LOCUS       c00133_gnlPro..        15601 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JKKHPPIG_133.
ACCESSION   c00133_gnlPro..
VERSION     c00133_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 03:33:05
            Original ID  :: gnl|Prokka|JKKHPPIG_133
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15601
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..720)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg133_1"
                     /sec_met_domain="MR_MLE_C (E-value: 6e-24, bitscore: 75.7,
                     seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKERFYKTEVVPRCLTFKRPAGTSRGVYTTRKLWEVRIRKEDEP
                     SAFGIGECAPLPDLSCDYGVDYEITLSKACLDLEQKGHVDTESLRHYPSILFGLEMAMR
                     HYEQGSWRHYDTPFSRGQAGIPINGLIWMGDYKYMLEQVEEKMKEGFRCVKLKIGAIDF
                     DRELSLLKHIRAHFSAKEIELRVDANGAFLPSEAMDKLKRLAEMDLHSIEQPIRAGQLE
                     EMARLAAESPLPIALDEE"
     protocluster    1..15601
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[743:5601]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      744..5601
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..15601
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15601
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(744..1568)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_2"
                     /sec_met_domain="ECH_1 (E-value: 7.7e-82, bitscore: 265.2,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METKREWTTIKEYEEILFDFYNGIARITINRERYRNAFTPTTTTE
                     MSDAFYICRERPDINVVVLTGAGDTAFCAGGDMNVKGRGGYVGKDGVPRLNVLDVQKQI
                     RSLPKPVIAAVNGFAIGGGHVLHVVCDLTIASENAIFGQTGPRVGSFDAGFGSSYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLDKLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATMLYYMTDEAQEGGKAFLEKRRPRFEDYPKFP"
     CDS             complement(1572..3260)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.1e-07, bitscore:
                     21.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYSDKKNILQLVALLIEHGVSNIVLCPGSRNSPIVHTLANHSFFN
                     CHSVTDERSAGFFAVGLALHGGKPAAVCCTSGTALLNIHPAVAEAFYQKVPLVVISADR
                     PAAWIGQMDGQTLPQPGVFGSLVKKSVQLPEIHTDQDEWYCNRLINEVLLELNHHGKGP
                     VHINVPISEPLFQFTTPELPKVRVITRYQGLNVYDRKYDDLIERLNKYSKRMMIAGQMS
                     LIYLFEKKICKLLYKHFTWLSEHIGNRIVPGLPIKNFDAILYATPEEEKEKLVPELVIT
                     YGGHIVSKRLKEFLRKHPPKEHWHVSLDGEVADLFGSLTTVIEMDPFEFLEKIAYMLEN
                     RQIEYPKAWEYKSRNLPEPEFAYSEMAAIGGLIRSLPAESALHLGNSSTVRYAQLYTVP
                     ETVEVCCNRGTSGIEGSLSTAIGYASASQKLNFIVIGDLSFFYDMNALWNGNLHNNLRI
                     LLLNNGGGEIFQALPGFKMNERTHRYVTASHQTSAEGWATERGFSYSAVHNAEELAAAM
                     SAFTQTEQPSEHPLFMEVFTDKAEDVRLLKEYYHQLKNSSQAQNI"
     CDS             complement(3275..4336)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg133_4"
                     /sec_met_domain="Chorismate_bind (E-value: 3.3e-54,
                     bitscore: 175.0, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LIKQGRSFAIWRIPGEDRLHFRMQSAGSPCLLYDIKDLNERSGFV
                     IAPFQVTAERPIVLIQPDCFEIPSESDWDFSREKNEFPSNEIEIPSESEEKERYAYHFS
                     LFTNPLLKGSQDKLVLSRSKTIWKDPSFSPGKAFFAAEERYIRSYVYLCHTPETGTWMG
                     GTPEILLSGEKGDWQTVALAGTQSLRDGKLPKSWDHKNWREQQLVASYIRRQLSTLGIT
                     PEEKGPYSARAGEVSHLKSDFFFSLPNPEKLGDVLQLLHPTPAVCGLPKEEAYHFIIEN
                     EGYDRSYYSGFIGWLDPKGKTDLYVNLRCMNILPQTFTLYAGGGLLAASQLEDEWQETE
                     DKLDTMRRIVGNL"
     CDS             complement(4369..5601)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_5"
