LOCUS       c00231_gnlPro..        15420 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OMKNHDFD_231.
ACCESSION   c00231_gnlPro..
VERSION     c00231_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 02:44:56
            Original ID  :: gnl|Prokka|OMKNHDFD_231
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15420
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15420
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[97:5420]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      98..5420
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..15420
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15420
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             98..1534
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_1"
                     /sec_met_domain="CT_Vparvula (E-value: 2e-136, bitscore:
                     445.7, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNATQSLAGTRITSLLDANSFVEIGQGVTARSTDFNMTANKAPS
                     DGVITGYGVIDDKLVYVYSQDASVLNGTVGEMHAKKITRLYDLAMKTGAPVIGLVDCAG
                     IRLQEATDALEAFGQIYLKQTLASGVIPQITAIFGTCGGGMAVIPSLTDFTFMESKKGK
                     MFVNTPNALEGNNTDKCDTAAADFQSKETGVIDGVGTEDEILGQIRSLVSLLPSNNEDT
                     DNYTECTDDLNRVCADLANCAGDTAIALSQIADNGEFFETKADYAKDMVTGFIRLNGAT
                     VGAVANRSEVYDAEGKKTETFDGSISARGARKAADFVKFCDAFDIPVLTLTNATGFMAT
                     LCSEKMMAKSVGELVAAFADATVPKVNVIIGKAYGTAYVAMNSKSIGADLVYAWDNAEI
                     GMMDASLAAKIMYADADAAELNEKAAQYRELQNGVASAAARGYVDTVISPADTRKYVIG
                     AFEMLFTKREDRPSKKHGTV"
     CDS             1549..2304
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_2"
                     /sec_met_domain="OAD_gamma (E-value: 1.3e-27, bitscore:
                     86.6, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMIKKITSVFMTFLVMAALVIGSTSVVFAADKVDDTVKSTLVTT
                     AEGLTDAIVQLKDEEIENYMSSGDDFTTSAMQSWQTSKDELGAKKDSNGETTVTFKDDQ
                     YTVTVPLKFEKAEANFVYVFDSQGTPTSMSVDVQYGMGKTLQRAGLNTLMGIGTVFVML
                     ILLSLLISLFRFIPNPEAKKAAEAKAAKAAKEAEAAAIAQTAPAQAEENLADDGELVAV
                     IAAAIAAAEGTTTDGFVVRSIRKVKRNRR"
     CDS             2332..2700
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 63.1; E-value:
                     5.3e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.6e-20, bitscore:
                     63.1, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYTITVNGTAYEVTVEETGSVSAPAAAPKAAPKAAPAAAPKAA
                     APAAGAGAVKVTASVPGKVVKVAASVGQAVKAGDSVIILESMKMEIPVVAPQDGTIASI
                     DVAEGASVENGDTLATMN"
     CDS             2717..3865
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_4"
                     /sec_met_domain="OAD_beta (E-value: 8e-157, bitscore:
                     511.9, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYVTETLSNLIHQTAFFNLTWGNYLMIAVACVFLYLAIKKGFEP
                     LLLVPIAFGMLLVNIYPDIMANPEDMSNGVGGLLHYFYILDEWSILPSLIFMGVGAMTD
                     FGPLIANPISFLMGAAAQLGIYAAYFLAIFLGFNGKAAAAISIIGGADGPTSIFLAGKL
                     GQSALMGPIAVAAYSYMSLVPIIQPPIMKLCTTEKERKIKMDQLRPVSKLEKILFPIVI
                     TIVVCLILPTTAPLVGMLMLGNLFRECGVVKQLTETASNALMYIVVILLGTSVGASTSA
                     EAFLNADTLKIVVLGLVAFAIGTFGGCMLGKLLCKLTHGKINPLIGSAGVSAVPMAARV
                     SQKVGAEADPTNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     CDS             4008..5420
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_5"
                     /sec_met_domain="HMGL-like (E-value: 6.8e-47, bitscore:
                     150.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 7.6e-59, bitscore:
                     189.1, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELEKKPVKIVETILRDAHQSQIATRMTTEQMLPIVDKLDKVGY
