LOCUS       c00036_gnlPro..         3977 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GAICKDJP_36.
ACCESSION   c00036_gnlPro..
VERSION     c00036_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 09:17:37
            Original ID  :: gnl|Prokka|GAICKDJP_36
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3977
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3977
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[941:3897]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      942..3897
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3977
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3977
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             942..3170
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg36_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1170.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             3172..3897
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg36_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.6e-32, bitscore:
                     98.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANDIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
ORIGIN
        1 tgcaatacgt aaacttcatt aacgacaagc ccattggcag aattaagtat gcattaaatt
       61 aattccgcca acaggtaaac attatatatt atcatgacag accctgattt attatttact
      121 gcaaagatac gaaatcttac ggaaaatgaa ttggttgttt ttttcgtttg ttctgttttt
      181 tatcaaatta ttttccgagc gaatatgatt cgtcttaaat aaaataaaaa cacccaactt
      241 tacattcttg ggtgtaaaat tgggtgtttg ttttataatt tgctgatttt cagcgtttat
      301 tgcggagaga cagggattcg aaccccgggt gcctcgcagc acaacggttt tcaagaccgc
      361 cgcaatcgac cactctgcca cctctccaaa acttcttttt cagaagtact tttcttttaa
      421 agcggtgcaa agatacaaat cattttttat tctacaaact tttctgcaca aaaacagagc
      481 aattatacca aaattcttgt ttatacccac tacggaacga acataacggc atttaggcta
      541 tttgttgaaa tttatcaagg gcaagacaga gttttataac ttactggcta tcagtatatt
      601 tacaaagagc cacattcagt atacgattga accctattca gtatacgact gaatggcatt
      661 cactatacga ctgaattagg ttcagaatac gactgttcct taaacctaca tcatttgtaa
      721 ataatgcaga ttattattcg gttcttgttt caattcataa accacatata aagaaagtat
      781 taacatctct actcccccgg acattcacta cctaataata gttattttat gctgttcaac
      841 ataaaaagag aagtatctct tataaaatta cggaatttat tcagtatttt tgcgcgctct
      901 aaaggataac attatctaac ctactaacaa aaacaaatat tatggaattg aacaaaacct
      961 ttaccaatgg gctttggaat aaagaaatca atgtcagtga tttcgtcagt aaaaacatcg
     1021 caccctatac cggggatgca tctttcttgc agggacctac cgaacgtacc aaacgtatct
     1081 gggacatctg tctgaaggct ctcgaagaag aaaggaacaa caatggcgtc cgttcactgg
     1141 acaataccac cgtatctacc atcacttctc ataaagccgg gtatattgat aaggacaatg
     1201 aacttattgt cggtttgcag actgatgaac tgttgaaacg atccatcaaa cctttcggag
     1261 gcatcaacgt ggtagcaaaa gcatgccgtg aaaacggagt ggaagtggac gataaagtaa
     1321 aagacatctt cacccattac cgcaaaacac ataacgacgg cgtattcgat gtatataccg
     1381 aagaaatccg ttccttccgc tcactgggat tccttaccgg attgcccgac aactatgccc
     1441 gcggacgtat catcggcgac taccgccgat tggctctcta cggcatcgac cgtctgattg
     1501 aagccaagca ggaggacttg cgcaacctca ccggcccgat gaccgaagca cgcatccgtc