                     /sec_met_domain="HBT (E-value: 4.1e-17, bitscore: 53.4,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDKKEITPRTHAALLKAQQMGVHVVLASGR
                     PTNGVQPLAEALELNHYGGFILSYNGGQIINAQTGELMFEKRIDPAMIPYLNRKAKENG
                     FAIFTYHKDYILTDSPENKHVQEEAELNKMRIIGVENFPEAVDFAPCKCILTSDDENNL
                     VGLENHWKKRLDGVLEAFRSEDYFLEVAPHFINKGNTLAVLMEMLNITTEEVVAIGDGV
                     ADVSMLQLAGTGVAMGNARDSVKACADFTTLPNNMEGVAVAIEKAILATIKPTEVPLDQ
                     LNARAKHALMGNLGIQYTYASEDRVEATMPVDERTRQPFGILHGGATLALAETVAGLGS
                     MILAKPDEMVVGMQVSGSHMSSAHEGDTVRAVGTIIHKGRSSHVWNVDIFTSTDKLVSS
                     VRVINSILKKK"
     CDS             complement(5730..6497)
                     /locus_tag="ctg133_6"
                     /transl_table=11
                     /translation="MNYKLLVLDLDGTLTNSKKEITPFTRETLLQAQEKGLHLVLASGR
                     PTYGIVPLAEELNIKQYGGFILSFNGGKVIDLMTDKVLFEQALPPDIVPVLYNRSKEAG
                     LAILSYNGKYILSEHPENKYVQYESFLTKMKIKATDDFLRDLHQPADKCLVVGEPEMLV
                     PLEEKLRQELGKRINVYRSEAFYLELVPKGIDKAASLSRLLERTRIKQEEVIAIGDGFN
                     DVSMIRFAGLGVAMANAQPPVKANAYTGIRLYQ"
     CDS             complement(6487..8409)
                     /locus_tag="ctg133_7"
                     /transl_table=11
                     /translation="MGISSPLVDLQHQYDLLQKEYEYEKETYRQQSERTGLRKRVAQGL
                     CWYPVTPGRSYYNSLNQLVIEIERTEDREIEHNFEYGRPVCFFTADISGKFKYLDFSAT
                     ISYVQEDRMVVVLPTPSALMDIQGKSNDLGVQLYFDETSYKTMFSALSDVMRAKNNQLS
                     HLRDVLLGQEKPGQRELFPIRFPWLNRSQEEAVNKVLGAKQVSIVHGPPGTGKTTTLVE
                     AIYETLHRENQVIVCAQSNTAVDCISEKLVDRGINVLRIGNPTRINDKMLSFTYERRFE
                     SHPDYPELWSIRKAVRDIQSNMRKKSREERDTIRNRLSRLKFRATELEVKIDTELFDEA
                     RVVACTLVGSANRVMMNRHFTTLFIDEAAQALEAACWIAIGKADRVILAGDHHQLPPTI
                     KCIEAEREGLGRTLMQKIAHAKPETVSLLKIQYRMHEDIMRFSSQWFYHNELESAPEVS
                     GRGILRLDTPIVWFDTSECDFTENTREETMSRVNRQEAELLVEQLRSYIQKISKERVLE
                     ENIDFGLISPYKAQVQYIRKLIKQDAFFKPLRRLITVHTVDGFQGQERDVILISLVRAN
                     EDGKIGFLNDLRRMNVAITRARMKLMILGDASTLTRHAFYKELYNYISQRGKIITINTK
                     QEHEL"
     CDS             8595..8966
                     /locus_tag="ctg133_8"
                     /transl_table=11
                     /translation="MNENDAFYNALIDVAAWDKENTLDRFMNSDSLFKKFLYKFITTDG
                     SYVKLRSSLVERDLENAKLYSHTLKGIAANLGLVNLFDVSNNILSLLREGNLEEALRYE
                     DALGKQYDALRDIVVEFCS"
     CDS             9101..10303
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SDH_alpha"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg133_9"
                     /sec_met_domain="SDH_alpha (E-value: 1.7e-60, bitscore:
                     195.8, seeds: 645, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LAAAKAAEVLGRKPEKIEVYLSANILKNAMGVGIPGTGMVGLPIA
                     IALGSIIGKSAYGLEVLKDLTPEGLKEGKEMVCKKCIGIDLKENVDKLYIEIISSAGSD
                     RSRVIICHEHTHIIYVEKNGEVLTDLRTANASGEEVCENKDLRLSFSMVYEFAMEMPLD
                     EIRFILETAELNKKAAQASMKGNYGHTVSKTVSGAFGRKFMGDSAYTHMLIMTSAACDA
                     RMDGAMIPVMSNSGSGNQGIAATLPVLSFAEDIQCSEEQLIRALMLSHLMVIYIKQSLG
                     RLSALCGCVVAATGASCAITYLMGGNKARISYAIKNMIGNITGMICDGAKPSCAMKVSS
                     GVSTAMLSALMAMEDKVVTSVEGIIDEDVDKSIANLTAIGSKGMEATDRLVLDIMTGKS
                     C"
     CDS             10391..11215
                     /locus_tag="ctg133_10"
                     /transl_table=11
                     /translation="LVFKFVAGMWGHSSAMIADAVHSLSDFVTDIIVLLFVNISSKPKD
                     ESHDYGHGKYETLATSIVGIVLLCVGFGLFWEGANKIYDFYFKGIPLESPGMIALVAAL
                     ISIVIKELLYHITVWVGRKQNSQVVIANAWHHRSDAFSSIGTALGIGGAILLGDNWRVL