                     HAVECWGGATFDASLRFLHEDPWERLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVVE
                     YFVQKSAANGIDIIRIFDCLNDLRNLQTAVSAANKEKAHAQVALSYTLGDAYTLEYWTD
                     IAKRIEDMGADSICIKDMAGLLLPNKATELVTALKETVKIPIDLHTHYTSGVASMTYLK
                     AVEAGVDIIDTAMSPFALGTSQPATEVMVETFKDTPYDTGFDQKLLSEIADYFRPIRDE
                     ALDSGLLNPKNLGVNIKTLLYQVPGGMLSNLTSQLKEQHAEDKFYDVLEEVPRVRKDLG
                     EPPLVTPSSQIVGTQAVFNVLMGERYKMVTKETKDILLGKYGATVKPFNPEVQKKCIGD
                     EKPITCRPADLLDNELEKLESEMAQYKEQDEDVLTYALFPQVAMDFFKYRQAQKTKVDE
                     KAADKKNGAYPV"
     CDS             5541..6992
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HI0933_like"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg231_6"
                     /sec_met_domain="HI0933_like (E-value: 1.2e-128, bitscore:
                     419.5, seeds: 46, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VVHIYIDRSEAETWNHDEIDNLQGKINTGEYSMSKVIIIGGGAAG
                     MMAGVFAARNHHEVHILEKNEKLGKKVFITGKGRCNVTNACDTEELFPAMMSNPKFLYS
                     SFYSFTPQDVMEFFEKAGVPLKVERGNRVFPQSDHSSDIIRALERELKKAGAKIHLHTA
                     VQEIVKKPVTESVTDSVNTLESEAALTESGSDAGKSRKGKKSSDIQQEKITGVILTDGT
                     FIEGDAVIVATGGFSYQSTGSTGDGYRFARELGLKVTDIAPSLVPLKTKEDYIPKLQGL
                     SLKNTGLTIKNGKKVLYEDFGEMMFTHFGVTGPMILSASAHIGAKLAKAPNGELSAYLD
                     LKPALTREQLDARILREFEAGPNKQFKNVIGVLFPSSLTPVMLELGGIPAEKKIHDISR
                     EERQHFIDLIKAFPFTITGMGEFKEAIITRGGVSVKEINPGTMESKKISGLYFAGEVLD
                     LDAVTGGYNLQIAWSTAYLAAQAIQ"
     CDS             7147..7818
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cytidylate_kin"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg231_7"
                     /sec_met_domain="Cytidylate_kin (E-value: 1e-77, bitscore:
                     250.9, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGCNIAIDGPAGAGKSTIAKKVAKELSFIYVDTGAMYRAMALYLL
                     NHGVNGENQEEIEAVCSGADISIEYKNGEQIVILNGENVNAMIRTEQVGNMASKSSANP
                     KVRAHLLKLQRTLAEKNDVVMDGRDIGTTILPNAEVKIYLTASADTRAKRRALEYEQKG
                     EAFDLDQIRKDIIERDERDMNREISPLKQADDAVLVDSSEMGIDDVVNAILNVYKEKVQ
                     K"
     CDS             7975..8853
                     /locus_tag="ctg231_8"
                     /transl_table=11
                     /translation="MKVKVAETAGFCFGVKRAVDKVYELIGTEQKPIYTLGPIIHNEGV
                     VADLEARGVHVITEADLDSPDETLQNGTVVIRSHGVGKPIYDKLKEKNISYVDVTCPFV
                     LKIHRIVEKESLAGNHIIIIGDKDHPEVQGICGWCQGPYTVIRNKEEAEAFVPPKGKKI
                     SIVSQTTFNYNKFKDLVEILCKKRYDNNVLNILNILNTICNATEERQREAKNIAGEVDT
                     MLVVGGRHSSNTQKLFEICKKECGNTYYIQTPVDLDSEMFQCSSYVGITAGASTPNKII
                     EEVQEHVRIKF"
     CDS             8834..9910
                     /locus_tag="ctg231_9"
                     /transl_table=11
                     /translation="MSELSFEQMLEDSVKTIRNGEIVQGTVIDVKEDEIILNIGYKADG
                     IITKNEYSNDASLVLTDAVHVGDTMEAKVLKVNDGEGQVTLTYKRLAAEKGNKRLEEAF
                     ENQEVLKAPVTQVLDGGLCVNVDEARVFIPASLVSDTYEKDLSKYADQEIEFVITEFNP
                     RRRRIIGNRKQLLLAEKAEKQKELMERIHVGDTVEGTVKNVTDFGAFIDLGGADGLLHI
                     SEMSWGRVENPKKVFTVGDKVKVLIKEINGEKIALSLKFPEANPWLTAAEDFAVGNVVK
                     GKVARMTDFGAFVELAPGVDALLHVSQISREHVAKPADVLSIGQEIEAKVVDFNGEDKK
                     ISLSMKALEAPAEEATEE"
     CDS             10058..10804
                     /locus_tag="ctg231_10"
                     /transl_table=11
                     /translation="MSIKGIYDIHCHIVPGVDDGATDIGETVKLLRMEYEQGVRTVIAT
                     PHFRFQMFETPAEKVREQFRLVEKAASEISPDLHVYLGCEFHANMEMLPMLREQKVMTM
