LOCUS       c00272_gnlPro..         5840 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AMEPOAEB_272.
ACCESSION   c00272_gnlPro..
VERSION     c00272_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 07:24:58
            Original ID  :: gnl|Prokka|AMEPOAEB_272
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5840
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5840
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1116:5839]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1117..5839
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5840
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5840
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             399..1070
                     /locus_tag="ctg272_1"
                     /transl_table=11
                     /translation="MESTISAAPQISIDREKTLRSHRRYWVLRRAQDIVFSLLALILLA
                     PMALLISLAIVLDSPGDGAIFRQRRVGRDGKLFWLYKFRTMCPDAEEQLNELLSQNQMD
                     GPVFKIEGDPRITRVGRFLRKTSLDELPQLLNVLQGDMSIVGPRPALPREVELYSDYQR
                     QRLYVTPGLSCYWQIAPHRNEMSFDEWVALDLKYIQERSFWVDWKIIFLTVRAMLMKYG
                     E"
     CDS             complement(1117..2775)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg272_2"
                     /sec_met_domain="ILVD_EDD (E-value: 3.6e-224, bitscore:
                     733.4, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSDNVTKGSERAPNRSLFYALGYTPEELERPLIGVVSAYSEIVP
                     GHMNLDKIAEAVKAGVQMAGGTPILIPAIGVCDGIAMGHVGMKYSLASRELICDSVETM
                     LMAHQLDGLVLVPNCDKIVPGMVMAAVRMDVPAVVCSGGPMLAGTYGGEEVSLSKMFEA
                     VGAYKAGMITDEQLEDCTCNCCPSCGSCSGMYTANSMNCLCEAIGIALPGNGTIPAVYS
                     KRLQLAKHAGMAVMEMVRKGITARQIINERSIRNALTCDMALGCSTNTVLHLLAIAYEA
                     GVPIDLKLFNEISAKTPNLCHLAPAGPTHMPDLYAAGGIPAVQAELAKKGLLDLEVPTV
                     TGKTLGENIKGAHILNEKAIRPIENPYSETGGLQILWGNIAPDGCVVKRSAVAPEMQQH
                     SGPARVFNSEEEAIAAIYAGKIVPGDVVVIRYEGPKGGPGMREMLNPTSALAGMKLDKT
                     VALITDGRFSGASRGAAIGHVSPEAASGGPIGLIEEGDTISIDIPNASITLEVSDAVLA
                     ERKAKYVAPEPNIKTGWLSRYARMVTSANLGAVLK"
     CDS             complement(2942..3961)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg272_3"
                     /sec_met_domain="IlvN (E-value: 3e-75, bitscore: 239.8,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 3.7e-66, bitscore: 210.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIKKYYDSDCNLGVLDGKTVAVIGFGSQGHAHSENLAESGVNVV
                     VGLRKGSAHWAKASEFAATHENFKVMEVEEAAKAGDVVMMLVPDELCADIYNTQVAPYM
                     TEGKALAFAHGFNIHFKTITAPKNVDVIMIAPKGPGHIVRRLYTEGEGCPSLICVEQDY
                     TGKAKDIALAYASGIGAGRAGILQTTFKEETETDLFGEQAVLCGGVSELIHAGFDTLVE
                     AGYEPEMAYFETCHEMKLIVDLINEGGFSKMRYSISNTAEYGDYRTGKRLITEETRKEM
                     KKVLTEIQDGTFASEFLTEMSPKGGRKVHFLAKRRMEAELPIEKVGAELRSMMSWLKK"
