LOCUS       c00215_gnlPro..         6278 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ILJGGLAL_215.
ACCESSION   c00215_gnlPro..
VERSION     c00215_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 04:13:17
            Original ID  :: gnl|Prokka|ILJGGLAL_215
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6278
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6278
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1332:5894]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1333..5894
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6278
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6278
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             161..1159
                     /locus_tag="ctg215_1"
                     /transl_table=11
                     /translation="MPLKLTTYYRGKDIPDLPGTNTFHSKELFQIYESTPGYTPLLIVA
                     TEEGRPVARLLAAIRKTKKWLPSSLVKQCVVYGAGEFLDTSLPASKEKEEEIFGEMLEH
                     LTQEASRTCILIEFRNLDNSMFGYRFFRKNDFFPVNWLRVRNSLHSTQKAEDRFSPSRI
                     RQIRKGLKNGAKVVEAHTVEEIKEFSRMLHKVYSSRIRRYFPANDFFRHMNSMLIRGKQ
                     AKIFIVKYKEKIIGGSVCIYSGENAFIWFSGGMRKTYALQSPGILAVWKALEDAHERGF
                     RHMEFMDVGLPFRKHGYRDFVLRFGGKQSSTRRWFRISWPWLNNLLVKFYV"
     CDS             1333..1737
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_2"
                     /sec_met_domain="Glyoxalase_4 (E-value: 7.4e-25, bitscore:
                     76.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             1768..3321
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_3"
                     /sec_met_domain="CT_Btheta (E-value: 2.3e-291, bitscore:
                     955.2, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERIAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYLFAQDFTVTAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGLALIRTLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPN
                     KPYDMYEVISAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKFLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             3353..4273
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_4"
                     /sec_met_domain="OAD_gamma (E-value: 1.9e-15, bitscore:
                     46.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLLLNEVLIDNQNNFQD
                     DYGLHSAWIEIFNKSYGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGRKLLDQVVIPAGTLGPNQSYARISDGAADW
                     EVKGGSKDKYVTPSTNNKTLDSNAKMEKFEEHDSVGIGMSISAMSVVFCGLILLFIAFK
                     VVGKVAVNLSKRNAMKAKGIDKVEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRENPNRK"
     CDS             4298..4732
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.7; E-value:
                     4e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 4.6e-22, bitscore:
                     66.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKTA
                     PKPVVVRPMPNSPAAPTTPVVKPAAPSTGKSGVKSPLPGVILDIKVNVGDTVKRGQTII
                     ILEAMKMENNINADKDGKVTAINVNKGDSVLEGNDLVIIE"
     CDS             4734..5894
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_6"
                     /sec_met_domain="OAD_beta (E-value: 1e-154, bitscore:
                     503.5, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHIVMILVGLFFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKHERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFIIATASGVIFVKIFNLFLKKGNKINPLIGNAGVSAVP
                     DSARISQVVGLEYDPTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             6108..6278
                     /locus_tag="ctg215_7"
                     /transl_table=11
                     /translation="MKRIILLAFLCTTTLVMAQRINHVQEAIANYDYEAALTLIAKEKP
                     TIPLLLQKGKAQ"
ORIGIN
        1 tctgtactca caaaggacga caataataca aaaattcgct tttccaagcc tttttttcct
       61 caaatatatg caaaggataa aaagacaaat tttaggctaa gccaacaaat tctctttttt
