LOCUS       c00273_gnlPro..         6019 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FACJAAOP_273.
ACCESSION   c00273_gnlPro..
VERSION     c00273_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 08:08:16
            Original ID  :: gnl|Prokka|FACJAAOP_273
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6019
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6019
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1386:5943]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1387..5943
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6019
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6019
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             198..1202
                     /locus_tag="ctg273_1"
                     /transl_table=11
                     /translation="MVNYKDLGLVNTREMFAKAIKGGYAIPAFNFNNMEQMQAIIKAAV
                     ETKSPVILQVSKGARQYANATLLRYMAQGAVEYAKELGCAHPEIVLHLDHGDTFETCKS
                     CIDSGFSSVMIDGSHLPYEENVALTKKVVEYAHQFDVTVEGELGVLAGVEDEVSSDHHT
                     YTDPEEVIDFATRTGCDSLAISIGTSHGAYKFTPEQCHIDPKTGRMVPPPLAFEVLDAV
                     MEKLPGFPIVLHGSSSVPEEEVETINKYGGALKAAIGIPEEELRKAAKSAVCKINIDSD
                     SRLAMTAAVRKVFAEKPAEFDPRKYLGPARDNMEKLYKHKIINVLGSDNKLAQ"
     CDS             complement(1387..2547)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_2"
                     /sec_met_domain="OAD_beta (E-value: 3.4e-154, bitscore:
                     501.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLVFIYLAIAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKNERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFVIATASGVIFVKIFNIFLKKGNKINPLIGNAGVSAVP
                     DSARISQVIGLEYDSTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             complement(2549..2980)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPV
                     AKPVTVRPMPNAPAAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             complement(3005..3925)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_4"
                     /sec_met_domain="OAD_gamma (E-value: 3.3e-16, bitscore:
                     48.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILIDNQNNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGKKLLDQIVVPAGALGPNQSYARVSDGAAEW
                     EVKSGSGDKYVTPSTNNKTLDSNSKMEKFEEHDADGVGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             complement(3957..5510)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_5"
                     /sec_met_domain="CT_Btheta (E-value: 9.5e-291, bitscore:
                     953.0, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYVFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAQTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             complement(5539..5943)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg273_6"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.6e-24, bitscore:
                     75.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAEGKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 aaatattccg ttcctttgca gtctctaata taaataggta tgatggataa tttacaaaaa
       61 tataagccaa ctgataaatt ttgcagtctg atttaaacct ttttctacta tatttttctt
      121 tccgatgttc gttattcgat ggataatgct tacttttgcg tagaattttt attcactaac
      181 aataaacttt aaacaaaatg gtaaattaca aagatttagg attggtaaac acaagagaaa
      241 tgtttgctaa ggctatcaaa ggtggatatg ctatcccagc attcaacttc aacaatatgg
      301 aacaaatgca ggctatcatc aaggctgctg ttgaaactaa atctcctgtg attcttcagg
      361 tttctaaagg tgctcgtcag tatgctaacg ctactttgtt gcgttacatg gcacagggtg
