LOCUS       c00136_gnlPro..         4084 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KEEDCBCM_136.
ACCESSION   c00136_gnlPro..
VERSION     c00136_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 03:42:28
            Original ID  :: gnl|Prokka|KEEDCBCM_136
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4084
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4084
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[836:3828]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      837..3828
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4084
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4084
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             837..3089
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_1"
                     /sec_met_domain="Gly_radical (E-value: 3.8e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             3076..3828
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTECHKTLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             4009..4083
                     /locus_tag="ctg136_3"
                     /transl_table=11
                     /translation="MDKISPEEKIQWMKKIFQKKESISS"
ORIGIN
        1 cctgttccca gtcattcttg agtaagcaga agacatcagt ttctgtcccc aaatcccctc
       61 cctctgttta attcatcttc tccagccata gcacagaagc cattgccccc aataaggcat
      121 atttgtggtc tcggagcaag tgttggatgc gagcgagtag agcgaacatt caacacgccg
      181 cgactaaaat cacaaatatg ccttattcgg ggcaatggct tcgtctgtag ttcgcttaat
      241 gaattaaata gtaacccctt gacattttgt tctattttgt ttatagttag aatacttata
      301 cgactgacgg aagtaagtgg aatgaaccac atgaagtata agactcttat agccgaccgt
      361 ctgggcaatt cttgtctgga tgctggcttt tttatttctg ataagataat attctatcca
      421 atatccctga taagaagtaa ctttaaatcg ttattaagtc acgtgctaaa gaagatgttt
      481 ttccaaacat gctctaaaga gcgaaaaata aaattatagt tgtatgcttg tcattattgt
      541 acttatatca gtacatttgt acgatttgat aatattttat caacgctcta aaacccttta
      601 ttttaaaggc tctacaagca tttttgtgta tatgttcaaa aaaaagaaca cataatatca
      661 aaaaattgtt tgacgattgc caaaaatcat gttaataatg ttgttagatg attaaaactt
      721 aaataagtta aaactaatca tagtaaatca tctgaacaat tttgttgatt ttttagcaaa
      781 catatttcaa aaccttgact taaggatttt tttaacaaac agggaggtca attaaaatgt
      841 tacaaaaaga acaatggcag ggatttaatg gacgaatctg gagagaagaa attaatttaa
      901 gagagttcat ccaggataac tacactccat atgatggaga tgcttctttc ctcgaaggcc
      961 ctaccgaggc tactgacaca ctgtggggcg aactgcagaa gctgcagaaa gcagagcgtg
     1021 aaaagggtgg tgttcttgat atggatactg atatcgtatc aagccttact tcccataaac
     1081 cgggttacat ttctgagtct ttaaaagaga aagaacagat cgtaggtctt cagacagata
     1141 aacctttaaa acgtgcattt atgccatttg gtggtatccg tactgctgaa gaagcttgta
     1201 caacttatgg ttacacacca aatccagaat tccacaagat ttttacagat tatcataaga
     1261 cacataacca ggcagtattt gattcttata ctcctgaaat gaagaaagct cgtcacaata
     1321 agatcattac tggtcttcca gatacttacg gacgtggacg tatcgttggt gactaccgtc
     1381 gtgtagctct ttacggtatt gacctgttag ttgctgacaa gcaaaaagat ttcgcaaact
     1441 gcggtgacgg aacaatgaca gatgacgtta tccgtttaag agaagaaatc tccatgcaga
     1501 ttaaagcatt aaaagatatg aaagttatgg ctgaaagcta tggctatgat atttccgaac
