LOCUS       c00290_gnlPro..         3451 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EIKHKFCI_290.
ACCESSION   c00290_gnlPro..
VERSION     c00290_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 06:49:52
            Original ID  :: gnl|Prokka|EIKHKFCI_290
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3451
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..591)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.4;
                     E-value: 8.8e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg290_1"
                     /sec_met_domain="Radical_SAM (E-value: 9.9e-33, bitscore:
                     101.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFNLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYH"
     protocluster    1..3451
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2821]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2821
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3451
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3451
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(593..2821)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg290_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1169.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWNICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIRALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gtgataagga agtatttcca cccgttgtat ctgctggtat ttgccaagac tttcacccag
       61 caggcgggtg tcttcttccg aatcgctgta accggggacc agtacatagc gcagccaaaa
      121 ggggtgttca tgttcttcga gccaggcagc ggtgcggagt gtctgttcgt tgcttcgtcc
      181 cgtcagcgta cggtggcgtt cgggattgaa ttgcttgatg tccagcagca cgaggtcagt
      241 caggttaaag agctcttcca ccttttcatt ccagatgcct ccgttactgt ccagacagat
      301 atggatgccg ttcgccttca gctccttgaa aagaggaatc agcgcttctg cttgcagggt
      361 aggctcaccg ccggagaaag taatacctcc tttcttcccg aagaatgcct tttggctgat
      421 agccatttgc agaatctctt ccggagtggt gggagtgcct ccttttacat caatcgtatc
      481 cggattggca caatacagac aacggaacgg acatccttgc aggaaaacga cgagccggag
      541 acccggcccg tcgaatgttc ccatactttc gtaggagtgt acatttatca tattacattc
      601 tttcgtggaa tgaacggctg atgacttcca actgatgctc gcggctcaac ttcacgaagt
      661 tcacggcata gccggaaacg cggatggtga gttgcggata cttttcagga tgttccatgg
      721 catcttccag catttcgcgg ttcagtacgt ttacgttcaa atggtgggca cctttggtga
      781 agtagccgtc catcatggtt accaggttct ctacacgttc ttccggtgtc gggcccagtg
      841 atttgggaac aatggagaac gtattgctga tgccgtcctg cgagtcgcgg tagcgcaatt
      901 tggctacgga gctcaaggaa gcgatggcac cgttcttgtc acgtccgtgc attgggttgg
      961 cacccggagc aaaggcaaca cctttggcgc gtccgtccgg agtggcaccg gtcttcttgc
     1021 cgtacatcac gttggaggtg atagtcagca aggaaagggt gggacgggca ttcttgtaga
     1081 caggcagctt tttcagttct tcgctgaaat agtaaacgag gtctactccc aggtggtcca
     1141 cacggtcgtc attattgccg aagcagggga atgagccttc ggtgtcgaaa ccttcggtca
     1201 ggccgatttc gttgcggcgt gcagtgactt tggcatattt gatggcggac agtgagtcta
     1261 tggcgatgga aaggccggca acaccgtatg ccagattgat gcgtgggtcg gtatcgatga
     1321 atgccatctg ggctttctca taataatact tatcatgcat gtagtggatg atattcatgg
     1381 cctcgttgta gacacgggct atttcagtca gtaccttctt gtagttggcc ataacttcct
     1441 caaacttcag tgtctcgccg gtcaatacgg gaataccttt caccatgaca gttccggtgt
     1501 tttcgcaacg gccaccgttg atggcaagca gcagggcctt ggcaaggttg gcacgtgcgc
     1561 cgaagaactg gatttgcttg ccgatggctt ggtaggatac acagcaggca atgccgtaat
     1621 cgtcgcagtt gcgcacttcg cgcatcaggt tgtcgttttc gtactggatg gagctggtat
     1681 ctacagaaac tttggcgcag aaatccttga agccttccgg cagttcggga ctccacaata
     1741 cggtcaagtt cggttccgga gaaggaccga ggttgtatag cgtctgcaaa aagcggaaag
     1801 aagtcttggt gactttcacg cgtccgtcgt tgaagcgtcc tccgatggct tcggtcaccc
     1861 aagtcgggtc gccggcaaag atgtcgttgt acgactgcat gcgcagatgg cgtaccatac
     1921 gtagtttgat gacgaattgg tctatcagtt cctgcgcaaa cgtctcgtca atctttccat
     1981 gagccaaatc gtattcgatg aaaatatcga ggaaagaaga tacgttaccc agtgacatgg
     2041 cagcaccgtc ctgctctttg atggcagcaa gatatgccat gtatacccat tgcacggctt
     2101 cttgggcaga tgtagccggg tggctgaggt caagcccgta atattcaccc attactttga
     2161 tgtctttcaa ggctctgatt tgttctgcca cttcttcgcg caggcggata cgtgcttcgg
     2221 tcatcgggcc tgtgaggtta cgcaagtcct cctgcttggc ttcgataagt cggtcgatgc
     2281 cgtagagtgc cagacgccgg tagtcaccga tgatacgtcc gcgggcatag ttgtcgggca
     2341 atccggtaag gaatcccagt gagcggaagg aacggatttc ttcggtatat acatcgaata
     2401 cgccgtcgtt atgtgttttg cggtaatggg tgaagatgtc ttttacttta tcgtccactt
     2461 ccactccgtt ttcacggcat gcttttgcta ccacgttgat gcctccgaaa ggtttgatgg
     2521 atcgtttcaa cagttcatca gtctgcaaac cgacaataag ttcattgtcc ttatcaatat
     2581 acccggcttt atgagaagtg atggtagata cggtggtatt gtccagtgaa cggacgccat
     2641 tgttgttcct ttcttcttcg agagccttca gacagatgtt ccagatacgt ttggtacgtt
     2701 cggtaggtcc ctgcaagaaa gatgcatccc cggtataggg tgcgatgttt ttactgacga
     2761 aatcactgac attgatttct ttattccaaa gcccattggt aaaggttttg ttcaattcca
     2821 taaaatttgt ttttgttagt aggttagata atgttatcct ttagagcgcg caaaaatact
     2881 gaataaattc cgtaatttta taagagagat tccccttttt atgttgaaca gcataaaata
     2941 actattatta ggtagtgaat gtccggggga atagagatgc taataccttc tttatgtgtg
     3001 gtttacgaac agaaacaaga atcggataat aatctgcatt atttacaaat aatgtaggtt
     3061 taagaacagt cgtattctga acctaattca gtcgtatagt gaatgccatt cagtcgtata
     3121 ctgaataggg ttcaatcgta tactgaatgt gactctttgt aagcatactg atattcagta
     3181 agttataaaa tattatcttg ccattgataa attttaacaa atagccaaaa tgccgttatg
     3241 ttcgttccgt agtgggtata aacaagaatt ttggtataat tgctctgttt ttgtgcagaa
     3301 aagtttgtag aataaaaaat gatttgtatc tttgcaccgc tttaaaagaa aagcacttct
     3361 gaaaaagaag ttttggagag gtggcagagt ggtcgattgc ggcggtcttg aaaaccgttg
     3421 tgctgcgagg cacccggggt tcgaatccct g
//
