf_05045649_05045855_lanthipeptide

1

RCRNRPHWSRVPP

13

RC R H S+ PP

10

RCSRRSHGSQSPP

22

15.0% NZ_JACKVL010000013.1: Lanthipeptides: allorf_1185207_1185365_lanthipeptide

8

WSRVPPFGS

16

WSR PP S

31

WSRTPPLAS

39

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_00097_00282 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 4093.1 Da

Molecular weight: 4095.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4113.7 Da N = 1; 4131.7 Da N = 2; 4149.7 Da N = 3

allorf_13796_13876 - Class II

Cleavage pHMM score: -2.90

RODEO score: 0

Monoisotopic mass: 885.4 Da

Molecular weight: 885.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 904.0 Da N = 1

allorf_18243_18344 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 2842.4 Da

Molecular weight: 2844.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2862.1 Da N = 1; 2880.1 Da N = 2; 2898.2 Da N = 3; 2916.2 Da N = 4