     1561 tacgcgaaga agtggcagaa caaatcaaag ctttgaagga catcaaggtt atgggcgaat
     1621 attacggact cgacctcagc catccggcta catcagctca agaggccgtg caatgggtat
     1681 acatggcata tcttgctgcc atcaaagagc aggacggagc agccatgtca ctgggtaacg
     1741 tatcttcgtt cctcgacatt ttcatcgaat acgatttggc tcacggcaag attgacgaga
     1801 cgtttgcaca ggaactgata gaccagttcg tcatcaaact acgtatggta cgccatctgc
     1861 gcatgcagtc gtacaatgat atctttgccg gcgacccgac ttgggtgacc gaagccatcg
     1921 gcggacgctt caacgacgga cgcgtgaagg tcaccaagac ttctttccgc tttttgcaga
     1981 cgctatacaa cctcggtcct tctccggaac cgaacttgac cgtattgtgg agtcccgaac
     2041 tgccggaagg cttcaaggat ttctgcgcca aagtttctgt agataccagc tccatccagt
     2101 acgaaaacga caacctgatg cgcgaagtgc gcaactgcga cgattacggc attgcctgct
     2161 gtgtatccta ccaagccatc ggcaagcaaa tccagttctt cggcgcacgt gccaaccttg
     2221 ccaaggccct gctgcttgcc atcaacggtg gccgttgcga aaacaccgga actgtcatgg
     2281 tgaaaggtat tcccgtattg accggcgaga cactgaagtt tgaggaagtt atggccaact
     2341 acaagaaggt actgactgaa atagcccgtg tctacaacga ggccatgaat atcatccact
     2401 acatgcatga taagtattat tatgagaaag cccagatggc attcatcgat accgacccac
     2461 gcatcaatct ggcatacggt gttgccggcc tttccatcgc catagactca ctgtccgcca
     2521 tcaaatatgc caaagtcact gcacgccgca acgaaatcgg cctgaccgaa ggtttcgaca
     2581 ccgaaggctc attcccctgc ttcggcaata atgacgaccg tgtggaccac ctgggagtag
     2641 acctcgttta ctatttcagc gaagaactga aaaagctgcc tgtctacaag aatgcccgtc
     2701 ccaccctttc cttgctgact atcacctcca acgtgatgta cggcaagaag accggtgcca
     2761 ctccggacgg acgcgccaaa ggtgttgcct ttgctccggg tgccaaccca atgcacggac
     2821 gtgacaagaa cggtgccatc gcttccttga gctccgtagc caaattgcgc taccgcgact
     2881 cgcaggacgg catcagcaat acgttctcca ttgttcccaa atcactgggc ccgacaccgg
     2941 aagaacgtgt agagaacctg gtaaccatga tggacggcta cttcaccaaa ggtgcccacc
     3001 atttgaacgt aaacgtactg aaccgcgaaa tgctggaaga tgccatggaa catcctgaaa
     3061 agtatccgca actcaccatc cgcgtttccg gctatgccgt gaacttcgtg aagttgagcc
     3121 gcgagcatca gttggaagtc atcagccgtt cattccacga aagaatgtaa tatgataaat
     3181 gtacactcct acgaaagtat gggaacattc gacgggccgg gtctccggct cgtcgttttc
     3241 ctgcaaggat gtccgttccg ttgtctgtat tgtgccaatc cggatacgat tgatgtaaaa
     3301 ggaggcactc ccaccactcc ggaagagatt ctgcaaatgg ctatcagcca aaaggcattc
     3361 ttcgggaaga aaggaggtat tactttctcc ggcggtgagc ctaccctgca agcagaagcg
     3421 ctgattcctc ttttcaagga gctgaaggcg aacgacatcc atatctgtct ggacagtaac
     3481 ggaggcatct ggaatgaaaa ggtggaagag ctctttagtc tgactgacct cgtgctgctg
     3541 gacatcaagc aattcaatcc cgaacgccac cgtacgctga cgggacgaag caacgaacag
     3601 acactccgca ccgctgcctg gctcgaagaa catgaacacc ccttctggct gcgctatgta
     3661 ctggtccccg gttacagcga ttcggaagaa gacacccgcc tgctgggtga aagtcttggc
     3721 aaataccagc agatacaacg ggtggaaata cttccttatc accgtctggg tgttcacaaa
     3781 tacgaagcca tgggatggga ttacaaattg aaagaagtga aggaaaatac tccggaacaa
     3841 ctggaacggg cagaaaaact gttcaaagag tatttcacga atgtgataac caactaaaac
     3901 gaggatgtaa attaaactgt gtcagcaagg aataaagtat taactttgct aacacagttt
     3961 tttttatgaa agaagaa
//