      421 ctgtagaata tgctaaagaa ttaggctgcg cacatccgga aatcgttctt caccttgacc
      481 acggagatac attcgaaact tgcaaatctt gtatcgattc aggtttctct tcagtgatga
      541 ttgacggttc tcatcttcct tacgaagaaa acgtagcatt gactaagaaa gttgttgaat
      601 acgctcacca gtttgacgta actgtagaag gtgaacttgg cgtattggct ggtgtagaag
      661 atgaagtttc ttccgaccat cacacatata ctgaccctga agaagtaatc gacttcgcta
      721 ctcgcactgg ttgcgactct ttggctatct caatcggtac ttctcacggt gcttataaat
      781 ttactccgga acaatgccac atcgacccga agactggccg tatggttcct cctccattgg
      841 ctttcgaagt attggacgct gtaatggaaa aacttccggg attccctatc gttcttcacg
      901 gatcatcttc agttccggaa gaagaagtgg aaactatcaa caaatatggt ggtgcactga
      961 aagctgctat cggcattccg gaagaagaat tgcgtaaagc tgctaagtct gctgtttgca
     1021 aaatcaacat cgactctgac tctcgtctgg ctatgactgc tgctgtacgt aaggtattcg
     1081 ctgaaaaacc ggctgaattc gacccacgta agtatcttgg cccggctcgt gacaatatgg
     1141 aaaaactgta caagcacaaa atcatcaacg tgctcggttc tgacaacaaa ttggcacagt
     1201 aataatctcc ggattattcc ataaataaaa gaaagaggct gctccggttt gaacactggt
     1261 agcagccttt tttatgacgt ttaatcgttt cagaacggag agaagataac actatggctg
     1321 taataaagaa cacccgtgtc acaactgaat gtaacacggg tgtttttatc tattttataa
     1381 tgtttcttac atcaagaatc caagcaggat accggcagca acagctgaac cgatcactcc
     1441 ggcaacgttc ggacccatag cgtgcatcag caaatagttg gtcgaatcgt attccaaacc
     1501 aatcacttgc gagatacgtg cagagtcggg aacagcagaa acacctgcat taccgatcaa
     1561 cggattgatc ttattaccct tcttcaggaa gatattgaaa atcttaacga agatgacacc
     1621 agatgccgtt gcaataacaa atgacaatgc accgagggca aagatcttga tagagtcaag
     1681 cgtcaggaat tcggatgctt gtgtagaagc acctactgtc agacccaaca ggatagtgat
     1741 tgtatcaatc aacggaccac tggcagtatt agccaaacgg cgggttacac cactttcttt
     1801 caacaggtta ccgaagaaca gcatacccag caaaggcaga ccggaaggaa ccaggaaaca
     1861 agtcagcaac aaaccgatga tcgggaaaat caccttctcc gtgtgagaaa cggcacgtgg
     1921 cggtttcatg cggatgacac gttcgttctt ggtggtgagc agacgcatga taggcggctg
     1981 tatcaccgga accaacgcca tataggagta ggcggacacc gcaatggctc ccatcaggtt
     2041 aggtgctagc ttggaagaaa ggaagatggc cgtcgggccg tctgctccac caatgatacc
     2101 gatagcaccg gcttgcattg ggtcgaaacc catttccaat gcgatcatat atgcaccgaa
     2161 gataccgaac tgtgcagctg caccaatcag catcaatttt ggattagaga taagtgccga
     2221 gaagtccgtc atggcaccga tacccaaaaa gatgagcggc ggataccagc cggaggtcac
     2281 tccctgatac aatatattca atacagaacc ttcttcataa ataccgactt tcagcccggc
     2341 atccatatta aaaggtatat taccgatcaa tataccgaaa ccgataggaa tcagcagcat
     2401 cggttcgaac tctttggcta ttgccaaata gatgaatacc aagccaacga gaatcatgac
     2461 tacatgccct accgtagcat tggcaaagcc tgtgtatgtc cagaagtcgg ccaggttatt
     2521 tcctaaaaag tttataaagt ctcccatatt attcaataat tacgaggtca ttaccttcaa
     2581 gaacagagtc tcccttgttg acgttgatgg cagtaatctt accgtcctta tccgcattga
     2641 tattgttttc catcttcatg gcttccaata tgataatggt ctgtcctttc tttacagtat
     2701 cgcctacatt cactttgatg tcgaggatca cacccggcag cggagatttt actcctgatt
     2761 ttccggtaga cggagcggtc ggtttcacta cttgagtagg agcagcagga gcattaggca
     2821 tcgggcgtac tgtcacgggt ttcgcaacag gcttcggctg tttctccatt tctactttat
     2881 agtgtgtacc gttcacttct acatgagcga tgttgtcttc aatatctccg atggttactt
     2941 tatatgagtt accgttgatt ttatatttat attctttcat cgtttttact ttttaaaagg
     3001 tgacttactt tctgggagga gtttcacgca aagtgtaaat cttcgaactc catggtgagt
     3061 agctgcgttt tacgcgggtg atggtcagca ccgtttcttc tacgtcgtgc acctcatcct
     3121 gcatttcgtg taatgccata gaaatagcag cgtatacttc gcccggagcc tgtgacagtt
     3181 ccttagcttc atgcttgtca atgcctttag atttcatagc gttgcgcttg ctcaaattaa
