LOCUS       c00338_gnlPro..         3949 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AMEPOAEB_338.
ACCESSION   c00338_gnlPro..
VERSION     c00338_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 07:24:58
            Original ID  :: gnl|Prokka|AMEPOAEB_338
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3949
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3949
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[181:3947]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      182..3947
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..3949
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3949
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             2..166
                     /locus_tag="ctg338_1"
                     /transl_table=11
                     /translation="PCMDCKRVTVIRIGPVISTRMKALHGNVWCFFVLSAVHLTSPFMK
                     KAFVAARRP"
     CDS             complement(182..1393)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg338_2"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 4.7e-15,
                     bitscore: 44.2, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVRDSRPPFAGLANLSEAQLHRLPTPCYLLDEAQLCRNGEILLG
                     VQQRTGCRILLAQKAFSNFNVYPVLAPYLAGTEASGLYESRLGREELPEKENHVFCAAY
                     RTDEFEELLQYADHIVFNSPSQLAKFGPAAKAAGKSIGLRINPECSTQEGHEIYDPCAP
                     GSRLGTTRAQWDAAVAAHPELPALLDGLHFHTLCEQDADALAVTLAAVEKKFADLLPRM
                     QWLNFGGGHHITRPGYALATLENCILSVRQKYGVQVYLEPGEAWALNAGYLVTTVLDTL
                     RNGDTSLAILDMSAACHTPDVIEMPYRPPLLDAGEAGEKAHTVRLGGPTCLAGDIIGDY
                     SFDTPLAEGDKLIFGDMAIYTTCKNNTFNGMPLPPIWLLGADGNCRELVHFGYQDFKMR
                     LGAQ"
     CDS             complement(1393..2652)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg338_3"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 1.4e-45, bitscore:
                     144.9, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 5e-34, bitscore:
                     105.9, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKVLIIGCGGVASVAIHKCCQVPEVFTEICIASRTKAKCDKLAA
                     ELAPTTTTKITTAQVDADKVEEVIALIKAYQPDLVMNIALPYQDLTIMDACLACGVNYM
                     DTANYEPENTDDPEWRAIYEKRCKEAGFSAYFDYSWQWAYAKKFEEAGLTALLGSGFDP
                     GVTQAYCAYAKKHEFDTIDTIDILDCNGGDHGYAFATNFNPEINLREVSAPGSYWENGH
                     WVEIPAMSIKREYNFDQVGDKDMYLLHHEEIESLAKNIPEAKRIRFFMTFGQSYLDHMR
                     CLEDVGMLSTTPVNFNGQEIVPIQFLKALLPDPASLGPRTKGKTNIGCIFTGKKDGKDK
                     TYYIYNVCDHQECYKEVGSQAISYTTGVPAMCGALMLLTGKWTTKGVHTVEEFDPDPYL
                     DALDKYGLPRSESHNPALVD"
     CDS             complement(2692..3567)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Arginase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg338_4"
                     /sec_met_domain="Arginase (E-value: 3e-78, bitscore: 251.5,