                     DPLAAVIVSLFIVKVALQLVIPAINDLLEKSLPKEVEDEILAIISEVPEVKSPHNLRTR
                     RIGNNFAIEAHIRVDGQITVTRAHDLTKEIEQNLRDKFGQATHIALHVEPIK"
     CDS             complement(11246..11641)
                     /locus_tag="ctg133_11"
                     /transl_table=11
                     /translation="MNRGISGTSRSSVLHQFLHEKASDILCIFHGRESFLGGKRIFVQP
                     IQKLLAIHSLYLRLGEMDMGINKAGCNQFTGIVCHREIRVTGTHRLERPRLYNLSINNS
                     QSPILKIAVSLHMIQRGSDQIEHFSPI"
     CDS             12068..12568
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg133_12"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.7e-29, bitscore:
                     92.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGETGGIIAHCPTSNLFLGSGLFDMREANRYGVRTTLATDVGAGT
                     SFSMWRTMGEAYKVQQLNGYPMTALEAIYKCTLGSARALSLDDRIGSFLPGREADFIVV
                     DYAATSVQKLRMEYLRSRDKWTIENKLFGLQTLGDDRNMVCTYIMGKQVYGSDPCDHQE
                     ASM"
     CDS             complement(12533..13285)
                     /locus_tag="ctg133_13"
                     /transl_table=11
                     /translation="LLFSIVFFGQLALGIFADPIFLMTGKLHLPIPAVILAGPLYRFDG
                     LFMPILFISTLLLSGPAWCSQLCYFGAFDAWSARGKLERKRFPYHKQMRYSVLFLVMLG
                     AILLRIFGASGKIATAFGIAVGVIGLLVMLLFSRKRRKMVHCSSYCPIGALVSFLKYLS
                     PFRVRLTTDCCHCMACTNICRYDALHKEEIKKGKIGYTCTYCGDCLSACKHGAIEYHFP
                     GLRPATAERLWIVVTVVLHTCFLMIARI"
     CDS             complement(13546..14535)
                     /locus_tag="ctg133_14"
                     /transl_table=11
                     /translation="MNGFQGSTIEDFANAMGTTVQYTNYRLLFPNDDVQPKVSGNYALQ
                     VYNEDDPSQIVFTACFSIFEPMVSVAATVSGNTDIDTNQSHQQVSFAINNKNFPITYPQ
                     TDLKIWVYQNNRRDNAVTGLQPMTILENQISYTNNQNLIFPAGNEYRRMEFLSNKYNGM
                     HVESISFHNPYYHVELMTDYRRDQLSYQYDQDQDGRFFIRCSNCNDPDTEADYYIVHFT
                     LAMDLIPDGNVYLNGELYNNVLDEKSKMGYNPETHQYEKSVLLKQGSYNYQYLFVPNGQ
                     TVGQTGPIEGNFFQTENEYSIYVYYCPMGARYDRLIGVTSIRNTMPVL"
     CDS             14581..14772
                     /locus_tag="ctg133_15"
                     /transl_table=11
                     /translation="MNQIIGVSPEARIKSIEINFDTVFTAQFNEWIGEHLAGNLHLEGL
                     YLLIANVGEVSHLSVQRY"
ORIGIN
        1 ttcctcgtcg agagcgatgg gaaggggtga ctctgccgca aggcgtgcca tctcttccaa
       61 ctgcccggca cggataggct gctcgatgga atgcaagtcc atctcggcga gccgcttcag
      121 tttgtccatc gcctccgagg ggaggaaagc gccgttggca tctacccgaa gctctatttc
      181 cttggcagag aaatgcgcac ggatgtgttt caataaagag agttcccggt cgaaatcgat
      241 agcgccgatc ttcagcttga cgcagcgaaa gccctccttc atcttttcct ctacctgttc
      301 cagcatgtac ttataatcgc ccatccagat cagtccgtta atggggatac ccgcctgccc
      361 tcgcgagaag ggcgtgtcgt aatgccgcca actaccctgt tcataatgac gcatggccat
      421 ttccaatccg aaaaggatgg atgggtaatg gcgcaaagat tcggtgtcaa catgcccctt
      481 ctgttccaga tcgaggcaag ccttggataa cgtgatctcg taatccactc cataatcgca
      541 actgagatcc ggcagggggg cacattcacc gatgccaaaa gccgagggtt cgtcttcctt
      601 gcggattcgg acctcccaca atttccgggt cgtgtaaaca ccccgtgagg ttcctgccgg
      661 tcgcttgaaa gtcaagcacc ggggaaccac ctctgtctta taaaacctct ccttcaccat
      721 tcattctcaa ttttcaattc cttttacggg aacttcggat aatcctcgaa gcgtggacgg
      781 cgtttctcca agaatgcctt gccgccttcc tgcgcctcgt ccgtcatata gtaaagcatg
      841 gtagcgtctc cggccaactc ctgtataccg gcctgtccgt ccaactcggc gtttaagccc
      901 gccttgatca tgcgcaaagc caacgggcta agcaacatca tctcctcggc ccattgaacg
      961 tactcgtcct ctaatttatc caaggggact accttattta ccaaacccat atccaaagcc
     1021 tcctgcgcat tgtacttgcg gcataggaac cagatctcac gggccttttt ctgtccgacc