                     AGSRYVLTEFSHNSEESYIRERLGALLSGGYKPIMAHIERYEATRNSLDFVEELADMGV
                     YMQINADSITGKDGFFTKRYCNKIMKDGLLHFVGSDCHNSAKRSSRIGEAYRMVSAKFG
                     QDYADELFIHNPAEILKQKQKHEATD"
     CDS             10945..11724
                     /locus_tag="ctg231_11"
                     /transl_table=11
                     /translation="MLDNLNSNFESSKAQRPDANDDEVEIDLLEIFYALKKKILLVLMV
                     ALVGGCIAAAYTQFLMTPIYSSTSSILVLSKETTLTSLADLQLGASLTSDYTVLITSTP
                     VMEQVISDLDLDMTAEQLKGSVSINNPTDTRILEITVNNTDSKMAKKIVDEIANVSSSY
                     IGDKMEVIPPKIIEVGKIATVRTSPSVKKNAALGFLLGFLACAAIVVVYAVMDDTIKTE
                     EDIEKYLGVSVLAKVPDRKDFINSKNRKSKNKNKKHH"
     CDS             11746..12456
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CbiA"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg231_12"
                     /sec_met_domain="CbiA (E-value: 5.5e-09, bitscore: 26.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLNLTDKRNPDYFYSEAIKTLRTNIQLSGQSIKTILVTSCYPN
                     EGKSDIVLSLAQELGSIGKKVLLLDADIRKTAYAGRLGVEEEVKGLSQLLSGQVGLQEV
                     IYSTNFPNMDIIFGGPNAPNPSGLLSENIFKVFLKEIREYYNYILIDTPPIGTVIDAAV
                     IGRCCDGAVFLIEPGNVRYRDAQKAFKQLERSGCRILGAVMNKIDTSDDKYYSSYYKHY
                     GEYYRRSDEEAQIK"
     CDS             12520..13482
                     /locus_tag="ctg231_13"
                     /transl_table=11
                     /translation="VSKIRNKKIKKTNILLGFICILLAVLLGFILVTSYKSEQKESRHL
                     SEIADDQQSGIEDYEAVKERAKELEEVSSEEAEDTEDIKSKNTEDTKDTEKTDTADTED
                     ADKTEDTKDKTTAASGIVCWGDDLINGEESNTYSYMTVLQKLLTDNGYNMTVVNKTLQG
                     GGTLSMMKMAGVSDETIQSYITRHQQAANGAQLNVTETGIRDLTEEQTTRNDMDCIPVI
                     FMGYYGGWNHDPAELADQQEQILNTFQNKDQFIVVGTRPMDGSVTSEALDQVLSQKWGE
                     HYISLADVTAQPSSTYEAQQAMAEAILQKLQELNYISKN"
     CDS             13519..14550
                     /locus_tag="ctg231_14"
                     /transl_table=11
                     /translation="MKKTTRKAIRQRKICMLLIIILIIVLSILGGGYYLLSQKNAASSK
                     AGGQNSDSRNQTDLNQNSDTVEYKGEIYKYNNHLSNYLFLGIDTREAVDTYQSQADAGQ
                     ADAIFLVSMDRATEKIKVLFIPRDSMTRIEVFNPYGQSLGETTDHLNIQYAFGDGKEKS
                     CELMKTAVSDMLDGLPIQGYCSMNMDGISVITDFVGGIQLTIPDDSLADVNSEYKKGAV
                     VDITGETAEQFVRYRDIDKTQSALVRQERQKTFLQALVQKAQEKAGEDAGFVTGLYDSV
                     KSYTVTNMGNDIFAKLLAASQNGITDTETVPGEGTHGENFDEYHIDEDALSDLIISMFY
                     EKI"
     CDS             14691..15317
                     /locus_tag="ctg231_15"
                     /transl_table=11
                     /translation="MKIAKKFMTLALVAALGVGCATVGVGAAPSNTSDLAAVAPSKTSE
                     ITVDSKQSSIYEVVKELEKTQGFADLQAKYKDLADAFKKINDGTMKMEDFIKVLKSLSV
                     DEADKADLEDAIAKLEGKMIVTFVYEFNVLKPEEAKPNDDGTYEVKLNVPSLTDTMEGI
                     QLWVYSKDGTKKFVVIDPVNIDKEGKTLTVTLNDGDFFMVIADAK"
ORIGIN
        1 aatgttgaaa ttttctgccg gaaaaggtaa aattaaaatc ggctaaattt ttgggttaac
       61 ttttaaatat tctcataaag atggagggca aacaagaatg agtaatgcaa cacaaagctt
      121 agcaggcaca agaattactt ctttgcttga cgcaaacagt tttgtcgaaa tcggacaggg
      181 tgtaacagcc agatccacag atttcaacat gactgcaaac aaggcaccat ctgacggtgt
      241 tatcaccgga tatggtgtga tcgatgacaa actggtttat gtatacagcc aggatgcatc
      301 tgtattaaac ggtactgttg gtgaaatgca tgcaaagaag atcacaagac tttatgatct
      361 tgccatgaaa acaggcgctc cggttatcgg acttgttgac tgtgcaggta tccgtcttca
      421 ggaagcaaca gacgcactgg aagctttcgg acagatctat ctgaaacaga cactggcatc
      481 cggtgtgatc ccgcagatca cagcaatctt cggaacatgc ggcggtggta tggcagttat
      541 cccgtccctg acagatttca cattcatgga atccaaaaaa ggaaaaatgt tcgtcaacac
