LOCUS       c00289_gnlPro..         7101 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IFFMHHBN_289.
ACCESSION   c00289_gnlPro..
VERSION     c00289_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 06:42:25
            Original ID  :: gnl|Prokka|IFFMHHBN_289
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7101
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7101
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3667:6623]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3668..6623
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7101
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7101
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1364
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 81.2; E-value: 1.5e-25)"
                     /gene_kind="other"
                     /locus_tag="ctg289_1"
                     /sec_met_domain="Fn3-like (E-value: 9.5e-27, bitscore:
                     81.2, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.7e-40,
                     bitscore: 127.2, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GGSGFVTPYSVVSLYDGMVQLKGGRQIELLSDSILYKDMSQQIYT
                     DGSFTQNGFKGEYYNTRNLTGELFQAAIEPAIDHAWKYGAPFDGMPVDQFSARWTGVYK
                     ADKDGTVKFQLAGDDGYRLFVNDKLITGDWGNHSFSTRSAFMQVSAGEIYRLRIEYFDN
                     VGEATVSFQAGMMDEERLASSLKHARNVIVCAGFNSSTEGEGFDRPFALSYGQEYLINK
                     VASLHDNVAVVVNAGGGIDFRNWGQSVQAILMAWYPGQEGGKALAEIITGKLSPSGKLP
                     VSIEEKWEDNPVYGNYYDNRNVPHKRVQYAEGVFVGYRGYDRNGKQPLYPFGYGLSYSS
                     FEYSNLSVEKTGGSRVTVSFDIKNTGKMDAAEAAQVYVRDVECSVPRPLKELKGYDKVY
                     LKKGETKRVRILLDEEAFAYYDVESHRFVVEKGTFEILAGPSSADLPLKATVVL"
     CDS             1395..3599
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 84.6; E-value: 1.3e-26)"
                     /gene_kind="other"
                     /locus_tag="ctg289_2"
                     /sec_met_domain="Fn3-like (E-value: 8.2e-28, bitscore:
                     84.6, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.5e-75, bitscore:
                     242.6, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3e-53, bitscore:
                     168.9, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIILMVCLWGLGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQ
                     LAQCTYRGNFTGPDGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRLRIPLIF
                     GLDVIHGYRTGFPLPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARW
                     GRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVD
                     MSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVV
                     SDYNSVEELVNHRYAVDKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDD
                     AVRRILEKKYELGLFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLP
                     LTAQTRKIALIGGLSKSQYDMAGAWAATTDRNKVVTLYDALVRKGLEVSYAEGYDMKSN
                     QLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVI
                     ALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQ
                     IPIYYQNKSTGRPTALKQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKAAGV
                     HTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNVGESKTVAFQIT
                     DDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(3668..4393)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg289_3"
                     /sec_met_domain="Radical_SAM (E-value: 6e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(4395..6623)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg289_4"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1169.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gaggaggaag cggatttgtc accccttatt cggtagtgtc actctatgac ggtatggttc
       61 agttgaaggg cggcagacag atagagctgc tgtctgacag cattctttat aaggatatgt
      121 cccaacagat ttatacggat ggcagtttta cgcaaaacgg ttttaagggc gaatattata
      181 atactcgtaa tcttaccgga gaactctttc aagctgccat agagcctgcc atagaccatg
      241 cctggaaata cggtgcaccg ttcgacggca tgccggtaga tcagttttct gcccgttgga
      301 cgggagttta caaggctgac aaggatggaa ctgtcaagtt ccagctggct ggcgatgacg
      361 gataccgtct gtttgtaaac gataagctga ttaccggaga ttgggggaac cattcattca
      421 gtacccgcag tgcatttatg caggtcagtg caggggagat ataccggttg cgtattgaat
      481 attttgataa tgtgggtgag gcgactgtca gtttccaggc aggaatgatg gatgaggagc
      541 gtttggcctc ttcactgaag catgccagga atgttattgt atgtgccggg ttcaacagca
      601 gtacagaagg tgaagggttc gaccgtccgt ttgcattgtc atacgggcag gaatatctca
      661 ttaataaagt ggcttcactt catgataatg tggctgtggt ggtgaatgcc ggaggtggaa
      721 tagatttccg caattggggg cagagtgtac aagctatttt gatggcatgg tatccggggc
      781 aggagggtgg aaaggccctt gccgaaatca ttacgggcaa gctttcgcca agtggcaaac
      841 taccagtcag catcgaggaa aaatgggaag ataatccggt atatggtaat tattatgaca
      901 atcgcaatgt acctcataaa cgggtgcagt atgccgaagg tgttttcgta ggttataggg
      961 gatatgaccg gaacggaaag caaccgctct atcctttcgg gtatggactg tcatacagta
     1021 gttttgaata tagtaatctg agtgttgaga agacgggggg aagccgggtt acggtaagct
     1081 ttgacatcaa gaataccgga aagatggatg ctgcggaagc ggctcaggta tatgtgcggg
     1141 atgtggaatg ttctgttccg cgtccattga aggagttgaa aggctacgat aaggtttact
     1201 tgaagaaggg tgaaaccaag cgcgttcgga tattgctgga tgaggaagcc tttgcctact
     1261 atgatgtgga atcacatcgg tttgtagtgg aaaaggggac ttttgagatt ctggcaggtc
     1321 cttcttcagc tgatttacca ttgaaggcaa ctgttgtttt atgataaaat aaatatcaaa
     1381 acaagaatgg cagtatgaaa aagataataa tactgatggt ttgtctgtgg ggacttggta
     1441 acacctttac ctcggcacaa actacggaga aggagaaatt catctcaagc ttgatgaagc
     1501 agatgacgct ggatgaaaag ataggccagc ttgcacaatg cacctaccga gggaatttta
     1561 ccggacccga cggtggaaat attcctaaag aagagtatgt ccggcaaggc agaataggct
     1621 ctatgctgaa tgtgatagga gtaaaggata tacgccgtta tcaagagctt gctttgcaat
     1681 cgcgtttgcg cattccttta attttcggac tggatgttat ccacggctat cgtaccggat
     1741 ttccgcttcc gcttgccgag gctgcaagtt tcgatttggc catgattgag gaagctgcac
     1801 gctacaatgc tttggaaagt gccgctgatg gtttgaactg ggtttttgcc ccgatggtgg
     1861 atatttcgtg ggacgcacgt tggggacgcg tcatggaagg ggccggtgag gacccttatt
     1921 atggttctcg ggtagcggag gcgcgtgtca gaggcttgca aggagacgat ttggcagaca
     1981 cgacgactgt catggcatgt atgaaacatt ttgccggata tggtgcaccg atagcaggaa
     2041 aggagtataa tagtgtggat atgtcaatgg gccactttgc caatttttat atgcctccct