                     seeds: 235, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHPNVETFIGCDSSYRAASIVLYGAPYDSTTSYRPGARFGPAAIR
                     HESYGLETYSPYQNADLTDFDVFDSGDLELCFGSSELALADIEARAEEILKDGKFPLLL
                     GGEHLVTLGAVRAAVKKYPDLHIVHFDAHADLRDDYLGAKLSHACVLRRCHELVGDGHI
                     HQFCIRSGDRAEFEFAAQHTEMHKFDFTGLAELTAQLCESKVPVYLTIDLDCLDPSCFP
                     GTGTPEAGGVSFLQLLEAIRTVTKANIIGADLNELAPTLDTTGVSTATACKVLRETLIA
                     LDKGWPGFQV"
     CDS             complement(3567..3947)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg338_5"
                     /sec_met_domain="Spermine_synth (E-value: 1.1e-14,
                     bitscore: 42.7, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FGPSEGLFTREFYGSCFNALKEDGIMVNQQGSPFYAEDASAMQRS
                     HKRIASTFPISRVYQAHIPTFAAGYWLFGFASKKYHPIDDLDAESWKALNMRTRYYTTK
                     LHIGAFYLPAFLEEMLREVEEH"
ORIGIN
        1 gccgtgcatg gactgcaaga gggtcaccgt gatcagaatc ggcccggtaa tctccaccag
       61 aatgaaagca ctacacggaa acgtgtggtg cttttttgtt ttatctgccg ttcaccttac
      121 gtcccctttc atgaaaaaag cgtttgtcgc agcccgcagg ccgtgacaaa cgctttttat
      181 tttactgtgc gcccaagcgc attttgaagt cctgatagcc aaagtgcacc agctcccggc
      241 aattgccgtc tgcgcccagc agccagatag gcggcagggg catcccattg aaggtgttgt
      301 tcttgcaggt ggtgtagatg gccatatcgc cgaaaatgag cttatcgccc tcggcaaggg
      361 gggtgtcaaa gctgtaatcc ccgatgatat cccccgcaag acaggtgggg ccgcccagcc
      421 ggacggtgtg cgccttctct cctgcctcgc cggcgtccag cagcggcggg cggtagggca
      481 tctcgatgac atccggggtg tggcaggctg cggacatatc cagaatggca agactggtat
      541 ccccgttgcg cagggtgtcc agcacggtag tgaccagata gcctgcattc agcgcccaag
      601 cctcgccggg ctccaaatac acctgtacgc cgtacttctg ccgcaccgag aggatgcagt
      661 tttccagcgt agcaagggca tagcccgggc gggtgatgtg gtgtcccccg ccgaaattga
      721 gccactgcat tctgggcagc aggtcggcaa actttttctc cactgcggcc agcgttaccg
      781 ccagcgcatc ggcgtcctgc tcgcacaggg tatggaagtg cagaccgtcc aacagggcgg
      841 gcagctccgg gtgcgctgcc acagcggcgt cccactgagc acgggtggtg cccagacggc
      901 tgccgggggc gcaggggtcg taaatttcat gaccctcctg cgtggagcac tccgggttga
      961 tgcgcagacc gatgcttttg cccgccgcct ttgccgccgg gccgaacttt gccagctggc
     1021 tgggcgagtt gaacacgatg tggtcggcgt attgcagcaa ttcctcaaac tcatctgtcc
     1081 ggtaggcggc gcagaacaca tggttctcct tctccggcag ctcttccctg cccaagcggc
     1141 tctcgtacag gccgctggcc tcggtacctg ccagataggg tgccagcacc gggtagacgt
     1201 taaagttgga aaaagccttc tgcgccagca ggatgcggca gccggtgcgc tgctgcacac
     1261 ccagcaggat ctcgccgttg cggcacagtt gggcttcgtc cagcagatag cagggggtgg
     1321 gcaggcggtg cagctgcgcc tcggacaggt ttgccagccc cgcaaagggc gggcggctgt
     1381 cgcgcacccg catcagtcca ccagagccgg gttatggctc tcggagcggg gcaggccgta
     1441 cttatccagt gcatccagat acggatcggg atcaaactcc tcgacagtgt gcacgccctt
     1501 ggtggtccac ttgccggtga gcagcatcag ggcaccgcac atggcgggca cgccggtggt
     1561 gtagctgata gcctgagagc ccacttcctt gtagcactcc tgatggtcgc agacgttgta