      601 accaaacgca ctggaaggaa acaacacaga caaatgcgac acagcagcag ctgatttcca
      661 gagcaaagag acaggtgtga tcgatggcgt tggtacagag gatgagattc ttggccagat
      721 ccgttcctta gtatcacttc ttccttctaa taatgaagac acagacaatt acacagagtg
      781 tacagatgat cttaaccgcg tatgcgcaga tcttgcaaac tgtgcaggtg acacagcaat
      841 cgcactttcc cagatcgctg acaacggtga attcttcgag acaaaagcag actatgcaaa
      901 agacatggtt acaggcttta tccgtttaaa cggcgctaca gtaggtgctg ttgcaaacag
      961 aagcgaagta tatgacgcag aaggtaagaa aacagaaaca tttgacggaa gcatctctgc
     1021 aagaggcgca agaaaagcag cagatttcgt gaaattctgc gacgcttttg atattccggt
     1081 acttacactt acaaacgcaa caggcttcat ggcaacctta tgcagcgaga agatgatggc
     1141 gaaatccgta ggtgaactgg tagctgcatt cgcagatgca actgttccga aggtaaatgt
     1201 gatcatcggc aaagcttacg gtacagctta cgtggcaatg aacagcaaat ctatcggtgc
     1261 agaccttgta tatgcatggg ataacgcaga gatcggtatg atggatgctt ccctggcagc
     1321 caagatcatg tatgcagatg cagatgctgc tgaactgaat gagaaagctg ctcagtacag
     1381 agagcttcag aatggtgttg cttccgcagc agcaagaggc tatgttgaca ctgtgatcag
     1441 cccggctgac acaagaaaat atgtaatcgg tgcatttgag atgctgttca caaagagaga
     1501 agatcgtccg tctaagaaac atggaacggt ctaagcgggg tgacgcatat gaaaatgatc
     1561 aaaaaaataa cttctgtatt catgacattt ctcgtaatgg ctgcgctggt gatcggcagc
     1621 acctctgtgg tatttgccgc agataaggtt gatgatactg ttaagtccac actggtaact
     1681 acagcagaag gactgacaga tgccattgtc cagcttaaag atgaagaaat cgagaattac
     1741 atgagttcag gagatgactt tacaacaagt gccatgcagt cctggcagac atccaaggat
     1801 gaacttggcg caaagaagga cagcaatggt gaaactactg taactttcaa agatgatcag
     1861 tacacagtta ctgttcctct taaatttgag aaagcagaag caaactttgt atatgtcttt
     1921 gactcacagg gaacaccgac ttccatgtct gtagatgtac agtatggtat gggcaagact
     1981 cttcagagag caggccttaa tactttgatg ggtattggaa ctgtattcgt aatgctgatt
     2041 ttattaagcc ttctgatttc tctgttcaga tttattccga atccggaagc aaagaaagca
     2101 gcagaagcaa aagcagccaa agctgcaaaa gaggcagagg cagcagccat cgcacagaca
     2161 gcacctgcac aggcagaaga gaaccttgca gatgacggag agctggtagc tgttatcgca
     2221 gcagccatcg cagcagcaga aggaactaca acagatggct tcgtagttcg ttccatccgt
     2281 aaagtaaaaa gaaatagaag ataattgtca tttatttagg aggattttaa aatgaaaagc
     2341 tatacaatta cagtaaatgg tacagcatat gaagtaactg ttgaagaaac aggaagcgta
     2401 tccgctcctg cagcagctcc aaaagcagca cctaaggctg cacctgcagc agctccgaaa
     2461 gcagcagctc ctgcagcagg cgccggcgca gtgaaagtaa ctgcttccgt acctggtaaa
     2521 gttgtcaaag tagcagcaag cgttggacag gcagtaaaag ccggagacag cgtaattatt
     2581 cttgaatcca tgaaaatgga aatccctgta gtagctcctc aggacggtac tatcgcaagc
     2641 attgatgttg cagaaggtgc ctctgtagaa aacggagata ctttagcaac aatgaactaa
     2701 gacgggaggt agtcaaatgt cttatgtaac agaaacattg tccaacctga ttcaccagac
     2761 agcattcttc aatctgacat ggggcaatta cctgatgatc gcagtagcct gcgtattcct
     2821 ttatcttgct atcaagaagg gattcgagcc gcttctgctt gttcctatcg cattcggtat
     2881 gctgcttgta aatatctatc cggacattat ggcaaacccg gaagatatgt ccaatggtgt
     2941 aggtggactt cttcattatt tctacatcct tgatgagtgg agtatcttac catccctgat
     3001 cttcatgggt gtaggtgcca tgacagactt cggtccgctg atcgcaaacc cgatcagctt
     3061 cctgatgggt gctgcagcac agcttggtat ctatgcagct tacttccttg caatctttct
     3121 tggattcaac ggaaaagcag cagcagctat ctccatcatc ggtggtgctg acggcccgac
     3181 ttccattttc cttgcaggta aacttggaca gtctgcactg atgggaccga tcgcagtggc
     3241 agcatattcc tatatgtcac tggttccgat catccagccg cctatcatga aactctgcac
     3301 aacagagaaa gaacgtaaga tcaaaatgga tcagcttcgc ccggtttcca aactggagaa
     3361 gatcctgttc ccgatcgtta tcacaatcgt tgtctgcctg attttgccta caacagctcc
     3421 gctggttggt atgctcatgc ttggtaacct gttcagagag tgcggtgttg taaaacagct
     3481 gacagaaaca gcttccaatg ccctgatgta tatcgtagtt atccttctgg gtacatccgt
     3541 aggtgcttcc acaagtgcag aagcattctt aaacgcagat actctgaaga tcgtagtttt