     CDS             complement(4061..4624)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg272_4"
                     /sec_met_domain="ALS_ss_C (E-value: 6.4e-29, bitscore:
                     88.6, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESDQRRVISLLVDNQSGVLARVSSLFCRRGFNIDSLTVSATNDP
                     AVSRITVTITSDEKALQQLVLQTERLEVTRQVFVLDSEKALERELLLLKVESDVHNRTE
                     LREIACIYKAKIIDLSPDSMVFELIGRPDKLDAFLKMFEGYNILEQCRTGVTALERGGM
                     HQHIQKPPQEVRSVQLPPPGARRA"
     CDS             complement(4640..5839)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg272_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.4e-52, bitscore:
                     164.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PVLVDIPKDVTAAVCEFTPKQPEPIRTVTTYNEEQVHWAADLINA
                     AQRPLVYFGGGVRSAASCQPLRDLLHKAEIPATYTLMAAGVVPYGDPMNLGMLGMHGCY
                     TSNRAVAECDVLIAVGTRFSDRVALNPKTFAKNATIIQIDIDASELGKNVDVDLSIVGD
                     AAYVLNAMLPQIKPAKHPDWMKMIQSWQAQDYHPVSDPTRLMPHQVIGEVCNQCGPDAV
                     YVTDVGQHQMWAAQYLRHTKSRGFITSGGLGTMGFGYGAAIGAQMALGRQQRVVMFTGD
                     ASFHMNLNEACTAVSYELPIITVIFNNSVLGMVRQWQTSFYGKRYSQTDPHRKTDFVKL
                     ADGFGLKGYRCTNLPEFQEAFADALQQKGPTWIECIIDKDEKVLPMIPGGGDINDIIME
                     "
ORIGIN
        1 tgccgctggc agcggtcgtc gctgttgctg ttcgaatcca cccgcccgat aagtttagcc
       61 ttgttacaaa acaaaaacct cagcgtactt gcatacgttg aggctttttg gtgggcgcgg
      121 gtggattcga accaccgaag ctgaaagcag cagatttaca gtctgtcccc attggccact
      181 cgggaacacg cccatattca gttgttgtgc atccgtgcgc cggacgactt gtttatttta
      241 gcatgagaaa agggttttgt caaccacctt ttttcacttt ttttcagcag gctcacagtc
      301 acctgcccgc cgcgcttcat ccggttgaat tgcgtcctgt tttatgttaa aataaggtaa
      361 agattctaca cattttacag gcaggagtgg gtcattgcat ggagtcaaca atcagcgccg
      421 ccccgcaaat ttctatcgac cgggaaaaga cgctgcgcag ccaccggcgt tactgggtgc
      481 tgcggcgtgc acaggacatc gtattctccc tgctggcgct gatcctgctt gcgccgatgg
      541 cactgctgat cagccttgcc atcgtgcttg acagccccgg agacggagct attttccggc
      601 agcggcgggt aggacgggac ggcaagctgt tctggctgta taagtttcgc accatgtgcc
      661 cggacgccga ggagcagctg aatgagctgc tgagccagaa ccagatggac ggcccggtgt
      721 tcaagatcga gggcgacccc cgcattaccc gggtgggacg ttttctgcgc aagaccagtc
      781 tggacgagct gccacagctg ctcaacgtgc tgcagggcga tatgagcatc gtggggccgc
      841 gccccgcact gccccgggag gtagagctgt acagcgacta ccagcgccag cggctgtacg
      901 tcacccccgg actgagctgc tactggcaga ttgcacccca ccgtaacgag atgagctttg
      961 acgagtgggt ggcgctggat ctgaaataca tccaggagcg cagcttctgg gtggactgga
     1021 agatcatctt cctcaccgtc cgcgccatgc tcatgaaata tggagagtga caaaagcgcc
     1081 tgttgtgcat ccgcacaaca ggcgcttttg ttacgtttat ttcagcaccg cacccagatt
     1141 tgcactggtg accatgcggg cgtaacggct cagccagccg gtcttgatgt tgggctcggg
     1201 cgcaacgtac tttgccttgc gctcggccag cactgcatcg ctgacctcca gcgtgatgga
