GCA_017963505.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaCJGJPBOO_1 (original name was: gnl|Prokka|CJGJPBOO_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	cyclic-lactone-autoinducer	21,821	42,526

gnlProkkaCJGJPBOO_56 (original name was: gnl|Prokka|CJGJPBOO_56)

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	NRPS	4,735	37,261

gnlProkkaCJGJPBOO_65 (original name was: gnl|Prokka|CJGJPBOO_65)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	RiPP-like	1	10,539

gnlProkkaCJGJPBOO_68 (original name was: gnl|Prokka|CJGJPBOO_68)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	NRPS,NRPS-like	1	23,132

gnlProkkaCJGJPBOO_114 (original name was: gnl|Prokka|CJGJPBOO_114)

Region	Type	From	To	Most similar known cluster		Similarity
Region 78.1	T3PKS	65,202	96,286

gnlProkkaCJGJPBOO_125 (original name was: gnl|Prokka|CJGJPBOO_125)

Region	Type	From	To	Most similar known cluster		Similarity
Region 89.1	terpene	26,028	46,909

Region	Type	From	To	Similarity
Region 1.1	cyclic-lactone-autoinducer	21,821	42,526
Region 20.1	NRPS	4,735	37,261
Region 29.1	RiPP-like	1	10,539
Region 32.1	NRPS,NRPS-like	1	23,132
Region 78.1	T3PKS	65,202	96,286
Region 89.1	terpene	26,028	46,909

Compact view

gnlProkkaCJGJPBOO_1 - Region 1 - cyclic-lactone-autoinducer

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Location: 21,821 - 42,526 nt. (total: 20,706 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

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gnlProkkaCJGJPBOO_56 - Region 1 - NRPS

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Location: 4,735 - 37,261 nt. (total: 32,527 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 4734 - 37261:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg20_23

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AMP-binding (8..381): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SVTQAAVAGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1000..1372): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSHGYGCVLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1967..2333): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLIVAAVIK
Nearest Stachelhaus code(s):: Ala DVWVVAAVIK (68% 8Å match); S-bohPhe or Phe DALIVAAVCK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2921..3307): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaCJGJPBOO_65 - Region 1 - RiPP-like

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Location: 1 - 10,539 nt. (total: 10,539 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

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gnlProkkaCJGJPBOO_68 - Region 1 - NRPS,NRPS-like

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Location: 1 - 23,132 nt. (total: 23,132 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 23132:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg32_16

AMP-binding (11..374): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VVYNTAVCAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ctg32_19

Search NORINE for peptide: strict or relaxed

AMP-binding (124..502): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DITQMGIVWK
Nearest Stachelhaus code(s):: Gly DILQMGMVWK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaCJGJPBOO_114 - Region 1 - T3PKS

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Location: 65,202 - 96,286 nt. (total: 31,085 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaCJGJPBOO_125 - Region 1 - terpene

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Location: 26,028 - 46,909 nt. (total: 20,882 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

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resistance

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Gene details

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Gene overview

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.