LOCUS       c00078_gnlPro..         4359 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HONLNNHN_78.
ACCESSION   c00078_gnlPro..
VERSION     c00078_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 07:08:56
            Original ID  :: gnl|Prokka|HONLNNHN_78
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4359
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4359
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1155:4147]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1156..4147
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4359
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4359
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1156..3408
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg78_1"
                     /sec_met_domain="Gly_radical (E-value: 2.5e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.8e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             3395..4147
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg78_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.2e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTECHKTLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
ORIGIN
        1 atatttgtga ctttagtcgc tgcggtttaa atgctcgctt tatgcgctcg catctaaacc
       61 ttgctccgag gccacaaata tagggtatcg gttctcttcc cgggctgtcc ttgtctggat
      121 tttgtttgaa ttaaatagtg gttgtgggga tttgggggag agggtagaag ttttctgctt
      181 gctcttaaaa tattgtgaat agatgttttg tatttaaata gaagaggtaa tgaattgata
      241 ttgataacaa cagtctgctt tttattctga aattattgaa atattgctga gataaccatt
      301 aaaaaagcta aatcatcccc ctgttcccag tcattcttga gtaagcagaa gacatcagtt
      361 tctgtcccca aatcccctcc ctctgtttaa ttcatcttct ccagccatag cacagaagcc
      421 attgccccca ataaggcata tttgtggtct cggagcaagt gttggatgcg agcgagtaga
      481 gcgaacattc aacacgccgc gactaaaatc acaaatatgc cttattcggg gcaatggctt
      541 cgtctgtagt tcgcttaatg aattaaatag taaccccttg acattttgtt ctattttgtt
      601 tatagttaga atacttatac gactgacgga agtaagtgga atgaaccaca tgaagtataa
      661 gactcttata gccgaccgtc tgggcaattc ttgtctggat gctggctttt ttatttctga
      721 taagataata ttctatccaa tatccctgat aagaagtaac tttaaatcgt tattaagtca
      781 cgtgctaaag aagatgtttt tccaaacatg ctctaaagag cgaaaaataa aattatagtt
      841 gtatgcttgt cattattgta cttatatcag tacatttgta cgatttgata atattttatc
      901 aacgctctaa aaccctttat tttaaaggct ctacaagcat ttttgtgtat atgttcaaaa
      961 aaaagaacac ataatatcaa aaaattgttt gacgattgcc aaaaatcatg ttaataatgt
     1021 tgttagatga ttaaaactta aataagttaa aactaatcat agtaaatcat ctgaacaatt
     1081 ttgttgattt tttagcaaac atatttcaaa accttgactt aaggattttt ttaacaaaca
     1141 gggaggtcaa ttaaaatgtt acaaaaagaa caatggcagg gatttaatgg acgaatctgg
     1201 agagaagaaa ttaatttaag agagttcatc caggataact acactccata tgatggagat
     1261 gcttctttcc tcgaaggccc taccgaggct actgacacac tgtggggcga actgcagaag
     1321 ctgcagaaag cagagcgtga aaagggtggt gttcttgata tggatactga tatcgtatca
     1381 agccttactt cccataaacc gggttacatt tctgagtctt taaaagagaa agaacagatc
     1441 gtaggtcttc agacagataa acctttaaaa cgtgcattta tgccatttgg tggtatccgt
     1501 actgctgaag aagcttgtac aacttatggt tacacaccaa atccagaatt ccacaagatt
     1561 tttacagatt atcataagac acataaccag gcagtatttg attcttatac tcctgaaatg
     1621 aagaaagctc gtcacaataa gatcattact ggtcttccag atacttacgg acgtggacgt
     1681 atcgttggtg actaccgtcg tgtagctctt tacggtattg acctgttagt tgctgacaag