     1081 acacgggcga ggtaagaaga gccgaacccg gcgtcgaagc tacccacacg aggaccagtc
     1141 tgtccgaaga tagcgttctc ggaagcgatc gtaaggtcgc aaaccacgtg caatacgtgt
     1201 ccgccgccga tagcgaaacc attcacggcc gcgatgacgg gtttcgggag agagcggatc
     1261 tgcttttgta cgtccaatac gttcaagcga ggtacgccgt ctttacccac gtagccgcca
     1321 cgtcctttca cgttcatatc gcctcccgcg caaaaggccg tatctccggc tcctgtcaga
     1381 acgactacgt taatgtccgg gcgttcccgg cagatgtaaa aggcgtcgct catctccgtg
     1441 gtcgtagtcg gggtaaaagc gttgcgatac cgttcccggt tgatcgtaat gcgggcgatg
     1501 ccgttgtaga aatcaaataa gatctcctca tattccttga tggttgtcca ttctcttttc
     1561 gtttccatat attatatgtt ttgtgcttgg gatgaatttt ttagttgatg ataatattcc
     1621 ttgagcaatc gtacgtcttc cgctttgtcc gtgaaaacct ccatgaatag gggatgttcg
     1681 ctcggttgct ccgtttgggt aaaggcgctc atcgccgctg ccaactcctc ggcattatgg
     1741 acagcggagt aggagaatcc cctttccgtg gcccatccct ccgctgaggt ctggtgggag
     1801 gcggtaacgt aacggtgcgt acgctcgttc atcttgaatc ccggcaaggc ttggaagatc
     1861 tcgccacctc cgttgttcaa gagcaggatg cgaaggttat tgtgcaagtt accgttccac
     1921 aacgcattca tatcatagaa gaaactaaga tcgcctataa cgataaagtt tagtttttgc
     1981 gaggccgagg cgtatccgat ggccgtagac aaggagcctt cgatcccgct cgtacctcgg
     2041 ttacaacaaa cctcaaccgt ctcgggtacg gtgtacaatt gcgcgtaacg tacggtggag
     2101 ctattcccca gatgcaaggc cgattcagcc ggaagcgagc gtatcaaacc accgatcgcc
     2161 gccatttcgg agtaagcgaa ctccggctcc ggcaggttcc gggatttgta ttcccaagcc
     2221 ttcggatatt caatctgcct attctccagc atataagcga ttttctccag aaactcgaaa
     2281 gggtccatct cgatcaccgt ggtcaggcta ccgaataaat ccgctacctc tccatccaat
     2341 gagacatgcc aatgctcctt aggcgggtgt ttccgcaaaa actctttcag ccgcttggag
     2401 acaatatggc caccgtaggt gattacgagc tcgggaacta gcttctcttt ttcctcctcc
     2461 ggggtagcat acaggatagc gtcgaaattc ttgatcggta atcccggtac gatacggttg
     2521 ccgatgtgct cggagagcca agtgaaatgc ttgtatagta acttgcatat tttcttctcg
     2581 aagagataga tcaagctcat ttgtcccgct atcatcatcc gcttgctata tttgttcaag
     2641 cgctcgatca ggtcatcata tttccggtcg tacacgttca agccttgata acgggtgatt
     2701 acccgtacct tcggcaattc gggtgtcgtg aactgaaata aaggctcgga gatcggaacg
     2761 ttgatatgca ccggtccttt gccgtgatgg ttcagttcca acaacacctc gttgatcaat
     2821 cgattgcaat accattcatc ttgatccgta tgtatttccg gtagctgcac cgacttcttt
     2881 accagcgagc cgaacacccc gggttgcggc aaggtctgtc cgtccatttg cccgatccat
     2941 gcggcggggc ggtcggcgga tatcacgaca agcggaactt tctgatagaa agcctccgct
     3001 accgccggat gtatattcag caaggccgtg cccgaggtac agcaaacagc cgccggctta
     3061 cctccgtgca acgctagccc tacggcaaag aatcccgcgc ttcgttcatc cgtcacggaa
     3121 tggcaattga agaacgaatg gtttgccaat gtgtgcacaa tcggagagtt ccggcttccc
     3181 ggacatagca cgatattact tacgccgtgc tctatcagca aggctaccaa ttgaaggatg
     3241 ttctttttat cgctatacat tatatgatat aaaattataa attacccact attctccgca
     3301 ttgtatctaa tttatcctct gtctcctgcc actcatcctc tagctgcgag gccgccaaca
     3361 gaccgcctcc ggcatacagc gtaaaggttt gggggaggat attcatgcaa cggaggttta
     3421 cgtacagatc tgttttgcct ttgggatcca gccaaccgat gaaaccggag taatagctac
     3481 gatcgtaccc ctcattttcg atgatgaaat gataagcctc ctccttggga agaccgcaga
     3541 cggctggagt agggtgtaat agttgaagga catcacccag cttctcggga ttcggcaagg
     3601 agaagaagaa atcacttttc aaatgagaaa cctctccggc ccgggctgaa taaggcccct
     3661 tctcctcggg agtaataccc aaggtggaca gctggcggcg tatataggag gcgactaatt
     3721 gctgttctct ccaattctta tggtcccagc ttttcggtaa tttcccgtct cgtaaggatt
     3781 gggttccggc caaagctacc gtttgccagt cacctttctc gccggataac aagatttccg
     3841 gtgtcccacc catccaagtt ccggtttccg gggtatggca aaggtagaca taggagcgga
     3901 tatatcgttc ttccgcggcg aagaatgcct tgccgggtga gaaagaggga tctttccaga