     1261 ggcgttgggg atatcaatgg aaatggtatc cccttcttcg atcaggccga tggggccgcc
     1321 ggaggccgcc tccgggctga catggccgat ggcagcaccg cgggatgcgc cggagaagcg
     1381 gccgtctgtg atgagggcca ctgtcttatc cagcttcatg cctgccagcg cagaggtggg
     1441 gttgagcatc tcccgcatgc ccgggccgcc cttggggcct tcgtagcgga tgactaccac
     1501 atcgcccggg acgatcttgc ccgcgtagat ggcggcgatg gcctcctcct cgctgttgaa
     1561 cacccgcgct gggccggaat gctgctgcat ctccggggcc acggcgctgc gcttgaccac
     1621 acagccgtcc ggggcaatgt tgccccacag gatctgcaga ccgccggtct cagagtaggg
     1681 gttctcgatg ggacgaatgg ccttttcgtt caggatatgg gcacccttga tgttctcgcc
     1741 aagggtcttg ccggtgacgg tgggcacttc cagatccagc aggcccttct tggccagctc
     1801 cgcctgtact gccgggatgc cgcccgctgc gtacagatcc ggcatatggg tgggacctgc
     1861 cggtgccaga tggcacaggt tgggggtctt ggcgctgatc tcgttgaaca gcttcaggtc
     1921 gatgggcaca ccggcctcgt aggcgatagc cagcaggtgc agcaccgtgt tggtggagca
     1981 gcccagcgcc atatcacagg tcagtgcgtt gcggatggag cgctcgttga tgatctggcg
     2041 cgcggtgatg cccttgcgca ccatctccat cactgccata ccggcgtgct ttgccagctg
     2101 cagacgcttg gagtacacag ccgggatggt gccgttgccg ggcagggcga tgccgatggc
     2161 ctcgcacagg cagttcatgc tgttggcggt gtacataccg gagcagctgc cgcagctggg
     2221 acagcagttg caggtgcagt cctccagctg ctcgtcggtg atcataccgg ccttataggc
     2281 acccacggcc tcgaacatct tggaaaggct cacttcctcg ccgccgtaag taccggccag
     2341 catggggccg ccggagcaga ccacggcggg cacgtccatg cgcaccgctg ccatcaccat
     2401 gccgggcacg atcttatcgc agttgggcac cagcacaagg ccgtccagct ggtgtgccat
     2461 cagcatggtc tccacgctgt cgcagatcag ctcgcgggag gccagagagt acttcatgcc
     2521 cacatgaccc atggcgatgc catcgcatac accgatggcg gggatcagga tgggcgtacc
     2581 gccggccatc tgcacaccgg ccttgacagc ctcggcgatc ttatccagat tcatgtgtcc
     2641 gggcacgatc tcgctgtagg cgctcaccac gccgatgagg gggcgctcca gttcctccgg
     2701 ggtgtagccc agcgcataaa acaggctgcg gttgggcgcg cgctccgagc ccttggtcac
     2761 gttgtcactt ctcattgcaa atcctctttt ctgataaagc cctctccgtc acggctgcgc
     2821 cgtgccacct ctcccgaggg ggagaggttt tcgcggagga gctataaggt tcccttttca
     2881 acaaaaactc cccctttcgg gggagctgac cgctgaaaag cggtctgaga gggtttccct
     2941 cttacttctt cagccagctc atcatgctgc gcagctcagc acccaccttc tcgatgggca
     3001 gctcggcctc catacggcgc ttggcaagga agtgcacctt gcggccgccc ttggggctca
     3061 tctcggtcag gaactcggaa gcaaaggtgc cgtcctggat ctcggtcagg accttcttca
     3121 tctccttgcg ggtctcctcg gtgatcaggc gcttgccggt gcggtagtcg ccgtactcgg
     3181 cagtgttgga gatggagtag cgcatcttgg agaagccgcc ctcgttgatc aggtcaacga
     3241 tcagcttcat ctcgtggcag gtctcaaagt aggccatctc gggctcgtaa ccggcctcca
     3301 ccagcgtatc gaaaccggcg tggatcagct cggaaacgcc gccgcacagc acggcctgct
     3361 cgccgaacag atcggtctcg gtctcctcct tgaaggtggt ctgcaggatg cctgcacggc
     3421 ctgcgccgat gccggaagca taagccagag cgatgtcctt ggccttaccg gtgtagtcct
     3481 gctccacgca gatcagagag gggcagccct cgccctcggt gtacaggcgg cgcacgatgt