      121 attgttgaat gaagaataaa aagtgtaaat ttgtatttgg atgccactta aactaacaac
      181 atattatcgc ggaaaagaca ttcctgattt accgggaacc aatacttttc attccaagga
      241 gttgtttcag atctatgaat cgactccggg atataccccg ctactgatag tggcaacaga
      301 agaaggcaga cctgtagcac gcctgcttgc cgccatacgc aagaccaaaa agtggcttcc
      361 ctcctctctt gtcaaacaat gtgtggtata tggtgcaggt gaatttctgg acacttctct
      421 accggcaagt aaagagaagg aggaagaaat tttcggtgaa atgctggagc atctcacaca
      481 agaagcatca cgcacctgca ttcttattga attccgcaat ctggataatt ccatgttcgg
      541 ttaccgtttc ttccgaaaga acgacttttt tccggtcaac tggctgcgtg tccgtaactc
      601 tctgcatagt acgcagaaag cggaagaccg tttcagcccg tcacgtatcc gtcagatccg
      661 gaaagggctc aaaaacggag ccaaagtagt agaagcccat acggtggaag agattaagga
      721 attctcgcgg atgctgcaca aagtttattc ttcacgaata cggagatatt tccctgccaa
      781 tgactttttc cgccacatga acagcatgct tatcagagga aaacaggcaa aaatattcat
      841 cgtaaaatac aaggaaaaga ttatcggcgg ctccgtctgc atctattccg gcgaaaatgc
      901 ttttatctgg ttttcaggag gaatgcgaaa gacgtatgca ctgcagtcac ccggcatact
      961 ggcagtatgg aaagcacttg aagatgccca tgaacgggga ttccgtcaca tggagttcat
     1021 ggatgtagga cttcccttcc gcaagcatgg ttatcgtgat ttcgtactcc gtttcggagg
     1081 caaacaaagc agtacccgcc gctggttccg tatcagctgg ccatggctga ataatctttt
     1141 ggtcaagttt tatgtctaaa tgtaatctca agattacaaa acccgtcttt gtcacccatc
     1201 cggagcattt tttatgcttt attccccaat aattaaatgc agaaatattt ctttattaga
     1261 caagaaatgt ctttctttgt gctgttttat gccatataag cacataatta aaccatataa
     1321 aaaaatatag ttatgaagat ttcacacatt gagcatctgg gcattgctgt aaaaagcatc
     1381 gaagaagccc ttccttacta cgaaaacgta ttgggtctga aatgttacaa catcgaaaca
     1441 gtagaagatc aaaaagtaag aaccgctttt ttaaaggtag gtgaaacaaa aatcgaattg
     1501 ttggagccga cttgccccga aagcaccatt gctaaattta ttgaaaacaa aggtgcaggt
     1561 gttcatcacg tagcatttgc cgtagaagat ggcgtagcta atgctttggc agaagcagaa
     1621 agcaaagaaa tccgtctgat tgacaaggct ccccgcaaag gtgccgaagg tctgaacatc
     1681 gctttccttc acccgaaatc aacactgggt gtgctgacag aactctgcga acactaatca
     1741 taaactctaa aaaaacaaca agaactcatg agtaatcaac ttgaaaaaat taaagagctt
     1801 attgaacgcc gtgccgttgc acgcatcgga ggcggcgaaa aagcaattgc gaagcaacat
     1861 gaaaaaggga aatacacagc gcgtgagcgt atagccatgc tgttggatga aggtagcttc
     1921 gaagaaatgg acatgttcgt tgagcacaga tgcacgaact tcggcatgga gaaaaagcac
     1981 tatccgggtg atggtgtagt aaccggttgc ggtactatcg aaggtcgtct cgtatatctt
     2041 tttgcacagg actttaccgt tactgcaggt tcactgtcgg aaacaatgtc actgaagatc
     2101 tgtaagatta tggatcaggc aatgaagatg ggtgctcctt gtatcggtat caatgactcg
     2161 ggcggtgcac gtatccagga aggtattaac gctttggcag gttacgcaga aatcttccag
     2221 cgcaatatcc tcgcttcggg tgtcattccg cagatttccg gtatcttcgg tccttgtgcc
     2281 ggcggtgctg tttattctcc ggcactgaca gacttcacac tgatgatgga aggtacttct
     2341 tatatgttcc ttacaggtcc gaaagtagtg aaaactgtaa ccggtgaaga cgtaagccag
     2401 gaaaacctcg gcggagcaag cgttcactct accaaatcgg gtgttactca tttcacagcc
     2461 aaaacagaag aagaaggtct ggcattgatc cgcacacttc tgagctatat tcctcaaaac
     2521 aacctcgaag aagctccata cgtggattgc acagatccga tcgaccgtct ggaagattct
     2581 ctgaacgata ttatccccga cagccccaac aaaccgtatg atatgtacga ggttatcagc
     2641 gctatcgtgg acaacggcga attcctggaa atccagaaag actatgccaa gaatatcatt
     2701 atcggttttg cccgtttcaa cggtcagtct gtaggtattg tagccaatca gcctaaattc
     2761 cttgccggtg tactcgacag caacgcatct cgcaaaggcg cacgtttcgt tcgtttctgc
     2821 gatgctttca atatccctat cgtgtcgttg gtagacgtac cgggattcct tccgggaaca
     2881 ggccaggaat acaatggcgt tatccttcat ggtgccaaac tgctgtatgc ttacggtgaa
     2941 gctactgtac ctaaagtaac aatcacattg cgtaaatctt acggtggttc tcacatcgtg
     3001 atgagttgta aacaacttcg tggtgacatg aactatgctt ggccaacagc tgaaatcgct
     3061 gtaatgggtg gtgcaggtgc agtagaagta ttgtacgcgc gtgaagccaa agaccaggaa
     3121 aatccggctc agttccttgc cgaaaaagaa gcagaatata caaaactgtt tgccaaccct