     1621 gatatagtag gtcttatcct tgccgtcctt tttgccggta aagatgcagc cgatgttggt
     1681 cttgcctttg gtacgggggc caaggctggc ggggtccggc agcagagcct tgaggaactg
     1741 gatgggcacg atctcctgtc cgttgaagtt gacaggggtg gtggacagca tgcccacgtc
     1801 ctccagacaa cgcatgtggt ccagatagct ctggccaaag gtcatgaaga agcggatgcg
     1861 ctttgcctcc gggatgttct ttgccagaga ctcgatctcc tcgtggtgca gcaggtacat
     1921 atccttgtcg cccacctgat cgaagttgta ctcccgcttg atgctcatag ccgggatctc
     1981 tacccagtgg ccgttctccc agtagctgcc gggagcggac acctcgcgca ggttgatctc
     2041 cgggttaaag ttagtggcaa aggcatagcc gtggtcgccg ccgttgcagt ccagaatatc
     2101 gatggtgtca atggtatcga actcgtgctt tttggcatag gcacagtaag cctgggtcac
     2161 acccgggtcg aagccgctgc ccagcagggc ggtcaggccg gcttcctcga actttttggc
     2221 gtaggcccac tgccagctgt aatcaaagta ggcagagaag ccggcctcct tgcagcgctt
     2281 ctcgtagatg gcgcgccact ccgggtcatc ggtgttctcg ggctcgtagt tggcggtgtc
     2341 catgtagttg acgccgcagg caaggcaggc gtccatgatg gtcagatcct gataaggcag
     2401 ggcgatgttc atcacgaggt cgggctggta agccttgatg agggcgatga cctcctccac
     2461 cttgtctgcg tccacctgtg cggtggtgat cttggtagta gtggtgggag ccagctcagc
     2521 agccagctta tcgcactttg ccttggtacg gctggcgatg cagatctcgg tgaacacctc
     2581 gggcacctgg cagcacttat ggatggcaac agaggccacg ccgccgcagc cgatgatcag
     2641 aactttgctc atgtcggtta ctcctttatc tgtaaaatgg taggttcgta tttatacctg
     2701 aaagcccggc cagcccttgt ccagcgcgat cagggtctcg cgcagcacct tgcaggcggt
     2761 ggcggtggaa acgccggtgg tgtccagcgt gggggcaagc tcgttcaggt ctgcaccgat
     2821 gatgttggcc ttggtcacag tgcggatggc ctccagcagc tgcaaaaagc ttacgccgcc
     2881 agcctccggg gtaccggtgc ccgggaagca ggaggggtca aggcagtcca gatcgatggt
     2941 caggtacacc ggcaccttgc tctcgcacag ctgtgcggtc agctcggcaa ggccggtaaa
     3001 atcgaacttg tgcatctcgg tgtgctgggc agcaaactca aattctgccc ggtcgccgct
     3061 gcggatgcag aactgatgga tgtggccatc ccctaccagc tcgtggcagc ggcgcagcac
     3121 acaagcgtgg ctgagcttcg cgcccagata gtcgtcccgc aggtcggcgt gggcgtcgaa
     3181 atggacgatg tgcaggtcgg ggtatttctt caccgcagca cgcaccgcac ccagcgtgac
     3241 caaatgctcg ccacccagca gcagcgggaa cttgccgtcc ttcaaaattt cctctgcccg
     3301 ggcctcgata tcggccagtg ccagctcgct ggagccgaag cacagctcca gatcgccgct
     3361 gtcgaacaca tcaaaatcgg tcaggtcggc gttctggtaa gggctgtaag tctccagacc
     3421 gtagctctcg tgccggatgg ccgccgggcc gaaccgtgca ccggggcggt agctggtggt
     3481 ggaatcgtaa ggcgcaccgt acagcacgat gctggcggcg cggtagctgc tgtcgcagcc
     3541 gataaaggtc tcaacgttgg ggtgcatcag tgttcctcca cttcccgcag catctcctcc
     3601 agaaaagccg gcaggtagaa tgctccgata tgcagttttg tggtgtagta gcgggtgcgc
     3661 atattcagtg ccttccacga ttcagcgtcc agatcatcga tgggatgata ttttttgctt
     3721 gcaaagccga acagccagta ccctgccgca aaggtgggga tatgcgcctg atacacacgg
     3781 ctgatgggga aggtggatgc gatgcgctta tggctgcgct gcatggcgct ggcgtcctcg
     3841 gcgtagaagg ggctgccctg ctggttgacc ataatgccgt cctctttcag ggcgttgaag
     3901 cagctgccgt agaactcgcg ggtgaacaga ccctcagagg ggccgaagg
//