     1741 caaaaagatt tcgcaaactg cggtgacgga acaatgacag atgacgttat ccgtttaaga
     1801 gaagaaatct ccatgcagat taaagcatta aaagatatga aagttatggc tgaaagctat
     1861 ggctatgata tttccgaacc tgcttctaac gcaaaggaag ctgttcaatg gacgtatttc
     1921 ggataccttg cagcaatcaa gacacagaac ggtgctgcta tgagtatcgg acgtatcgct
     1981 actttccttg acatttatat caaacgtgat atggataagg gaatccttac agagaaagaa
     2041 gcgcaggaat taatcgacca tatgactatg aagttccgta tggttaagtt cggacgtatc
     2101 ccagcttaca accagctctt ctccggtgac cctgtatggg caacacttga agttggcgga
     2161 cttggacagg acggtcgttc catggttaca aaaactgact accgtttcct ccacacatta
     2221 gaaaatatgg gaccatcccc agagccaaac ctcacagtac tttactctga gcgtcttcca
     2281 aaagcattta gagattacgc agctcatatt tctattacaa caagttcaat ccagtatgaa
     2341 aatgatgatg taatgcgtcc agtatggggc gatgattact ccatctgctg ctgtgtttct
     2401 gctacacaga ctggtaagga aatgcagttc ttcggagccc gtgcaaacct tgcaaaatgt
     2461 ctcctctacg caatcaatgg tggtgtagat gaaaagactg gtcagcaagt tggtccggaa
     2521 tacaaaccaa ttacttccga atatcttgac tatgatgaag taatgcacaa atatgatatc
     2581 atgatggact ggttagcagg tttatatgta aatacattaa atctcatcca gtacatgcat
     2641 gacaaatatt actatgaata tgctttgatg gccttaatcg atacaaatgt acgtcgtaca
     2701 ttcgctacag gtatcgcagg attctcacat gtagtagatt ccttaagtgc gattaaatat
     2761 gccaaagtaa aaacagtccg caatgaagaa ggacttgttg ttgattatga gacaactggc
     2821 gacttcccta aatacggaaa tgatgatgac cgcgctgatg atatcgctgt atggttactt
     2881 cagacattta tgaagaagct tgaaaaattc catacttacc gtgattctga acctacaact
     2941 tccatcctta ctatcacatc aaacgtagta tatggtaagg caactggttc ccttccagac
     3001 ggaagaaagg ccggagaacc tctttcccca ggagcaaacc caagttatgg tgctgagcag
     3061 aacggacttt tagcttcctt aaactctgta gcaaaacttc cttacgaatg ggcattagat
     3121 ggtatttcca atacacagac aatcaaccct gatgcactcg gtcattccga agaagaacgt
     3181 atcaataact tagtaaatgt tatggacggc tacttcgcac agggtgctca ccacctaaac
     3241 gtaaatgtat ttggtgttga gaaactgatt gatgctatgg aacatccgga aaaagaagaa
     3301 tacgcaaact ttacgatccg tgtttcaggt tatgcagtta aattcatctc tctgacaaaa
     3361 gaacagcagt tagatgtaat tgccagaaca tgccatgaca gaatgtaata aaaatgcgat
     3421 ggctcttgtt cattctacgg agagttttgg ttcggtggat gggccgggag ttcgttttat
     3481 tgttttttta cagggttgtc ctttgcgatg tcagttctgt cacaatccag atacatggaa
     3541 gatgacagag gaaaatggtg cggtgtggag aagtgcggat gagctgttaa atcaggcact
     3601 ccgttatcgt acttactgga aaaatagcgg cggtattacc gtaagtggtg gtgagcctct
     3661 tttacagatt gattttatgc tagagttttt taaaaaggca aaagaaaaag gaatccacac
     3721 tgttattgat actgctggag gaccttttac aagagaggaa cctttctttt ctaaatttca
     3781 ggaattgatg aaatatacag acctgttatt agtggatatc aaacatattg atactgaatg
     3841 tcacaagact ttaactggtc gaagcaacga aaatattctc gacatgattc gctatctttc
     3901 tgatatcaaa aaaccaattt ggattcgtca tgtccttgtt ccggaacgaa gcgataaaga
     3961 tgaatatctg actagattag ctgatttcat tcactctctt gataatgtgg aaaaggtgga
     4021 aatcctgcct tatcatacga tgggaattta taaatggaaa gaacttggat tagagtatcc
     4081 actggaggga attgagccac caacaaaaga acgagtggag aacgctaaga aaattcttgc
     4141 tatttgattt gccgacaaac agaatcctaa aaacccccat atttgtgact ttgaagatag
     4201 attgcgttga atacttattc atcttcaatc tatccttgaa gtcacaatat ggggggtctc
     4261 tggtatgctc ccttttaggt agacagttaa aaaaataaaa ctgtttatct gaaagggggc
     4321 attttttatg gataagattt ctccagaaga aaaaataca
//