     3181 tataatgcag ctaagtatgg ttacatcgat gatgtcatcg aaccgcgtaa cacacgtttc
     3241 cgcgtgatcc gtgctttgca gcagttgcag actaagaaac tgtctaaccc ggcgaagaaa
     3301 catggtaata ttccgttgta atccaacttt tcacattaaa acaagaacga ttatgaacaa
     3361 aaccaaaatc ggaatattcc tttctctgct gttgctgatt ggattgactt cctgcggaga
     3421 gcaaaagtcg aacaataagc tattgctgaa tgaagtcctg atagacaatc aaaacaactt
     3481 tcaggatgac tacggcttgc acagtgcatg gattgaaata ttcaacaaat catacggtag
     3541 cgcagactta gcagcttgtt tattgaaagt aagcagccag ccgggcgata cagtaaccta
     3601 ttttatcccg aaaggtgaca tacttacatt agtaaagcca cgccaacatg cgttattttg
     3661 ggcagacggc gaacctaatc gtggtacctt ccataccagc ttcaaactga accctgaaac
     3721 agccaattgg gtcggtcttt ttgattcagg cagaaagcta cttgatcaag ttgtcatacc
     3781 agcaggaact ttaggtccca accagtctta tgcacgcata agcgacggag cagcagactg
     3841 ggaagtaaaa ggcggaagca aagacaaata cgtaaccccg agcacgaaca acaaaaccct
     3901 tgacagtaat gccaagatgg aaaagttcga agaacatgac tcagtcggta tcggtatgtc
     3961 tatttcagcc atgagcgtag tattctgcgg attgattctt ctcttcattg ccttcaaggt
     4021 ggtaggtaaa gttgctgtca acctgagcaa acgcaatgcg atgaaggcta aaggcatcga
     4081 taaagtcgaa gcgaaagagc tttcacaggc tccgggtgaa gtttatgctg ctatttcaat
     4141 ggcattacac gaaatgcagg atgaggtaca tgacgtagaa gaaacggtgc tgaccatcac
     4201 tcgtgtgaaa cgcagttatt caccgtggag ttcgaagatt tacaccttgc gtgaaaatcc
     4261 taacagaaag taaatcattc cctaaaaaga taaaacgatg aaagaatata aatataaaat
     4321 caacggtaac tcatacaaag taaccatcgg agatattgaa gataatatcg ctcatgtaga
     4381 agtgaacggt acgcactaca aagtagaaat ggagaaacaa ccgaaaactg ctcccaaacc
     4441 ggtagtagta cgtcctatgc ctaactcacc ggctgctcct accacaccgg tagtaaaacc
     4501 ggctgctcct tctacaggca aatcaggcgt taagtccccg ctaccaggcg ttatccttga
     4561 catcaaagta aatgtaggtg atacagtgaa aagaggacag actatcatta tattggaagc
     4621 catgaagatg gaaaacaata tcaatgcaga caaagacggt aaagtgactg ctatcaatgt
     4681 caacaaggga gattctgttc ttgaaggtaa tgacctcgta attattgaat aatatgggag
     4741 attttattaa ctttttagga aataaccttg ccgacttctg gacctacacg ggatttgcca
     4801 acgcgacagt tggtcatatc gtgatgatcc tcgttggctt gttcttcatc tatttggctg
     4861 tagccaaaga gttcgagccg atgttgttaa ttcctatcgg attcggtatc ctgatcggta
     4921 atataccctt caatatggac gccggactaa aagtcggtat atatgaagaa ggttctgtat
     4981 tgaacatatt gtatcaggga gtaacgtccg gctggtatcc accgctcatc ttcttaggta
     5041 tcggtgccat gacagacttc tcggcgctga tctccaatcc gaagctgatg ctgattggtg
     5101 cagctgccca gtttggtatc ttcggtgcat acatgatcgc attggaaatg ggatttgacc
     5161 cgatgcaggc cggtgctatc ggtatcatcg gtggagcaga tggtccgacg gctatcttcc
     5221 tttcgtccaa gctggcacct aacttaatgg gagccattgc ggtatccgcc tactcctata
     5281 tggcgttagt tccggtgata cagccgccta tcatgcgtct gctcaccacc aaacacgaac
     5341 gcgttatccg tatgaaaccg ccacgtgccg tttctcatac agagaaagtg attttcccga
     5401 ttatcggtct gttgctgacc tgcttcctgg ttccttccgg tctgcctctg ttgggtatgc
     5461 tgttcttcgg taacctgttg aaagaaagtg gtgtaacccg tcgtctggct aacacagcca
     5521 gcggtccgct gattgacaca atcactatcc tgttaggttt gaccgtaggt gcttctactc
     5581 aggcttctga gttcctgaca ctcgactcca tcaagatctt cgccctcggt gctttgtcat
     5641 ttatcattgc taccgcatca ggtgtgatct tcgttaagat cttcaacctc ttcctcaaga
     5701 agggcaataa gatcaatccg ttgatcggta atgcaggtgt atctgccgtt cccgactctg
     5761 cacgtatctc acaagttgtc ggtctggaat atgatcctac caactacttg ctgatgcacg
     5821 ctatgggtcc gaacgtagca ggtgtaatcg gttcggctgt tgctgccggt atcctgttag
     5881 gattcttaat gtagggacaa ggtgcccctg tcaggtttcc attcggaatg ccgggacaaa
     5941 atattaggtg cggatattgc tgttttagat gcaatatccg cacttatcat tatgttatct
     6001 tctcttatca ttccgttaat ctattccaaa aaggctacga ataattcgta aatacgatat
     6061 tttttagcat cttgcgtctt tgttacgaca cactaaaata aatatttatg aaacggatta
     6121 ttctcctcgc cttcctttgt accaccaccc tggtgatggc gcagcgcatc aatcatgttc
     6181 aggaagcaat agcaaactac gattacgagg cagctctcac tctcattgcc aaagaaaagc
     6241 cgacaattcc cctactcttg caaaaaggta aagcacaa
//