     3541 ggccggggcc cttgggggcg atcatgatga cgtccacgtt cttgggagcg gtgatggtct
     3601 tgaagtggat gttgaagccg tgtgcaaagg ccagtgcctt gccctcggtc atgtagggag
     3661 ccacctgcgt gttgtagata tcggcgcaca gttcatcggg caccagcatc atgacaacgt
     3721 caccggcctt ggcagcctct tccacttcca tcaccttgaa gttttcgtgg gtggcagcga
     3781 actcagaagc ctttgcccag tgggcagagc ccttgcgcag gccgaccaca acgttgacgc
     3841 cgctctcggc cagattctca gagtgtgcat gaccctggct gccgaagccg atgacagcga
     3901 cggtcttgcc gtccagcacg ccgaggttgc agtcggaatc gtagtatttc ttgatagcca
     3961 taatcgttag tctccttttg ctttttctgg ttggtttatc ttccggtctt gtatgctctg
     4021 ttctatcttt cgctcaccct gagcggaaaa gcaatgtgat ttatgcccgt cttgcgcccg
     4081 gcgggggcag ctggacggag cgcacctcct gcggcggctt ctggatatgc tggtgcatcc
     4141 cgccgcgctc cagtgcagta acgccggtgc ggcactgctc aaggatgttg tagccctcga
     4201 acattttcaa aaatgcgtcc agcttgtccg gccggccgat gagttcaaac accatactgt
     4261 ccggggaaag atcaatgatc tttgccttgt agatgcaagc gatctcccgc agctctgtgc
     4321 ggttgtgcac gtccgattcc accttgagca gcagcagctc ccgctccagc gctttttcgc
     4381 tgtccagcac gaacacctgc cgggtgactt ccagacgctc ggtctgcaat accagctgct
     4441 gcaaggcctt ttcatcgctg gtaatggtca ctgtgatgcg gctgactgcc gggtcgttgg
     4501 tggcggacac cgtaaggctg tcgatattga agccgcggcg gcagaacagg ctggacaccc
     4561 gggccagaac gccgctctgg ttgtccacca gcaggctgat gaccctgcgc tgatcagatt
     4621 ccatgatgct gtgtcctcct tattccatga tgatgtcgtt gatatcgccg ccgccgggga
     4681 tcataggcag caccttttcg tctttgtcga tgatgcactc gatccaggtg gggcctttct
     4741 gctgcagggc atctgcaaag gcttcctgaa actccggcag attggtgcag cggtagccct
     4801 tcaggccaaa gccgtctgcc agcttgacaa aatcggtctt gcggtggggg tcggtctggc
     4861 tgtaacgctt gccgtaaaag ctggtctgcc actggcgtac catgcccagc accgagttgt
     4921 tgaagatgac cgtgatgata ggcagctcgt agctgacggc ggtgcaggct tcgttcaggt
     4981 tcatgtggaa ggaggcgtca ccggtaaaca tcaccacacg ctgctggcgt cccagtgcca
     5041 tctgggcacc aatggcggca ccgtagccaa agcccatggt gccaaggccg ccgctggtga
     5101 taaagccgcg gctcttggtg tggcgcaggt actgcgccgc ccacatctgg tgctgaccca
     5161 catcggtaac atacacggcg tcggggccgc actggttgca cacctcgccg atgacctgat
     5221 ggggcatcag tcgggtgggg tcggacacag ggtggtaatc ctgtgcctgc cagctctgga
     5281 tcatcttcat ccagtccggg tgcttggcgg gcttgatctg gggcagcatg gcattcagca
     5341 cataggctgc atcgcccacg atggaaagat ccacatccac gttcttgccc agctcgctgg
     5401 catcaatatc gatttggatg atggtggcgt ttttggcaaa ggtcttgggg ttcagcgcca
     5461 cgcggtcgga aaagcgggtg cccaccgcga tgagcacatc acactccgct accgcccggt
     5521 tggaggtgta gcagccatgc atccccagca ttcccagatt catcgggtca ccatagggca
     5581 caacgcctgc ggccatcagg gtgtaagtgg cggggatctc cgccttgtgc agcagatccc
     5641 gcaagggctg gcagctggct gcgctgcgca cgccgccgcc aaagtatacc agcggacgct
     5701 gcgcggcgtt gatgaggtct gccgcccagt gcacctgttc ctcattatag gtagtcacgg
     5761 tgcggatggg ctccggctgc ttgggggtga actcgcacac tgctgcggtc acgtccttgg
     5821 ggatatccac cagcaccggg
//