     1561 ctgcttctaa cgcaaaggaa gctgttcaat ggacgtattt cggatacctt gcagcaatca
     1621 agacacagaa cggtgctgct atgagtatcg gacgtatcgc tactttcctt gacatttata
     1681 tcaaacgtga tatggataag ggaatcctta cagagaaaga agcgcaggaa ttaatcgacc
     1741 atatgactat gaagttccgt atggttaagt tcggacgtat cccagcttac aaccagctct
     1801 tctccggtga ccctgtatgg gcaacacttg aagttggcgg acttggacag gacggtcgtt
     1861 ccatggttac aaaaactgac taccgtttcc tccacacatt agaaaatatg ggaccatccc
     1921 cagagccaaa cctcacagta ctttactctg agcgtcttcc aaaagcattt agagattacg
     1981 cagctcatat ttctattaca acaagttcaa tccagtatga aaatgatgat gtaatgcgtc
     2041 cagtatgggg cgatgattac tccatctgct gctgtgtttc tgctacacag actggtaagg
     2101 aaatgcagtt cttcggagcc cgtgcaaacc ttgcaaaatg tctcctctac gcaatcaatg
     2161 gtggtgtaga tgaaaagact ggtcagcaag ttggtccgga atacaaacca attacttccg
     2221 aatatcttga ctatgatgaa gtaatgcaca aatatgatat catgatggac tggttagcag
     2281 gtttatatgt aaatacatta aatctcatcc agtacatgca tgacaaatat tactatgaat
     2341 atgctttgat ggccttaatc gatacaaatg tacgtcgtac attcgctaca ggtatcgcag
     2401 gattctcaca tgtagtagat tccttaagtg cgattaaata tgccaaagta aaaacagtcc
     2461 gcaatgaaga aggacttgtt gttgattatg agacaactgg cgacttccct aaatacggaa
     2521 atgatgatga ccgcgctgat gatatcgctg tatggttact tcagacattt atgaagaagc
     2581 ttgaaaaatt ccatacttac cgtgattctg aacctacaac ttccatcctt actatcacat
     2641 caaacgtagt atatggtaag gcaactggtt cccttccaga cggaagaaag gccggagaac
     2701 ctctttcccc aggagcaaac ccaagttatg gtgctgagca gaacggactt ttagcttcct
     2761 taaactctgt agcaaaactt ccttacgaat gggcattaga tggtatttcc aatacacaga
     2821 caatcaaccc tgatgcactc ggtcattccg aagaagaacg tatcaataac ttagtaaatg
     2881 ttatggacgg ctacttcgca cagggtgctc accacctaaa cgtaaatgta tttggtgttg
     2941 agaaactgat tgatgctatg gaacatccgg aaaaagaaga atacgcaaac tttacgatcc
     3001 gtgtttcagg ttatgcagtt aaattcatct ctctgacaaa agaacagcag ttagatgtaa
     3061 ttgccagaac atgccatgac agaatgtaat aaaaatgcga tggctcttgt tcattctacg
     3121 gagagttttg gttcggtgga tgggccggga gttcgtttta ttgttttttt acagggttgt
     3181 cctttgcgat gtcagttctg tcacaatcca gatacatgga agatgacaga ggaaaatggt
     3241 gcggtgtgga gaagtgcgga tgagctgtta aatcaggcac tccgttatcg tacttactgg
     3301 aaaaatagcg gcggtattac cgtaagtggt ggtgagcctc ttttacagat tgattttatg
     3361 ctagagtttt ttaaaaaggc aaaagaaaaa ggaatccaca ctgttattga tactgctgga
     3421 ggacctttta caagagagga acctttcttt tctaaatttc aggaattgat gaaatataca
     3481 gacctgttat tagtggatat caaacatatt gatactgaat gtcacaagac tttaactggt
     3541 cgaagcaacg aaaatattct cgacatgatt cgctatcttt ctgatatcaa aaaaccaatt
     3601 tggattcgtc atgtccttgt tccggaacga agcgataaag atgaatatct gactagatta
     3661 gctgatttca ttcactctct tgataatgtg gaaaaggtgg aaatcctgcc ttatcatacg
     3721 atgggaattt ataaatggaa agaacttgga ttagagtatc cactggaggg aattgagcca
     3781 ccaacaaaag aacgagtgga gaacgctaag aaaattcttg ctatttgatt tgccgacaaa
     3841 cagaatccta aaaaccccca tatttgtgac tttgaagata gattgcgttg aatacttatt
     3901 catcttcaat ctatccttga agtcacaata tggggggtct ctggtatgct cccttttagg
     3961 tagacagtta aaaaaataaa actgtttatc tgaaaggggg cattttttat ggataagatt
     4021 tctccagaag aaaaaataca atggatgaaa aaaatttttc agaagaaaga atctattagt
     4081 tctg
//