     2101 ataaggcggc cataaaggta ggagcagcta ccgtgatgag tgctttcaat gattttaata
     2161 atattccttg tactgccaat gatttcttgc tcaaccggct attgagagag cagtggggat
     2221 ttaagggatt tgttgtgtcc gattataatt cggtagagga attggtgaac caccgttatg
     2281 ctgttgacaa gaaagatgcc gcagccaaag cgctgaatgc tggtctggac atggagatgg
     2341 taagtacctg ctacttgact tatctgaagg agcttgttca agaagggaaa gtaaaggaga
     2401 gtgtgcttga tgatgcagta cggcgtatat tggagaagaa atatgagttg ggattgtttg
     2461 aggacccttt tcgctactgt aatccggaaa gagcggccag aatactcaat gcgcccgaag
     2521 tccgtaatgc ttcgctccgt atggccgagc gttccgttgt attgctggag aatagggaat
     2581 ctgtgcttcc gttgacggca cagacgagga agatagcctt gataggagga ctctcaaaat
     2641 cccagtacga tatggcaggt gcttgggctg ccactacgga ccggaacaaa gtggtgacct
     2701 tatatgatgc attggtccgg aaaggactgg aggtgagcta tgctgaaggc tatgatatga
     2761 agagcaatca gttgaccgga ttgcaggaag cattacaggc tgctgaggct tcggatgtca
     2821 ttgttgtggc ggtaggcgag agattcggtc agagtggcga gaaggcttcc aaagtgaata
     2881 tagccatacc cgaggggcag cagaagctgg tgtcggaact ggcaaagact ggaaaacctg
     2941 tcattgctct tgtcatgtgt gggcgtccgg taatcttcaa tgagataagg gaacatgctg
     3001 atgcggtact gtgtacttgg tggctgggaa gtgaggccgg caatgccata tgcaatgtgt
     3061 tgtggggaga gtataatcct tcggctaaat tgcccatgac atttcctgca cacgtaggac
     3121 agattcccat ttattatcag aataagagca ccggccgccc taccgcattg aaacagacct
     3181 ataaggcatc ttatatcgat gcttccactg agcctgccta tcctttcgga tatgggcttt
     3241 cgtacacgga ttttggatat tctgatttga aactgctgcc gggcaaggct gcaggcgtcc
     3301 atacggaggt gtcggtaaaa gtgactaata ccggtaagtg tgcaggagag gaagtagttc
     3361 aattgtatgt gcaggataag gtagcctctg ttacgcgtcc gataaaggaa ttgaagggct
     3421 tcaggaaaat tcatctgaat gtgggtgaaa gcaaaactgt agcttttcag ataactgacg
     3481 atgcactggg attctatgac aatgagatga attatatggt agaacctggt gatttcgtct
     3541 ttatggtggg tggcagtagt gatgatgtac agcagattac ctataacttg aaagaatagg
     3601 attcttaccc tataaatgaa aagcaaggct gtctggaatc atatccggac agccttgctt
     3661 ttctgtttta gttggttatc acattcgtga aatactcttt gaacagtttt tctgcccgtt
     3721 ccagttgttc cggagtattt tccttcactt ctttcaattt gtaatcccat cccatggctt
     3781 cgtatttgtg aacacccaga cggtgataag gaagtatttc cacccgttgt atctgctggt
     3841 atttgccaag actttcaccc agcaggcggg tgtcttcttc cgaatcgctg taaccgggga
     3901 ccagtacata gcgcagccag aaggggtgtt catgttcttc gagccaggca gcggtgcgga
     3961 gtgtctgttc gttgcttcgt cccgtcagcg tacggtggcg ttcgggattg aattgcttga
     4021 tgtccagcag cacgaggtca gtcaggctaa agagctcttc caccttttca ttccagatgc
     4081 ctccgttact gtccagacag atatggatgc cgttcgcctt cagctccttg aaaagaggaa
     4141 tcagcgcttc tgcttgcagg gtaggctcac cgccggagaa agtaatacct cctttcttcc
     4201 cgaagaatgc cttttggctg atagccattt gcagaatctc ttccggagtg gtgggagtgc
     4261 ctccttttac atcaatcgta tccggattgg cacaatacag acaacggaac ggacatcctt
     4321 gcaggaaaac gacgagccgg agacccggcc cgtcgaatgt tcccatactt tcgtaggagt
     4381 gtacatttat catattacat tctttcgtgg aatgaacggc tgatgacttc caactgatgc
     4441 tcgcggctca acttcacgaa gttcacggca tagccggaaa cgcggatggt gagttgcgga
     4501 tacttttcag gatgttccat ggcatcttcc agcatttcgc ggttcagtac gtttacgttc
     4561 aagtggtggg cacctttggt gaagtagccg tccatcatgg ttaccaggtt ctctacacgt