     3961 tcgttttgct acgggatagg actagtttat cttggcttcc ttttagcaaa gggttggtaa
     4021 acagggagaa atggtaagcg tatcgttcct tctcctcgga ttcggacggg atttcgattt
     4081 cattcgaagg gaattcgttt ttctctcgag agaaatccca atcggattcg gaggggattt
     4141 caaagcaatc cggttggatt aaaacgatag gtcgttccgc tgtcacctga aacggagcga
     4201 ttacaaaacc gctccgttca tttaagtctt ttatgtcgta taataggcaa ggagagccgg
     4261 cactttgcat cctgaaatgg agcctatcct caccgggaat acgccaaata gcgaaactcc
     4321 ggccttgctt gatcaacgtg tctattattt cgtaagaatc tcttggaatc atttcttctt
     4381 tagtatacta ttgattaccc gtacagagga aaccagtttg tcggtagagg tgaatatgtc
     4441 tacgttccac acatgggatg aacggccttt gtggataatc gtcccgacgg cacgtaccgt
     4501 gtctccctcg tgggcggacg acatatggct gccgctaact tgcatgccga ctaccatctc
     4561 gtccggtttc gccagaatca tggaacctag tccggccacg gtctctgcca gagccagtgt
     4621 agcgcctccg tgcaagatac cgaaaggctg gcgggtccgt tcgtctacag gcatggtcgc
     4681 ttccacacga tcctctgacg cataggtata ttgaatgcct agattaccca ttaaagcatg
     4741 tttggcccgt gcgtttagtt gatctaacgg aacctctgtg ggtttgatcg tggcaaggat
     4801 cgctttctcg atcgctaccg ccacaccttc catattattg ggtaaggtcg tgaaatcggc
     4861 acaagccttt acggaatcgc gtgcgttacc catcgccacg ccggtaccgg ctaattgaag
     4921 catggatacg tcggccacgc catcaccgat agcgacaacc tcttcggtcg tgatattcag
     4981 catctccatc aataccgcca acgtatttcc cttattgatg aaatgaggag ctacctccaa
     5041 gaaatagtcc tcggagcgga aagcttccaa taccccgtcc agacgtttct tccaatgatt
     5101 ttcgagaccc actagattgt tctcgtcatc gctggtaaga atacacttac agggtgcgaa
     5161 atccaccgct tccgggaaat tctccacacc gatgatgcgc atcttattga gttcggcctc
     5221 ctcttggaca tgcttgtttt ccgggctatc ggttaggata tagtccttgt gatatgtgaa
     5281 gatcgcgaaa ccgttttcct tcgccttccg gttcaaataa ggaatcatgg ccggatcgat
     5341 tcgtttctcg aacatcaatt ctccggtctg ggcattgatg atctgacctc cgttataaga
     5401 taatatgaaa ccaccgtaat gattcaattc aagggcctcc gccaagggtt gaaccccgtt
     5461 ggtgggtcgg ccggatgcca atacaacatg aactcccatc tgctgagcct ttaacaaggc
     5521 ggcatgggtc cggggagtta tctctttctt gtcgttaagc aacgtcccat ctacgtctaa
     5581 gactaataat ttgtacttca tggcgtctta tttttatggt ttagaataca aatatacata
     5641 aaatagttga tagttagaag acatttccat gaaaggaatt tacgtatatc ggcgcaagct
     5701 cacgcctata ctgacgtagc cctacggcac tactggtata ggcgtatgcc ggtataggca
     5761 ttggccttga cagggggctg ggcgttggcc atagcgactc ccaatccggc gaaacggatc
     5821 atggatacgt cattgaaacc gtcgccgata gcgatcactt cctcctgttt tatccgggtt
     5881 cgctctagta agcgggataa cgaggcggct ttgtcgatgc ctttggggac aagctccaga
     5941 tagaatgcct cggaacgata tacgttgata cgtttcccta attcttggcg gagcttctct
     6001 tccaacggta ccaacatctc cggttcgcct accaccaagc atttatcggc tggctgatgc
     6061 aaatcccgca gaaaatcgtc ggtagccttg atcttcattt tggttaagaa agattcgtat
     6121 tgtacgtatt tattttccgg atgctcgctt aagatatatt taccgttgta ggaaaggata
     6181 gccagtcccg cttctttgga gcggttgtat aaaacaggta cgatatcggg tggcaaagcc
     6241 tgttcgaaaa gtactttgtc tgtcatcaag tcgatcacct taccgccgtt gaaggaaagg
     6301 ataaaaccac catattgctt tatgttcagt tcctcggcta aaggcacgat accgtaggtg
     6361 ggccggccgg aggctaagac cagatgcaat cctttttcct gcgcttgcag aagggtttcc
     6421 ctcgtaaacg gggtgatctc ttttttactg tttgtcagtg taccgtctaa atccagtacc
     6481 aataatttat agttcatgct cttgtttcgt gttgatcgtg attattttac ctctttggga
     6541 gatataatta tagagttctt tataaaatgc gtgccgggta agggtggaag cgtctcccaa
     6601 gatcatcagt ttcatacggg cacgggtgat cgctacgttc atgcgccgca agtcgttcaa
     6661 gaagcctatc tttccatcct cgttggccct taccaaactg atcaagatga catcgcgttc
     6721 ttgcccttgg aaaccgtcaa cggtgtgtac ggtgatcagt ctgcgtaaag gcttgaagaa
     6781 agcgtcttgt ttgatcaatt tacggatgta ttgtacctgc gccttataag gggagatcaa
     6841 tccgaagtcg atattctctt ccagcactct ctccttggat attttctgta tataggagcg