     3241 cagctacttt tcctacaatc ttaaaagcaa taaagagaag aatcaatccg cagaatacta
     3301 cgctcatggc agaaatggac attccaacgc catcagcatc gtgttcttca aacttttcca
     3361 tcttggaatt gctgtcaaga gtcttgttgt tggtgctcgg agtcacgtat ttatcaccgc
     3421 ttccactttt tacttcccat tcagctgccc cgtcgcttac acgagcgtat gattggttgg
     3481 gaccgagcgc gcctgcgggc actacgatct ggtcgagcaa cttcttgccg gagtcgaaca
     3541 gacctaccca gttagctgtt tccggattca gcttgaagct ggtatggaaa gtaccacggt
     3601 taggttcgcc atctgcccag aatagtgcat gttggcgtgg tttcactaag gtaagtatat
     3661 cacctttcgg gataaaataa gtaactgtat cgcccggttg gctacttact ttcagcaagc
     3721 aagctgccag gtcggcgcta ccgaatgatt tattgaatat ttcaatccat gcgctgtgca
     3781 atccgtagtc atcttgaaaa ttattctgat tgtctatcag gatttcgttc agtaccagct
     3841 tgttgttcga cttttgctct ccacaagaag tcagcccaat cagcaacagc aaagaaagga
     3901 atattccgat tttggttttg ttcataatcg ttcttgtttt aatgtgaaaa gtaggattac
     3961 aacggaatat taccatgctt cttagccgga ttactcaatt tcttagtctg caactgctgc
     4021 aaagcacgga taacacggaa acgtgtgttg cgcggttcaa tcacatcgtc aatgtagcca
     4081 tatttagctg cattgtaagg attggcgaac aatttcgtgt attctgcttc tttttcagca
     4141 aggaattgag cgggattttc ctgatctttg gcttcgcgtg cgtacaatac ttctactgct
     4201 cctgctccac ccattactgc gatttcagca gtcggccatg cgtagttcat gtcaccgcga
     4261 agttgcttac agctcattac gatgtgagaa ccaccgtagg atttacgcaa tgtaatcgta
     4321 accttaggta cagtagcttc accgtaagca tacaatagct tagcaccatg aaggataacg
     4381 ccgttgtatt cctgacctgt tcccggaagg aatcccggta cgtctaccaa cgatacgata
     4441 ggaatattga atgcatcgca gaaacgaacg aaacgtgcac ccttacgaga tgcgttgctg
     4501 tcgagcacac cagccagata cttaggctga ttagcaacga tacctaccga ttggccgttg
     4561 aagcgggcga aaccgatgat gatattcttt gcataatctt tctggatttc aaggaattca
     4621 ccgttgtcca caatagcgcc gatcacttcg tacatgtcat acggtttagt agggctgtcg
     4681 gggataatat cgttcaaaga atcttccaga cggtcgattg ggtctgtgca atctacataa
     4741 ggagcttctt caaggttgtt ttgaggaata tagctcaaca gtttgcgaat cagttcgaaa
     4801 ccttcttctt ctgtttgggc ggtgaaatga gtcacacccg atttagtgga gtgaacgctt
     4861 gcgccaccga ggttttcctg gctaacgtct tcacctgtaa cggtcttcac cactttcggt
     4921 ccggtgagga acatataaga agtgccttcc atcatcagtg tgaagtcggt cagagccgga
     4981 gaataaacag caccaccggc acaaggaccg aagataccgg aaatctgcgg aataacacct
     5041 gaagcaagga tgttgcgttg gaagatttct gcataacctg ccaaagcgtt gataccttcc
     5101 tggatacgtg caccacccga gtcgttgata ccgatacagg gagcgcccat cttcatggct
     5161 tgatccatga ttttacaaat tttcaatgac atcgtttctg acagtgaacc ggcagaaaca
     5221 gtgaagtctt gtgcgaacac atataccaga cgtccttcga tcgtaccgca gccagttact
     5281 acaccgtctc ccgggtaatg ttttttctcc atgccgaagt tcgtgcatct gtgctcaacg
     5341 aacatgtcca tttcttcgaa gctaccctcg tcaagcagca tagccagacg ctcgcgtgct
     5401 gtgtatttcc ctttttcgtg ttgcttcgca attgcttttt caccgcctcc gatacgtgct
     5461 acggcacggc gttcaataag ctctttaatt ttttcaagtt gattactcat gagttcttgt
     5521 tagttttaga gtttatgatt aatgttcgca gagttctgtt aacacaccca aagttgattt
     5581 cgggtgaagg aaagcgatat tcaaaccttc agcacctttg cggggagctt tgtcgataag
     5641 gcggatttct ttgccttctg cctctgccag cgcattggct acgccatctt ctacagcaaa
     5701 tgcgacgtgg tgaacgcctg cacctttgtt ttcaatgaat ttggcaatag tactctccgg
     5761 gcaagtaggc tccaacagtt caatttttgt ttcgcccact tttaaaaaag cagttcttac
     5821 tttctgatct tccactgttt caatgttgta acacttcaga cctaatacat tttcgtagta
     5881 aggaagggct tcttcgatgc tttttacagc aatgcccaga tgttcaatgt gtgaaatctt
     5941 cataactata ctttttttat ttggttttaa atctgtgctt ataaggcata aaacagcaca
     6001 aagaaagaca tttcttatc
//