     4621 tcttccggtg tcgggcccag tgatttggga acaatggaga acgtattgct gatgccgtcc
     4681 tgcgagtcgc ggtagcgcaa tttggctacg gagctcaagg aagcgatggc accgttcttg
     4741 tcacgtccgt gcattgggtt ggcacccgga gcaaaggcaa cacctttggc gcgtccgtcc
     4801 ggagtggcac cggtcttctt gccgtacatc acgttggagg tgatagtcag caaggaaagg
     4861 gtgggacggg cattcttgta gacaggcagc tttttcagtt cttcgctgaa atagtaaacg
     4921 aggtctactc ccaggtggtc cacacggtcg tcattattgc cgaagcaggg gaatgaacct
     4981 tcagtgtcga aaccttcggt caggccgatt tcgttgcggc gtgcagtgac tttggcgtat
     5041 ttgatggcag acagtgagtc tatggcgatg gaaaggccgg caacaccgta tgccagattg
     5101 atgcgtgggt cggtatcgat gaatgccatc tgggctttct cataataata cttatcatgc
     5161 atgtagtgga tgatattcat ggcctcgttg tagacacggg ctatttcagt cagtaccttc
     5221 ttgtagttgg ccataacttc ctcaaacttc aatgtctcac cggtcaatac gggaatacct
     5281 ttcaccatga cagttccggt attttcgcaa cgtccaccgt tgatggcgag cagcagggcc
     5341 ttggcaaggt tggcacgtgc gccgaagaac tggatttgtt tgccgatggc ttggtaggat
     5401 acacagcagg cgatgccgta gtcgtcgcag ttgcgcactt cacgcatcag gttgtcgttt
     5461 tcgtactgga tggagctggt atctacagaa actttggcgc agaaatcctt gaagccttcc
     5521 ggcagttcgg gactccacaa tacggtcaag ttcggttccg gagaaggacc gaggttgtat
     5581 agcgtctgca aaaagcggaa agaagtcttg gtgaccttca cgcgtccgtc attgaagcgt
     5641 cctccgatgg cttcggtcac ccaagtcggg tcaccggcaa agatatcatt gtatgattgc
     5701 atacgcagat ggcgtaccat gcgcagcttg atgacgaact ggtctatcag ttcctgtgca
     5761 aacgtctcat tgatttttcc gtgagccaga tcgtattcaa tgaatatatc gaggaacgaa
     5821 gatacgttac ccagtgacat ggctgctccg tcctgctctt tgatggcagc aagatatgcc
     5881 atgtataccc attgcacggc ctcttgagct gatgtagccg gatggctgag gtcgagtccg
     5941 taatattcgc ccataacctt gatgtccttc aaagctttga tttgttctgc cacttcttcg
     6001 cgtagacgga tgcgtgcttc ggtcatcggg ccggtgaggt tgcgcaagtc ctcctgcttg
     6061 gcttcaatca gacggtcgat gccgtagaga gccaatcggc ggtagtcgcc gatgatacgt
     6121 ccgcgggcat agttgtcggg caatccggta aggaatccca gtgagcggaa ggaacggatt
     6181 tcttcggtat atacatcgaa tacgccgtcg ttatgtgttt tgcggtaatg ggtgaagatg
     6241 tcttttactt tatcgtccac ttccactccg ttttcacggc atgcttttgc taccacgttg
     6301 atgcctccga aaggtttgat ggatcgtttc aacagttcat cagtctgcaa accgacaata
     6361 agttcattgt ccttatcaat atacccggct ttatgagaag tgatggtaga tacggtggta
     6421 ttgtccagtg aacggacgcc attgttgttc ctttcttctt cgagagcctt cagacagatg
     6481 tcccagatac gtttggtacg ttcggtaggt ccctgcagga aagatgcatc tccggtatag
     6541 ggtgcgatgt ttttactgac gaaatcactg acattgattt ctttattcca aagcccattg
     6601 gtaaaggttt tgttcaattc cataaaattt gtttttgtta gtaggttaga taatgttatc
     6661 ctttagagcg cgcaaaaata ctgaataaat tccgtaattt tataagagat acttctcttt
     6721 ttatgttgaa cagcataaaa taactattat taggtagtga atgtccgggg gagtagagat
     6781 gttaatactt tctttatatg tggtttatga attgaaacaa gaaccgaata ataatctgca
     6841 ttatttacaa atgatgtagg tttaaggaac agtcgtattc tgaacctaat tcagtcgtat
     6901 agtgaatgcc attcagtcgt atactgaata gggttcaatc gtatactgaa tgtggctctt
     6961 tgtaaatata ctgatagcca gtaagttata aaactctgtc ttgcccttga taaatttcaa
     7021 caaatagcct aaatgccgtt atgttcgttc cgtagtgggt ataaacaaga attttggtat
     7081 aattgctctg tttttgtgca g
//
