GCA_019132815.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaNOEPNLEE_46 (original name was: gnl|Prokka|NOEPNLEE_46)

Region	Type	From	To	Most similar known cluster		Similarity
Region 27.1	RRE-containing	449	20,793

gnlProkkaNOEPNLEE_78 (original name was: gnl|Prokka|NOEPNLEE_78)

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	NRPS	19,542	65,706

gnlProkkaNOEPNLEE_81 (original name was: gnl|Prokka|NOEPNLEE_81)

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	resorcinol	24,857	65,996

gnlProkkaNOEPNLEE_112 (original name was: gnl|Prokka|NOEPNLEE_112)

Region	Type	From	To	Most similar known cluster		Similarity
Region 69.1	phosphonate	28,262	42,575	polysaccharide B	Saccharide	13%

Region	Type	From	To	Most similar known cluster	Similarity
Region 27.1	RRE-containing	449	20,793
Region 35.1	NRPS	19,542	65,706
Region 38.1	resorcinol	24,857	65,996
Region 69.1	phosphonate	28,262	42,575	polysaccharide B	Saccharide	13%

Compact view

gnlProkkaNOEPNLEE_46 - Region 1 - RRE-containing

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Location: 449 - 20,793 nt. (total: 20,345 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNOEPNLEE_78 - Region 1 - NRPS

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Location: 19,542 - 65,706 nt. (total: 46,165 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 19541 - 65706:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg35_36

AMP-binding (8..447): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYFGSGSLIK
Nearest Stachelhaus code(s):: Ala DMGGFGCLIK (50% 8Å match); Leu DYFTFGLIIK (41% 8Å match); Hpr DYQYCGHLIK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1210..1629): X

nrpys: Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMTNMGEVAK
Nearest Stachelhaus code(s):: Asn DMTKVGEVGK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

gnlProkkaNOEPNLEE_81 - Region 1 - resorcinol

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Location: 24,857 - 65,996 nt. (total: 41,140 nt) Show pHMM detection rules used

resorcinol: DarB

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNOEPNLEE_112 - Region 1 - phosphonate

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Location: 28,262 - 42,575 nt. (total: 14,314 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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core biosynthetic genes

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resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1