     6901 taattgttcc acgagcaatt ccgcctcttg cctgttgaca cggctcatgg tctcttctct
     6961 cgtattttcc gtgaaatcgc actcggacgt atcgaaccat acgatgggag tgtccagtcg
     7021 caagattccc cgcccactta cctcgggagc ggattcgagt tcgttatgat agaaccattg
     7081 ggaggagaag cgcatgatat cctcgtgcat acgatattgg atcttcaaga gagagaccgt
     7141 ttccggtttc gcatgggcta tcttttgcat caaggtacgt cctaagcctt ctcgttccgc
     7201 ttctatacat ttaatagtag ggggcaattg gtgatgatcg cccgctagta tgacccggtc
     7261 cgccttgccg atcgctatcc agcaagcggc ttccaaggct tgggcggcct cgtcgataaa
     7321 taaggtcgtg aagtgccggt tcatcatgac ccggtttgcg gagccaacca aggtgcaagc
     7381 gacaacacga gcctcgtcga acagctctgt atcgatcttg acttccagtt cggtcgcccg
     7441 gaatttcaaa cgggagaggc ggtttcggat cgtatcccgt tcctcccggc ttttctttcg
     7501 catattggat tgtatatccc gtaccgcttt tcggatgctc cataactcgg gataatccgg
     7561 atgcgattcg aaacggcgtt catatgtgaa agagagcatc ttgtcattga tacgagtggg
     7621 gttcccgatg cgcaggacgt taatgcctct atctaccagt ttctccgaga tgcagtctac
     7681 ggcggtatta ctttgggcgc aaacgatcac ttgattctcc cggtgaaggg tctcgtaaat
     7741 ggcttccacc aaggtggttg ttttcccggt tccgggaggg ccgtgtacga tagatacttg
     7801 tttcgctccc aataccttgt ttacggcttc ttcctgcgag cggttcaacc aagggaagcg
     7861 gatcgggaat aattcacgct gtcccggttt ttcctgcccc agcaatacgt ccctaagatg
     7921 ggagagttgg ttgttttttg cccgcatcac gtccgagaga gcggaaaaca tcgttttata
     7981 gctggtctcg tcgaagtaga gttgcacgcc gagatcattg ctttttcctt gtatatccat
     8041 caaggcggaa ggagtgggga gaactaccac catcctgtcc tcttgcacgt agctgatcgt
     8101 ggcggagaaa tccagatact taaatttacc actgatatct gccgtaaaga agcaaaccgg
     8161 gcgcccgtac tcaaaattat gctcgatctc ccggtcttct gtcctttcga tctcgatcac
     8221 caattggttt aaggagttat aataactgcg tcccggagtg accggatacc aacagaggcc
     8281 ctgcgccact cgttttcgca aacccgtacg ctcggattgc tgtcggtatg tttccttctc
     8341 atattcatat tctttctgca ggagatcata ttgatgttgc aaatctacaa gcggcgatga
     8401 aattcccatt cctttttcaa ttctattaat cggataaata cagcccgggc gttctaggtc
     8461 ccgaagtagg cgacaaaagt actaaaatta tcgttccgta cccaagttgc ggtaagtaaa
     8521 gaaaaagaat accgctaacg ttggtagtgt ctataaaatt cctaattttg tacgaaaact
     8581 aatatcataa aaaaatgaat gagaacgatg ctttttataa cgcattaata gatgtagctg
     8641 cttgggataa agagaatacc ttagatcgtt ttatgaatag tgatagcctc ttcaaaaagt
     8701 ttctatataa atttattact acagacggtt cgtatgtgaa attacggagc tctttagtag
     8761 agcgggattt ggagaatgcc aagctatatt cgcatacctt gaaagggata gccgctaatt
     8821 taggtctggt aaatctattc gatgtatcga ataatatact atcgctgttg agagaaggga
     8881 atctggaaga agcgttgcgt tatgaagatg cattgggaaa acaatacgat gccttgcgag
     8941 atatcgttgt tgagttttgt tcttaatcta ttgtatatta actatctaat tctaaataaa
     9001 gactatcagt atggatacta tcataaaaac tcaaattata gatttgattc acagggaggt
     9061 aatccctgct atcggatgta cggaacctat tgccgtggct ttggcggccg caaaagcggc
     9121 ggaagtgctg gggcgtaagc cagagaagat agaggtttat ctgagcgcca atatcctgaa
     9181 gaatgccatg ggagtaggaa tcccggggac tggcatggtg gggttgccta tcgctatcgc
     9241 tttgggatcg atcatcggta agtccgctta tgggctagag gtgctgaaag acttgactcc
     9301 ggagggattg aaagaaggta aggaaatggt ctgcaagaaa tgtatcggta tagacctgaa
     9361 agagaatgta gataaacttt atatcgaaat tataagttct gcagggagcg atcgttcaag
     9421 ggtgatcatt tgccatgagc atacccatat aatatatgta gagaaaaacg gggaggtatt
     9481 gactgattta cgtacggcaa acgcttctgg cgaggaagta tgtgagaata aggatttacg
     9541 tctttccttc tctatggtct atgagtttgc catggaaatg ccattggatg agattcgttt
     9601 tattttagaa acggccgagt tgaataagaa agctgcccaa gcttccatga aaggcaatta
     9661 cggacatacc gtaagcaaga cagtctcggg tgctttcggc cgtaagttca tgggagattc
     9721 cgcttatacg catatgttga tcatgacttc cgcagcttgc gacgctcgca tggacggcgc