     3601 aggtcttgtt gcattcgcaa tcggtacatt cggcggatgt atgcttggta aactgctctg
     3661 caaactgact catggaaaga tcaacccgct gatcggttcc gcaggtgtat ccgcagtacc
     3721 tatggcagcc cgtgtatccc agaaagtggg tgcagaggca gatcctacaa acttccttct
     3781 gatgcacgca atgggcccga acgttgccgg tgttatcggt acagccgtag cagccggaac
     3841 attcatggct attttcggag tataaccaaa tcaagcaagc tatcttgctt gtatcaggag
     3901 gagtgcaact ccttctgata agctgcggag cagctatttg gttatgagca agtagcgaag
     3961 cggattgcga atatccgatt aaacagaact ataaaggaga tataataatg gcagaattag
     4021 agaaaaaacc tgttaaaatt gtggaaacca ttctccgtga tgctcatcag tcccagatcg
     4081 caacacgtat gaccacagaa cagatgcttc cgatcgtaga taaacttgat aaagtcggct
     4141 atcacgcagt agagtgctgg ggtggtgcaa cattcgatgc atcccttcgt ttccttcacg
     4201 aagatccatg ggaaagactt cgtaaattac gtgacggctt caaaaacaca aaacttcaga
     4261 tgcttttccg tggacagaac atccttggat accgtccata cgcagatgac gttgtagaat
     4321 acttcgtaca gaaatctgca gcaaacggaa tcgacatcat ccgtattttt gactgcctca
     4381 atgaccttcg taacctgcag acagcagtaa gcgctgcaaa caaagagaag gctcatgcac
     4441 aggtagctct ttcctacaca ttaggagatg cttacacact ggaatactgg acagacattg
     4501 caaaacgtat tgaagatatg ggcgctgatt ccatctgtat caaagatatg gcaggtcttc
     4561 tccttccaaa caaagctaca gagcttgtaa cagcattaaa agaaacagtt aagattccaa
     4621 tcgaccttca tacacattac acctcaggtg tagcttccat gacttactta aaagcagtag
     4681 aagcaggtgt ggatatcatc gatacagcaa tgtctccatt tgcactggga acatcccagc
     4741 ctgcaacaga ggttatggtt gagacattca aggatacacc atacgacaca ggatttgatc
     4801 agaagcttct ctccgaaatc gctgattact tccgtccgat tcgtgacgaa gctcttgaca
     4861 gcggactgct caatccgaag aaccttggtg ttaacatcaa aactttactg taccaggtac
     4921 cgggcggtat gctctccaac ctgacttccc agctgaaaga acagcacgca gaagacaaat
     4981 tctacgatgt tctcgaggaa gttccgcgtg tacgtaaaga ccttggtgaa ccgccacttg
     5041 ttactccgtc ttcacagatc gttggtacac aggctgtatt caacgtactt atgggcgaga
     5101 gatacaaaat ggttacaaaa gaaacaaaag atatcctgtt aggaaaatac ggcgcaactg
     5161 taaaaccgtt taatccggaa gtacagaaga agtgcatcgg cgatgagaaa ccgatcacat
     5221 gccgtcctgc agaccttctt gacaacgagc ttgagaaact tgagagcgaa atggctcagt
     5281 acaaggaaca ggatgaagac gtattaacat acgcactgtt cccacaggta gctatggact
     5341 tcttcaaata ccgtcaggct cagaaaacaa aagttgacga gaaagcagct gacaaaaaga
     5401 acggagcata tcctgtataa ttgatcagta attaaatatg tccaaacgaa aaaaggagct
     5461 tgcgcaagca ggctcctttg cgttattatg aaagaatatg atacccggga atataatgtg
     5521 ccaatgggga tatgctgcca gtggtacata tctatattga tcgtagcgaa gcggagacgt
     5581 ggaaccatga tgaaatagac aatctgcagg gtaaaataaa tacaggagaa tattccatga
     5641 gcaaagttat aataatcggc ggcggagccg ccggaatgat ggcaggggtt tttgcagccc
     5701 gtaaccatca tgaagttcat atattagaga agaacgagaa actgggaaag aaagtcttca
     5761 tcacaggaaa aggcagatgc aatgtaacaa atgcctgcga cacagaagaa ctctttcctg
     5821 caatgatgag caatcccaag ttcctctaca gcagtttcta ttcatttacc cctcaggatg
     5881 taatggagtt ctttgagaaa gcaggtgtcc ctctgaaggt agagcgtgga aacagagtat
     5941 tcccacagtc tgaccattct tcagacatca tccgcgctct ggagcgtgag ctgaagaaag
     6001 caggagcaaa gattcatctt cacactgctg tgcaggaaat cgtaaagaaa cctgtaacgg
     6061 aatctgtaac agattccgta aatacccttg aatcagaagc cgcattaaca gaatcaggat
     6121 ctgatgcagg aaaaagcaga aaaggaaaaa aatcatctga catacagcag gagaagatca
     6181 caggagttat acttacagat ggtactttca tagaaggcga tgcagtcatc gtggcaaccg
     6241 gcggattctc ttatcagagt acaggctcca caggtgacgg ataccgcttc gcaagagaac
     6301 tgggactgaa agtcacggac attgctccct cccttgtgcc attaaagaca aaagaagact
     6361 atatccctaa gcttcaggga ctttccctta agaatacagg acttaccata aagaatggaa
     6421 agaaagtact ttacgaggat ttcggagaga tgatgttcac ccatttcgga gtcacaggtc
     6481 caatgatcct ctccgccagt gcccacatcg gtgcaaaact ggcaaaagca ccaaacggcg
