GCA_019013225.1 - 9 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaBNLONLDD_10 (original name was: gnl|Prokka|BNLONLDD_10)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	cyclic-lactone-autoinducer	1	10,991

gnlProkkaBNLONLDD_41 (original name was: gnl|Prokka|BNLONLDD_41)

Region	Type	From	To	Most similar known cluster		Similarity
Region 41.1	lanthipeptide-class-ii	1	21,265

gnlProkkaBNLONLDD_47 (original name was: gnl|Prokka|BNLONLDD_47)

Region	Type	From	To	Most similar known cluster		Similarity
Region 47.1	ranthipeptide	22,334	55,808

gnlProkkaBNLONLDD_112 (original name was: gnl|Prokka|BNLONLDD_112)

Region	Type	From	To	Most similar known cluster		Similarity
Region 112.1	RRE-containing	1	5,960

gnlProkkaBNLONLDD_129 (original name was: gnl|Prokka|BNLONLDD_129)

Region	Type	From	To	Most similar known cluster		Similarity
Region 129.1	ranthipeptide	45,757	59,440

gnlProkkaBNLONLDD_130 (original name was: gnl|Prokka|BNLONLDD_130)

Region	Type	From	To	Most similar known cluster		Similarity
Region 130.1	cyclic-lactone-autoinducer	13,644	25,832

gnlProkkaBNLONLDD_142 (original name was: gnl|Prokka|BNLONLDD_142)

Region	Type	From	To	Most similar known cluster		Similarity
Region 142.1	cyclic-lactone-autoinducer	8,412	20,233

gnlProkkaBNLONLDD_152 (original name was: gnl|Prokka|BNLONLDD_152)

Region	Type	From	To	Most similar known cluster		Similarity
Region 152.1	NRPS	1	23,735	dipeptide aldehydes	NRP	35%

gnlProkkaBNLONLDD_194 (original name was: gnl|Prokka|BNLONLDD_194)

Region	Type	From	To	Most similar known cluster		Similarity
Region 194.1	cyclic-lactone-autoinducer	1	6,351

Region	Type	From	To	Most similar known cluster	Similarity
Region 10.1	cyclic-lactone-autoinducer	1	10,991
Region 41.1	lanthipeptide-class-ii	1	21,265
Region 47.1	ranthipeptide	22,334	55,808
Region 112.1	RRE-containing	1	5,960
Region 129.1	ranthipeptide	45,757	59,440
Region 130.1	cyclic-lactone-autoinducer	13,644	25,832
Region 142.1	cyclic-lactone-autoinducer	8,412	20,233
Region 152.1	NRPS	1	23,735	dipeptide aldehydes	NRP	35%
Region 194.1	cyclic-lactone-autoinducer	1	6,351

Compact view

gnlProkkaBNLONLDD_10 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 10,991 nt. (total: 10,991 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaBNLONLDD_41 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 21,265 nt. (total: 21,265 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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Lanthipeptides

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Lanthipeptide predictions

Lanthipeptide(s) for ctg41_9

allorf_00092_00277

Leader:

MSLNFSTYAYITKSPVRYSDAAVFSA

Core:

AAPDYHHPELFPVLLLFHTRELYPHMTEVTCFQTL

Core with dehydrations:

AAPDYHHPELFPVLLLFHDhbRELYPHMDhbEVDhbCysFQDhbL

antiSMASH-DB 4.0 matches for allorf_00092_00277

14.3% NZ_CP058552.1: Lanthipeptides: allorf_1481579_1481740_lanthipeptide

23

YPHMTEVTCFQTL

35

Y H+ + C QT+

6

YKHVQKTNCVQTI

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_00092_00277 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 4093.1 Da

Molecular weight: 4095.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4113.7 Da N = 1; 4131.7 Da N = 2; 4149.7 Da N = 3

gnlProkkaBNLONLDD_47 - Region 1 - ranthipeptide

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Location: 22,334 - 55,808 nt. (total: 33,475 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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gnlProkkaBNLONLDD_112 - Region 1 - RRE-containing

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Location: 1 - 5,960 nt. (total: 5,960 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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gnlProkkaBNLONLDD_129 - Region 1 - ranthipeptide

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Location: 45,757 - 59,440 nt. (total: 13,684 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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gnlProkkaBNLONLDD_130 - Region 1 - cyclic-lactone-autoinducer

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Location: 13,644 - 25,832 nt. (total: 12,189 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

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gnlProkkaBNLONLDD_142 - Region 1 - cyclic-lactone-autoinducer

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Location: 8,412 - 20,233 nt. (total: 11,822 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

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resistance

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gnlProkkaBNLONLDD_152 - Region 1 - NRPS

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Location: 1 - 23,735 nt. (total: 23,735 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 23735:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg152_10

AMP-binding (22..421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1035..1423): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2029..2420): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaBNLONLDD_194 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 6,351 nt. (total: 6,351 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

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resistance

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