     9781 tatgatcccg gtgatgagta attcgggaag cggtaatcaa ggaatcgccg ctacgcttcc
     9841 ggttctttct ttcgccgagg atatccaatg ctcggaagag caactgatcc gtgcgttgat
     9901 gttaagccat ttgatggtga tctatatcaa gcaaagtctg ggacgcttgt ccgccctttg
     9961 cggttgtgtg gttgccgcta cgggagcgag ttgcgctatc acctatttga tgggaggaaa
    10021 taaggcgcgg atttcttatg cgatcaagaa tatgatcggt aatattaccg gaatgatctg
    10081 tgacggtgcc aagccgagtt gcgcgatgaa agtatctagt ggcgtatcta cggcgatgct
    10141 ttccgctttg atggccatgg aggataaggt ggtgacttcc gtggaaggta tcatcgatga
    10201 ggatgtggat aaatcgatcg cgaacttgac cgctatcggt tctaaaggaa tggaggcgac
    10261 ggatcgtctg gtgctggaca tcatgaccgg taaatcttgt taatgggatt ctatggagga
    10321 ggcaccaaag gcgaggaaaa aagagattat gcgagtgaca cttttagggt cattcgctaa
    10381 ttttgtttta ttggtattca agttcgtggc gggtatgtgg gggcatagta gcgctatgat
    10441 cgccgatgcg gtgcattcgt tatctgattt cgtaacggat ataatcgtac ttctttttgt
    10501 caatatatct tccaagccga aggacgagag ccatgattac gggcatggta aatacgagac
    10561 tttggcgact tcaattgtcg gtatcgtctt gctttgtgta ggttttggcc ttttctggga
    10621 gggagcgaac aagatttatg acttttattt caaggggatt ccgttggaaa gtccgggaat
    10681 gatcgcttta gttgccgctc ttatctctat cgtgatcaag gaattactgt atcatattac
    10741 ggtttgggta ggaaggaagc agaatagtca agtcgttatc gctaacgctt ggcatcatcg
    10801 ctcggatgcc ttttcctcga tcggtacggc tttaggtatc ggaggggcga ttttgttggg
    10861 ggataattgg cgtgtgctgg acccgttggc ggctgtgatt gtcagtttgt ttatcgtaaa
    10921 agtagccttg cagctggtga tcccggcgat taatgactta ttggagaaat ctttgccgaa
    10981 ggaggtggag gatgagatct tggcgatcat tagtgaggtt cccgaggtaa agagcccgca
    11041 taatctacgt acccgccgca tcggtaataa ttttgctatc gaggcgcata tacgggtgga
    11101 cggacagata accgtgactc gtgcccatga cttgacgaaa gagatcgaac agaatctgcg
    11161 tgataagttc ggtcaggcta cgcacatcgc tttgcacgtg gaaccaatta aataaacgaa
    11221 ttgttatttg accatgatga agaacttata taggggagaa gtgttctatt tgatcgcttc
    11281 cccgctggat catgtggagg cttaccgcta ttttgaggat gggggactgg ctgttgttga
    11341 tggaaagatt gtagaggcgg ggccgttcga ggcgatgcgt tcccgttacc cggatttccc
    11401 ggtgacagac tattccggta aattgattac acccggcttt attgattccc atatccattt
    11461 ctcccagtcg gagatacagg gaatgtatgg caagcaactt ctggattggc tggacgaata
    11521 tacgtttccc gccgaggaag ctttctcttc catggaatat gcacaagata tcgctcgctt
    11581 tttcgtgaag gaactggtga agaacggaac tacgacttgt gccgcttatg ccacggttca
    11641 tcctgcttcc gtaaccgcat tattttccgt ggctacggag tataatatgt gtatcttggc
    11701 tggaaaggtg atgatgaacc ggaacgctcc ggattatctg atggatacca cgcatcaggg
    11761 tgagttggac tgccgtagct taatcgagga ttgggacggg aaaggacgga atcactatgt
    11821 gatcacgccc cgtttcgcca taaccagtac gccggatcaa ttaaagtctg ccggtcgttt
    11881 acatgccgaa tatcccagta cctatataca gacacatctt tcggagaatc tgggagagat
    11941 tgaatctgtc ttatcgctct gtcccggtca tgcggattat ctggaggtct atgagcgtgc
    12001 cggattgttg accgatcgtt cgatattcgg gcattgtgtc catctaaccg atcgggaata
    12061 taagcggttg ggagaaacgg gaggcattat cgcccattgc ccgacctcga accttttcct
    12121 tggaagcgga ttattcgata tgagggaagc gaaccgttac ggcgtacgta ctaccttggc
    12181 gacagatgtc ggtgcgggta catctttctc tatgtggcgt acgatgggag aggcttataa
    12241 ggtacaacag ttgaacggtt atccgatgac agctttggag gctatctata aatgtacgct
    12301 aggctctgca cgggccttat cgttggacga ccgaatcgga agtttccttc ctggccgtga
    12361 agcggatttc atagtcgtgg attatgccgc tacctctgtc cagaaattgc gtatggagta
    12421 tcttcgttcg agagataaat ggacgatcga gaataagctg tttggcctac aaaccctcgg
    12481 cgatgaccgg aatatggtat gtacttatat aatgggcaag caagtatacg gctcagatcc
    12541 gtgcgatcat caagaagcaa gtatgtaata cgaccgttac gacgatccat agacgttcgg
    12601 cagtagccgg gcgtaatccc gggaaatgat attctatagc cccgtgcttg caagcggaaa
    12661 gacaatctcc gcaataggta caggtgtaac cgatcttccc cttcttgatc tcttctttat