     6541 aactgtccgc atatctggat ctgaagcctg cactcaccag agaacagctg gatgccagga
     6601 tcctgcgtga atttgaagca ggaccaaaca aacagttcaa gaacgtgatt ggcgtacttt
     6661 tcccgtcatc tctgactccg gtaatgctgg aactgggtgg aatccccgca gagaagaaaa
     6721 tccacgatat ctccagagaa gaacgccagc acttcattga ccttatcaaa gctttcccct
     6781 tcacaatcac aggaatgggt gagttcaaag aagccatcat caccagaggc ggcgtttctg
     6841 tcaaagagat caaccccgga acaatggaat caaagaaaat ctcaggtctt tacttcgcag
     6901 gcgaggtact ggatctggat gcagtgacag gtggatataa tttacagatt gcctggtcta
     6961 ccgcatatct ggcagcgcaa gctatacagt aagctgaaac aagcagatga tgagtaccag
     7021 taccttgtag gtggattata tctatgatat gattcagcta tacgttattt agcgattcat
     7081 aaacatcaat caataataaa acagagaaaa tctgtttgaa taatagaaac aggaggaacc
     7141 acgaaaatgg gatgtaacat agccatcgac ggacctgcag gagcaggaaa gagcaccatc
     7201 gcaaagaaag tagcgaaaga attatcattt atctatgtag atacaggagc catgtacagg
     7261 gcaatggctt tatatctttt gaaccatggc gtaaacggag aaaatcagga agagatcgaa
     7321 gctgtatgtt ccggcgctga catctccatc gaatacaaaa acggagaaca gattgtcatc
     7381 ctcaacggag agaacgtaaa tgctatgatc cgcaccgaac aggtaggaaa catggcatcc
     7441 aaaagctccg caaacccgaa agtcagagcc catcttctga aacttcagag aactctggca
     7501 gagaaaaacg acgtagtcat ggacggcaga gatatcggca caaccatcct tccaaacgca
     7561 gaagtaaaaa tctacctcac agcaagcgca gacaccagag caaaaagaag agcactggaa
     7621 tatgaacaga agggagaagc ctttgacctg gaccagatcc gaaaagacat catcgagcgc
     7681 gacgaacgcg acatgaaccg cgaaatctcc cctctgaaac aggcagacga cgcagtgctt
     7741 gtagattctt cggaaatggg aattgatgat gttgtcaatg caattcttaa tgtatataaa
     7801 gaaaaggtgc agaaatgatc agataactgc ctgtagttca tcagaagatg acacgcgcaa
     7861 agatatctga cacaggattt cacgctctga tgagttggat taagatacat tatttcagag
     7921 gattgctata aattccagaa gaatggcaat gaatataaat taagaggatt agtaatgaaa
     7981 gtaaaagtag cagaaacagc aggcttctgc ttcggcgtca aacgggcagt agacaaagtc
     8041 tacgaactga tcggcacaga acagaaacca atctacactc tgggtccaat catccacaat
     8101 gaaggcgtag tagcggatct ggaagcccgt ggtgtccatg ttatcacaga agcagacctg
     8161 gattccccgg atgaaactct gcagaacgga acagtagtca tccgctccca cggcgtaggc
     8221 aaacccatct acgataagct gaaagaaaaa aatatctcct acgtagatgt cacctgcccc
     8281 tttgtcctga agatccacag aatcgtggaa aaagaaagcc ttgcaggaaa ccacatcatc
     8341 atcatcggcg acaaagacca ccccgaagta caaggcatct gcggctggtg tcagggaccg
     8401 tacacggtga tcagaaacaa agaagaagcc gaagcatttg tacccccaaa aggcaaaaaa
     8461 ataagtattg tgtcccagac gacatttaat tacaacaaat ttaaagattt agttgaaatt
     8521 ctttgcaaaa agaggtatga taataatgtt ttaaatattc tcaatatttt aaacactatt
     8581 tgtaatgcta ccgaagaacg gcagagagaa gcaaaaaaca tagccggaga ggtagacact
     8641 atgttggtcg taggtggccg gcacagttcc aatactcaaa agctgtttga aatatgtaaa
     8701 aaggaatgtg gaaatactta ctatatacaa acacctgtag acttggattc tgaaatgttc
     8761 caatgcagta gttatgtagg tattacagca ggggcttcca ccccaaacaa aattattgag
     8821 gaggttcaag aacatgtcag aattaagttt tgaacaaatg ctggaagatt ccgtaaagac
     8881 tatcagaaat ggagagattg tacagggtac tgtcatcgat gtaaaagaag atgagattat
     8941 tctgaatatc ggttataaag cagacggtat tatcacaaag aacgaatact ctaacgatgc
     9001 aagccttgta ctcacagatg ctgttcacgt tggagacaca atggaagcaa aagttctcaa
     9061 agtaaacgat ggagaaggtc aggttactct tacttacaag agactggcag cagagaaagg
     9121 caacaaacgt cttgaagaag catttgagaa tcaggaagta ttaaaagctc ctgtaactca
     9181 ggtacttgac ggcggacttt gtgtaaacgt agacgaagca agagtattca ttcctgcaag
     9241 cctggtttcc gacacatacg agaaagacct ttccaaatat gcagatcagg agatcgaatt
     9301 cgttatcaca gaattcaacc cgagaagacg ccgcatcatc ggtaaccgta agcagcttct
     9361 tcttgctgag aaagcagaga aacagaaaga attaatggag agaatccatg ttggcgatac
     9421 agttgaagga acagttaaga acgttacaga tttcggtgca ttcatcgatc tgggcggagc
     9481 agacggactt cttcacattt ccgaaatgtc ttggggcaga gtagagaatc cgaagaaagt
     9541 attcactgtt ggcgataaag tcaaagttct tatcaaagag atcaacggag agaagatcgc
     9601 actttccctt aaattcccgg aggcaaatcc atggctcaca gcagctgagg atttcgctgt
     9661 aggaaacgta gttaaaggta aagttgcacg tatgacagac ttcggtgcat tcgtagagct
     9721 tgcacctggt gtagatgcac ttcttcacgt atcccagatt tccagagagc acgttgccaa
     9781 acctgcagat gtactgtcaa tcggacagga gatcgaagct aaggttgttg acttcaacgg
     9841 cgaagacaag aagatcagcc tgagcatgaa agctcttgag gcaccggctg aggaagctac
     9901 agaagaataa ttaagagaat actcataaat attcagcaac cggctttgta tgaggatgat
     9961 catacagagc cggttgattg ttttttcaga ttacgaatgt ggtcagcagt tacagaagca
    10021 tcagaaatgc tgcagacagt caggaaggaa tgataggatg tccataaaag gaatttatga
    10081 tatacactgt catattgtac ccggtgtaga cgatggagcc acagatatag gagaaaccgt
    10141 aaaattactc cgaatggaat atgaacaggg cgtgcggacg gttattgcaa ctccccattt
    10201 ccgctttcag atgtttgaaa cacctgcgga gaaagtcaga gagcagttca gactggtgga
    10261 gaaagcggca tcagagataa gcccggatct ccatgtgtat ctgggatgtg agtttcacgc
    10321 caatatggag atgcttccca tgctcagaga acagaaagta atgacaatgg ccggttcgcg
    10381 gtatgttctt acagaatttt cccataactc ggaagaaagc tatatacggg aacgtctcgg
    10441 tgcattgctc tccggaggat ataagccgat catggcacat atagaacgtt acgaagccac
    10501 acgcaacagt ctggattttg tggaagaact ggcagatatg ggcgtctata tgcagattaa
    10561 tgcagacagc atcacaggaa aagacggatt ttttactaaa cggtactgta ataaaatcat
    10621 gaaagacgga ctccttcatt tcgtcggatc agactgtcac aacagtgcaa agaggagcag
    10681 caggattgga gaggcatatc gtatggtttc agcgaaattc gggcaggact atgcagatga
    10741 actgtttatt cataatcctg cagaaattct gaaacagaaa caaaaacatg aggctacaga
    10801 ttgacttttt ttattggctg atatacaata aaatcggttt gtgtcaattt tgctgtttac
    10861 tttcagaaat acataagaca gaatattacc tgtgaacagt aatgtacaaa actataaaga
    10921 aaaaagggaa aaggaaatta tcagatgtta gacaatttga acagtaactt tgagagcagc
    10981 aaagcacaga gacccgatgc aaatgatgat gaagtcgaaa tcgacttact ggaaatattt
    11041 tatgcgctca agaaaaaaat ccttctggta ctgatggttg ctcttgtcgg aggctgtatt
    11101 gcagcagcat atacgcagtt tttaatgaca ccgatctaca gctccacatc cagtatcctt
    11161 gtactttcga aagaaaccac actgacatca ctggcggatc tgcagttagg tgccagcctg
    11221 accagtgatt atacagttct gatcacaagt acccctgtga tggaacaggt gatttcagat
    11281 ttggatctgg atatgactgc agaacagctg aaaggaagcg taagtatcaa taatcctaca
    11341 gatacccgta tccttgagat tactgtcaat aatacagatt ctaaaatggc aaagaagatc
    11401 gtagatgaga ttgcgaatgt ttcttcatcc tatattggcg ataaaatgga agttattccg
    11461 ccaaagatta ttgaagttgg taagattgct actgtaagaa cttctccaag cgtaaagaag
    11521 aatgcagcac tcggcttctt actcggtttc cttgcatgtg cggcaatcgt ggttgtatac
    11581 gctgtaatgg atgatactat taagacagag gaagatattg agaaatacct gggagtttct
    11641 gttcttgcca aggttcctga cagaaaagat tttataaatt cgaagaacag gaaatcaaag
    11701 aacaagaata agaagcacca ttaaactgat ggaggaatct gagcaatgaa gaaactcaat
    11761 ttaacagata aaagaaatcc ggattatttt tacagtgagg cgatcaaaac tctgcgtacc
    11821 aatattcagc tgtcgggtca gagcataaag acaattctgg ttacaagctg ttatccaaac
    11881 gaaggtaaga gtgatatagt actcagtctt gcccaggagc ttgggagcat tggcaagaag
    11941 gttctccttc tggatgcgga tatcaggaag actgcatatg ccggacgact gggtgtggaa
    12001 gaggaagtga aaggtctctc acagcttctg agcggtcagg tgggattaca ggaggttatt
    12061 tattctacaa actttccgaa tatggatatc atattcggag gtccaaatgc accgaacccg
    12121 tcaggacttc tcagtgagaa tatctttaag gtattcttaa aggaaatcag agaatattat
    12181 aattatatcc tgatcgatac accgcctatc ggaacagtaa ttgatgcggc agtgatcgga
    12241 agatgctgcg atggtgcagt ctttctgatt gaaccgggaa atgtcaggta ccgggacgca
    12301 cagaaagcat ttaaacagct ggaaagaagc ggatgccgta ttcttggagc tgttatgaac
    12361 aagatagata ccagtgatga caaatattat tcttcctatt ataagcatta tggagagtat
    12421 tatcgcagaa gcgacgaaga agcccagatt aaataatacg ggcagttatc tggcagtaag