    12721 gcagggcatc ataccggcaa atgtttgtgc aagccatgca atggcaacaa tccgtggtca
    12781 gtcgtacccg gaaaggagag aggtacttga ggaaagacac caaagctccg attgggcaat
    12841 agctactgca atgtaccatc ttccgtctct ttctggaaaa caatagcatg acaagtagcc
    12901 cgataactcc gaccgctatt ccaaaagcgg tggctatctt gccgctcgct ccgaatatcc
    12961 gtagcaagat ggctcccagc atgaccagaa ataatacgct gtatcgcatt tgcttgtggt
    13021 aggggaacct ttttcgttcc aattttcccc gggcactcca tgcgtcgaat gccccgaagt
    13081 aacaaagctg gctacaccaa gcgggaccgg atagtaataa ggtgctgata aaaagtatgg
    13141 gcatgaataa tccatcaaag cgataaagcg gtccggccaa gattaccgca gggatcggta
    13201 aatgcagttt cccggtcata aggaaaatgg gatcggcaaa aatccctaga gcaagttgcc
    13261 cgaaaaaaac gatcgagaat aacaaccacg ctcgttttct ccatttggga cgttcttgcc
    13321 gatcttgcat cttatagcag agccaaccac cgtatagcat ggcaagtatc acttgtatcc
    13381 atcctccata cggaaataac ctttcgccta tcaacagtgg atttttaggc atgacttgaa
    13441 cgatcgagag aagtcccccg actaataata cggtactgat aacaatacgt ctcgttttca
    13501 tgctcaatta ttttatgcgt aaaagtatgc cttttacgcg aataattata gcacgggcat
    13561 tgtatttctg atactagtta caccgattaa acgatcatac cgtgctccca ttggacaata
    13621 gtagacgtaa atgctatatt cgttctccgt ctggaagaaa ttaccctcga tagggccggt
    13681 ctgtcctacg gtctgtccgt tagggacaaa gaggtactga tagttgtaac tgccttgttt
    13741 cagtaagacg gatttctcgt attgatgcgt ctccggatta tagcccatct tacttttctc
    13801 gtccaataca ttattatata attcaccgtt caaatacacg tttccatcgg gaattaagtc
    13861 catggcgagg gtaaagtgaa cgatgtaata gtccgcttcc gtatccgggt cgttacaatt
    13921 actgcaacgg atgaaaaaac ggccgtcttg gtcttgatcg tattgatacg ataattggtc
    13981 acgccgatag tccgtcatca actctacatg gtaatatgga ttatggaacg agatactttc
    14041 tacgtgcatg ccattgtatt tgttgctcaa aaattccata cggcgatatt cgtttcctgc
    14101 gggaaagatc aagttctgat tgttcgtata agagatctgg ttttctaaaa tagtcatagg
    14161 ctgaagacct gttacggcat tatccctgcg gttattctga taaacccaga ttttcaagtc
    14221 ggtctgagga taggtgatgg ggaaattctt gttattgatc gcaaagctga cttgttggtg
    14281 tgactgattg gtatcgatat ccgtatttcc gctcaccgta gcggcgacac taaccatcgg
    14341 ctcgaaaata gaaaagcaag cggtaaatac gatctgggag ggatcatcct cgttatatac
    14401 ttgcaaggca taattccccg agaccttcgg ttgaacgtca tcgtttggga aaagtaatcg
    14461 gtagttggtg tattgcaccg tagtacccat cgcattggcg aaatcctcga tcgtgctccc
    14521 ttggaaacca ttcatatact caatcgggga aagattcgat tgcgtccaat cggcgttgca
    14581 atgaaccaga ttataggcgt atcgcccgaa gcccggatta aaagcatcga aattaacttc
    14641 gatacggttt tcaccgccca gttcaatgaa tggatcggag aacatctcgc cggcaacctt
    14701 cacttggagg gtctttattt gcttatcgct aacgttggtg aagtaagtca cttgagcgtt
    14761 caacggtact aaaaccgtga taagcagcag aaataggatt ttaaaaactt tcattttctt
    14821 attactttct ttaacgacgc aaaattatgg aattatttct ctatttattt gcctttccct
    14881 gctctaaagc atgaaaaaat gtttactttt gcaggaaaat tacatataaa caattttagt
    14941 tgtatcatgg caaatgtgat taagattaaa aagggtttag acattaatct gaagggcaaa
    15001 gcttcggatg tactgttaaa cggcggcaag agcgagtctt acgcgatcgt ccccgattat
    15061 tataacggta ttctccccaa agttgttgcg aaagtaggag aaaaagtcaa agccggttct
    15121 gtactgatga ttgacaagaa ccgccccgag atcaagtttg tttcgccggt cagtggcgag
    15181 gtaactgccg taaaccgagg agagaagcgt aaggtgctaa gcatcgttgt gaagccggat
    15241 gcgcagatcg agtatgagga gttcggtaag aagaacgtag catccctaca gggagcagag
    15301 gtgaaagagg tacttctgaa cgctggtatg tggccgttta tcaagcaacg cccatacgat
    15361 atcgttgccg ctccattgga tactccgcgt gatatcttcg tttcggcttt ttactccgct
    15421 ccgttggctc caaacttcga ttttatcgta aaaggtcagg aggctgattt ccagactgga
    15481 ttaaacgccc tagctaaatt aacgaatgga aaagtgtatg tgggtgtaag aagtggttct
    15541 gtcgtgagcg gaatgaaagg tgtggagatc gtggaagtag aaggtccgca tcccgcagcg
    15601 a
//