    12481 acagaaacca tgatcaatga taaaggagaa tttggaaaag tgagcaaaat aaggaataaa
    12541 aaaataaaaa agacgaatat cttactgggc tttatctgca ttctgctggc agttctcctg
    12601 ggctttattc ttgtgaccag ttataaatcg gaacagaagg aatccagaca cctgtcagaa
    12661 attgcggacg accagcagag tgggattgaa gattacgaag cagtaaaaga acgtgcaaaa
    12721 gagctggagg aggtatcttc cgaagaagca gaagatacag aagacattaa atcaaagaac
    12781 acagaagaca caaaagacac agaaaaaacg gatacagctg acacagaaga tgcagataag
    12841 acagaagata caaaagataa gacgacagct gcttcaggta ttgtgtgctg gggagatgat
    12901 ctgatcaatg gggaagaatc caatacatat tcctatatga cagtacttca gaagcttctg
    12961 accgataatg gctataatat gacagtagtc aataaaacgc ttcagggagg cggcactctc
    13021 tccatgatga agatggctgg tgtaagtgat gaaactattc agagctatat taccagacat
    13081 cagcaggctg caaacggtgc ccagttgaat gtaacagaga ccggaatacg tgatctgaca
    13141 gaagaacaga ctacaagaaa tgatatggat tgtattccag ttattttcat gggatattat
    13201 ggcggctgga atcatgatcc tgcagaactg gcagaccagc aggaacagat tttaaataca
    13261 tttcagaata aagatcagtt tattgtagta ggaacacgcc ctatggacgg aagcgtaacc
    13321 tctgaagcac ttgatcaggt gctcagccag aaatggggag aacactatat cagtcttgca
    13381 gatgtgactg cacagccgtc ttctacctat gaggctcagc aggcaatggc agaggcaata
    13441 ttacagaaac tgcaggagct taattatatc agcaaaaact gatgagataa ctgaaataca
    13501 gagattgaag gaatgaaaat gaaaaaaacc acaagaaaag ccatcagaca gcgtaagatt
    13561 tgtatgcttt tgattattat cctgattata gttctttcca ttcttggcgg cggttattat
    13621 ctgttaagcc agaaaaatgc tgcatcgtca aaagccggtg gtcagaacag tgacagccgg
    13681 aatcagacgg atttgaacca aaacagtgat accgtggaat ataaaggtga gatatacaaa
    13741 tataacaatc atctcagcaa ttatttattc ctgggaattg acacaaggga agctgtagat
    13801 acttatcagt cacaggcaga tgccgggcag gcagatgcga ttttcctggt gtccatggat
    13861 cgtgcaacag agaagataaa ggttctgttt attccacgag attccatgac caggatagaa
    13921 gtatttaatc catatggtca gagccttgga gagacaacag atcatctgaa tatacagtat
    13981 gctttcgggg acggaaaaga gaaaagctgt gaactgatga aaacagcagt ttccgatatg
    14041 ctggacggac ttccgatcca ggggtattgt tccatgaaca tggatggcat ttcagtgatt
    14101 acagatttcg taggtggaat acagctcaca atccctgatg acagtctcgc agatgtgaat
    14161 tcggaatata aaaaaggtgc tgttgtggat atcacagggg aaactgcaga acagtttgta
    14221 agatatcgtg acattgacaa aacccagagt gctctggtcc gtcaggagag acagaaaacc
    14281 tttcttcagg cactggtgca gaaggctcag gaaaaagcag gagaggacgc cggatttgtg
    14341 acaggtcttt atgacagtgt taagagctat acagttacaa atatgggaaa tgatatattt
    14401 gccaaattgc tggctgcatc acagaatgga atcactgata cagagacagt accgggtgag
    14461 ggaacacatg gggagaactt tgatgagtat catatcgatg aagatgcatt gtcggatttg
    14521 attatttcca tgttttatga gaaaatttga ttgtattagt catagtatga tgaatacaga
    14581 atacactgta ctgtgataaa taattatcac tgttattatt cagagatagt ccgtgcagac
    14641 attctgtacg gttatctata taaaaattaa tccaaaagga gaggtgcatt atgaaaatcg
    14701 cgaagaaatt tatgacactt gctttagtag ctgcccttgg agtaggatgt gctactgtag
    14761 gtgtcggtgc agcgccaagc aacacgagtg atttggccgc ggtagcgcca agtaagacaa
    14821 gcgaaattac tgtggattct aaacagtctt caatttatga agttgttaag gaacttgaga
    14881 aaacacaggg ctttgctgat ctgcaggcaa aatataaaga tctggcagat gcttttaaaa
    14941 aaatcaatga tggtacaatg aagatggaag atttcataaa agtattgaaa tccctttctg
    15001 tagatgaagc agacaaggca gaccttgaag atgccattgc taaactcgaa ggaaaaatga
    15061 ttgttacatt tgtctatgaa ttcaacgtac tgaagccgga agaagcaaag cctaatgatg
    15121 acggaacata tgaagtaaag cttaatgttc cgagtcttac agacactatg gaaggtattc
    15181 agctttgggt ctacagcaaa gacggcacaa agaagtttgt agtcattgat ccggttaata
    15241 ttgacaaaga gggcaaaact cttactgtta ccttaaacga tggagatttc ttcatggtaa
    15301 tcgcagacgc aaaataatca ataattcgga atcagaacgc agtcgccaga gatggcggct
    15361 gtttttttgt agaaatatgt tgcatttttt tattgatgta gttataataa aatacggcgt
//
